Reviewed, UniProtKB/Swiss-Prot Q95JS1 (PLCZ1_MACFA)

Names and origin

Protein names

Recommended name:
    1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase zeta-1
    EC=3.1.4.11
Alternative name(s):
    Phospholipase C-zeta-1
      Short name=PLC-zeta-1

Gene names

Name:	PLCZ1
ORF Names:	QtsA-14035, QtsA-14094

Organism

Macaca fascicularis (Crab eating macaque) (Cynomolgus monkey)

Taxonomic identifier

9541 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Cercopithecidae › Cercopithecinae › Macaca

Protein attributes

Sequence length	641 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. In vitro, hydrolyzes PtdIns(4,5)P2 in a Ca2+-dependent manner. Triggers intracellular Ca2+ oscillations in oocytes solely during M phase and is involved in inducing oocyte activation and initiating embryonic development up to the blastocyst stage. Is therefore a strong candidate for the egg-activating soluble sperm factor that is transferred from the sperm into the egg cytoplasm following gamete membrane fusion. May exert an inhibitory effect on phospholipase-C-coupled processes that depend on calcium ions and protein kinase C, including CFTR trafficking and function. Ref.1 UniProtKB Q8K4D7 UniProtKB Q86YW0
Catalytic activity	1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol. UniProtKB P10688
Cofactor	Calcium By similarity. UniProtKB Q8K4D7
Subunit structure	Interacts via its C2 domain with PtdIns3P and, to a lesser extent, PtdIns5P in vitro By similarity. UniProtKB Q8K4D7
Subcellular location	Nucleus By similarity. Cytoplasm › perinuclear region By similarity. Note: Exhibits alternative cytoplasmic/nuclear localization during development. Translocates from the pronucleus into cytoplasm upon nuclear envelope breakdown for mitosis and localizes again to the pronucleus at interphase following meiosis and mitosis By similarity. UniProtKB Q8K4D7
Domain	The EF-hand and C2 domains are essential for triggering Ca2+ oscillating activity and the regulation of PLCZ1 enzyme activity By similarity. UniProtKB Q8K4D7 The X-Y linker region between PI-PLC X-box and Y-box domains may be a target for proteolysis and may play an important regulatory role during fertilization By similarity. UniProtKB Q8K4D7
Sequence similarities	Contains 1 C2 domain. Contains 1 EF-hand domain. Contains 1 PI-PLC X-box domain. Contains 1 PI-PLC Y-box domain.

Ontologies

Keywords
Biological process	Fertilization Lipid degradation
Cellular component	Cytoplasm Nucleus
Ligand	Calcium
Molecular function	Developmental protein Hydrolase Transducer
Gene Ontology (GO)
Biological process	intracellular signaling cascade Inferred from electronic annotation. Source: InterPro lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW single fertilization Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell perinuclear region of cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoinositide phospholipase C activity Inferred from electronic annotation. Source: EC signal transducer activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 641	641	1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase zeta-1		PRO_0000347245
Regions
	Domain	35 – 70	36	EF-hand
	Domain	155 – 299	145	PI-PLC X-box
	Domain	382 – 498	117	PI-PLC Y-box
	Domain	502 – 605	104	C2
	Compositional bias	320 – 356	37	Glu-rich
Sites
	Active site	170	1	By similarity UniProtKB P10688
	Active site	215	1	By similarity UniProtKB P10688
Experimental info
	Sequence conflict	337 – 338	2	EK → R in BAB63054. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q95JS1-1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 2C54E37BDD3C72E4	FASTA	641	74,551
	10 20 30 40 50 60 MEMKWFLSKI QDDFRGGKIN LEKTQRLLEK LDIRCSYIHV KRIFKDNDRL KQGRITIEEF 70 80 90 100 110 120 RAIYRILTHR EEIVEIFNAY SENRKILLEN NLVQFLTQEQ YTTEMSKTIA FEIIQKYEPI 130 140 150 160 170 180 EEVRKARQMS LEGFTRYMDS RECQLFKNEC RKVYQDMTHP LNDYFISSSH NTYLVSDQLV 190 200 210 220 230 240 GPSDLWGYVS ALVKGCRCLE IDCWDGAQNE PVVYHGYTLT SKLLFKTVIQ AIHKYAFMTS 250 260 270 280 290 300 DYPVVLSLEN HCSPAQQEIM ADNLQTTFGE SLLSDMLADF PDTLPSPEAL KFKVLVKNKK 310 320 330 340 350 360 IGTLKETHER KGSDKRGKVE EWEEEVADLE EEEEEEEKFK ESEIFESVLG ENQDKETGVK 370 380 390 400 410 420 KLSGVTLFKK KKTRKLKIAL ALSDLVIYTK AEKFKSFQHS RLYQQFNENN SIGETQARKL 430 440 450 460 470 480 SKLRAHEFIF HTRKFITRIY PKATRADSSN FNPQEFWNIG CQMVALNFQT PGLPMDLQNG 490 500 510 520 530 540 KFLDNGGSGY ILKPHFLRES ESYFNPSDIK DSMPITLTIR LISGIQLPLT HSSSNKGDTL 550 560 570 580 590 600 VIIEVFGVPN DQMKQQTRVI KKNAFSPRWN ETFTFIIHVP ELALIRFVVE SQGLIAGNEF 610 620 630 640 LGQYTLPLLC MNKGYRRVPL FSRMGESLEP ASLFVYVWYV R

