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Q95328 (CP17A_HORSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Steroid 17-alpha-hydroxylase/17,20 lyase

EC=1.14.99.9
EC=4.1.2.30
Alternative name(s):
17-alpha-hydroxyprogesterone aldolase
CYPXVII
Cytochrome P450 17A1
Cytochrome P450-C17
Short name=Cytochrome P450c17
Gene names
Name:CYP17A1
Synonyms:CYP17
OrganismEquus caballus (Horse) [Reference proteome]
Taxonomic identifier9796 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaPerissodactylaEquidaeEquus

Protein attributes

Sequence length508 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Conversion of pregnenolone and progesterone to their 17-alpha-hydroxylated products and subsequently to dehydroepiandrosterone (DHEA) and androstenedione. Catalyzes both the 17-alpha-hydroxylation and the 17,20-lyase reaction.

Catalytic activity

A C(21)-steroid + (reduced NADPH--hemoprotein reductase) + O2 = a 17-alpha-hydroxy-C(21)-steroid + (oxidized NADPH--hemoprotein reductase) + H2O.

17-alpha-hydroxyprogesterone = androst-4-ene-3,17-dione + acetaldehyde.

Cofactor

Heme group By similarity.

Pathway

Lipid metabolism; steroid biosynthesis.

Subcellular location

Membrane Potential.

Sequence similarities

Belongs to the cytochrome P450 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 508508Steroid 17-alpha-hydroxylase/17,20 lyase
PRO_0000051930

Sites

Metal binding4421Iron (heme axial ligand) By similarity

Experimental info

Sequence conflict3881D → E in BAA06350. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q95328 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: 71862C8B50AC4BE5

FASTA50857,394
        10         20         30         40         50         60 
MWELLAFLLL AIAYFFRPKV KCPGAKYPKS LPYLPLVGSL PFLPRHGHPH VNFFKLQKKY 

        70         80         90        100        110        120 
GPIYSLRMGT KTTVMVGHYQ LAKEVLIKKG KEFSGRPQVA TLNILSDNQK GVAFADHGAP 

       130        140        150        160        170        180 
WQLHRKLVRA AFALFKDGNQ KLEKIICHET SLLCDLLATQ NGQTIDLSSP LFLAVTNVIC 

       190        200        210        220        230        240 
WICFNSSYMK GDPALETMQN YHKGILETLE KDNVVDIFPA LKIFPNKSLE KMRHCVNIRN 

       250        260        270        280        290        300 
ELLSKIFEKH KENFNSDSIT SMLDLLIQAK KNSDNNNTGP DQDSKLLSDK HILATIGDIF 

       310        320        330        340        350        360 
GAGVETTTSV VKWIVAFLLH DPQLKKKIQE EIDQNVGFSR TPTLSDRNRL LLLEATIREV 

       370        380        390        400        410        420 
LRIRPVAPML IPHKALVDSS IGEFAVDDGT NVIINLWALH HNEKEWHQPD RFMPERFLDP 

       430        440        450        460        470        480 
TGSQLISPSL SYLPFGAGPR SCIGELLARQ ELFLFTAWLL QRFNLEVPDD GQLPSLEGHP 

       490        500 
TAVFLIDSFK VKINVRQAWR EAQAEGST 

« Hide

References

[1]"Molecular cloning and nucleotide sequence of equine testicular cytochrome P-450 steroid 17alpha-hydroxylase/C17,20-lyase messenger ribonucleic acid."
Hasegawa T., Mukoyama H., Yoshida S., Takahashi M.
(In) Sharp D.C., Bazer F.W. (eds.); Biology of Reproduction Monograph Series 1: Equine Reproduction, pp.VI:615-622, The Society for the Study of Reproduction, Madison (1995)
Cited for: NUCLEOTIDE SEQUENCE.
Tissue: Testis.
[2]"Exon/intron structure of equine P450c17."
Hasegawa T.
Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
Strain: Thoroughbred.
Tissue: Leukocyte.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D30688 mRNA. Translation: BAA06350.1.
D88184 Genomic DNA. Translation: BAA13551.1.
RefSeqNP_001075992.1. NM_001082523.1.
UniGeneEca.12075.

3D structure databases

ProteinModelPortalQ95328.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSECAT00000011028; ENSECAP00000008577; ENSECAG00000010022.
GeneID100034232.
KEGGecb:100034232.

Organism-specific databases

CTD1586.

Phylogenomic databases

eggNOGCOG2124.
GeneTreeENSGT00750000117317.
HOGENOMHOG000036991.
HOVERGENHBG106944.
InParanoidQ95328.
KOK00512.
OrthoDBEOG7RBZ85.
TreeFamTF105095.

Enzyme and pathway databases

UniPathwayUPA00062.

Family and domain databases

Gene3D1.10.630.10. 1 hit.
InterProIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00463. EP450I.
PR00385. P450.
SUPFAMSSF48264. SSF48264. 1 hit.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCP17A_HORSE
AccessionPrimary (citable) accession number: Q95328
Secondary accession number(s): Q29483
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: February 1, 1997
Last modified: April 16, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways