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Protein

Glutamyl aminopeptidase

Gene

ENPEP

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Release of N-terminal glutamate (and to a lesser extent aspartate) from a peptide.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei213SubstrateBy similarity1
Metal bindingi383Zinc; catalyticPROSITE-ProRule annotation1
Active sitei384Proton acceptorPROSITE-ProRule annotation1
Metal bindingi387Zinc; catalyticPROSITE-ProRule annotation1
Metal bindingi406Zinc; catalyticPROSITE-ProRule annotation1
Sitei469Transition state stabilizerBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminopeptidase, Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Calcium, Metal-binding, Zinc

Protein family/group databases

MEROPSiM01.003.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamyl aminopeptidase (EC:3.4.11.7)
Short name:
EAP
Alternative name(s):
Aminopeptidase A
Short name:
AP-A
CD_antigen: CD249
Gene namesi
Name:ENPEP
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
Proteomesi
  • UP000008227 Componenti: Unplaced

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 14CytoplasmicSequence analysisAdd BLAST14
Transmembranei15 – 35Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini36 – 942ExtracellularSequence analysisAdd BLAST907

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL4868.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000950971 – 942Glutamyl aminopeptidaseAdd BLAST942

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi110N-linked (GlcNAc...)Sequence analysis1
Glycosylationi114N-linked (GlcNAc...)Sequence analysis1
Glycosylationi187N-linked (GlcNAc...)Sequence analysis1
Glycosylationi314N-linked (GlcNAc...)Sequence analysis1
Glycosylationi367N-linked (GlcNAc...)Sequence analysis1
Glycosylationi557N-linked (GlcNAc...)Sequence analysis1
Glycosylationi579N-linked (GlcNAc...)Sequence analysis1
Glycosylationi587N-linked (GlcNAc...)Sequence analysis1
Glycosylationi597N-linked (GlcNAc...)Sequence analysis1
Glycosylationi632N-linked (GlcNAc...)Sequence analysis1
Glycosylationi668N-linked (GlcNAc...)Sequence analysis1
Glycosylationi753N-linked (GlcNAc...)Sequence analysis1
Glycosylationi786N-linked (GlcNAc...)Sequence analysis1
Glycosylationi791N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PeptideAtlasiQ95334.

Miscellaneous databases

PMAP-CutDBQ95334.

Interactioni

Subunit structurei

Homodimer; disulfide-linked.1 Publication

Chemistry databases

BindingDBiQ95334.

Structurei

3D structure databases

ProteinModelPortaliQ95334.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni347 – 351Substrate bindingBy similarity5

Sequence similaritiesi

Belongs to the peptidase M1 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000106482.
HOVERGENiHBG006616.
InParanoidiQ95334.
KOiK11141.

