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Protein

Acidic mammalian chitinase

Gene

CHIA

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Degrades chitin and chitotriose. May participate in the defense against nematodes, fungi and other pathogens. Plays a role in T-helper cell type 2 (Th2) immune response. Contributes to the response to IL-13 and inflammation in response to IL-13. Stimulates chemokine production by pulmonary epithelial cells. Protects lung epithelial cells against apoptosis and promotes phosphorylation of AKT1. Its function in the inflammatory response and in protecting cells against apoptosis is inhibited by allosamidin, suggesting that the function of this protein depends on carbohydrate binding (By similarity).By similarity1 Publication

Catalytic activityi

Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.

pH dependencei

Optimum pH is 5.6-7.6.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei140 – 1401Proton donorPROSITE-ProRule annotation
Binding sitei141 – 1411ChitooligosaccharideBy similarity
Binding sitei360 – 3601ChitooligosaccharideBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Apoptosis, Carbohydrate metabolism, Chitin degradation, Immunity, Inflammatory response, Polysaccharide degradation

Keywords - Ligandi

Chitin-binding

Protein family/group databases

CAZyiCBM14. Carbohydrate-Binding Module Family 14.
GH18. Glycoside Hydrolase Family 18.

Names & Taxonomyi

Protein namesi
Recommended name:
Acidic mammalian chitinase (EC:3.2.1.14)
Short name:
AMCase
Alternative name(s):
Chitin-binding protein b04
Short name:
CBPb04
Gene namesi
Name:CHIA
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 21211 PublicationAdd
BLAST
Chaini22 – 472451Acidic mammalian chitinasePRO_0000011943Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi26 ↔ 51PROSITE-ProRule annotation
Disulfide bondi49 ↔ 394PROSITE-ProRule annotation
Disulfide bondi307 ↔ 372PROSITE-ProRule annotation
Glycosylationi395 – 3951N-linked (GlcNAc...)Sequence analysis
Glycosylationi409 – 4091N-linked (GlcNAc...)Sequence analysis
Glycosylationi415 – 4151N-linked (GlcNAc...)Sequence analysis
Disulfide bondi456 ↔ 469PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ95M17.
PRIDEiQ95M17.

Expressioni

Tissue specificityi

Detected in liver and in serum.1 Publication

Interactioni

Subunit structurei

Interacts with EGFR.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000000324.

Structurei

3D structure databases

ProteinModelPortaliQ95M17.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini423 – 47250Chitin-binding type-2PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni70 – 712Chitooligosaccharide bindingBy similarity
Regioni97 – 1004Chitooligosaccharide bindingBy similarity
Regioni210 – 2134Chitooligosaccharide bindingBy similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi417 – 4204Poly-Ser

Sequence similaritiesi

Contains 1 chitin-binding type-2 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2806. Eukaryota.
COG3325. LUCA.
HOGENOMiHOG000111109.
HOVERGENiHBG011684.
InParanoidiQ95M17.
KOiK01183.

