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Protein

Xaa-Pro aminopeptidase 2

Gene

XPNPEP2

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Membrane-bound metalloprotease which catalyzes the removal of a penultimate prolyl residue from the N-termini of peptides, such as Arg-Pro-Pro. May play a role in the metabolism of the vasodilator bradykinin.3 Publications

Catalytic activityi

Release of any N-terminal amino acid, including proline, that is linked to proline, even from a dipeptide or tripeptide.3 Publications

Cofactori

Zn2+1 Publication

Enzyme regulationi

Inhibited by apstatin and the metal ion chelator EDTA (PubMed:8870669). Potently inhibited by the converting enzyme inhibitors cilazaprilat; enalaprilat; L155,212; ramiprilat and YS 980 (PubMed:1312513). Also inhibited to a lesser extent by indolaprilat; quinaprilat; spiraprilat; captopril and zofenoprilat (PubMed:1312513).2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei115SubstrateBy similarity1
Binding sitei429Substrate; via tele nitrogenBy similarity1
Metal bindingi449Manganese 2By similarity1
Metal bindingi449Zinc 1By similarity1
Metal bindingi460Zinc 1By similarity1
Metal bindingi460Zinc 2By similarity1
Metal bindingi523Zinc 2; via tele nitrogenBy similarity1
Binding sitei523Substrate; via tele nitrogenBy similarity1
Binding sitei532Substrate; via tele nitrogenBy similarity1
Metal bindingi554Zinc 2By similarity1
Binding sitei554SubstrateBy similarity1
Metal bindingi568Zinc 1By similarity1
Metal bindingi568Zinc 2By similarity1

GO - Molecular functioni

Keywordsi

Molecular functionAminopeptidase, Hydrolase, Metalloprotease, Protease
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-SSC-163125. Post-translational modification: synthesis of GPI-anchored proteins.

Protein family/group databases

MEROPSiM24.005.

Names & Taxonomyi

Protein namesi
Recommended name:
Xaa-Pro aminopeptidase 2 (EC:3.4.11.92 Publications)
Alternative name(s):
Aminoacylproline aminopeptidase
Membrane-bound aminopeptidase P
Short name:
Membrane-bound APP
Short name:
Membrane-bound AmP
Short name:
mAmP
X-Pro aminopeptidase 2
Gene namesi
Name:XPNPEP2
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
Proteomesi
  • UP000008227 Componenti: Chromosome X

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_000002683122 – 649Xaa-Pro aminopeptidase 2Add BLAST628
PropeptideiPRO_0000026832650 – 673Removed in mature formCuratedAdd BLAST24

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi34N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi48N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi64N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi277N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi290N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi294N-linked (GlcNAc...) asparagine1 Publication1
Lipidationi649GPI-anchor amidated alanine1 Publication1

Post-translational modificationi

N-glycosylated.1 Publication

Keywords - PTMi

Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PaxDbiQ95333.
PeptideAtlasiQ95333.
PRIDEiQ95333.

Expressioni

Tissue specificityi

Kidney.1 Publication

Gene expression databases

BgeeiENSSSCG00000012651.
ExpressionAtlasiQ95333. differential.
GenevisibleiQ95333. SS.

Interactioni

Subunit structurei

Homotrimer.1 Publication

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000013450.

Structurei

3D structure databases

ProteinModelPortaliQ95333.
SMRiQ95333.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M24B family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2413. Eukaryota.
COG0006. LUCA.
GeneTreeiENSGT00390000013970.
HOGENOMiHOG000255713.
HOVERGENiHBG002934.
InParanoidiQ95333.
KOiK14208.
OMAiYYQTIRE.
OrthoDBiEOG091G0A2Q.
TreeFamiTF314183.

Family and domain databases

CDDicd01085. APP. 1 hit.
Gene3Di3.40.350.10. 2 hits.
InterProiView protein in InterPro
IPR029149. Creatin/AminoP/Spt16_NTD.
IPR028980. Creatinase/Aminopeptidase_P_N.
IPR000587. Creatinase_N.
IPR000994. Pept_M24.
IPR033740. Pept_M24B.
IPR032416. Peptidase_M24_C.
IPR001131. Peptidase_M24B_aminopep-P_CS.
PfamiView protein in Pfam
PF01321. Creatinase_N. 1 hit.
PF00557. Peptidase_M24. 1 hit.
PF16188. Peptidase_M24_C. 1 hit.
SUPFAMiSSF53092. SSF53092. 1 hit.
SSF55920. SSF55920. 1 hit.
PROSITEiView protein in PROSITE
PS00491. PROLINE_PEPTIDASE. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q95333-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQACWGCYP WLVLICACAW GHPKSLNQRE DVRNCSTSPP YLPVTAVNTT
60 70 80 90 100
AQLTALREQM LTQNLSAYII PDTDAHMSEY IGECDQRRAW ITGFIGSAGI
110 120 130 140 150
AVVTERKAAL WTDSRYWTQA ERQMDCNWEL HKEVSTGHIV TWLLTEIPVG
160 170 180 190 200
GRVGFDPFLF SIDSWESYDV ALQDADRELV SITVNLVDLV WGSERPPLPN
210 220 230 240 250
APIYALQEAF AGSTWQEKVS NIRSQMQKHH ERPTAVLLSA LDETAWLFNL
260 270 280 290 300
RSSDIPYNPF FYSYTLLTDS SIRLFANKSR FSSETLQYLN SSCNSSMCVQ
310 320 330 340 350
LEDYSQIRDS IQAYTSGDVK IWIGTRYTSY GLYEVIPKEK LVEDDYSPVM
360 370 380 390 400
ITKAVKNSRE QALLKASHVR DAVAVIRYLA WLEKNVPTGT VDEFSGAKRV
410 420 430 440 450
EEFRGEEEFF SGPSFETISA SGLNAALAHY SPTKELHRKL SSDEMYLLDS
460 470 480 490 500
GGQYWDGTTD ITRTVHWGTP SAFQKEAYTR VLIGNIDLSR LVFPAATSGR
510 520 530 540 550
VVEAFARKAL WDVGLNYGHG TGHGIGNFLC VHEWPVGFQY GNIPMAEGMF
560 570 580 590 600
TSIEPGYYQD GEFGIRLEDV ALVVEAKTKY PGTYLTFEVV SLVPYDRKLI
610 620 630 640 650
DVSLLSPEQL QYLNRYYQAI REKVGPELQR RGLLEELSWL QRHTEPLSAR
660 670
AAPTTSLGSL MTVSALAILG WSV
Length:673
Mass (Da):75,756
Last modified:February 1, 1997 - v1
Checksum:iF480F50537CFD1B2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti530C → G AA sequence (PubMed:7744038).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U55039 mRNA. Translation: AAC48664.1.
PIRiS72440.
RefSeqiNP_001004048.1. NM_001004048.1.
XP_005673955.1. XM_005673898.2.
XP_013841867.1. XM_013986413.1.
UniGeneiSsc.16106.

Genome annotation databases

EnsembliENSSSCT00000013832; ENSSSCP00000013450; ENSSSCG00000012651.
GeneIDi445538.
KEGGissc:445538.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U55039 mRNA. Translation: AAC48664.1.
PIRiS72440.
RefSeqiNP_001004048.1. NM_001004048.1.
XP_005673955.1. XM_005673898.2.
XP_013841867.1. XM_013986413.1.
UniGeneiSsc.16106.

3D structure databases

ProteinModelPortaliQ95333.
SMRiQ95333.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000013450.

Protein family/group databases

MEROPSiM24.005.

Proteomic databases

PaxDbiQ95333.
PeptideAtlasiQ95333.
PRIDEiQ95333.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSSSCT00000013832; ENSSSCP00000013450; ENSSSCG00000012651.
GeneIDi445538.
KEGGissc:445538.

Organism-specific databases

CTDi7512.

Phylogenomic databases

eggNOGiKOG2413. Eukaryota.
COG0006. LUCA.
GeneTreeiENSGT00390000013970.
HOGENOMiHOG000255713.
HOVERGENiHBG002934.
InParanoidiQ95333.
KOiK14208.
OMAiYYQTIRE.
OrthoDBiEOG091G0A2Q.
TreeFamiTF314183.

Enzyme and pathway databases

ReactomeiR-SSC-163125. Post-translational modification: synthesis of GPI-anchored proteins.

Gene expression databases

BgeeiENSSSCG00000012651.
ExpressionAtlasiQ95333. differential.
GenevisibleiQ95333. SS.

Family and domain databases

CDDicd01085. APP. 1 hit.
Gene3Di3.40.350.10. 2 hits.
InterProiView protein in InterPro
IPR029149. Creatin/AminoP/Spt16_NTD.
IPR028980. Creatinase/Aminopeptidase_P_N.
IPR000587. Creatinase_N.
IPR000994. Pept_M24.
IPR033740. Pept_M24B.
IPR032416. Peptidase_M24_C.
IPR001131. Peptidase_M24B_aminopep-P_CS.
PfamiView protein in Pfam
PF01321. Creatinase_N. 1 hit.
PF00557. Peptidase_M24. 1 hit.
PF16188. Peptidase_M24_C. 1 hit.
SUPFAMiSSF53092. SSF53092. 1 hit.
SSF55920. SSF55920. 1 hit.
PROSITEiView protein in PROSITE
PS00491. PROLINE_PEPTIDASE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiXPP2_PIG
AccessioniPrimary (citable) accession number: Q95333
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: February 1, 1997
Last modified: June 7, 2017
This is version 117 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.