Family and domain databases

InterProiIPR033508. Aminopeptidase_A.
IPR024571. ERAP1-like_C_dom.
IPR001930. Peptidase_M1.
IPR014782. Peptidase_M1_N.
[Graphical view]
PANTHERiPTHR11533. PTHR11533. 1 hit.
PTHR11533:SF188. PTHR11533:SF188. 1 hit.
PfamiPF11838. ERAP1_C. 1 hit.
PF01433. Peptidase_M1. 1 hit.
[Graphical view]
PRINTSiPR00756. ALADIPTASE.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q95334-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTDSKRYCI KTKHVAIICA AVVAVGLIVG LSVGLTRSCD SKDGGQGTTQ
60 70 80 90 100
SPSHLPPTSS PPQDQGVCPA SEDESGNWRD FRLPDFINPV HYDLQVKPLL
110 120 130 140 150
EQDTYTGTVN ISINVTSPTQ HLWLHLRETR ITQLPVLWRP SGEQVQVRRC
160 170 180 190 200
FEYKKQEYVV VEAEEELAPN SGEGLYHLTM EFAGWLNGSL VGFYRTTYVE
210 220 230 240 250
KGQIKSIAAT DHEPTDARKS FPCFDEPNKK ATYTISIIHP KEYKALSNMP
260 270 280 290 300
VEKEESVDDI WTQTTFQKSV PMSTYLVCFA VHQFDSVTRT SRSGKPLTIY
310 320 330 340 350
VQPEQKHTAE YAANITKSVF DYFEDYFAME YSLPKLDKIA IPDFGTGAME
360 370 380 390 400
NWGLITYRET NLLYDPNESA SSNQQRVAAV VAHELVHQWF GNIVTMEWWE
410 420 430 440 450
DLWLNEGFAS FFEFLGVDHA EKEWQMRDQI LLEDVLPVQE DDSLISSHPI
460 470 480 490 500
VVTVSTPAEI TSVFDGISYS KGASILRMLE DWITPEKFQK GCQEYLKKFE
510 520 530 540 550
FKNAKTSDFW EALEEASNLP VKEVMDTWTN QMGYPVLNVE DMRIISQKRF
560 570 580 590 600
LLDPNANSSE PHSVFGYTWN IPVRWTNDNE STITIYNRSE TGGITLNSSN
610 620 630 640 650
PNGNAFLKIN PDHIGFYRVN YEVSTWEWIA TNLSLNHKDF STADRASLID
660 670 680 690 700
DAFALARAQL LNYKEALNLT KYLKMEDEYL PWQRVISAVT YIISMFEDDK
710 720 730 740 750
ELYPMIEKYF RDQVKPIADS LGWNDNGDHL TKLLRASVLG FACKMGDSNA
760 770 780 790 800
LNNASHLFEQ WLTGTVSLPV NLRLLVYRYG MQNSGNETSW NYTLKQYQET
810 820 830 840 850
SLAQEKEKLL YGLASVKNVA LLSRYLDLLK DPNVIKSQDV FTVIRYISYN
860 870 880 890 900
SYGKTMAWNW IQLNWEYLVN RYTLNDRNLG RIVTIAEPFN TELQLWQMES
910 920 930 940
FFKRYPEAGA GEKPREQVLE TVKNNIEWLK QNRDTIRDWF FN
Length:942
Mass (Da):108,284
Last modified:February 1, 1997 - v1
Checksum:iC531E639A588EF2E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U66371 mRNA. Translation: AAB07141.1.
RefSeqiNP_999182.1. NM_214017.1.
UniGeneiSsc.83.

Genome annotation databases

GeneIDi397080.
KEGGissc:397080.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U66371 mRNA. Translation: AAB07141.1.
RefSeqiNP_999182.1. NM_214017.1.
UniGeneiSsc.83.

3D structure databases

ProteinModelPortaliQ95334.
ModBaseiSearch...
MobiDBiSearch...

Chemistry databases

BindingDBiQ95334.
ChEMBLiCHEMBL4868.

Protein family/group databases

MEROPSiM01.003.

Proteomic databases

PeptideAtlasiQ95334.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi397080.
KEGGissc:397080.

Organism-specific databases

CTDi2028.

Phylogenomic databases

HOGENOMiHOG000106482.
HOVERGENiHBG006616.
InParanoidiQ95334.
KOiK11141.

Miscellaneous databases

PMAP-CutDBQ95334.

Family and domain databases

InterProiIPR033508. Aminopeptidase_A.
IPR024571. ERAP1-like_C_dom.
IPR001930. Peptidase_M1.
IPR014782. Peptidase_M1_N.
[Graphical view]
PANTHERiPTHR11533. PTHR11533. 1 hit.
PTHR11533:SF188. PTHR11533:SF188. 1 hit.
PfamiPF11838. ERAP1_C. 1 hit.
PF01433. Peptidase_M1. 1 hit.
[Graphical view]
PRINTSiPR00756. ALADIPTASE.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAMPE_PIG
AccessioniPrimary (citable) accession number: Q95334
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: February 1, 1997
Last modified: October 5, 2016
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.