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Protein

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1

Gene

PLCZ1

Organism
Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. In vitro, hydrolyzes PtdIns(4,5)P2 in a Ca2+-dependent manner. Triggers intracellular Ca2+ oscillations in oocytes solely during M phase and is involved in inducing oocyte activation and initiating embryonic development up to the blastocyst stage. Is therefore a strong candidate for the egg-activating soluble sperm factor that is transferred from the sperm into the egg cytoplasm following gamete membrane fusion. May exert an inhibitory effect on phospholipase-C-coupled processes that depend on calcium ions and protein kinase C, including CFTR trafficking and function.By similarityCurated1 Publication

Catalytic activityi

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.By similarity

Cofactori

Ca2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei170PROSITE-ProRule annotationBy similarity1
Active sitei215PROSITE-ProRule annotationBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein, Hydrolase, Transducer
Biological processFertilization, Lipid degradation, Lipid metabolism
LigandCalcium

Names & Taxonomyi

Protein namesi
Recommended name:
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 (EC:3.1.4.11)
Alternative name(s):
Phosphoinositide phospholipase C-zeta-1
Phospholipase C-zeta-1By similarity
Short name:
PLC-zeta-1By similarity
Gene namesi
Name:PLCZ1Imported
ORF Names:QtsA-14035, QtsA-14094
OrganismiMacaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Taxonomic identifieri9541 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniCercopithecidaeCercopithecinaeMacaca

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003472451 – 6411-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1Add BLAST641

Interactioni

Subunit structurei

Interacts via its C2 domain with PtdIns3P and, to a lesser extent, PtdIns5P in vitro.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ95JS1
SMRiQ95JS1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini35 – 70EF-handPROSITE-ProRule annotationAdd BLAST36
Domaini155 – 299PI-PLC X-boxPROSITE-ProRule annotationAdd BLAST145
Domaini382 – 498PI-PLC Y-boxPROSITE-ProRule annotationAdd BLAST117
Domaini502 – 605C2PROSITE-ProRule annotationAdd BLAST104

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi320 – 356Glu-richSequence analysisAdd BLAST37

Domaini

The EF-hand and C2 domains are essential for triggering Ca2+ oscillating activity and the regulation of PLCZ1 enzyme activity.By similarity
The X-Y linker region between PI-PLC X-box and Y-box domains may be a target for proteolysis and may play an important regulatory role during fertilization.By similarity

Phylogenomic databases

HOVERGENiHBG053610
KOiK05861

Family and domain databases

Gene3Di2.60.40.1501 hit
InterProiView protein in InterPro
IPR000008 C2_dom
IPR035892 C2_domain_sf
IPR011992 EF-hand-dom_pair
IPR002048 EF_hand_dom
IPR001192 PI-PLC_fam
IPR017946 PLC-like_Pdiesterase_TIM-brl
IPR028395 PLC-zeta1
IPR015359 PLC_EF-hand-like
IPR000909 PLipase_C_PInositol-sp_X_dom
IPR001711 PLipase_C_Pinositol-sp_Y
PANTHERiPTHR10336 PTHR10336, 1 hit
PTHR10336:SF29 PTHR10336:SF29, 1 hit
PfamiView protein in Pfam
PF00168 C2, 1 hit
PF09279 EF-hand_like, 1 hit
PF00388 PI-PLC-X, 1 hit
PF00387 PI-PLC-Y, 1 hit
PRINTSiPR00390 PHPHLIPASEC
SMARTiView protein in SMART
SM00239 C2, 1 hit
SM00148 PLCXc, 1 hit
SM00149 PLCYc, 1 hit
SUPFAMiSSF47473 SSF47473, 1 hit
SSF51695 SSF51695, 1 hit
PROSITEiView protein in PROSITE
PS50004 C2, 1 hit
PS50222 EF_HAND_2, 1 hit
PS50007 PIPLC_X_DOMAIN, 1 hit
PS50008 PIPLC_Y_DOMAIN, 1 hit

Sequencei

Sequence statusi: Complete.

Q95JS1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEMKWFLSKI QDDFRGGKIN LEKTQRLLEK LDIRCSYIHV KRIFKDNDRL
60 70 80 90 100
KQGRITIEEF RAIYRILTHR EEIVEIFNAY SENRKILLEN NLVQFLTQEQ
110 120 130 140 150
YTTEMSKTIA FEIIQKYEPI EEVRKARQMS LEGFTRYMDS RECQLFKNEC
160 170 180 190 200
RKVYQDMTHP LNDYFISSSH NTYLVSDQLV GPSDLWGYVS ALVKGCRCLE
210 220 230 240 250
IDCWDGAQNE PVVYHGYTLT SKLLFKTVIQ AIHKYAFMTS DYPVVLSLEN
260 270 280 290 300
HCSPAQQEIM ADNLQTTFGE SLLSDMLADF PDTLPSPEAL KFKVLVKNKK
310 320 330 340 350
IGTLKETHER KGSDKRGKVE EWEEEVADLE EEEEEEEKFK ESEIFESVLG
360 370 380 390 400
ENQDKETGVK KLSGVTLFKK KKTRKLKIAL ALSDLVIYTK AEKFKSFQHS
410 420 430 440 450
RLYQQFNENN SIGETQARKL SKLRAHEFIF HTRKFITRIY PKATRADSSN
460 470 480 490 500
FNPQEFWNIG CQMVALNFQT PGLPMDLQNG KFLDNGGSGY ILKPHFLRES
510 520 530 540 550
ESYFNPSDIK DSMPITLTIR LISGIQLPLT HSSSNKGDTL VIIEVFGVPN
560 570 580 590 600
DQMKQQTRVI KKNAFSPRWN ETFTFIIHVP ELALIRFVVE SQGLIAGNEF
610 620 630 640
LGQYTLPLLC MNKGYRRVPL FSRMGESLEP ASLFVYVWYV R
Length:641
Mass (Da):74,551
Last modified:December 1, 2001 - v1
Checksum:i2C54E37BDD3C72E4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti337 – 338EK → R in BAB63054 (PubMed:12498619).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB070109 mRNA Translation: BAB63054.1
AB070108 mRNA Translation: BAB63053.1
RefSeqiNP_001306483.1, NM_001319554.1
UniGeneiMfa.2497

Genome annotation databases

GeneIDi102122753
KEGGimcf:102122753

Similar proteinsi

Entry informationi

Entry nameiPLCZ1_MACFA
AccessioniPrimary (citable) accession number: Q95JS1
Secondary accession number(s): Q95JS0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: December 1, 2001
Last modified: March 28, 2018
This is version 88 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program