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Q95M18

- ENPL_BOVIN

UniProt

Q95M18 - ENPL_BOVIN

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Protein
Endoplasmin
Gene
HSP90B1, GRP94, TRA1
Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Molecular chaperone that functions in the processing and transport of secreted proteins. When associated with CNPY3, required for proper folding of Toll-like receptors. Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei107 – 1071ATP By similarity
Binding sitei149 – 1491ATP By similarity
Binding sitei168 – 1681ATP By similarity
Binding sitei199 – 1991ATP; via amide nitrogen By similarity
Binding sitei448 – 4481ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. RNA binding Source: AgBase
  3. low-density lipoprotein particle receptor binding Source: AgBase
  4. virion binding Source: Ensembl

GO - Biological processi

  1. ER-associated ubiquitin-dependent protein catabolic process Source: UniProtKB
  2. actin rod assembly Source: Ensembl
  3. cellular response to ATP Source: Ensembl
  4. negative regulation of apoptotic process Source: AgBase
  5. protein folding Source: InterPro
  6. regulation of phosphoprotein phosphatase activity Source: Ensembl
  7. response to hypoxia Source: AgBase
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Ligandi

ATP-binding, Calcium, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_209041. Scavenging by Class A Receptors.
REACT_224406. ATF6-alpha activates chaperone genes.
REACT_225126. Trafficking and processing of endosomal TLR.

Names & Taxonomyi

Protein namesi
Recommended name:
Endoplasmin
Alternative name(s):
94 kDa glucose-regulated protein
Short name:
GRP-94
Heat shock protein 90 kDa beta member 1
Gene namesi
Name:HSP90B1
Synonyms:GRP94, TRA1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 5

Subcellular locationi

Endoplasmic reticulum lumen By similarity. Melanosome By similarity UniRule annotation

GO - Cellular componenti

  1. cytosol Source: AgBase
  2. endoplasmic reticulum Source: AgBase
  3. endoplasmic reticulum lumen Source: UniProtKB-SubCell
  4. endoplasmic reticulum membrane Source: Ensembl
  5. melanosome Source: UniProtKB-SubCell
  6. midbody Source: Ensembl
  7. perinuclear region of cytoplasm Source: AgBase
  8. plasma membrane Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121 Reviewed prediction
Add
BLAST
Chaini22 – 804783EndoplasminUniRule annotation
PRO_0000013596Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi62 – 621N-linked (GlcNAc...) Reviewed prediction
Glycosylationi107 – 1071N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi138 – 138Interchain By similarity
Glycosylationi217 – 2171N-linked (GlcNAc...) Reviewed prediction
Modified residuei306 – 3061Phosphoserine By similarity
Modified residuei404 – 4041N6-succinyllysine By similarity
Glycosylationi445 – 4451N-linked (GlcNAc...) Reviewed prediction
Modified residuei479 – 4791N6-acetyllysine By similarity
Glycosylationi481 – 4811N-linked (GlcNAc...) Reviewed prediction
Glycosylationi502 – 5021N-linked (GlcNAc...) Reviewed prediction
Modified residuei633 – 6331N6-succinyllysine By similarity

Post-translational modificationi

Phosphorylated By similarity.UniRule annotation

Keywords - PTMi

Acetylation, Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ95M18.
PRIDEiQ95M18.

Interactioni

Subunit structurei

Homodimer; disulfide-linked. Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5 By similarity. Part of a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX. Interacts with AIMP1; regulates its retention in the endoplasmic reticulum. Interacts with OS9 By similarity. Interacts with CNPY3; this interaction is disrupted in the presence of ATP. Interacts with several TLRs, including TLR4 and TLR9, but not with TLR3 By similarity. Interacts with MZB1 in a calcium-dependent manner By similarity. Interacts with METTL23 By similarity.

Protein-protein interaction databases

IntActiQ95M18. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ95M18.
SMRiQ95M18. Positions 69-755.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi801 – 8044Prevents secretion from ER Reviewed prediction

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0326.
GeneTreeiENSGT00750000117672.
HOGENOMiHOG000031988.
HOVERGENiHBG007374.
InParanoidiQ95M18.
KOiK09487.
OMAiQSSHHPS.
OrthoDBiEOG780RM0.
TreeFamiTF105969.

Family and domain databases

Gene3Di3.30.565.10. 2 hits.
HAMAPiMF_00505. HSP90.
InterProiIPR003594. HATPase_ATP-bd.
IPR019805. Heat_shock_protein_90_CS.
IPR001404. Hsp90_fam.
IPR020575. Hsp90_N.
IPR020568. Ribosomal_S5_D2-typ_fold.
[Graphical view]
PANTHERiPTHR11528. PTHR11528. 1 hit.
PfamiPF00183. HSP90. 1 hit.
[Graphical view]
PIRSFiPIRSF002583. Hsp90. 1 hit.
PRINTSiPR00775. HEATSHOCK90.
SMARTiSM00387. HATPase_c. 1 hit.
[Graphical view]
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55874. SSF55874. 2 hits.
PROSITEiPS00014. ER_TARGET. 1 hit.
PS00298. HSP90. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q95M18-1 [UniParc]FASTAAdd to Basket

