Reviewed, UniProtKB/Swiss-Prot Q95KC9 (PCYOX_MACFA)

Names and origin

Protein names

Recommended name:
    Prenylcysteine oxidase
    EC=1.8.3.5

Gene names

Name:	PCYOX1
ORF Names:	QmoA-10162

Organism

Macaca fascicularis (Crab eating macaque) (Cynomolgus monkey)

Taxonomic identifier

9541 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Cercopithecidae › Cercopithecinae › Macaca

Protein attributes

Sequence length	505 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Involved in the degradation of prenylated proteins. Cleaves the thioether bond of prenyl-L-cysteines, such as farnesylcysteine and geranylgeranylcysteine By similarity.
Catalytic activity	An S-prenyl-L-cysteine + O2 + H2O = a prenal + L-cysteine + H2O2.
Cofactor	FAD By similarity.
Subcellular location	Lysosome By similarity.
Sequence similarities	Belongs to the prenylcysteine oxidase family.

Ontologies

Keywords
Cellular component	Lysosome
Domain	Signal
Ligand	FAD
Molecular function	Oxidoreductase
PTM	Glycoprotein Isopeptide bond Ubl conjugation
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW prenylcysteine catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	lysosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	prenylcysteine oxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27	Potential
	Chain	28 – 505	478	Prenylcysteine oxidase		PRO_0000023299
Amino acid modifications
	Glycosylation	196	1	N-linked (GlcNAc...) Potential
	Glycosylation	323	1	N-linked (GlcNAc...) Potential
	Glycosylation	353	1	N-linked (GlcNAc...) Potential
	Cross-link	162		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q95KC9-1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 9CC9A113FA132897	FASTA	505	56,517
	10 20 30 40 50 60 MGRAVAELVS SLLGLWLLLC SCGCPEGAEL RAPPDKIAVI GAGIGGTSAA YYLRQKFGKD 70 80 90 100 110 120 VKIDLFEREE VGGRLATMMV QGQEYEAGGS VIHPLNLHMK RFVKDLGLST VQASGGLLGI 130 140 150 160 170 180 YNGEALVFEE SNWFIINVIK LVWRYGFQSL RMHMWVEDVL DKFMRIYRYQ SHDYAFSSVE 190 200 210 220 230 240 KLLHALGGDD FLGMLNRTLL ETLQKAGFSE KFLNEMIAPV MRVDYGQSTD INAFVGAVSL 250 260 270 280 290 300 SCSDSGLWAV EGGNKLVCSG LLQASKSNLI SGSVMYIEEK TKTKHTGNPT KMYEVVYQIG 310 320 330 340 350 360 TETHSDFYDI VLVATPLNRK MSNITFLNFD PPIEEFHQYY QHIVTTLVKG ELNTSIFSSR 370 380 390 400 410 420 PIDKFGLSTV LTTDNSDLFI NSIGIVSSVR EKEDPEPSTD GTYVWKIFSQ ETLTKAQILK 430 440 450 460 470 480 LFLSYDYAVK KPWLAYPHYK PPEKCPSIIL HDRLYYLNGI ECAASAMEMS AIAAHNAALL 490 500 AYHRWNGHTD MIDQDGLYEK LKTEL

References

[1]

"Isolation of full-length cDNA clones from macaque brain cDNA libraries." Osada N., Hida M., Kusuda J., Tanuma R., Iseki K., Hirai M., Terao K., Suzuki Y., Sugano S., Hashimoto K. Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Medulla oblongata.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB062961 mRNA. Translation: BAB60740.1.
3D structure databases
ModBase	Search...
Phylogenomic databases
HOVERGEN	Q95KC9.
Enzyme and pathway databases
BRENDA	1.8.3.5. 3438.
Family and domain databases
InterPro	IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR010795. Prenylcys_lyase. IPR017046. Prenylcysteine_Oxase. [Graphical view]
Pfam	PF07156. Prenylcys_lyase. 1 hit. PF07992. Pyr_redox_2. 1 hit. [Graphical view]
PIRSF	PIRSF036292. Prenylcysteine_oxidase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PCYOX_MACFA

Accession

Primary (citable) accession number: Q95KC9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2005
Last sequence update:	December 1, 2001
Last modified:	June 16, 2009
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)