Reviewed, UniProtKB/Swiss-Prot Q95LG8 (MECP2_MACFA)

Names and origin

Protein names

Recommended name:
    Methyl-CpG-binding protein 2
      Short name=MeCP-2 protein
      Short name=MeCP2

Gene names

Name:

MECP2

Organism

Macaca fascicularis (Crab eating macaque) (Cynomolgus monkey)

Taxonomic identifier

9541 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Cercopithecidae › Cercopithecinae › Macaca

Protein attributes

Sequence length	486 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A.
Subunit structure	Interacts with FNBP3 By similarity. Interacts with CDKL5 By similarity.
Subcellular location	Nucleus By similarity. Note: Colocalized with methyl-CpG in the genome By similarity.
Post-translational modification	Phosphorylated on Ser-423 in brain upon synaptic activity, which attenuates its repressor activity and seems to regulate dendritic growth and spine maturation By similarity.
Sequence similarities	Contains 2 A.T hook DNA-binding domains. Contains 1 MBD (methyl-CpG-binding) domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Repeat
Ligand	DNA-binding
Molecular function	Repressor
PTM	Acetylation Phosphoprotein
Gene Ontology (GO)
Biological process	regulation of transcription Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 486	486	Methyl-CpG-binding protein 2		PRO_0000096346
Regions
	Domain	90 – 162	73	MBD
	DNA binding	185 – 197	13	A.T hook 1
	DNA binding	265 – 277	13	A.T hook 2
	Compositional bias	366 – 372	7	His-rich
	Compositional bias	376 – 405	30	Pro-rich
Amino acid modifications
	Modified residue	80	1	Phosphoserine By similarity
	Modified residue	116	1	Phosphoserine By similarity
	Modified residue	229	1	Phosphoserine By similarity
	Modified residue	423	1	Phosphoserine By similarity
	Modified residue	426	1	Phosphoserine By similarity
	Modified residue	449	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q95LG8-1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 3471B61D90D92A7D	FASTA	486	52,427
	10 20 30 40 50 60 MVAGMLGLRE EKSEDQDLQG LKDKPLKFKK VKKDKKEDKE GKHEPVQPSA HHSAEPAEAG 70 80 90 100 110 120 KAETSEGSGS APAVPEASAS PKQRRSIIRD RGPMYDDPTL PEGWTRKLKQ RKSGRSAGKY 130 140 150 160 170 180 DVYLINPQGK AFRSKVELIA YFEKVGDTSL DPNDFDFTVT GRGSPSRREQ KPPKKPKSPK 190 200 210 220 230 240 APGTGRGRGR PKGSGTTRPK AATSEGVQVK RVLEKSPGKL LVKMPFQTSP GGKAEGGGAT 250 260 270 280 290 300 TSTQVMVIKR PGRKRKAEAD PQAIPKKRGR KPGSVVAAAA AEAKKKAVKE SSIRSVQETV 310 320 330 340 350 360 LPIKKRKTRE TVSIEVKEVV KPLLVSTLGE KSGKGLKTCK SPGRKSKESS PKGRSSSASS 370 380 390 400 410 420 PPKKEHHHHH HHSESPKAPV PLLPPLPPPP PEPESSEDPT SPPEPQDLSS SVCKEEKMPR 430 440 450 460 470 480 GGSLESDGCP KEPAKTQPAV ATAATAAEKY KHRGEGERKD IVSSSMPRPN REEPVDSRTP VTERVS

References

[1]	"Excessive hand-wringing in a MPTP-treated monkey." Muramatsu S. Submitted (AUG-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF295597 mRNA. Translation: AAK97131.1.
3D structure databases
HSSP	HSSP built from PDB template 1QK9 based on UniProtKB P51608.
SMR	Q95LG8. Positions 71-195.
ModBase	Search...
Phylogenomic databases
HOVERGEN	Q95LG8.
Family and domain databases
InterPro	IPR017956. AT_hook_DNA-bd_motif. IPR017353. Methyl_CpG-bd_MeCP2. IPR001739. Methyl_CpG_DNA-bd. [Graphical view]
Gene3D	G3DSA:3.30.890.10. Methyl_CpG_DNA-bd. 1 hit.
Pfam	PF02178. AT_hook. 1 hit. PF01429. MBD. 1 hit. [Graphical view]
PIRSF	PIRSF038006. Methyl_CpG_bd_MeCP2. 1 hit.
SMART	SM00384. AT_hook. 2 hits. SM00391. MBD. 1 hit. [Graphical view]
PROSITE	PS50982. MBD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MECP2_MACFA

Accession

Primary (citable) accession number: Q95LG8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2004
Last sequence update:	December 1, 2001
Last modified:	October 13, 2009
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)