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Q95JH7 (AK1C1_MACFA) Reviewed, UniProtKB/Swiss-Prot

Last modified June 28, 2011. Version 50. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Aldo-keto reductase family 1 member C1 homolog
EC=1.-.-.-
Alternative name(s):
20-alpha-hydroxysteroid dehydrogenase
Short name=20-alpha-HSD
EC=1.1.1.149
Dihydrodiol dehydrogenase 1
Short name=DD-1
Short name=DD1
Indanol dehydrogenase
EC=1.1.1.112
Gene names
Name:AKR1C1
OrganismMacaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Taxonomic identifier9541 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Cercopithecidae › Cercopithecinae › Macaca

Protein attributes

Sequence length323 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Converts progesterone to its inactive form, 20-alpha-dihydroxyprogesterone (20-alpha-OHP). In the liver and intestine, may have a role in the transport of bile. May have a role in monitoring the intrahepatic bile acid concentration. May play a role in myelin formation. Can oxidize both 20-alpha- and 3-alpha-hydroxysteroids. Low activity towards 5-alpha-androstan-17-beta-ol-3-one. Can also act on non-steroidal substrates such as S-indan-1-ol, S-tetralol and 4-chromanol.

Catalytic activity

17-alpha,20-alpha-dihydroxypregn-4-en-3-one + NAD(P)+ = 17-alpha-hydroxyprogesterone + NAD(P)H.

Indan-1-ol + NAD(P)+ = indanone + NAD(P)H.

Enzyme regulation

Potently inhibited by benzbromarone and 3',3", 5', 5"-tetrabromophenolphthalein (TBPP).

Subunit structure

Monomer By similarity.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the aldo/keto reductase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 323323Aldo-keto reductase family 1 member C1 homolog
PRO_0000124634

Regions

Nucleotide binding13 – 2210NADP Potential
Nucleotide binding217 – 28064NADP By similarity

Sites

Active site551Proton donor By similarity
Binding site1171Substrate By similarity
Site541Important for substrate specificity By similarity
Site841Lowers pKa of active site Tyr By similarity

Sequences

Sequence LengthMass (Da)Tools
Q95JH7 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: D632ACC1C65D3744

FASTA32336,947
        10         20         30         40         50         60 
MDSKHQCVKL NDGHFMPVLG FGTYAPAEVP KNKALEATKL AIEAGFRHID SAHLYNNEEY 

        70         80         90        100        110        120 
VGLAIRSKIA DGTVKREDIF YTSKLWCNSH RPEFVRPALE RSLKNLQLDY VDLYLIHFPV 

       130        140        150        160        170        180 
SLKPGEELIP KDENGKLLFD TVDLCATWEA MEKCKDAGLA KSIGVSNFNR RQLEMILNKP 

       190        200        210        220        230        240 
GLKYKPVCNQ VECHPYLNQR KLLDFCKSKD IVLVAFSALG SHREKPWVDQ NSPVLLEDPV 

       250        260        270        280        290        300 
LCALAKKHKR TPALIALRYQ LQRGVVVLAK SYNEQRIREN MKVFEFQLTS EDMKAIDGLD 

       310        320 
RNIRYLTLDI FAGPPNYPFS DEY

« Hide

References

[1]"Molecular characterization of two monkey dihydrodiol dehydrogenases."
Higaki Y., Kamiya T., Usami N., Shintani S., Shiraishi H., Ishikura S., Yamamoto I., Hara A.
Drug Metab. Pharmacokinet. 17:348-356(2002) [PubMed: 15618685] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION, PROTEIN SEQUENCE OF 2-26; 40-123; 137-172; 271-282 AND 295-323.
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AB070209 mRNA. Translation: BAB63206.1.

3D structure databases

ProteinModelPortalQ95JH7.
SMRQ95JH7. Positions 2-323.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

HOVERGENHBG000020.

Enzyme and pathway databases

BRENDA1.3.1.20. 3121.

Family and domain databases

InterProIPR001395. Aldo/ket_red.
IPR018170. Aldo/ket_reductase_CS.
IPR020471. Aldo/keto_reductase_subgr.
IPR023210. NADP_OxRdtase_dom.
[Graphical view]
Gene3DG3DSA:3.20.20.100. Aldo/ket_red. 1 hit.
PANTHERPTHR11732. Aldo/ket_red. 1 hit.
PfamPF00248. Aldo_ket_red. 1 hit.
[Graphical view]
PIRSFPIRSF000097. AKR. 1 hit.
PRINTSPR00069. ALDKETRDTASE.
SUPFAMSSF51430. Aldo/ket_red. 1 hit.
PROSITEPS00798. ALDOKETO_REDUCTASE_1. 1 hit.
PS00062. ALDOKETO_REDUCTASE_2. 1 hit.
PS00063. ALDOKETO_REDUCTASE_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameAK1C1_MACFA
AccessionPrimary (citable) accession number: Q95JH7
Entry history
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: December 1, 2001
Last modified: June 28, 2011
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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