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Protein

Xaa-Pro aminopeptidase 2

Gene

XPNPEP2

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Is a key enzyme in the metabolism of the vasodilator bradykinin. In addition to bradykinin, AP-P also cleaves substance P and peptides of the pancreatic polypeptide family.

Catalytic activityi

Release of any N-terminal amino acid, including proline, that is linked to proline, even from a dipeptide or tripeptide.

Cofactori

Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi449 – 4491Manganese 2By similarity
Metal bindingi460 – 4601Manganese 1By similarity
Metal bindingi460 – 4601Manganese 2By similarity
Metal bindingi554 – 5541Manganese 1By similarity
Metal bindingi568 – 5681Manganese 1By similarity
Metal bindingi568 – 5681Manganese 2By similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Aminopeptidase, Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Manganese, Metal-binding

Protein family/group databases

MEROPSiM24.005.

Names & Taxonomyi

Protein namesi
Recommended name:
Xaa-Pro aminopeptidase 2 (EC:3.4.11.9)
Alternative name(s):
Aminoacylproline aminopeptidase
Membrane-bound aminopeptidase P
Short name:
Membrane-bound APP
Short name:
Membrane-bound AmP
Short name:
mAmP
X-Pro aminopeptidase 2
Gene namesi
Name:XPNPEP2
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
Proteomesi
  • UP000008227 Componenti: Chromosome X

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Sequence analysisAdd
BLAST
Chaini22 – 649628Xaa-Pro aminopeptidase 2PRO_0000026831Add
BLAST
Propeptidei650 – 67324Removed in mature formPRO_0000026832Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi34 – 341N-linked (GlcNAc...)1 Publication
Glycosylationi48 – 481N-linked (GlcNAc...)1 Publication
Glycosylationi64 – 641N-linked (GlcNAc...)1 Publication
Glycosylationi277 – 2771N-linked (GlcNAc...)1 Publication
Glycosylationi290 – 2901N-linked (GlcNAc...)1 Publication
Glycosylationi294 – 2941N-linked (GlcNAc...)1 Publication
Lipidationi649 – 6491GPI-anchor amidated alanine1 Publication

Keywords - PTMi

Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PaxDbiQ95333.
PeptideAtlasiQ95333.

Expressioni

Tissue specificityi

In the kidney microvillar membrane fractions.

Gene expression databases

BgeeiENSSSCG00000012651.
ExpressionAtlasiQ95333. differential.
GenevisibleiQ95333. SS.

Interactioni

Subunit structurei

Homotrimer.

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000013450.

Structurei

3D structure databases

ProteinModelPortaliQ95333.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M24B family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2413. Eukaryota.
COG0006. LUCA.
GeneTreeiENSGT00390000013970.
HOGENOMiHOG000255713.
HOVERGENiHBG002934.
InParanoidiQ95333.
KOiK14208.
OMAiSPEHLQY.
OrthoDBiEOG091G0A2Q.
TreeFamiTF314183.

