Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Major histocompatibility complex class I-related gene protein

Gene

MR1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Antigen-presenting molecule specialized in presenting microbial vitamin B metabolites. Involved in the development and expansion of a small population of T-cells expressing an invariant T-cell receptor alpha chain called mucosal-associated invariant T-cells (MAIT). MAIT lymphocytes are preferentially located in the gut lamina propria and therefore may be involved in monitoring commensal flora or serve as a distress signal. Expression and MAIT cell recognition seem to be ligand-dependent.2 Publications

Miscellaneous

MR1 is detected in an open versus folded conformation. Only the folded MR1 conformer activates MAIT cells.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei65Pathogen-derived ligand1

GO - Molecular functioni

  • MHC class I receptor activity Source: UniProtKB

GO - Biological processi

Keywordsi

Biological processImmunity, Innate immunity

Names & Taxonomyi

Protein namesi
Recommended name:
Major histocompatibility complex class I-related gene protein
Short name:
MHC class I-related gene protein
Alternative name(s):
Class I histocompatibility antigen-like protein
Gene namesi
Name:MR1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000153029.14
HGNCiHGNC:4975 MR1
MIMi600764 gene
neXtProtiNX_Q95460

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini23 – 302ExtracellularSequence analysisAdd BLAST280
Transmembranei303 – 323HelicalSequence analysisAdd BLAST21
Topological domaini324 – 341CytoplasmicSequence analysisAdd BLAST18

Keywords - Cellular componenti

Cell membrane, Endoplasmic reticulum, Membrane, MHC I, Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi65K → R: Fails to refold in the presence of 6-FP. 1
Mutagenesisi169Q → L: Able to activate mouse MAIT cells. 1 Publication1
Mutagenesisi283C → G: No effect on cell surface expression. 1 Publication1

Organism-specific databases

DisGeNETi3140
MalaCardsiMR1
OpenTargetsiENSG00000153029
PharmGKBiPA29309

Chemistry databases

DrugBankiDB00098 Anti-thymocyte Globulin (Rabbit)

Polymorphism and mutation databases

BioMutaiMR1
DMDMi74751679

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 221 PublicationAdd BLAST22
ChainiPRO_000034444123 – 341Major histocompatibility complex class I-related gene proteinAdd BLAST319

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi107N-linked (GlcNAc...) asparagine1 Publication1
Disulfide bondi120 ↔ 183PROSITE-ProRule annotation1 Publication
Disulfide bondi222 ↔ 278PROSITE-ProRule annotation1 Publication

Post-translational modificationi

N-glycosylated.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ95460
PeptideAtlasiQ95460
PRIDEiQ95460
ProteomicsDBi75731
75732 [Q95460-2]
75733 [Q95460-3]
75734 [Q95460-4]
75735 [Q95460-5]

PTM databases

iPTMnetiQ95460
PhosphoSitePlusiQ95460

Expressioni

Tissue specificityi

Ubiquitous.1 Publication

Gene expression databases

BgeeiENSG00000153029
CleanExiHS_MR1
GenevisibleiQ95460 HS

Organism-specific databases

HPAiHPA048304

Interactioni

Subunit structurei

Heterodimerizes with B2M, this interaction is required for surface expression. Associated with the peptide-loading complex, TAPBP, CALR and PDIA3. Isoform 3/MR1B homodimerizes and doesn't associates with B2M nor proteins of the peptide-loading complex.1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi109385, 23 interactors
DIPiDIP-59986N
IntActiQ95460, 2 interactors
STRINGi9606.ENSP00000356552

Structurei

Secondary structure

1341
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi25 – 36Combined sources12
Beta strandi39 – 41Combined sources3
Beta strandi43 – 50Combined sources8
Beta strandi53 – 59Combined sources7
Turni60 – 62Combined sources3
Beta strandi66 – 69Combined sources4
Helixi70 – 73Combined sources4
Beta strandi74 – 76Combined sources3
Helixi78 – 106Combined sources29
Beta strandi113 – 122Combined sources10
Beta strandi128 – 136Combined sources9
Beta strandi139 – 145Combined sources7
Turni146 – 149Combined sources4
Beta strandi150 – 155Combined sources6
Helixi156 – 166Combined sources11
Helixi169 – 180Combined sources12
Helixi182 – 193Combined sources12
Helixi195 – 198Combined sources4
Beta strandi205 – 212Combined sources8
Beta strandi214 – 216Combined sources3
Beta strandi218 – 230Combined sources13
Beta strandi233 – 238Combined sources6
Turni239 – 241Combined sources3
Helixi244 – 246Combined sources3
Beta strandi247 – 249Combined sources3
Beta strandi256 – 258Combined sources3
Beta strandi260 – 267Combined sources8
Beta strandi270 – 272Combined sources3
Beta strandi276 – 282Combined sources7
Beta strandi285 – 290Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4GUPX-ray3.20A/C23-292[»]
4L4TX-ray2.00A/C23-292[»]
4L4VX-ray1.90A/C23-292[»]
4LCWX-ray2.40A/C23-292[»]
4NQCX-ray2.50A/C23-292[»]
4NQDX-ray2.20A/C23-292[»]
4NQEX-ray2.10A/C23-292[»]
4PJ5X-ray2.00A/C23-292[»]
4PJ7X-ray2.50A/C23-292[»]
4PJ8X-ray3.30A23-292[»]
4PJ9X-ray2.00A23-292[»]
4PJAX-ray2.68A/C23-292[»]
4PJBX-ray2.85A/C23-292[»]
4PJCX-ray2.50A/C23-292[»]
4PJDX-ray2.78A/C23-292[»]
4PJEX-ray1.95A/C23-292[»]
4PJFX-ray2.45A/C23-292[»]
4PJGX-ray2.40A/C23-292[»]
4PJHX-ray2.00A/C23-292[»]
4PJIX-ray2.50A/C23-292[»]
4PJXX-ray2.25A/C23-292[»]
5D5MX-ray2.20A/C23-292[»]
5D7IX-ray2.00A/C23-292[»]
5D7JX-ray1.97C/E23-292[»]
5D7LX-ray3.40A/C23-292[»]
5U16X-ray2.00A/C23-292[»]
5U17X-ray2.15A/C23-292[»]
5U1RX-ray2.70A/C23-292[»]
5U2VX-ray2.20A/C23-292[»]
5U6QX-ray1.90A/C23-292[»]
5U72X-ray2.50A/C23-292[»]
ProteinModelPortaliQ95460
SMRiQ95460
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini203 – 299Ig-like C1-typeAdd BLAST97

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni23 – 201Ligand-bindingAdd BLAST179
Regioni23 – 109Alpha-1Add BLAST87
Regioni110 – 201Alpha-2Add BLAST92
Regioni202 – 293Alpha-3Add BLAST92
Regioni294 – 302Connecting peptide9

Domaini

The alpha-3 region and to a lesser extent the transmembrane and cytosolic domains regulate surface expression. The alpha-3 region mediates interaction with B2M (PubMed:23051753).1 Publication
The ligand-binding groove is ideally suited to present small organic compounds that can originate from vitamins rather than antigenic peptides.1 Publication

Sequence similaritiesi

Belongs to the MHC class I family.Curated

Keywords - Domaini

Immunoglobulin domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410II5V Eukaryota
ENOG4111K8F LUCA
GeneTreeiENSGT00740000114936
HOGENOMiHOG000296917
HOVERGENiHBG016709
InParanoidiQ95460
OMAiGYVDSHP
OrthoDBiEOG091G0K1X
PhylomeDBiQ95460
TreeFamiTF336617

Family and domain databases

Gene3Di2.60.40.10, 1 hit
3.30.500.10, 1 hit
InterProiView protein in InterPro
IPR007110 Ig-like_dom
IPR036179 Ig-like_dom_sf
IPR013783 Ig-like_fold
IPR003006 Ig/MHC_CS
IPR003597 Ig_C1-set
IPR011161 MHC_I-like_Ag-recog
IPR037055 MHC_I-like_Ag-recog_sf
IPR011162 MHC_I/II-like_Ag-recog
IPR001039 MHC_I_a_a1/a2
PfamiView protein in Pfam
PF07654 C1-set, 1 hit
PF00129 MHC_I, 1 hit
PRINTSiPR01638 MHCCLASSI
SMARTiView protein in SMART
SM00407 IGc1, 1 hit
SUPFAMiSSF48726 SSF48726, 1 hit
SSF54452 SSF54452, 1 hit
PROSITEiView protein in PROSITE
PS50835 IG_LIKE, 1 hit
PS00290 IG_MHC, 1 hit

Sequences (5)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q95460-1) [UniParc]FASTAAdd to basket
Also known as: MR1A

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGELMAFLLP LIIVLMVKHS DSRTHSLRYF RLGVSDPIHG VPEFISVGYV
60 70 80 90 100
DSHPITTYDS VTRQKEPRAP WMAENLAPDH WERYTQLLRG WQQMFKVELK
110 120 130 140 150
RLQRHYNHSG SHTYQRMIGC ELLEDGSTTG FLQYAYDGQD FLIFNKDTLS
160 170 180 190 200
WLAVDNVAHT IKQAWEANQH ELLYQKNWLE EECIAWLKRF LEYGKDTLQR
210 220 230 240 250
TEPPLVRVNR KETFPGVTAL FCKAHGFYPP EIYMTWMKNG EEIVQEIDYG
260 270 280 290 300
DILPSGDGTY QAWASIELDP QSSNLYSCHV EHCGVHMVLQ VPQESETIPL
310 320 330 340
VMKAVSGSIV LVIVLAGVGV LVWRRRPREQ NGAIYLPTPD R
Length:341
Mass (Da):39,366
Last modified:February 1, 1997 - v1
Checksum:i2990C1F3F0A1CAD9
GO
Isoform 2 (identifier: Q95460-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     110-154: Missing.

