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Q95328

- CP17A_HORSE

UniProt

Q95328 - CP17A_HORSE

Protein

Steroid 17-alpha-hydroxylase/17,20 lyase

Gene

CYP17A1

Organism
Equus caballus (Horse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 99 (01 Oct 2014)
      Sequence version 1 (01 Feb 1997)
      Previous versions | rss
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    Functioni

    Conversion of pregnenolone and progesterone to their 17-alpha-hydroxylated products and subsequently to dehydroepiandrosterone (DHEA) and androstenedione. Catalyzes both the 17-alpha-hydroxylation and the 17,20-lyase reaction.

    Catalytic activityi

    A C(21)-steroid + (reduced NADPH--hemoprotein reductase) + O2 = a 17-alpha-hydroxy-C(21)-steroid + (oxidized NADPH--hemoprotein reductase) + H2O.
    17-alpha-hydroxyprogesterone = androst-4-ene-3,17-dione + acetaldehyde.

    Cofactori

    Heme group.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi442 – 4421Iron (heme axial ligand)By similarity

    GO - Molecular functioni

    1. 17-alpha-hydroxyprogesterone aldolase activity Source: UniProtKB-EC
    2. heme binding Source: UniProtKB
    3. iron ion binding Source: InterPro
    4. steroid 17-alpha-monooxygenase activity Source: UniProtKB

    GO - Biological processi

    1. glucocorticoid biosynthetic process Source: Ensembl
    2. hormone biosynthetic process Source: UniProtKB
    3. progesterone metabolic process Source: UniProtKB
    4. steroid metabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Lyase, Monooxygenase, Oxidoreductase

    Keywords - Biological processi

    Steroidogenesis

    Keywords - Ligandi

    Heme, Iron, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00062.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Steroid 17-alpha-hydroxylase/17,20 lyase (EC:1.14.99.9, EC:4.1.2.30)
    Alternative name(s):
    17-alpha-hydroxyprogesterone aldolase
    CYPXVII
    Cytochrome P450 17A1
    Cytochrome P450-C17
    Short name:
    Cytochrome P450c17
    Gene namesi
    Name:CYP17A1
    Synonyms:CYP17
    OrganismiEquus caballus (Horse)
    Taxonomic identifieri9796 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaPerissodactylaEquidaeEquus
    ProteomesiUP000002281: Chromosome 1

    Subcellular locationi

    Membrane Curated

    GO - Cellular componenti

    1. axon Source: Ensembl
    2. membrane Source: UniProtKB-SubCell
    3. mitochondrion Source: Ensembl
    4. neuronal cell body Source: Ensembl

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 508508Steroid 17-alpha-hydroxylase/17,20 lyasePRO_0000051930Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliQ95328.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Phylogenomic databases

    eggNOGiCOG2124.
    GeneTreeiENSGT00750000117317.
    HOGENOMiHOG000036991.
    HOVERGENiHBG106944.
    InParanoidiQ95328.
    KOiK00512.
    OrthoDBiEOG7RBZ85.
    TreeFamiTF105095.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PRINTSiPR00463. EP450I.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q95328-1 [UniParc]FASTAAdd to Basket

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    MWELLAFLLL AIAYFFRPKV KCPGAKYPKS LPYLPLVGSL PFLPRHGHPH    50
    VNFFKLQKKY GPIYSLRMGT KTTVMVGHYQ LAKEVLIKKG KEFSGRPQVA 100
    TLNILSDNQK GVAFADHGAP WQLHRKLVRA AFALFKDGNQ KLEKIICHET 150
    SLLCDLLATQ NGQTIDLSSP LFLAVTNVIC WICFNSSYMK GDPALETMQN 200
    YHKGILETLE KDNVVDIFPA LKIFPNKSLE KMRHCVNIRN ELLSKIFEKH 250
    KENFNSDSIT SMLDLLIQAK KNSDNNNTGP DQDSKLLSDK HILATIGDIF 300
    GAGVETTTSV VKWIVAFLLH DPQLKKKIQE EIDQNVGFSR TPTLSDRNRL 350
    LLLEATIREV LRIRPVAPML IPHKALVDSS IGEFAVDDGT NVIINLWALH 400
    HNEKEWHQPD RFMPERFLDP TGSQLISPSL SYLPFGAGPR SCIGELLARQ 450
    ELFLFTAWLL QRFNLEVPDD GQLPSLEGHP TAVFLIDSFK VKINVRQAWR 500
    EAQAEGST 508
    Length:508
    Mass (Da):57,394
    Last modified:February 1, 1997 - v1
    Checksum:i71862C8B50AC4BE5
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti388 – 3881D → E in BAA06350. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D30688 mRNA. Translation: BAA06350.1.
    D88184 Genomic DNA. Translation: BAA13551.1.
    RefSeqiNP_001075992.1. NM_001082523.1.
    UniGeneiEca.12075.

    Genome annotation databases

    EnsembliENSECAT00000011028; ENSECAP00000008577; ENSECAG00000010022.
    GeneIDi100034232.
    KEGGiecb:100034232.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D30688 mRNA. Translation: BAA06350.1 .
    D88184 Genomic DNA. Translation: BAA13551.1 .
    RefSeqi NP_001075992.1. NM_001082523.1.
    UniGenei Eca.12075.

    3D structure databases

    ProteinModelPortali Q95328.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSECAT00000011028 ; ENSECAP00000008577 ; ENSECAG00000010022 .
    GeneIDi 100034232.
    KEGGi ecb:100034232.

    Organism-specific databases

    CTDi 1586.

    Phylogenomic databases

    eggNOGi COG2124.
    GeneTreei ENSGT00750000117317.
    HOGENOMi HOG000036991.
    HOVERGENi HBG106944.
    InParanoidi Q95328.
    KOi K00512.
    OrthoDBi EOG7RBZ85.
    TreeFami TF105095.

    Enzyme and pathway databases

    UniPathwayi UPA00062 .

    Family and domain databases

    Gene3Di 1.10.630.10. 1 hit.
    InterProi IPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view ]
    Pfami PF00067. p450. 1 hit.
    [Graphical view ]
    PRINTSi PR00463. EP450I.
    PR00385. P450.
    SUPFAMi SSF48264. SSF48264. 1 hit.
    PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and nucleotide sequence of equine testicular cytochrome P-450 steroid 17alpha-hydroxylase/C17,20-lyase messenger ribonucleic acid."
      Hasegawa T., Mukoyama H., Yoshida S., Takahashi M.
      (In) Sharp D.C., Bazer F.W. (eds.); Biology of Reproduction Monograph Series 1: Equine Reproduction, pp.VI:615-622, The Society for the Study of Reproduction, Madison (1995)
      Cited for: NUCLEOTIDE SEQUENCE.
      Tissue: Testis.
    2. "Exon/intron structure of equine P450c17."
      Hasegawa T.
      Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: Thoroughbred.
      Tissue: Leukocyte.

    Entry informationi

    Entry nameiCP17A_HORSE
    AccessioniPrimary (citable) accession number: Q95328
    Secondary accession number(s): Q29483
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: February 1, 1997
    Last modified: October 1, 2014
    This is version 99 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3