Family and domain databases

Gene3Di3.40.350.10. 1 hit.
3.90.230.10. 1 hit.
InterProiIPR029149. Creatin/AminoP/Spt16_NTD.
IPR028980. Creatinase/Aminopeptidase_P_N.
IPR000587. Creatinase_N.
IPR000994. Pept_M24_structural-domain.
IPR032416. Peptidase_M24_C.
IPR001131. Peptidase_M24B_aminopep-P_CS.
[Graphical view]
PfamiPF01321. Creatinase_N. 1 hit.
PF00557. Peptidase_M24. 1 hit.
PF16188. Peptidase_M24_C. 1 hit.
[Graphical view]
SUPFAMiSSF53092. SSF53092. 1 hit.
SSF55920. SSF55920. 1 hit.
PROSITEiPS00491. PROLINE_PEPTIDASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q95333-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQACWGCYP WLVLICACAW GHPKSLNQRE DVRNCSTSPP YLPVTAVNTT
60 70 80 90 100
AQLTALREQM LTQNLSAYII PDTDAHMSEY IGECDQRRAW ITGFIGSAGI
110 120 130 140 150
AVVTERKAAL WTDSRYWTQA ERQMDCNWEL HKEVSTGHIV TWLLTEIPVG
160 170 180 190 200
GRVGFDPFLF SIDSWESYDV ALQDADRELV SITVNLVDLV WGSERPPLPN
210 220 230 240 250
APIYALQEAF AGSTWQEKVS NIRSQMQKHH ERPTAVLLSA LDETAWLFNL
260 270 280 290 300
RSSDIPYNPF FYSYTLLTDS SIRLFANKSR FSSETLQYLN SSCNSSMCVQ
310 320 330 340 350
LEDYSQIRDS IQAYTSGDVK IWIGTRYTSY GLYEVIPKEK LVEDDYSPVM
360 370 380 390 400
ITKAVKNSRE QALLKASHVR DAVAVIRYLA WLEKNVPTGT VDEFSGAKRV
410 420 430 440 450
EEFRGEEEFF SGPSFETISA SGLNAALAHY SPTKELHRKL SSDEMYLLDS
460 470 480 490 500
GGQYWDGTTD ITRTVHWGTP SAFQKEAYTR VLIGNIDLSR LVFPAATSGR
510 520 530 540 550
VVEAFARKAL WDVGLNYGHG TGHGIGNFLC VHEWPVGFQY GNIPMAEGMF
560 570 580 590 600
TSIEPGYYQD GEFGIRLEDV ALVVEAKTKY PGTYLTFEVV SLVPYDRKLI
610 620 630 640 650
DVSLLSPEQL QYLNRYYQAI REKVGPELQR RGLLEELSWL QRHTEPLSAR
660 670
AAPTTSLGSL MTVSALAILG WSV
Length:673
Mass (Da):75,756
Last modified:February 1, 1997 - v1
Checksum:iF480F50537CFD1B2
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti530 – 5301C → G AA sequence (PubMed:7744038).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U55039 mRNA. Translation: AAC48664.1.
PIRiS72440.
RefSeqiNP_001004048.1. NM_001004048.1.
XP_005673955.1. XM_005673898.2.
XP_013841867.1. XM_013986413.1.
UniGeneiSsc.16106.

Genome annotation databases

EnsembliENSSSCT00000013832; ENSSSCP00000013450; ENSSSCG00000012651.
GeneIDi445538.
KEGGissc:445538.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U55039 mRNA. Translation: AAC48664.1.
PIRiS72440.
RefSeqiNP_001004048.1. NM_001004048.1.
XP_005673955.1. XM_005673898.2.
XP_013841867.1. XM_013986413.1.
UniGeneiSsc.16106.

3D structure databases

ProteinModelPortaliQ95333.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000013450.

Protein family/group databases

MEROPSiM24.005.

Proteomic databases

PaxDbiQ95333.
PeptideAtlasiQ95333.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSSSCT00000013832; ENSSSCP00000013450; ENSSSCG00000012651.
GeneIDi445538.
KEGGissc:445538.

Organism-specific databases

CTDi7512.

Phylogenomic databases

eggNOGiKOG2413. Eukaryota.
COG0006. LUCA.
GeneTreeiENSGT00390000013970.
HOGENOMiHOG000255713.
HOVERGENiHBG002934.
InParanoidiQ95333.
KOiK14208.
OMAiSPEHLQY.
OrthoDBiEOG091G0A2Q.
TreeFamiTF314183.

Gene expression databases

BgeeiENSSSCG00000012651.
ExpressionAtlasiQ95333. differential.
GenevisibleiQ95333. SS.

Family and domain databases

Gene3Di3.40.350.10. 1 hit.
3.90.230.10. 1 hit.
InterProiIPR029149. Creatin/AminoP/Spt16_NTD.
IPR028980. Creatinase/Aminopeptidase_P_N.
IPR000587. Creatinase_N.
IPR000994. Pept_M24_structural-domain.
IPR032416. Peptidase_M24_C.
IPR001131. Peptidase_M24B_aminopep-P_CS.
[Graphical view]
PfamiPF01321. Creatinase_N. 1 hit.
PF00557. Peptidase_M24. 1 hit.
PF16188. Peptidase_M24_C. 1 hit.
[Graphical view]
SUPFAMiSSF53092. SSF53092. 1 hit.
SSF55920. SSF55920. 1 hit.
PROSITEiPS00491. PROLINE_PEPTIDASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiXPP2_PIG
AccessioniPrimary (citable) accession number: Q95333
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: February 1, 1997
Last modified: September 7, 2016
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.