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Q95M18

- ENPL_BOVIN

UniProt

Q95M18 - ENPL_BOVIN

Protein

Endoplasmin

Gene

HSP90B1

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 108 (01 Oct 2014)
      Sequence version 1 (01 Dec 2001)
      Previous versions | rss
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    Functioni

    Molecular chaperone that functions in the processing and transport of secreted proteins. When associated with CNPY3, required for proper folding of Toll-like receptors. Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei107 – 1071ATPBy similarity
    Binding sitei149 – 1491ATPBy similarity
    Binding sitei168 – 1681ATPBy similarity
    Binding sitei199 – 1991ATP; via amide nitrogenBy similarity
    Binding sitei448 – 4481ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. low-density lipoprotein particle receptor binding Source: AgBase
    3. RNA binding Source: AgBase
    4. virion binding Source: Ensembl

    GO - Biological processi

    1. actin rod assembly Source: Ensembl
    2. cellular response to ATP Source: Ensembl
    3. ER-associated ubiquitin-dependent protein catabolic process Source: UniProtKB
    4. negative regulation of apoptotic process Source: AgBase
    5. protein folding Source: InterPro
    6. regulation of phosphoprotein phosphatase activity Source: Ensembl
    7. response to hypoxia Source: AgBase

    Keywords - Molecular functioni

    Chaperone

    Keywords - Ligandi

    ATP-binding, Calcium, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_209041. Scavenging by Class A Receptors.
    REACT_224406. ATF6-alpha activates chaperone genes.
    REACT_225126. Trafficking and processing of endosomal TLR.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Endoplasmin
    Alternative name(s):
    94 kDa glucose-regulated protein
    Short name:
    GRP-94
    Heat shock protein 90 kDa beta member 1
    Gene namesi
    Name:HSP90B1
    Synonyms:GRP94, TRA1
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 5

    Subcellular locationi

    Endoplasmic reticulum lumen By similarity. Melanosome By similarity

    GO - Cellular componenti

    1. cytosol Source: AgBase
    2. endoplasmic reticulum Source: AgBase
    3. endoplasmic reticulum lumen Source: UniProtKB-SubCell
    4. endoplasmic reticulum membrane Source: Ensembl
    5. melanosome Source: UniProtKB-SubCell
    6. midbody Source: Ensembl
    7. perinuclear region of cytoplasm Source: AgBase
    8. plasma membrane Source: Ensembl

    Keywords - Cellular componenti

    Endoplasmic reticulum

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2121Sequence AnalysisAdd
    BLAST
    Chaini22 – 804783EndoplasminPRO_0000013596Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi62 – 621N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi107 – 1071N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi138 – 138InterchainBy similarity
    Glycosylationi217 – 2171N-linked (GlcNAc...)Sequence Analysis
    Modified residuei306 – 3061PhosphoserineBy similarity
    Modified residuei404 – 4041N6-succinyllysineBy similarity
    Glycosylationi445 – 4451N-linked (GlcNAc...)Sequence Analysis
    Modified residuei479 – 4791N6-acetyllysineBy similarity
    Glycosylationi481 – 4811N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi502 – 5021N-linked (GlcNAc...)Sequence Analysis
    Modified residuei633 – 6331N6-succinyllysineBy similarity

    Post-translational modificationi

    Phosphorylated.By similarity

    Keywords - PTMi

    Acetylation, Disulfide bond, Glycoprotein, Phosphoprotein

    Proteomic databases

    PaxDbiQ95M18.
    PRIDEiQ95M18.

    Interactioni

    Subunit structurei

    Homodimer; disulfide-linked. Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5 By similarity. Part of a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX. Interacts with AIMP1; regulates its retention in the endoplasmic reticulum. Interacts with OS9 By similarity. Interacts with CNPY3; this interaction is disrupted in the presence of ATP. Interacts with several TLRs, including TLR4 and TLR9, but not with TLR3 By similarity. Interacts with MZB1 in a calcium-dependent manner By similarity. Interacts with METTL23 By similarity.By similarity

    Protein-protein interaction databases

    IntActiQ95M18. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ95M18.
    SMRiQ95M18. Positions 69-755.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi801 – 8044Prevents secretion from ERSequence Analysis

    Sequence similaritiesi

    Belongs to the heat shock protein 90 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG0326.
    GeneTreeiENSGT00750000117672.
    HOGENOMiHOG000031988.
    HOVERGENiHBG007374.
    InParanoidiQ95M18.
    KOiK09487.
    OMAiQSSHHPS.
    OrthoDBiEOG780RM0.
    TreeFamiTF105969.

    Family and domain databases

    Gene3Di3.30.565.10. 2 hits.
    HAMAPiMF_00505. HSP90.
    InterProiIPR003594. HATPase_ATP-bd.
    IPR019805. Heat_shock_protein_90_CS.
    IPR001404. Hsp90_fam.
    IPR020575. Hsp90_N.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    [Graphical view]
    PANTHERiPTHR11528. PTHR11528. 1 hit.
    PfamiPF00183. HSP90. 1 hit.
    [Graphical view]
    PIRSFiPIRSF002583. Hsp90. 1 hit.
    PRINTSiPR00775. HEATSHOCK90.
    SMARTiSM00387. HATPase_c. 1 hit.
    [Graphical view]
    SUPFAMiSSF54211. SSF54211. 1 hit.
    SSF55874. SSF55874. 2 hits.
    PROSITEiPS00014. ER_TARGET. 1 hit.
    PS00298. HSP90. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q95M18-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRALWVLGLC CVLLTFGSVR ADDEVDVDGT VEEDLGKSRE GSRTDDEVVQ    50
    REEEAIQLDG LNASQIRELR EKSEKFAFQA EVNRMMKLII NSLYKNKEIF 100
    LRELISNASD ALDKIRLISL TDENALAGNE ELTVKIKCDK EKNLLHVTDT 150
    GVGMTREELV KNLGTIAKSG TSEFLNKMTE AQEDGQSTSE LIGQFGVGFY 200
    SAFLVADKVI VTSKHNNDTQ HIWESDSNEF SVIADPRGNT LGRGTTITLV 250
    LKEEASDYLE LDTIKNLVKK YSQFINFPIY VWSSKTETVE EPAEEEEAAK 300
    EDKEESDDEA AVEEEEDEKK PKTKKVEKTV WDWELMNDIK PIWQRPSKEV 350
    EEDEYKAFYK SFSKESDDPM AYIHFTAEGE VTFKSILFVP TSAPRGLFDE 400
    YGSKKSDYIK LYVRRVFITD DFHDMMPKYL NFVKGVVDSD DLPLNVSRET 450
    LQQHKLLKVI RKKLVRKTLD MIKKIADEKY NDTFWKEFGT NIKLGVIEDH 500
    SNRTRLAKLL RFQSSHHPSD MTSLDQYVER MKEKQDKIYF MAGASRKEAE 550
    SSPFVERLLK KGYEVIYLTE PVDEYCIQAL PEFDGKRFQN VAKEGVKFDE 600
    SEKSKESREA VEKEFEPLLN WMKDKALKDK IEKAVVSQRL TESPCALVAS 650
    QYGWSGNMER IMKAQAYQTG KDISTNYYAS QKKTFEINPR HPLIRDMLRR 700
    VKEDEDDKTV SDLAVVLFET ATLRSGYLLP DTKAYGDRIE RMLRLSLNID 750
    PDAKVEEEPE EEPEETTEDT AEDTEQDEEE EMDAGTDEEE QETAEKSTAE 800
    KDEL 804
    Length:804
    Mass (Da):92,427
    Last modified:December 1, 2001 - v1
    Checksum:i0A204A77E61F1FDE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB025193 mRNA. Translation: BAB69766.1.
    BC104549 mRNA. Translation: AAI04550.1.
    RefSeqiNP_777125.1. NM_174700.2.
    UniGeneiBt.8686.

    Genome annotation databases

    EnsembliENSBTAT00000004364; ENSBTAP00000004364; ENSBTAG00000003362.
    GeneIDi282646.
    KEGGibta:282646.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB025193 mRNA. Translation: BAB69766.1 .
    BC104549 mRNA. Translation: AAI04550.1 .
    RefSeqi NP_777125.1. NM_174700.2.
    UniGenei Bt.8686.

    3D structure databases

    ProteinModelPortali Q95M18.
    SMRi Q95M18. Positions 69-755.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q95M18. 1 interaction.

    Proteomic databases

    PaxDbi Q95M18.
    PRIDEi Q95M18.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000004364 ; ENSBTAP00000004364 ; ENSBTAG00000003362 .
    GeneIDi 282646.
    KEGGi bta:282646.

    Organism-specific databases

    CTDi 7184.

    Phylogenomic databases

    eggNOGi COG0326.
    GeneTreei ENSGT00750000117672.
    HOGENOMi HOG000031988.
    HOVERGENi HBG007374.
    InParanoidi Q95M18.
    KOi K09487.
    OMAi QSSHHPS.
    OrthoDBi EOG780RM0.
    TreeFami TF105969.

    Enzyme and pathway databases

    Reactomei REACT_209041. Scavenging by Class A Receptors.
    REACT_224406. ATF6-alpha activates chaperone genes.
    REACT_225126. Trafficking and processing of endosomal TLR.

    Miscellaneous databases

    NextBioi 20806327.

    Family and domain databases

    Gene3Di 3.30.565.10. 2 hits.
    HAMAPi MF_00505. HSP90.
    InterProi IPR003594. HATPase_ATP-bd.
    IPR019805. Heat_shock_protein_90_CS.
    IPR001404. Hsp90_fam.
    IPR020575. Hsp90_N.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    [Graphical view ]
    PANTHERi PTHR11528. PTHR11528. 1 hit.
    Pfami PF00183. HSP90. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF002583. Hsp90. 1 hit.
    PRINTSi PR00775. HEATSHOCK90.
    SMARTi SM00387. HATPase_c. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54211. SSF54211. 1 hit.
    SSF55874. SSF55874. 2 hits.
    PROSITEi PS00014. ER_TARGET. 1 hit.
    PS00298. HSP90. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of bovine (Bos taurus) cDNA encoding a 94-kDa glucose-regulated protein and developmental changes in its mRNA and protein content in the mammary gland."
      Watanabe A., Uchida I., Nakata K., Fujimoto Y., Oikawa S.
      Comp. Biochem. Physiol. 130B:547-557(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Mammary gland.
    2. NIH - Mammalian Gene Collection (MGC) project
      Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Hereford.
      Tissue: Uterus.

    Entry informationi

    Entry nameiENPL_BOVIN
    AccessioniPrimary (citable) accession number: Q95M18
    Secondary accession number(s): Q3MHX8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 25, 2003
    Last sequence update: December 1, 2001
    Last modified: October 1, 2014
    This is version 108 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3