Reviewed, UniProtKB/Swiss-Prot Q95748 (NDUS3_ARATH)

Names and origin

Protein names

Recommended name:
    NADH-ubiquinone oxidoreductase 27 kDa subunit
    EC=1.6.5.3
    EC=1.6.99.3
Alternative name(s):
    Complex I-27kD
      Short name=CI-27kD
    NADH dehydrogenase subunit 9

Gene names

Name:	NAD9
Ordered Locus Names:	AtMg00070

Encoded on

Mitochondrion

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	190 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone By similarity.
Catalytic activity	NADH + ubiquinone = NAD+ + ubiquinol. NADH + acceptor = NAD+ + reduced acceptor.
Subunit structure	Complex I is composed of about 30 different subunits.
Subcellular location	Mitochondrion inner membrane; Peripheral membrane protein; Matrix side Ref.4.
Sequence similarities	Belongs to the complex I 30 kDa subunit family.
RNA editing	Edited at positions 31, 56, 64, 100, 110, 133 and 147. Ref.3

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Coding sequence diversity	RNA editing
Ligand	NAD Ubiquinone
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-KW respiratory chain Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 190	190	NADH-ubiquinone oxidoreductase 27 kDa subunit		PRO_0000118637
Experimental info
	Sequence conflict	130	1	R → P in BAA11532. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q95748-1 [UniParc]. Last modified January 16, 2004. Version 2. Checksum: FCF70F540E71BC70	FASTA	190	22,880
	10 20 30 40 50 60 MDNQFIFKYS WETLPKKWVK KMERSEHGNR FDTNTDYLFQ LLCFLKLHTY TRVQVLIDIC 70 80 90 100 110 120 GVDYPSRKRR FEVVYNLLST RYNSRIRVQT SADEVTRISS VVSLFPSAGW WEREVWDMFG 130 140 150 160 170 180 VSFINHPDLR RILTDYGFEG HPLRKDFPLS GYVQVRYDDP EKRVVSEPIE MTQEFRYFDF 190 ASPWEQRSDG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Altered mitochondrial gene expression in a maternal distorted leaf mutant of Arabidopsis induced by chloroplast mutator." Sakamoto W., Kondo H., Murata M., Motoyoshi F. Plant Cell 8:1377-1390(1996) [PubMed: 8776901] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Landsberg erecta.
[2]	"The mitochondrial genome of Arabidopsis thaliana contains 57 genes in 366,924 nucleotides." Unseld M., Marienfeld J.R., Brandt P., Brennicke A. Nat. Genet. 15:57-61(1997) [PubMed: 8988169] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"RNA editing in Arabidopsis mitochondria effects 441 C to U changes in ORFs." Giege P., Brennicke A. Proc. Natl. Acad. Sci. U.S.A. 96:15324-15329(1999) [PubMed: 10611383] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], RNA EDITING.
[4]	"Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins." Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H. Plant Cell 16:241-256(2004) [PubMed: 14671022] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBL GenBank DDBJ	D82062 Genomic DNA. Translation: BAA11532.1. Sequence problems. Y08501 Genomic DNA. Translation: CAA69753.3. Sequence problems.
IPI	IPI00529948.
RefSeq	NP_085479.1.
3D structure databases
SMR	Q95748. Positions 34-181.
ModBase	Search...
Protein-protein interaction databases
STRING	Q95748.
Protein family/group databases
TCDB	3.D.1.6.3. H+ or Na+-translocating NADH dehydrogenase (NDH) family.
Proteomic databases
PRIDE	Q95748.
Genome annotation databases
GeneID	814568.
GenomeReviews	Gene locus ATMG00070 in contig Y08501_GR.
KEGG	ath:ArthMp007.
Organism-specific databases
TAIR	AtMg00070.
Phylogenomic databases
HOGENOM	HBG727675.
PhylomeDB	Q95748.
Enzyme and pathway databases
BioCyc	MetaCyc:ATMG00070-MONOMER.
BRENDA	1.6.5.3. 302. 1.6.99.3. 302.
Gene expression databases
Genevestigator	Q95748.
GermOnline	ATMG00070. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR010218. NADH_DH_csu. IPR001268. NADH_UbQ_OxRdtase_30kDa_su. IPR020396. NADH_UbQ_OxRdtase_CS. [Graphical view]
Pfam	PF00329. Complex1_30kDa. 1 hit. [Graphical view]
ProDom	PD001581. NADH_UbQ_OxRdtase_30kDa_su. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01961. NuoC_fam. 1 hit.
PROSITE	PS00542. COMPLEX1_30K. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NDUS3_ARATH

Accession

Primary (citable) accession number: Q95748
Secondary accession number(s): P93279

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	January 16, 2004
Last modified:	February 9, 2010
This is version 77 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)