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sperm phospholipase Czeta from humans and cynomolgus monkeys triggers Ca2+ oscillations, activation and development of mouse oocytes." Cox L.J., Larman M.G., Saunders C.M., Hashimoto K., Swann K., Lai F.A. Reproduction 124:611-623(2002) [PubMed: 12416999] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION. Tissue: Testis.
[2]	"Cynomolgus monkey testicular cDNAs for discovery of novel human genes in the human genome sequence." Osada N., Hida M., Kusuda J., Tanuma R., Hirata M., Suto Y., Hirai M., Terao K., Sugano S., Hashimoto K. BMC Genomics 3:36-36(2002) [PubMed: 12498619] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB070109 mRNA. Translation: BAB63054.1. AB070108 mRNA. Translation: BAB63053.1.
3D structure databases
HSSP	HSSP built from PDB template 1DJX based on UniProtKB P10688.
ModBase	Search...
Phylogenomic databases
HOVERGEN	Q95JS1.
Family and domain databases
InterPro	IPR000008. C2_Ca-dep. IPR018029. C2_membr_targeting. IPR011992. EF-Hand_type. IPR018247. EF_HAND_1_Ca_BS. IPR018249. EF_HAND_2. IPR015359. Phospholipase_C_EF-hand-like. IPR001192. Phospholipase_C_Pinositol-sp_C. IPR000909. Phospholipase_C_Pinositol-sp_X. IPR001711. Phospholipase_C_Pinositol-sp_Y. IPR017946. PLC-like_Pdiesterase_TIM-brl. [Graphical view]
Gene3D	G3DSA:1.10.238.10. EF-Hand_type. 2 hits. G3DSA:3.20.20.190. PLC-like_Pdiesterase_TIM-brl. 1 hit.
Pfam	PF00168. C2. 1 hit. PF09279. efhand_like. 1 hit. PF00388. PI-PLC-X. 1 hit. PF00387. PI-PLC-Y. 1 hit. [Graphical view]
PRINTS	PR00390. PHPHLIPASEC.
ProDom	PD001202. PI_PLC_Y. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00239. C2. 1 hit. SM00148. PLCXc. 1 hit. SM00149. PLCYc. 1 hit. [Graphical view]
PROSITE	PS50004. C2. 1 hit. PS00018. EF_HAND_1. False negative. PS50222. EF_HAND_2. 1 hit. PS50007. PIPLC_X_DOMAIN. 1 hit. PS50008. PIPLC_Y_DOMAIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PLCZ1_MACFA

Accession

Primary (citable) accession number: Q95JS1
Secondary accession number(s): Q95JS0

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	December 1, 2001
Last modified:	September 22, 2009
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)