Reviewed, UniProtKB/Swiss-Prot Q95JH7 (AK1C1_MACFA)

Names and origin

Protein names

Recommended name:
    Aldo-keto reductase family 1 member C1 homolog
    EC=1.-.-.-
Alternative name(s):
    20-alpha-hydroxysteroid dehydrogenase
      Short name=20-alpha-HSD
    EC=1.1.1.149
    Indanol dehydrogenase
    EC=1.1.1.112
    Dihydrodiol dehydrogenase 1
      Short name=DD-1
      Short name=DD1

Gene names

Name:

AKR1C1

Organism

Macaca fascicularis (Crab eating macaque) (Cynomolgus monkey)

Taxonomic identifier

9541 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Cercopithecidae › Cercopithecinae › Macaca

Protein attributes

Sequence length	323 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Converts progesterone to its inactive form, 20-alpha-dihydroxyprogesterone (20-alpha-OHP). In the liver and intestine, may have a role in the transport of bile. May have a role in monitoring the intrahepatic bile acid concentration. May play a role in myelin formation. Can oxidize both 20-alpha- and 3-alpha-hydroxysteroids. Low activity towards 5-alpha-androstan-17-beta-ol-3-one. Can also act on non-steroidal substrates such as S-indan-1-ol, S-tetralol and 4-chromanol.
Catalytic activity	17-alpha,20-alpha-dihydroxypregn-4-en-3-one + NAD(P)+ = 17-alpha-hydroxyprogesterone + NAD(P)H. Indan-1-ol + NAD(P)+ = indanone + NAD(P)H.
Enzyme regulation	Potently inhibited by benzbromarone and 3',3", 5', 5"-tetrabromophenolphthalein (TBPP).
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the aldo/keto reductase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	bile acid metabolic process Inferred from sequence or structural similarity. Source: UniProtKB digestion Inferred from sequence or structural similarity. Source: UniProtKB oxidation reduction Inferred from sequence or structural similarity. Source: UniProtKB protein homooligomerization Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	cytosol Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	20-alpha-hydroxysteroid dehydrogenase activity Inferred from electronic annotation. Source: EC 3-alpha-hydroxysteroid dehydrogenase (B-specific) activity Inferred from sequence or structural similarity. Source: UniProtKB bile acid binding Inferred from sequence or structural similarity. Source: UniProtKB indanol dehydrogenase activity Inferred from electronic annotation. Source: EC trans-1,2-dihydrobenzene-1,2-diol dehydrogenase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 323	323	Aldo-keto reductase family 1 member C1 homolog		PRO_0000124634
Regions
	Nucleotide binding	13 – 22	10	NADP Potential
	Nucleotide binding	217 – 280	64	NADP By similarity
Sites
	Active site	55	1	Proton donor By similarity
	Binding site	117	1	Substrate By similarity
	Site	54	1	Important for substrate specificity By similarity
	Site	84	1	Lowers pKa of active site Tyr By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q95JH7-1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: D632ACC1C65D3744	FASTA	323	36,947
	10 20 30 40 50 60 MDSKHQCVKL NDGHFMPVLG FGTYAPAEVP KNKALEATKL AIEAGFRHID SAHLYNNEEY 70 80 90 100 110 120 VGLAIRSKIA DGTVKREDIF YTSKLWCNSH RPEFVRPALE RSLKNLQLDY VDLYLIHFPV 130 140 150 160 170 180 SLKPGEELIP KDENGKLLFD TVDLCATWEA MEKCKDAGLA KSIGVSNFNR RQLEMILNKP 190 200 210 220 230 240 GLKYKPVCNQ VECHPYLNQR KLLDFCKSKD IVLVAFSALG SHREKPWVDQ NSPVLLEDPV 250 260 270 280 290 300 LCALAKKHKR TPALIALRYQ LQRGVVVLAK SYNEQRIREN MKVFEFQLTS EDMKAIDGLD 310 320 RNIRYLTLDI FAGPPNYPFS DEY

References

[1]

"Molecular characterization of two monkey dihydrodiol dehydrogenases." Higaki Y., Kamiya T., Usami N., Shintani S., Shiraishi H., Ishikura S., Yamamoto I., Hara A. Drug Metab. Pharmacokinet. 17:348-356(2002) [PubMed: 15618685] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION, PROTEIN SEQUENCE OF 2-26; 40-123; 137-172; 271-282 AND 295-323. Tissue: Liver.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB070209 mRNA. Translation: BAB63206.1.
3D structure databases
SMR	Q95JH7. Positions 2-323.
ModBase	Search...
Phylogenomic databases
HOVERGEN	Q95JH7.
Enzyme and pathway databases
BRENDA	1.1.1.149. 3438. 1.3.1.20. 3438.
Family and domain databases
InterPro	IPR001395. Aldo/ket_red. IPR018170. Aldo/ket_reductase_CS. IPR020471. Aldo/keto_reductase_sg. [Graphical view]
Gene3D	G3DSA:3.20.20.100. Aldo/ket_red. 1 hit.
PANTHER	PTHR11732. Aldo/ket_red. 1 hit.
Pfam	PF00248. Aldo_ket_red. 1 hit. [Graphical view]
PRINTS	PR00069. ALDKETRDTASE.
PROSITE	PS00798. ALDOKETO_REDUCTASE_1. 1 hit. PS00062. ALDOKETO_REDUCTASE_2. 1 hit. PS00063. ALDOKETO_REDUCTASE_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AK1C1_MACFA

Accession

Primary (citable) accession number: Q95JH7

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	December 1, 2001
Last modified:	November 24, 2009
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)