« Hide

MRALWVLGLC CVLLTFGSVR ADDEVDVDGT VEEDLGKSRE GSRTDDEVVQ    50
REEEAIQLDG LNASQIRELR EKSEKFAFQA EVNRMMKLII NSLYKNKEIF 100
LRELISNASD ALDKIRLISL TDENALAGNE ELTVKIKCDK EKNLLHVTDT 150
GVGMTREELV KNLGTIAKSG TSEFLNKMTE AQEDGQSTSE LIGQFGVGFY 200
SAFLVADKVI VTSKHNNDTQ HIWESDSNEF SVIADPRGNT LGRGTTITLV 250
LKEEASDYLE LDTIKNLVKK YSQFINFPIY VWSSKTETVE EPAEEEEAAK 300
EDKEESDDEA AVEEEEDEKK PKTKKVEKTV WDWELMNDIK PIWQRPSKEV 350
EEDEYKAFYK SFSKESDDPM AYIHFTAEGE VTFKSILFVP TSAPRGLFDE 400
YGSKKSDYIK LYVRRVFITD DFHDMMPKYL NFVKGVVDSD DLPLNVSRET 450
LQQHKLLKVI RKKLVRKTLD MIKKIADEKY NDTFWKEFGT NIKLGVIEDH 500
SNRTRLAKLL RFQSSHHPSD MTSLDQYVER MKEKQDKIYF MAGASRKEAE 550
SSPFVERLLK KGYEVIYLTE PVDEYCIQAL PEFDGKRFQN VAKEGVKFDE 600
SEKSKESREA VEKEFEPLLN WMKDKALKDK IEKAVVSQRL TESPCALVAS 650
QYGWSGNMER IMKAQAYQTG KDISTNYYAS QKKTFEINPR HPLIRDMLRR 700
VKEDEDDKTV SDLAVVLFET ATLRSGYLLP DTKAYGDRIE RMLRLSLNID 750
PDAKVEEEPE EEPEETTEDT AEDTEQDEEE EMDAGTDEEE QETAEKSTAE 800
KDEL 804
Length:804
Mass (Da):92,427
Last modified:December 1, 2001 - v1
Checksum:i0A204A77E61F1FDE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB025193 mRNA. Translation: BAB69766.1.
BC104549 mRNA. Translation: AAI04550.1.
RefSeqiNP_777125.1. NM_174700.2.
UniGeneiBt.8686.

Genome annotation databases

EnsembliENSBTAT00000004364; ENSBTAP00000004364; ENSBTAG00000003362.
GeneIDi282646.
KEGGibta:282646.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB025193 mRNA. Translation: BAB69766.1 .
BC104549 mRNA. Translation: AAI04550.1 .
RefSeqi NP_777125.1. NM_174700.2.
UniGenei Bt.8686.

3D structure databases

ProteinModelPortali Q95M18.
SMRi Q95M18. Positions 69-755.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q95M18. 1 interaction.

Proteomic databases

PaxDbi Q95M18.
PRIDEi Q95M18.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSBTAT00000004364 ; ENSBTAP00000004364 ; ENSBTAG00000003362 .
GeneIDi 282646.
KEGGi bta:282646.

Organism-specific databases

CTDi 7184.

Phylogenomic databases

eggNOGi COG0326.
GeneTreei ENSGT00750000117672.
HOGENOMi HOG000031988.
HOVERGENi HBG007374.
InParanoidi Q95M18.
KOi K09487.
OMAi QSSHHPS.
OrthoDBi EOG780RM0.
TreeFami TF105969.

Enzyme and pathway databases

Reactomei REACT_209041. Scavenging by Class A Receptors.
REACT_224406. ATF6-alpha activates chaperone genes.
REACT_225126. Trafficking and processing of endosomal TLR.

Miscellaneous databases

NextBioi 20806327.

Family and domain databases

Gene3Di 3.30.565.10. 2 hits.
HAMAPi MF_00505. HSP90.
InterProi IPR003594. HATPase_ATP-bd.
IPR019805. Heat_shock_protein_90_CS.
IPR001404. Hsp90_fam.
IPR020575. Hsp90_N.
IPR020568. Ribosomal_S5_D2-typ_fold.
[Graphical view ]
PANTHERi PTHR11528. PTHR11528. 1 hit.
Pfami PF00183. HSP90. 1 hit.
[Graphical view ]
PIRSFi PIRSF002583. Hsp90. 1 hit.
PRINTSi PR00775. HEATSHOCK90.
SMARTi SM00387. HATPase_c. 1 hit.
[Graphical view ]
SUPFAMi SSF54211. SSF54211. 1 hit.
SSF55874. SSF55874. 2 hits.
PROSITEi PS00014. ER_TARGET. 1 hit.
PS00298. HSP90. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of bovine (Bos taurus) cDNA encoding a 94-kDa glucose-regulated protein and developmental changes in its mRNA and protein content in the mammary gland."
    Watanabe A., Uchida I., Nakata K., Fujimoto Y., Oikawa S.
    Comp. Biochem. Physiol. 130B:547-557(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Mammary gland.
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Uterus.

Entry informationi

Entry nameiENPL_BOVIN
AccessioniPrimary (citable) accession number: Q95M18
Secondary accession number(s): Q3MHX8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2003
Last sequence update: December 1, 2001
Last modified: September 3, 2014
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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