Q95K78 (NEUM_MACFA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Neuromodulin

Alternative name(s):
Axonal membrane protein GAP-43
Growth-associated protein 43

Gene names

Name:	GAP43
ORF Names:	QtrA-11580, QtrA-13071

Organism

Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)

Taxonomic identifier

9541 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Cercopithecoidea › Cercopithecidae › Cercopithecinae › Macaca

Protein attributes

Sequence length	238 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	This protein is associated with nerve growth. It is a major component of the motile "growth cones" that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction By similarity.
Subunit structure	Identified in a complex containing FGFR4, NCAM1, CDH2, PLCG1, FRS2, SRC, SHC1, GAP43 and CTTN By similarity. Binds calmodulin with a greater affinity in the absence of Ca2+ than in its presence By similarity.
Subcellular location	Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection › growth cone membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Cell junction › synapse By similarity. Cell projection › filopodium membrane; Peripheral membrane protein By similarity. Note: Cytoplasmic surface of growth cone and synaptic plasma membranes By similarity.
Post-translational modification	Palmitoylation by ARF6 is essential for plasma membrane association and axonal and dendritic filopodia induction. Deacylated by LYPLA2 By similarity. Phosphorylated at Ser-41 by PHK. Phosphorylation of this protein by a protein kinase C is specifically correlated with certain forms of synaptic plasticity By similarity.
Sequence similarities	Belongs to the neuromodulin family. Contains 1 IQ domain.

Ontologies

Keywords
Biological process	Differentiation Growth regulation Neurogenesis
Cellular component	Cell junction Cell membrane Cell projection Membrane Synapse
Ligand	Calmodulin-binding
Molecular function	Developmental protein
PTM	Lipoprotein Palmitate Phosphoprotein
Gene Ontology (GO)
Biological_process	cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW nervous system development Inferred from electronic annotation. Source: UniProtKB-KW regulation of growth Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cell junction Inferred from electronic annotation. Source: UniProtKB-KW filopodium membrane Inferred from electronic annotation. Source: UniProtKB-SubCell growth cone membrane Inferred from electronic annotation. Source: UniProtKB-SubCell synapse Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 238	238	Neuromodulin		PRO_0000250206
Regions
	Domain	31 – 60	30	IQ
	Region	1 – 4	4	Important for membrane binding By similarity
Amino acid modifications
	Modified residue	41	1	Phosphoserine; by PHK and PKC By similarity
	Modified residue	94	1	Phosphothreonine By similarity
	Modified residue	181	1	Phosphothreonine By similarity
	Modified residue	202	1	Phosphoserine; by CK2 By similarity
	Modified residue	203	1	Phosphoserine; by CK2 By similarity
	Lipidation	3	1	S-palmitoyl cysteine By similarity
	Lipidation	4	1	S-palmitoyl cysteine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q95K78 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 18BCA051E2519483	FASTA	238	24,789
	10 20 30 40 50 60 MLCCMRRTKQ VEKNDEDQKI EQDGIKPEDK AHKAATKIQA SFRGHITRKK LKGEKKDDAQ 70 80 90 100 110 120 AAEAEANKKD EAPVADGVEK KGEGTTATEA APATGSKPDE PGKAGETPSE EKKGEGDAAT 130 140 150 160 170 180 EQAAPQAPAS SEEKAGSAET ESATKASTDN SPSSKAEDAP AKEEPKQADV PAAVTAAAAT 190 200 210 220 230 TPAAEDAAAK ATAQPPTETG ESSQAEENIE AVDETKPKES ARQDEGKEEE PEADQEHA

References

[1]	"DNA sequences of macaque genes expressed in brain or testis and its evolutionary implications." International consortium for macaque cDNA sequencing and analysis Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Temporal cortex.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB063093 mRNA. Translation: BAB60799.1. AB169784 mRNA. Translation: BAE01865.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG006468.
Family and domain databases
InterPro	IPR000048. IQ_motif_EF-hand-BS. IPR001422. Neuromodulin. IPR017454. Neuromodulin_C. IPR018947. Neuromodulin_gap-junction_N. IPR018243. Neuromodulin_palmitoyl/P_site. [Graphical view]
Pfam	PF00612. IQ. 1 hit. PF06614. Neuromodulin. 1 hit. PF10580. Neuromodulin_N. 1 hit. [Graphical view]
PRINTS	PR00215. NEUROMODULIN.
SMART	SM00015. IQ. 1 hit. [Graphical view]
PROSITE	PS50096. IQ. 1 hit. PS00412. NEUROMODULIN_1. 1 hit. PS00413. NEUROMODULIN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NEUM_MACFA

Accession

Primary (citable) accession number: Q95K78

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2006
Last sequence update:	December 1, 2001
Last modified:	March 6, 2013
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program