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Reviewed, UniProtKB/Swiss-Prot Q95KV0 (IKKB_BOVIN)

Last modified February 9, 2010. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Inhibitor of nuclear factor kappa-B kinase subunit beta
      Short name=I-kappa-B-kinase beta
      Short name=IkBKB
      Short name=IKK-beta
      Short name=IKK-B
    EC=2.7.11.10
Alternative name(s):
    I-kappa-B kinase 2
      Short name=IKK2
    Nuclear factor NF-kappa-B inhibitor kinase beta
      Short name=NFKBIKB
Gene names
Name: IKBKB
OrganismBos taurus (Bovine)
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

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Protein attributes

Sequence length756 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Function

Acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Also phosphorylates NCOA3 By similarity.

Catalytic activity

ATP + [I-kappa-B protein] = ADP + [I-kappa-B phosphoprotein].

Subunit structure

Component of the I-kappa-B-kinase (IKK) core complex consisting of CHUK, IKBKB and IKBKG; probably four alpha/CHUK-beta/IKBKB dimers are associated with four gamma/IKBKG subunits. The IKK core complex seems to associate with regulatory or adapter proteins to form a IKK-signalosome holo-complex. Part of a complex composed of NCOA2, NCOA3, CHUK/IKKA, IKBKB, IKBKG and CREBBP. Part of a 70-90 kDa complex at least consisting of CHUK/IKKA, IKBKB, NFKBIA, RELA, IKBKAP and MAP3K14. Found in a membrane raft complex, at least composed of BCL10, CARD11, DPP4 and IKBKB. Interacts with SQSTM1 through PRKCZ or PRKCI. Forms an NGF-induced complex with IKBKB, PRKCI and TRAF6. May interact with MAVS/IPS1. Interacts with NALP2. Interacts with TICAM1. Interacts with FAF1; the interaction disrupts the IKK complex formation. Interacts with ATM. Part of a ternary complex consisting of TANK, IKBKB and IKBKG. Interacts with NIBP; the interaction is direct. Interacts with ARRB1 and ARRB2 By similarity.

Subcellular location

Cytoplasm By similarity. Membrane raft By similarity. Note: Colocalized with DPP4 in membrane rafts By similarity.

Post-translational modification

Upon cytokine stimulation, phosphorylated on 'Ser-177' and 'Ser-181' by MEKK1 and/or MAP3K14/NIK; which enhances activity. Once activated, autophosphorylates on the C-terminal serine cluster; which decreases activity and prevents prolonged activation of the inflammatory response By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.

Contains 1 protein kinase domain.

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Ontologies

Keywords
   Cellular componentCytoplasm
Membrane
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Serine/threonine-protein kinase
Transferase
   PTMPhosphoprotein
Gene Ontology (GO)
   Biological processprotein amino acid phosphorylation

Inferred from electronic annotation. Source: InterPro

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

IkappaB kinase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 756756Inhibitor of nuclear factor kappa-B kinase subunit beta
PRO_0000248284

Regions

Domain15 – 300286Protein kinase
Domain458 – 47922Leucine-zipper
Nucleotide binding21 – 299ATP By similarity
Region737 – 7426NEMO-binding By similarity

Sites

Active site1451Proton acceptor By similarity
Binding site441ATP By similarity

Amino acid modifications

Modified residue231Phosphothreonine By similarity
Modified residue1771Phosphoserine By similarity
Modified residue1811Phosphoserine By similarity
Modified residue6701Phosphoserine; by autocatalysis By similarity
Modified residue6721Phosphoserine; by autocatalysis By similarity
Modified residue6751Phosphoserine; by autocatalysis By similarity
Modified residue6821Phosphoserine; by autocatalysis By similarity
Modified residue6891Phosphoserine; by autocatalysis By similarity
Modified residue6971Phosphoserine; by autocatalysis By similarity
Modified residue7051Phosphoserine; by autocatalysis By similarity
Modified residue7331Phosphoserine; by autocatalysis By similarity
Modified residue7401Phosphoserine; by autocatalysis By similarity

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Sequences

Sequence LengthMass (Da)Tools
Q95KV0-1 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: A072D15614A176E5

