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Q95748 (NDUS3_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NADH dehydrogenase [ubiquinone] iron-sulfur protein 3

EC=1.6.5.3
EC=1.6.99.3
Alternative name(s):
NADH dehydrogenase subunit 9
Gene names
Name:NAD9
Ordered Locus Names:AtMg00070
Encoded onMitochondrion
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length190 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone By similarity. HAMAP-Rule MF_01357

Catalytic activity

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out). HAMAP-Rule MF_01357

NADH + acceptor = NAD+ + reduced acceptor. HAMAP-Rule MF_01357

Subunit structure

Complex I is composed of at least 49 different subunits. This is a component of the iron-sulfur (IP) fragment of the enzyme.

Subcellular location

Mitochondrion inner membrane; Peripheral membrane protein; Matrix side Ref.5.

Sequence similarities

Belongs to the complex I 30 kDa subunit family.

RNA editing

Edited at positions 31, 56, 64, 100, 110, 133 and 147. Ref.3 Ref.4

Ontologies

Keywords
   Biological processElectron transport
Respiratory chain
Transport
   Cellular componentMembrane
Mitochondrion
Mitochondrion inner membrane
   Coding sequence diversityRNA editing
   LigandNAD
Ubiquinone
   Molecular functionOxidoreductase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentmitochondrial inner membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

respiratory chain

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionNADH dehydrogenase (ubiquinone) activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 190190NADH dehydrogenase [ubiquinone] iron-sulfur protein 3 HAMAP-Rule MF_01357
PRO_0000118637

Experimental info

Sequence conflict1301R → P in BAA11532. Ref.1
Sequence conflict1541Q → E in ABS50639. Ref.4
Sequence conflict1541Q → E in ABS50638. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Q95748 [UniParc].

Last modified January 16, 2004. Version 2.
Checksum: FCF70F540E71BC70

FASTA19022,880
        10         20         30         40         50         60 
MDNQFIFKYS WETLPKKWVK KMERSEHGNR FDTNTDYLFQ LLCFLKLHTY TRVQVLIDIC 

        70         80         90        100        110        120 
GVDYPSRKRR FEVVYNLLST RYNSRIRVQT SADEVTRISS VVSLFPSAGW WEREVWDMFG 

       130        140        150        160        170        180 
VSFINHPDLR RILTDYGFEG HPLRKDFPLS GYVQVRYDDP EKRVVSEPIE MTQEFRYFDF 

       190 
ASPWEQRSDG 

« Hide

References

« Hide 'large scale' references
[1]"Altered mitochondrial gene expression in a maternal distorted leaf mutant of Arabidopsis induced by chloroplast mutator."
Sakamoto W., Kondo H., Murata M., Motoyoshi F.
Plant Cell 8:1377-1390(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: cv. Landsberg erecta.
[2]"The mitochondrial genome of Arabidopsis thaliana contains 57 genes in 366,924 nucleotides."
Unseld M., Marienfeld J.R., Brandt P., Brennicke A.
Nat. Genet. 15:57-61(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]"RNA editing in Arabidopsis mitochondria effects 441 C to U changes in ORFs."
Giege P., Brennicke A.
Proc. Natl. Acad. Sci. U.S.A. 96:15324-15329(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], RNA EDITING.
[4]"Genetic architecture of mitochondrial editing in Arabidopsis thaliana."
Bentolila S., Elliott L.E., Hanson M.R.
Genetics 178:1693-1708(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 9-184, RNA EDITING.
Strain: cv. Columbia and cv. Landsberg erecta.
Tissue: Rosette leaf.
[5]"Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins."
Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H.
Plant Cell 16:241-256(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
Strain: cv. Landsberg erecta.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D82062 Genomic DNA. Translation: BAA11532.1. Sequence problems.
Y08501 Genomic DNA. Translation: CAA69753.3. Sequence problems.
EF488926 mRNA. Translation: ABS50638.1.
EF488927 mRNA. Translation: ABS50639.1.
RefSeqNP_085479.1. NC_001284.2.

3D structure databases

ProteinModelPortalQ95748.
SMRQ95748. Positions 56-149.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ95748. 4 interactions.

Protein family/group databases

TCDB3.D.1.6.3. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Proteomic databases

PaxDbQ95748.
PRIDEQ95748.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsATMG00070.1; ATMG00070.1; ATMG00070.
GeneID814568.
KEGGath:ArthMp007.

Organism-specific databases

TAIRATMG00070.

Phylogenomic databases

eggNOGCOG0852.
HOGENOMHOG000009797.
KOK03936.

Enzyme and pathway databases

BioCycARA:ATMG00070-MONOMER.
MetaCyc:ATMG00070-MONOMER.

Gene expression databases

GenevestigatorQ95748.

Family and domain databases

HAMAPMF_01357. NDH1_NuoC.
InterProIPR010218. NADH_DH_suC.
IPR001268. NADH_UbQ_OxRdtase_30kDa_su.
IPR020396. NADH_UbQ_OxRdtase_CS.
[Graphical view]
PfamPF00329. Complex1_30kDa. 1 hit.
[Graphical view]
ProDomPD001581. NADH_UbQ_OxRdtase_30kDa_su. 1 hit.
[Graphical view] [Entries sharing at least one domain]
TIGRFAMsTIGR01961. NuoC_fam. 1 hit.
PROSITEPS00542. COMPLEX1_30K. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNDUS3_ARATH
AccessionPrimary (citable) accession number: Q95748
Secondary accession number(s): A7KNI3, P93279
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 16, 2004
Last modified: June 11, 2014
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names