Reviewed, UniProtKB/Swiss-Prot Q95M17 (CHIA_BOVIN)

Names and origin

Protein names

Recommended name:
    Acidic mammalian chitinase
      Short name=AMCase
    EC=3.2.1.14
Alternative name(s):
    Chitin-binding protein b04
      Short name=CBPb04

Gene names

Name:

CHIA

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	472 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Degrades chitin. May participate in the defense against nematodes and other pathogens. Ref.1
Catalytic activity	Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.
Subcellular location	Secreted. Ref.1
Tissue specificity	Detected in liver and in serum. Ref.1
Sequence similarities	Belongs to the glycosyl hydrolase 18 family. Chitinase class II subfamily. Contains 1 chitin-binding type-2 domain.
Biophysicochemical properties	pH dependence: Optimum pH is 5.6-7.6.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Chitin degradation Polysaccharide degradation
Cellular component	Secreted
Domain	Signal
Ligand	Chitin-binding
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	chitin catabolic process Inferred from electronic annotation. Source: UniProtKB-KW polysaccharide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro chitin binding Inferred from electronic annotation. Source: UniProtKB-KW chitinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Ref.1
	Chain	22 – 472	451	Acidic mammalian chitinase		PRO_0000011943
Regions
	Domain	423 – 472	50	Chitin-binding type-2
	Compositional bias	417 – 420	4	Poly-Ser
Sites
	Active site	140	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	395	1	N-linked (GlcNAc...) Potential
	Glycosylation	409	1	N-linked (GlcNAc...) Potential
	Glycosylation	415	1	N-linked (GlcNAc...) Potential
	Disulfide bond	26 ↔ 51		By similarity
	Disulfide bond	307 ↔ 372		By similarity
	Disulfide bond	456 ↔ 469		By similarity
Experimental info
	Sequence conflict	144	1	F → S in AAI02932. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q95M17-1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 7A4A600E8DA04B1E	FASTA	472	52,129
	10 20 30 40 50 60 MAKLIFLTGL AFLLNAQLGS AYQLVCYFSN WAQYRPGLGS FKPDNIDPCL CTHLIYAFAG 70 80 90 100 110 120 MSNSEITTIE WNDVALYSSF NDLKKKNSQL KILLAIGGWN FGTAPFTAMV ATPENRKTFI 130 140 150 160 170 180 SSVIKFLHQY GFDGLDFDWE YPGFRGSPSQ DKHLFTVLVQ ETREAFEQEA KQTNKPRLLV 190 200 210 220 230 240 TAAVAAGISN IQAGYEIPQL SQYLDFIHVM TYDFHGSWEG YTGENSPLYK YPTDTGSNTY 250 260 270 280 290 300 LNVEYAMNYW KKNGAPAEKL IIGFPAYGHN FILRDASNNG IGAPTSGAGP AGPYTREAGF 310 320 330 340 350 360 WAYYEICAFL KDGATEAWDD SQNVPYAYKG TEWVGYDNVN SFRIKAQWLK ENNFGGAMVW 370 380 390 400 410 420 AIDLDDFTGT FCNQGKFPLI NTLKDALGLK SATCNASTQS SEPNSSPGNE SGSGNKSSSS 430 440 450 460 470 EGRGYCAGKA DGLYPVADNR NAFWNCVNGI TYKQNCLTGL VFDTSCHCCN WA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel serum chitinase that is expressed in bovine liver." Suzuki M., Morimatsu M., Yamashita T., Iwanaga T., Syuto B. FEBS Lett. 506:127-130(2001) [PubMed: 11591385] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 22-40; 126-171 AND 312-350, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY. Tissue: Liver.
[2]	NIH - Mammalian Gene Collection (MGC) project Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Fetal liver.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB051629 mRNA. Translation: BAB71805.1. BC102931 mRNA. Translation: AAI02932.1.
IPI	IPI00704127.
RefSeq	NP_777124.1.
UniGene	Bt.66344
3D structure databases
HSSP	HSSP built from PDB template 1E9L based on UniProtKB O35744.
ModBase	Search...
Protein family/group databases
CAZy	CBM14. Carbohydrate-Binding Module Family 14. GH18. Glycoside Hydrolase Family 18.
Genome annotation databases
Ensembl	ENSBTAG00000000259. Bos taurus. [Contig view]
GeneID	282645.
KEGG	bta:282645.
Phylogenomic databases
HOVERGEN	Q95M17.
Enzyme and pathway databases
BRENDA	3.2.1.14. 251.
Family and domain databases
InterPro	IPR002557. Chitin-bd_peritrophin-A. IPR011583. Chitinase_II. IPR001223. Glyco_hydro18cat. IPR001579. Glyco_hydro_18_chit_AS. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF01607. CBM_14. 1 hit. PF00704. Glyco_hydro_18. 1 hit. [Graphical view]
ProDom	PD000471. Chitinase_II. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00494. ChtBD2. 1 hit. SM00636. Glyco_18. 1 hit. [Graphical view]
PROSITE	PS50940. CHIT_BIND_II. 1 hit. PS01095. CHITINASE_18. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHIA_BOVIN

Accession

Primary (citable) accession number: Q95M17
Secondary accession number(s): Q3SZE1

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	December 1, 2001
Last modified:	June 16, 2009
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)