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Q95JS1 (PLCZ1_MACFA) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1

EC=3.1.4.11
Alternative name(s):
Phosphoinositide phospholipase C-zeta-1
Phospholipase C-zeta-1
Short name=PLC-zeta-1
Gene names
Name:PLCZ1
ORF Names:QtsA-14035, QtsA-14094
OrganismMacaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Taxonomic identifier9541 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniCercopithecidaeCercopithecinaeMacaca

Protein attributes

Sequence length641 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. In vitro, hydrolyzes PtdIns(4,5)P2 in a Ca2+-dependent manner. Triggers intracellular Ca2+ oscillations in oocytes solely during M phase and is involved in inducing oocyte activation and initiating embryonic development up to the blastocyst stage. Is therefore a strong candidate for the egg-activating soluble sperm factor that is transferred from the sperm into the egg cytoplasm following gamete membrane fusion. May exert an inhibitory effect on phospholipase-C-coupled processes that depend on calcium ions and protein kinase C, including CFTR trafficking and function. Ref.1 UniProtKB Q8K4D7 UniProtKB Q86YW0

Catalytic activity

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol. UniProtKB P10688

Cofactor

Calcium By similarity. UniProtKB Q8K4D7

Subunit structure

Interacts via its C2 domain with PtdIns3P and, to a lesser extent, PtdIns5P in vitro By similarity. UniProtKB Q8K4D7

Subcellular location

Nucleus By similarity. Cytoplasmperinuclear region By similarity. Note: Exhibits alternative cytoplasmic/nuclear localization during development. Translocates from the pronucleus into cytoplasm upon nuclear envelope breakdown for mitosis and localizes again to the pronucleus at interphase following meiosis and mitosis By similarity. UniProtKB Q8K4D7

Domain

The EF-hand and C2 domains are essential for triggering Ca2+ oscillating activity and the regulation of PLCZ1 enzyme activity By similarity. UniProtKB Q8K4D7

The X-Y linker region between PI-PLC X-box and Y-box domains may be a target for proteolysis and may play an important regulatory role during fertilization By similarity. UniProtKB Q8K4D7

Sequence similarities

Contains 1 C2 domain.

Contains 1 EF-hand domain.

Contains 1 PI-PLC X-box domain.

Contains 1 PI-PLC Y-box domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 6416411-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1
PRO_0000347245

Regions

Domain35 – 7036EF-hand
Domain155 – 299145PI-PLC X-box
Domain382 – 498117PI-PLC Y-box
Domain502 – 605104C2
Compositional bias320 – 35637Glu-rich

Sites

Active site1701 By similarity UniProtKB P10688
Active site2151 By similarity UniProtKB P10688

Experimental info

Sequence conflict337 – 3382EK → R in BAB63054. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q95JS1 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: 2C54E37BDD3C72E4

FASTA64174,551
        10         20         30         40         50         60 
MEMKWFLSKI QDDFRGGKIN LEKTQRLLEK LDIRCSYIHV KRIFKDNDRL KQGRITIEEF 

        70         80         90        100        110        120 
RAIYRILTHR EEIVEIFNAY SENRKILLEN NLVQFLTQEQ YTTEMSKTIA FEIIQKYEPI 

       130        140        150        160        170        180 
EEVRKARQMS LEGFTRYMDS RECQLFKNEC RKVYQDMTHP LNDYFISSSH NTYLVSDQLV 

       190        200        210        220        230        240 
GPSDLWGYVS ALVKGCRCLE IDCWDGAQNE PVVYHGYTLT SKLLFKTVIQ AIHKYAFMTS 

       250        260        270        280        290        300 
DYPVVLSLEN HCSPAQQEIM ADNLQTTFGE SLLSDMLADF PDTLPSPEAL KFKVLVKNKK 

       310        320        330        340        350        360 
IGTLKETHER KGSDKRGKVE EWEEEVADLE EEEEEEEKFK ESEIFESVLG ENQDKETGVK 

       370        380        390        400        410        420 
KLSGVTLFKK KKTRKLKIAL ALSDLVIYTK AEKFKSFQHS RLYQQFNENN SIGETQARKL 

       430        440        450        460        470        480 
SKLRAHEFIF HTRKFITRIY PKATRADSSN FNPQEFWNIG CQMVALNFQT PGLPMDLQNG 

       490        500        510        520        530        540 
KFLDNGGSGY ILKPHFLRES ESYFNPSDIK DSMPITLTIR LISGIQLPLT HSSSNKGDTL 

       550        560        570        580        590        600 
VIIEVFGVPN DQMKQQTRVI KKNAFSPRWN ETFTFIIHVP ELALIRFVVE SQGLIAGNEF 

       610        620        630        640 
LGQYTLPLLC MNKGYRRVPL FSRMGESLEP ASLFVYVWYV R 

« Hide

References

« Hide 'large scale' references
[1]"Sperm phospholipase Czeta from humans and cynomolgus monkeys triggers Ca2+ oscillations, activation and development of mouse oocytes."
Cox L.J., Larman M.G., Saunders C.M., Hashimoto K., Swann K., Lai F.A.
Reproduction 124:611-623(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
Tissue: Testis.
[2]"Cynomolgus monkey testicular cDNAs for discovery of novel human genes in the human genome sequence."
Osada N., Hida M., Kusuda J., Tanuma R., Hirata M., Suto Y., Hirai M., Terao K., Sugano S., Hashimoto K.
BMC Genomics 3:36-36(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Testis.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB070109 mRNA. Translation: BAB63054.1.
AB070108 mRNA. Translation: BAB63053.1.

3D structure databases

ProteinModelPortalQ95JS1.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

HOVERGENHBG053610.

Family and domain databases

Gene3D1.10.238.10. 2 hits.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProIPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR002048. EF_hand_dom.
IPR001192. PI-PLC_fam.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR028395. PLC-zeta1.
IPR015359. PLipase_C_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view]
PANTHERPTHR10336. PTHR10336. 1 hit.
PTHR10336:SF29. PTHR10336:SF29. 1 hit.
PfamPF00168. C2. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
[Graphical view]
PRINTSPR00390. PHPHLIPASEC.
SMARTSM00239. C2. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view]
SUPFAMSSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEPS50004. C2. 1 hit.
PS50222. EF_HAND_2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePLCZ1_MACFA
AccessionPrimary (citable) accession number: Q95JS1
Secondary accession number(s): Q95JS0
Entry history
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: December 1, 2001
Last modified: July 9, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families