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Q95LM0 (PDILT_MACFA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Protein disulfide-isomerase-like protein of the testis
Gene names
Name:PDILT
ORF Names:QtsA-10382, QtsA-15920, QtsA-20110
OrganismMacaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Taxonomic identifier9541 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Cercopithecidae › Cercopithecinae › Macaca

Protein attributes

Sequence length583 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Probable redox-inactive chaperone involved in spermatogenesis By similarity.

Subunit structure

Homodimer. The homodimer is not disulfide-linked. Interacts with ERO1L and CLGN By similarity.

Subcellular location

Endoplasmic reticulum By similarity.

Domain

The thioredoxin domain lacks the conserved redox-active Cys at position 417 which is replaced by a Ser residue, suggesting that it lacks thioredoxin activity.

Post-translational modification

N-glycosylated By similarity.

Sequence similarities

Belongs to the protein disulfide isomerase family.

Contains 1 thioredoxin domain.

Ontologies

Keywords
   Biological processDifferentiation
Spermatogenesis
   Cellular componentEndoplasmic reticulum
   Coding sequence diversityAlternative splicing
   DomainSignal
   Molecular functionChaperone
Developmental protein
Isomerase
   PTMDisulfide bond
Glycoprotein
Gene Ontology (GO)
   Biological_processcell differentiation

Inferred from electronic annotation. Source: UniProtKB-KW

cell redox homeostasis

Inferred from electronic annotation. Source: InterPro

multicellular organismal development

Inferred from electronic annotation. Source: UniProtKB-KW

spermatogenesis

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentendoplasmic reticulum

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionisomerase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q95LM0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q95LM0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     527-553: Missing.
Note: No experimental confirmation available.
Isoform 3 (identifier: Q95LM0-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-55: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1717 Potential
Chain18 – 583566Protein disulfide-isomerase-like protein of the testis
PRO_0000325850

Regions

Domain388 – 45164Thioredoxin
Motif580 – 5834Prevents secretion from ER Potential

Amino acid modifications

Glycosylation581N-linked (GlcNAc...) Potential
Glycosylation1281N-linked (GlcNAc...) Potential
Glycosylation1601N-linked (GlcNAc...) Potential
Glycosylation3401N-linked (GlcNAc...) Potential
Glycosylation5401N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence1 – 5555Missing in isoform 3.
VSP_032447
Alternative sequence527 – 55327Missing in isoform 2.
VSP_032448

Experimental info

Sequence conflict3751Q → R in BAB64435. Ref.2
Sequence conflict4981E → G in BAB64435. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: CAC54CF9A5F6369C

FASTA58366,848
        10         20         30         40         50         60 
MDLLWMPLLL VAARISAVHS SPEVNAGVSS IHITKPVHIL EERNLLVLTP AGLTQMLNQT 

        70         80         90        100        110        120 
RFLMVLFHNP SSKQSRNLAE ELGKAVEIMG KGKNGIGFGK VDITVEKELQ QEFGITKAPQ 

       130        140        150        160        170        180 
LKLFFEGNRS EPISCKGVVE STALVVWLRR QISQKAFLFN SSLQVAEFVT SRPLVIVGFF 

       190        200        210        220        230        240 
QDLEEEVAEL FYDVIKDFPE LTFGVITIGN AIGRFHVTLD SILVFKKGKI VNRQELINDS 

       250        260        270        280        290        300 
TNKQELNRVI KQHLTDFVIE YNAENKDLIY ELYIMSHMLL FVSKSSESFG IIIQHYKLAS 

       310        320        330        340        350        360 
KEFQNKILFI LVNADEPRNR RVIEYFRVTE VDIPSVQILN LSSDARYKMP SDDITYENLK 

       370        380        390        400        410        420 
KFGRSFLSKN AKKHQSSEEI PKHWDQGLVK QLVGKNFNIV VFDKEKDVFV MFYAPWSKKC 

       430        440        450        460        470        480 
KMLFPLLEEL GRKYQNHSTI IIAKIDITAN DIQLVYLDRY PFFRLFPTDS QQAVLYKGEH 

       490        500        510        520        530        540 
TLKGFSDFLE SYIKTSIEDE DELLSVEQNE VIEEEVRAKE KEVPMMKKEL PEQQSPELEN 

       550        560        570        580 
VTKHVSKLEE SAGKKKTSEE VVVVAKPKGP PTQKKKPKVK EEL

« Hide

Isoform 2 [UniParc].

Checksum: EE5DD333D60A367E
Show »

FASTA55663,831
Isoform 3 [UniParc].

Checksum: 57F2D7BF40991DF3
Show »

FASTA52860,913

References

[1]"Isolation of novel full-length cDNA clones from macaque testis cDNA libraries."
Hashimoto K., Osada N., Hida M., Kusuda J., Tanuma R., Hirai M., Terao K., Sugano S.
Submitted (SEP-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
Tissue: Testis.
[2]"Cynomolgus monkey testicular cDNAs for discovery of novel human genes in the human genome sequence."
Osada N., Hida M., Kusuda J., Tanuma R., Hirata M., Suto Y., Hirai M., Terao K., Sugano S., Hashimoto K.
BMC Genomics 3:36-36(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Testis.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AB071042 mRNA. Translation: BAB64435.1.
AB071063 mRNA. Translation: BAB64456.1.
AB072768 mRNA. Translation: BAB69737.1.

3D structure databases

HSSPHSSP built from PDB template 1X5C based on UniProtKB P07237.
ProteinModelPortalQ95LM0.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

HOVERGENHBG108240.
OrthoDBEOG4RFKSG.

Family and domain databases

Gene3D3.40.30.10. 3 hits.
InterProIPR012336. Thioredoxin-like_fold.
IPR013766. Thioredoxin_domain.
[Graphical view]
PfamPF00085. Thioredoxin. 2 hits.
[Graphical view]
SUPFAMSSF52833. Thiordxn-like_fd. 4 hits.
PROSITEPS00014. ER_TARGET. 1 hit.
PS00194. THIOREDOXIN_1. False negative.
PS51352. THIOREDOXIN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePDILT_MACFA
AccessionPrimary (citable) accession number: Q95LM0
Secondary accession number(s): Q95LX8, Q95LZ9
Entry history
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: December 1, 2001
Last modified: April 3, 2013
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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