FASTA75686,647
        10         20         30         40         50         60 
MSWSPSLPTQ TCGAWEMKER LGTGGFGNVI RWHNQETGEQ IAIKQCRQEL SPRNRERWCL 

        70         80         90        100        110        120 
EIQIMRRLNH PNVVAARDVP EGMQSLAPND LPLLAMEYCQ GGDLRKYLNQ FENCCGLREG 

       130        140        150        160        170        180 
AILTLLSDIA SALRYLHENR IIHRDLKPEN IVLQQGEQRL IHKIIDLGYA KELDQGSLCT 

       190        200        210        220        230        240 
SFVGTLQYLA PELLEQQKYT VTVDYWSFGT LAFECITGFR PFLPNWQPVQ WHSKVRQKSE 

       250        260        270        280        290        300 
MDIVVSEDLN GAVKFSSSLP HPNNLNSVLA QRLEKWLQLM LMWHPRQRGT DPVYGPNGCF 

       310        320        330        340        350        360 
KALDDILNLK LLHVLNMVTG TLHTYPVTED ESLQSLKARI RQDTGILEED QELLQEAGLA 

       370        380        390        400        410        420 
LIPDKPAAQC LSDGKLNEGR TLDMDLVFLF DNSRVTYESQ VSPQPQPESV SCILQEPKRN 

       430        440        450        460        470        480 
LPFFQLRKVW GQVWHSIQAL KEDCSRLQQG QRAAMMNLLR NNSCLSKMKN SMASMSQQLK 

       490        500        510        520        530        540 
AKLDFFKTSI QIDLEKYREQ TEFGITSDKL LLAWREMEQA VELCGRENEV KHLVERMMAL 

       550        560        570        580        590        600 
QTDIVDLQRS PMGRKQGGTL DDLEEQAREL YRRLREKPRD QRTDGDSQEM VRLLLQAIQG 

       610        620        630        640        650        660 
FEKKVRVIYT QLSKTVVCKQ KALELLPKVE EVVSLMSEDE KMVVRLQEKR QKELWNLLKI 

       670        680        690        700        710        720 
ACSKVRGPVS GSPDSMNASR LSHPCQLMSQ PCTAPDSLPE AAEKSEDLVA EAHTLCTQLE 

       730        740        750 
NALQDTMKEQ DQSLRSLDWS WLQSEEEEQQ SLERAS

« Hide

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References

« Hide 'large scale' references
[1]"Characterization of the bovine IkappaB kinases (IKK)alpha and IKKbeta, the regulatory subunit NEMO and their substrate IkappaBalpha."
Rottenberg S., Schmuckli-Maurer J., Grimm S., Heussler V.T., Dobbelaere D.A.E.
Gene 299:293-300(2002) [PubMed: 12459277] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Characterization of 954 bovine full-CDS cDNA sequences."
Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L.
BMC Genomics 6:166-166(2005) [PubMed: 16305752] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AJ414556 mRNA. Translation: CAC93687.1.
BT021585 mRNA. Translation: AAX46432.1.
IPIIPI00691106.
RefSeqNP_776778.1.
UniGeneBt.9023

3D structure databases

HSSPHSSP built from PDB template 1MRU based on UniProtKB P0A5S4.
SMRQ95KV0. Positions 11-290, 705-743.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ95KV0.

Genome annotation databases

EnsemblENSBTAT00000009995; ENSBTAP00000009995; ENSBTAG00000007599; Bos taurus. [Genome view]
GeneID281854.
KEGGbta:281854.

Organism-specific databases

CTD281854.

Phylogenomic databases

eggNOGmaNOG04274.
HOVERGENQ95KV0.
PhylomeDBQ95KV0.

Enzyme and pathway databases

BRENDA2.7.11.10. 251.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_cat_dom.
IPR017442. Se/Thr_prot_kinase-like_dom.
IPR008271. Ser/Thr_prot_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
PROSITEPS00107. PROTEIN_KINASE_ATP. False negative.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameIKKB_BOVIN
AccessionPrimary (citable) accession number: Q95KV0
Entry history
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: December 1, 2001
Last modified: February 9, 2010
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents