Q95332 (BHMT1_PIG) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Betaine--homocysteine S-methyltransferase 1
EC=2.1.1.5

Gene names

Name:

BHMT

Organism

Sus scrofa (Pig) [Complete proteome]

Taxonomic identifier

9823 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus

Protein attributes

Sequence length	278 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the regulation of homocysteine metabolism. Converts betaine and homocysteine to dimethylglycine and methionine, respectively. This reaction is also required for the irreversible oxidation of choline.
Catalytic activity	Trimethylammonioacetate + L-homocysteine = dimethylglycine + L-methionine.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Amine and polyamine degradation; betaine degradation; sarcosine from betaine: step 1/2. Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (BhmT route): step 1/1.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm.
Tissue specificity	Found exclusively in liver and kidney. Ref.2
Sequence similarities	Contains 1 Hcy-binding domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	betaine-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: EC homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.1
	Chain	2 – ›278	›277	Betaine--homocysteine S-methyltransferase 1		PRO_0000114623
Regions
	Domain	11 – ›278	›268	Hcy-binding
Sites
	Metal binding	217	1	Zinc By similarity
Experimental info
	Non-terminal residue	278	1

Sequences

	Sequence		Length	Mass (Da)
	Q95332 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 58D3CAFEE7294CA3	FASTA	278	30,134
	10 20 30 40 50 60 MAPVGDKKAK KGILERLNSG EVVIGDGGFV FALEKRGYVK AGPWTPEAAV EHPEAVRQLH 70 80 90 100 110 120 REFLRAGSNV MQTFTFYASE DKLENRGNYV AEKISGQKVN EAACDIARQV ADEGDALVAG 130 140 150 160 170 180 GVSQTPSYLS CKSETEVKKV FRQQLEVFMK KNVDFLIAEY FEHVEEAVWA VEALKASGKP 190 200 210 220 230 240 VAATMCIGPE GDLHGVTPGQ CAVRLVKAGA SIVGVNCHFD PTISLQTVKL MKEGLQAAGL 250 260 270 KAHLMSQPLA YHTPDCGKQG FIDLPEFPFG LEPRVATR

References

[1]	"Purification, kinetic properties, and cDNA cloning of mammalian betaine-homocysteine methyltransferase." Garrow T.A. J. Biol. Chem. 271:22831-22838(1996) [PubMed: 8798461] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-273, PROTEIN SEQUENCE OF 2-22 AND 257-278. Tissue: Liver.
[2]	"Betaine-homocysteine methyltransferase expression in porcine and human tissues and chromosomal localization of the human gene." Sunden S.L.F., Renduchintala M.S., Park E.I., Miklasz S.D., Garrow T.A. Arch. Biochem. Biophys. 345:171-174(1997) [PubMed: 9281325] [Abstract] Cited for: TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U53421 mRNA. Translation: AAC48632.1.
UniGene	Ssc.16105. Ssc.82464.
3D structure databases
ProteinModelPortal	Q95332.
SMR	Q95332. Positions 10-278.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-246731.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
GeneTree	ENSGT00390000003122.
HOVERGEN	HBG080367.
OrthoDB	EOG4001JM.
Family and domain databases
InterPro	IPR017226. Betaine-hCys_S-MeTrfase_BHMT. IPR003726. S_MeTrfase. [Graphical view]
Gene3D	G3DSA:3.20.20.330. S_methyl_trans. 1 hit.
Pfam	PF02574. S-methyl_trans. 1 hit. [Graphical view]
PIRSF	PIRSF037505. Betaine_HMT. 1 hit.
SUPFAM	SSF82282. S_methyl_trans. 1 hit.
PROSITE	PS50970. HCY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BHMT1_PIG

Accession

Primary (citable) accession number: Q95332

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	January 23, 2007
Last modified:	July 27, 2011
This is version 65 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program