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Protein

Protein disulfide-isomerase-like protein of the testis

Gene

PDILT

Organism
Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Probable redox-inactive chaperone involved in spermatogenesis.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chaperone, Developmental protein, Isomerase

Keywords - Biological processi

Differentiation, Spermatogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
Protein disulfide-isomerase-like protein of the testis
Gene namesi
Name:PDILT
ORF Names:QtsA-10382, QtsA-15920, QtsA-20110
OrganismiMacaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Taxonomic identifieri9541 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniCercopithecidaeCercopithecinaeMacaca

Subcellular locationi

  • Endoplasmic reticulum PROSITE-ProRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 17Sequence analysisAdd BLAST17
ChainiPRO_000032585018 – 583Protein disulfide-isomerase-like protein of the testisAdd BLAST566

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi58N-linked (GlcNAc...)Sequence analysis1
Glycosylationi128N-linked (GlcNAc...)Sequence analysis1
Glycosylationi160N-linked (GlcNAc...)Sequence analysis1
Glycosylationi340N-linked (GlcNAc...)Sequence analysis1
Glycosylationi540N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

N-glycosylated.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Subunit structurei

Homodimer. The homodimer is not disulfide-linked. Interacts with ERO1A and CLGN (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliQ95LM0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini388 – 451ThioredoxinPROSITE-ProRule annotationAdd BLAST64

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi580 – 583Prevents secretion from ERPROSITE-ProRule annotation4

Domaini

The thioredoxin domain lacks the conserved redox-active Cys at position 417 which is replaced by a Ser residue, suggesting that it lacks thioredoxin activity.

Sequence similaritiesi

Belongs to the protein disulfide isomerase family.Curated
Contains 1 thioredoxin domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG108240.

Family and domain databases

Gene3Di3.40.30.10. 3 hits.
InterProiIPR012336. Thioredoxin-like_fold.
IPR013766. Thioredoxin_domain.
[Graphical view]
PfamiPF00085. Thioredoxin. 2 hits.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 4 hits.
PROSITEiPS00014. ER_TARGET. 1 hit.
PS51352. THIOREDOXIN_2. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q95LM0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDLLWMPLLL VAARISAVHS SPEVNAGVSS IHITKPVHIL EERNLLVLTP
60 70 80 90 100
AGLTQMLNQT RFLMVLFHNP SSKQSRNLAE ELGKAVEIMG KGKNGIGFGK
110 120 130 140 150
VDITVEKELQ QEFGITKAPQ LKLFFEGNRS EPISCKGVVE STALVVWLRR
160 170 180 190 200
QISQKAFLFN SSLQVAEFVT SRPLVIVGFF QDLEEEVAEL FYDVIKDFPE
210 220 230 240 250
LTFGVITIGN AIGRFHVTLD SILVFKKGKI VNRQELINDS TNKQELNRVI
260 270 280 290 300
KQHLTDFVIE YNAENKDLIY ELYIMSHMLL FVSKSSESFG IIIQHYKLAS
310 320 330 340 350
KEFQNKILFI LVNADEPRNR RVIEYFRVTE VDIPSVQILN LSSDARYKMP
360 370 380 390 400
SDDITYENLK KFGRSFLSKN AKKHQSSEEI PKHWDQGLVK QLVGKNFNIV
410 420 430 440 450
VFDKEKDVFV MFYAPWSKKC KMLFPLLEEL GRKYQNHSTI IIAKIDITAN
460 470 480 490 500
DIQLVYLDRY PFFRLFPTDS QQAVLYKGEH TLKGFSDFLE SYIKTSIEDE
510 520 530 540 550
DELLSVEQNE VIEEEVRAKE KEVPMMKKEL PEQQSPELEN VTKHVSKLEE
560 570 580
SAGKKKTSEE VVVVAKPKGP PTQKKKPKVK EEL
Length:583
Mass (Da):66,848
Last modified:December 1, 2001 - v1
Checksum:iCAC54CF9A5F6369C
GO
Isoform 2 (identifier: Q95LM0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     527-553: Missing.

Note: No experimental confirmation available.
Show »
Length:556
Mass (Da):63,831
Checksum:iEE5DD333D60A367E
GO
Isoform 3 (identifier: Q95LM0-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-55: Missing.

Note: No experimental confirmation available.
Show »
Length:528
Mass (Da):60,913
Checksum:i57F2D7BF40991DF3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti375Q → R in BAB64435 (PubMed:12498619).Curated1
Sequence conflicti498E → G in BAB64435 (PubMed:12498619).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0324471 – 55Missing in isoform 3. 1 PublicationAdd BLAST55
Alternative sequenceiVSP_032448527 – 553Missing in isoform 2. 1 PublicationAdd BLAST27

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB071042 mRNA. Translation: BAB64435.1.
AB071063 mRNA. Translation: BAB64456.1.
AB072768 mRNA. Translation: BAB69737.1.
UniGeneiMfa.2579.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB071042 mRNA. Translation: BAB64435.1.
AB071063 mRNA. Translation: BAB64456.1.
AB072768 mRNA. Translation: BAB69737.1.
UniGeneiMfa.2579.

3D structure databases

ProteinModelPortaliQ95LM0.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG108240.

Family and domain databases

Gene3Di3.40.30.10. 3 hits.
InterProiIPR012336. Thioredoxin-like_fold.
IPR013766. Thioredoxin_domain.
[Graphical view]
PfamiPF00085. Thioredoxin. 2 hits.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 4 hits.
PROSITEiPS00014. ER_TARGET. 1 hit.
PS51352. THIOREDOXIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPDILT_MACFA
AccessioniPrimary (citable) accession number: Q95LM0
Secondary accession number(s): Q95LX8, Q95LZ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: December 1, 2001
Last modified: May 11, 2016
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.