Reviewed,
UniProtKB/Swiss-Prot Q95KV0 (IKKB_BOVIN)
Last modified
February 9, 2010.
Version 63.
History...
Clusters with 100%,
90%,
50% identity |
Documents (1) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Inhibitor of nuclear factor kappa-B kinase subunit beta Short name=I-kappa-B-kinase beta Short name=IkBKB Short name=IKK-beta Short name=IKK-B EC=2.7.11.10 Alternative name(s): I-kappa-B kinase 2 Short name=IKK2 Nuclear factor NF-kappa-B inhibitor kinase beta Short name=NFKBIKB | ||
| Gene names |
| ||
| Organism | Bos taurus (Bovine) | ||
| Taxonomic identifier | 9913 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Protein attributes
| Sequence length | 756 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at transcript level. |
General annotation (Comments)
| Function | Acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Also phosphorylates NCOA3 By similarity. |
| Catalytic activity | ATP + [I-kappa-B protein] = ADP + [I-kappa-B phosphoprotein]. |
| Subunit structure | Component of the I-kappa-B-kinase (IKK) core complex consisting of CHUK, IKBKB and IKBKG; probably four alpha/CHUK-beta/IKBKB dimers are associated with four gamma/IKBKG subunits. The IKK core complex seems to associate with regulatory or adapter proteins to form a IKK-signalosome holo-complex. Part of a complex composed of NCOA2, NCOA3, CHUK/IKKA, IKBKB, IKBKG and CREBBP. Part of a 70-90 kDa complex at least consisting of CHUK/IKKA, IKBKB, NFKBIA, RELA, IKBKAP and MAP3K14. Found in a membrane raft complex, at least composed of BCL10, CARD11, DPP4 and IKBKB. Interacts with SQSTM1 through PRKCZ or PRKCI. Forms an NGF-induced complex with IKBKB, PRKCI and TRAF6. May interact with MAVS/IPS1. Interacts with NALP2. Interacts with TICAM1. Interacts with FAF1; the interaction disrupts the IKK complex formation. Interacts with ATM. Part of a ternary complex consisting of TANK, IKBKB and IKBKG. Interacts with NIBP; the interaction is direct. Interacts with ARRB1 and ARRB2 By similarity. |
| Subcellular location | Cytoplasm By similarity. Membrane raft By similarity. Note: Colocalized with DPP4 in membrane rafts By similarity. |
| Post-translational modification | Upon cytokine stimulation, phosphorylated on 'Ser-177' and 'Ser-181' by MEKK1 and/or MAP3K14/NIK; which enhances activity. Once activated, autophosphorylates on the C-terminal serine cluster; which decreases activity and prevents prolonged activation of the inflammatory response By similarity. |
| Sequence similarities | Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily. Contains 1 protein kinase domain. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Cytoplasm Membrane |
| Ligand | ATP-binding Nucleotide-binding |
| Molecular function | Kinase Serine/threonine-protein kinase Transferase |
| PTM | Phosphoprotein |
| Gene Ontology (GO) | |
| Biological process | protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro |
| Cellular component | cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | ATP binding Inferred from electronic annotation. Source: UniProtKB-KW IkappaB kinase activityInferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 756 | 756 | Inhibitor of nuclear factor kappa-B kinase subunit beta | PRO_0000248284 | |||||
Regions | |||||||||
| Domain | 15 – 300 | 286 | Protein kinase | ||||||
| Domain | 458 – 479 | 22 | Leucine-zipper | ||||||
| Nucleotide binding | 21 – 29 | 9 | ATP By similarity | ||||||
| Region | 737 – 742 | 6 | NEMO-binding By similarity | ||||||
Sites | |||||||||
| Active site | 145 | 1 | Proton acceptor By similarity | ||||||
| Binding site | 44 | 1 | ATP By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 23 | 1 | Phosphothreonine By similarity | ||||||
| Modified residue | 177 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 181 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 670 | 1 | Phosphoserine; by autocatalysis By similarity | ||||||
| Modified residue | 672 | 1 | Phosphoserine; by autocatalysis By similarity | ||||||
| Modified residue | 675 | 1 | Phosphoserine; by autocatalysis By similarity | ||||||
| Modified residue | 682 | 1 | Phosphoserine; by autocatalysis By similarity | ||||||
| Modified residue | 689 | 1 | Phosphoserine; by autocatalysis By similarity | ||||||
| Modified residue | 697 | 1 | Phosphoserine; by autocatalysis By similarity | ||||||
| Modified residue | 705 | 1 | Phosphoserine; by autocatalysis By similarity | ||||||
| Modified residue | 733 | 1 | Phosphoserine; by autocatalysis By similarity | ||||||
| Modified residue | 740 | 1 | Phosphoserine; by autocatalysis By similarity | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Characterization of the bovine IkappaB kinases (IKK)alpha and IKKbeta, the regulatory subunit NEMO and their substrate IkappaBalpha." Rottenberg S., Schmuckli-Maurer J., Grimm S., Heussler V.T., Dobbelaere D.A.E. Gene 299:293-300(2002) [PubMed: 12459277] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [2] | "Characterization of 954 bovine full-CDS cDNA sequences." Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L. BMC Genomics 6:166-166(2005) [PubMed: 16305752] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AJ414556 mRNA. Translation: CAC93687.1. BT021585 mRNA. Translation: AAX46432.1. |
| IPI | IPI00691106. |
| RefSeq | NP_776778.1. |
| UniGene | Bt.9023 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1MRU based on UniProtKB P0A5S4. |
| SMR | Q95KV0. Positions 11-290, 705-743. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | Q95KV0. |
Genome annotation databases | |
| Ensembl | ENSBTAT00000009995; ENSBTAP00000009995; ENSBTAG00000007599; Bos taurus. [Genome view] |
| GeneID | 281854. |
| KEGG | bta:281854. |
Organism-specific databases | |
| CTD | 281854. |
Phylogenomic databases | |
| eggNOG | maNOG04274. |
| HOVERGEN | Q95KV0. |
| PhylomeDB | Q95KV0. |
Enzyme and pathway databases | |
| BRENDA | 2.7.11.10. 251. |
Family and domain databases | |
| InterPro | IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017442. Se/Thr_prot_kinase-like_dom. IPR008271. Ser/Thr_prot_kinase_AS. [Graphical view] |
| Pfam | PF00069. Pkinase. 1 hit. [Graphical view] |
| PROSITE | PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | IKKB_BOVIN | ||||||||
| Accession | Primary (citable) accession number: Q95KV0 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||

Clusters with