Family and domain databases

Gene3Di2.170.140.10. 1 hit.
3.10.50.10. 1 hit.
3.20.20.80. 2 hits.
InterProiIPR002557. Chitin-bd_dom.
IPR011583. Chitinase_II.
IPR029070. Chitinase_insertion.
IPR001223. Glyco_hydro18_cat.
IPR001579. Glyco_hydro_18_chit_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF01607. CBM_14. 1 hit.
PF00704. Glyco_hydro_18. 1 hit.
[Graphical view]
SMARTiSM00494. ChtBD2. 1 hit.
SM00636. Glyco_18. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 2 hits.
SSF54556. SSF54556. 1 hit.
SSF57625. SSF57625. 1 hit.
PROSITEiPS50940. CHIT_BIND_II. 1 hit.
PS01095. CHITINASE_18. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q95M17-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKLIFLTGL AFLLNAQLGS AYQLVCYFSN WAQYRPGLGS FKPDNIDPCL
60 70 80 90 100
CTHLIYAFAG MSNSEITTIE WNDVALYSSF NDLKKKNSQL KILLAIGGWN
110 120 130 140 150
FGTAPFTAMV ATPENRKTFI SSVIKFLHQY GFDGLDFDWE YPGFRGSPSQ
160 170 180 190 200
DKHLFTVLVQ ETREAFEQEA KQTNKPRLLV TAAVAAGISN IQAGYEIPQL
210 220 230 240 250
SQYLDFIHVM TYDFHGSWEG YTGENSPLYK YPTDTGSNTY LNVEYAMNYW
260 270 280 290 300
KKNGAPAEKL IIGFPAYGHN FILRDASNNG IGAPTSGAGP AGPYTREAGF
310 320 330 340 350
WAYYEICAFL KDGATEAWDD SQNVPYAYKG TEWVGYDNVN SFRIKAQWLK
360 370 380 390 400
ENNFGGAMVW AIDLDDFTGT FCNQGKFPLI NTLKDALGLK SATCNASTQS
410 420 430 440 450
SEPNSSPGNE SGSGNKSSSS EGRGYCAGKA DGLYPVADNR NAFWNCVNGI
460 470
TYKQNCLTGL VFDTSCHCCN WA
Length:472
Mass (Da):52,129
Last modified:December 1, 2001 - v1
Checksum:i7A4A600E8DA04B1E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti144 – 1441F → S in AAI02932 (Ref. 2) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB051629 mRNA. Translation: BAB71805.1.
BC102931 mRNA. Translation: AAI02932.1.
RefSeqiNP_777124.1. NM_174699.2.
UniGeneiBt.66344.

Genome annotation databases

GeneIDi282645.
KEGGibta:282645.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB051629 mRNA. Translation: BAB71805.1.
BC102931 mRNA. Translation: AAI02932.1.
RefSeqiNP_777124.1. NM_174699.2.
UniGeneiBt.66344.

3D structure databases

ProteinModelPortaliQ95M17.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000000324.

Protein family/group databases

CAZyiCBM14. Carbohydrate-Binding Module Family 14.
GH18. Glycoside Hydrolase Family 18.

Proteomic databases

PaxDbiQ95M17.
PRIDEiQ95M17.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi282645.
KEGGibta:282645.

Organism-specific databases

CTDi27159.

Phylogenomic databases

eggNOGiKOG2806. Eukaryota.
COG3325. LUCA.
HOGENOMiHOG000111109.
HOVERGENiHBG011684.
InParanoidiQ95M17.
KOiK01183.

Family and domain databases

Gene3Di2.170.140.10. 1 hit.
3.10.50.10. 1 hit.
3.20.20.80. 2 hits.
InterProiIPR002557. Chitin-bd_dom.
IPR011583. Chitinase_II.
IPR029070. Chitinase_insertion.
IPR001223. Glyco_hydro18_cat.
IPR001579. Glyco_hydro_18_chit_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF01607. CBM_14. 1 hit.
PF00704. Glyco_hydro_18. 1 hit.
[Graphical view]
SMARTiSM00494. ChtBD2. 1 hit.
SM00636. Glyco_18. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 2 hits.
SSF54556. SSF54556. 1 hit.
SSF57625. SSF57625. 1 hit.
PROSITEiPS50940. CHIT_BIND_II. 1 hit.
PS01095. CHITINASE_18. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A novel serum chitinase that is expressed in bovine liver."
    Suzuki M., Morimatsu M., Yamashita T., Iwanaga T., Syuto B.
    FEBS Lett. 506:127-130(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 22-40; 126-171 AND 312-350, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Tissue: Liver.
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Fetal liver.

Entry informationi

Entry nameiCHIA_BOVIN
AccessioniPrimary (citable) accession number: Q95M17
Secondary accession number(s): Q3SZE1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: December 1, 2001
Last modified: June 8, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.