Show »
Length:296
Mass (Da):34,278
Checksum:i05ABC9CD4189F639
GO
Isoform 3 (identifier: Q95460-3) [UniParc]FASTAAdd to basket
Also known as: MR1B, MR1D

The sequence of this isoform differs from the canonical sequence as follows:
     203-294: Missing.

Show »
Length:249
Mass (Da):28,973
Checksum:iDED2E979686D6A8A
GO
Isoform 4 (identifier: Q95460-4) [UniParc]FASTAAdd to basket
Also known as: MR1C

The sequence of this isoform differs from the canonical sequence as follows:
     202-220: EPPLVRVNRKETFPGVTAL → GKEKEKASFPHCLNNCFYT
     221-341: Missing.

Show »
Length:220
Mass (Da):25,933
Checksum:i529688A56159C475
GO
Isoform 5 (identifier: Q95460-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     202-328: Missing.

Note: No experimental confirmation available.
Show »
Length:214
Mass (Da):25,190
Checksum:iB84A09E70B280E74
GO

Sequence cautioni

The sequence AAD01443 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti117M → V in BAF83063 (PubMed:14702039).Curated1
Sequence conflicti200R → G in BAD96630 (Ref. 4) Curated1
Sequence conflicti315L → P in BAD96630 (Ref. 4) Curated1

Polymorphismi

Several individuals from different ethnic background were analyzed for polymorphism. MR1 was identical in all individuals analyzed, except one. MR1 is not polymorphic.

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_04560839H → R1 PublicationCorresponds to variant dbSNP:rs2236410EnsemblClinVar.1
Natural variantiVAR_04560963R → Q. Corresponds to variant dbSNP:rs3897433Ensembl.1
Natural variantiVAR_04561077A → V. Corresponds to variant dbSNP:rs3897434Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_034755110 – 154Missing in isoform 2. 1 PublicationAdd BLAST45
Alternative sequenceiVSP_043479202 – 328Missing in isoform 5. 1 PublicationAdd BLAST127
Alternative sequenceiVSP_034756202 – 220EPPLV…GVTAL → GKEKEKASFPHCLNNCFYT in isoform 4. 1 PublicationAdd BLAST19
Alternative sequenceiVSP_034757203 – 294Missing in isoform 3. 2 PublicationsAdd BLAST92
Alternative sequenceiVSP_034758221 – 341Missing in isoform 4. 1 PublicationAdd BLAST121

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U22963 mRNA Translation: AAC50174.1
AJ249778 mRNA Translation: CAB77667.1
AK290374 mRNA Translation: BAF83063.1
AK304645 mRNA Translation: BAG65423.1
AK222910 mRNA Translation: BAD96630.1
AL356267 Genomic DNA No translation available.
CH471067 Genomic DNA Translation: EAW91097.1
CH471067 Genomic DNA Translation: EAW91098.1
CH471067 Genomic DNA Translation: EAW91095.1
BC012485 mRNA Translation: AAH12485.1
AF010446 mRNA Translation: AAD01442.1
AF010447 mRNA Translation: AAD01443.1 Different initiation.
AF031469 mRNA Translation: AAD01933.1
AF039526 Genomic DNA Translation: AAD02172.1
AH006983 Genomic DNA Translation: AAC72900.1
AF223407 Genomic DNA Translation: AAF40170.1
CCDSiCCDS1342.1 [Q95460-1]
CCDS53440.1 [Q95460-3]
CCDS53441.1 [Q95460-5]
CCDS53442.1 [Q95460-2]
PIRiA57136
RefSeqiNP_001181928.1, NM_001194999.1 [Q95460-2]
NP_001181929.1, NM_001195000.1 [Q95460-3]
NP_001181964.1, NM_001195035.1 [Q95460-5]
NP_001522.1, NM_001531.2 [Q95460-1]
UniGeneiHs.13500

Genome annotation databases

EnsembliENST00000282990; ENSP00000282990; ENSG00000153029 [Q95460-3]
ENST00000367579; ENSP00000356551; ENSG00000153029 [Q95460-2]
ENST00000367580; ENSP00000356552; ENSG00000153029 [Q95460-1]
ENST00000434571; ENSP00000388504; ENSG00000153029 [Q95460-5]
ENST00000614012; ENSP00000477563; ENSG00000153029 [Q95460-1]
ENST00000617803; ENSP00000478976; ENSG00000153029 [Q95460-3]
GeneIDi3140
KEGGihsa:3140
UCSCiuc001goq.2 human [Q95460-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiHMR1_HUMAN
AccessioniPrimary (citable) accession number: Q95460
Secondary accession number(s): A8K2V9
, B4E3B1, O97985, O97986, Q53GM1, Q95HB8, Q9MY23, Q9NPL2, Q9TQB3, Q9TQB9, Q9TQK3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: February 1, 1997
Last modified: June 20, 2018
This is version 159 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health