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Q95334 (AMPE_PIG) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamyl aminopeptidase

Short name=EAP
EC=3.4.11.7
Alternative name(s):
Aminopeptidase A
Short name=AP-A
CD_antigen=CD249
Gene names
Name:ENPEP
OrganismSus scrofa (Pig) [Reference proteome]
Taxonomic identifier9823 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus

Protein attributes

Sequence length942 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Release of N-terminal glutamate (and to a lesser extent aspartate) from a peptide.

Cofactor

Binds 1 zinc ion per subunit By similarity.

Subunit structure

Homodimer; disulfide-linked. Ref.1

Subcellular location

Membrane; Single-pass type II membrane protein.

Sequence similarities

Belongs to the peptidase M1 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 942942Glutamyl aminopeptidase
PRO_0000095097

Regions

Topological domain1 – 1414Cytoplasmic Potential
Transmembrane15 – 3521Helical; Signal-anchor for type II membrane protein; Potential
Topological domain36 – 942907Extracellular Potential
Region347 – 3515Substrate binding By similarity

Sites

Active site3841Proton acceptor By similarity
Metal binding3831Zinc; catalytic By similarity
Metal binding3871Zinc; catalytic By similarity
Metal binding4061Zinc; catalytic By similarity
Binding site2131Substrate By similarity
Site4691Transition state stabilizer By similarity

Amino acid modifications

Glycosylation1101N-linked (GlcNAc...) Potential
Glycosylation1141N-linked (GlcNAc...) Potential
Glycosylation1871N-linked (GlcNAc...) Potential
Glycosylation3141N-linked (GlcNAc...) Potential
Glycosylation3671N-linked (GlcNAc...) Potential
Glycosylation5571N-linked (GlcNAc...) Potential
Glycosylation5791N-linked (GlcNAc...) Potential
Glycosylation5871N-linked (GlcNAc...) Potential
Glycosylation5971N-linked (GlcNAc...) Potential
Glycosylation6321N-linked (GlcNAc...) Potential
Glycosylation6681N-linked (GlcNAc...) Potential
Glycosylation7531N-linked (GlcNAc...) Potential
Glycosylation7861N-linked (GlcNAc...) Potential
Glycosylation7911N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q95334 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: C531E639A588EF2E

FASTA942108,284
        10         20         30         40         50         60 
MSTDSKRYCI KTKHVAIICA AVVAVGLIVG LSVGLTRSCD SKDGGQGTTQ SPSHLPPTSS 

        70         80         90        100        110        120 
PPQDQGVCPA SEDESGNWRD FRLPDFINPV HYDLQVKPLL EQDTYTGTVN ISINVTSPTQ 

       130        140        150        160        170        180 
HLWLHLRETR ITQLPVLWRP SGEQVQVRRC FEYKKQEYVV VEAEEELAPN SGEGLYHLTM 

       190        200        210        220        230        240 
EFAGWLNGSL VGFYRTTYVE KGQIKSIAAT DHEPTDARKS FPCFDEPNKK ATYTISIIHP 

       250        260        270        280        290        300 
KEYKALSNMP VEKEESVDDI WTQTTFQKSV PMSTYLVCFA VHQFDSVTRT SRSGKPLTIY 

       310        320        330        340        350        360 
VQPEQKHTAE YAANITKSVF DYFEDYFAME YSLPKLDKIA IPDFGTGAME NWGLITYRET 

       370        380        390        400        410        420 
NLLYDPNESA SSNQQRVAAV VAHELVHQWF GNIVTMEWWE DLWLNEGFAS FFEFLGVDHA 

       430        440        450        460        470        480 
EKEWQMRDQI LLEDVLPVQE DDSLISSHPI VVTVSTPAEI TSVFDGISYS KGASILRMLE 

       490        500        510        520        530        540 
DWITPEKFQK GCQEYLKKFE FKNAKTSDFW EALEEASNLP VKEVMDTWTN QMGYPVLNVE 

       550        560        570        580        590        600 
DMRIISQKRF LLDPNANSSE PHSVFGYTWN IPVRWTNDNE STITIYNRSE TGGITLNSSN 

       610        620        630        640        650        660 
PNGNAFLKIN PDHIGFYRVN YEVSTWEWIA TNLSLNHKDF STADRASLID DAFALARAQL 

       670        680        690        700        710        720 
LNYKEALNLT KYLKMEDEYL PWQRVISAVT YIISMFEDDK ELYPMIEKYF RDQVKPIADS 

       730        740        750        760        770        780 
LGWNDNGDHL TKLLRASVLG FACKMGDSNA LNNASHLFEQ WLTGTVSLPV NLRLLVYRYG 

       790        800        810        820        830        840 
MQNSGNETSW NYTLKQYQET SLAQEKEKLL YGLASVKNVA LLSRYLDLLK DPNVIKSQDV 

       850        860        870        880        890        900 
FTVIRYISYN SYGKTMAWNW IQLNWEYLVN RYTLNDRNLG RIVTIAEPFN TELQLWQMES 

       910        920        930        940 
FFKRYPEAGA GEKPREQVLE TVKNNIEWLK QNRDTIRDWF FN 

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References

[1]"Proteolytic fragmentation reveals the oligomeric and domain structure of porcine aminopeptidase A."
Hesp J.R., Hooper N.M.
Biochemistry 36:3000-3007(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBUNIT.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U66371 mRNA. Translation: AAB07141.1.
RefSeqNP_999182.1. NM_214017.1.
UniGeneSsc.83.

3D structure databases

ProteinModelPortalQ95334.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9823.ENSSSCP00000009739.

Chemistry

BindingDBQ95334.
ChEMBLCHEMBL4868.

Protein family/group databases

MEROPSM01.003.

Proteomic databases

PaxDbQ95334.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID397080.
KEGGssc:397080.

Organism-specific databases

CTD2028.

Phylogenomic databases

eggNOGCOG0308.
HOGENOMHOG000106482.
HOVERGENHBG006616.
KOK11141.

Family and domain databases

InterProIPR024571. ERAP1-like_C_dom.
IPR001930. Peptidase_M1.
IPR014782. Peptidase_M1_N.
[Graphical view]
PANTHERPTHR11533. PTHR11533. 1 hit.
PfamPF11838. ERAP1_C. 1 hit.
PF01433. Peptidase_M1. 1 hit.
[Graphical view]
PRINTSPR00756. ALADIPTASE.
PROSITEPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PMAP-CutDBQ95334.

Entry information

Entry nameAMPE_PIG
AccessionPrimary (citable) accession number: Q95334
Entry history
Integrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: February 1, 1997
Last modified: February 19, 2014
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries