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Protein

Steroid 17-alpha-hydroxylase/17,20 lyase

Gene

CYP17A1

Organism
Equus caballus (Horse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Conversion of pregnenolone and progesterone to their 17-alpha-hydroxylated products and subsequently to dehydroepiandrosterone (DHEA) and androstenedione. Catalyzes both the 17-alpha-hydroxylation and the 17,20-lyase reaction.

Catalytic activityi

A C(21)-steroid + (reduced NADPH--hemoprotein reductase) + O2 = a 17-alpha-hydroxy-C(21)-steroid + (oxidized NADPH--hemoprotein reductase) + H2O.
17-alpha-hydroxyprogesterone = androst-4-ene-3,17-dione + acetaldehyde.

Cofactori

hemeBy similarity

Pathway: steroid biosynthesis

This protein is involved in the pathway steroid biosynthesis, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway steroid biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi442 – 4421Iron (heme axial ligand)By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase, Monooxygenase, Oxidoreductase

Keywords - Biological processi

Steroidogenesis

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00062.

Names & Taxonomyi

Protein namesi
Recommended name:
Steroid 17-alpha-hydroxylase/17,20 lyase (EC:1.14.99.9, EC:4.1.2.30)
Alternative name(s):
17-alpha-hydroxyprogesterone aldolase
CYPXVII
Cytochrome P450 17A1
Cytochrome P450-C17
Short name:
Cytochrome P450c17
Gene namesi
Name:CYP17A1
Synonyms:CYP17
OrganismiEquus caballus (Horse)
Taxonomic identifieri9796 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaPerissodactylaEquidaeEquus
ProteomesiUP000002281 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 508508Steroid 17-alpha-hydroxylase/17,20 lyasePRO_0000051930Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi9796.ENSECAP00000008577.

Structurei

3D structure databases

ProteinModelPortaliQ95328.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiCOG2124.
HOGENOMiHOG000036991.
HOVERGENiHBG106944.
InParanoidiQ95328.
KOiK00512.
OrthoDBiEOG7RBZ85.
TreeFamiTF105095.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q95328-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWELLAFLLL AIAYFFRPKV KCPGAKYPKS LPYLPLVGSL PFLPRHGHPH
60 70 80 90 100
VNFFKLQKKY GPIYSLRMGT KTTVMVGHYQ LAKEVLIKKG KEFSGRPQVA
110 120 130 140 150
TLNILSDNQK GVAFADHGAP WQLHRKLVRA AFALFKDGNQ KLEKIICHET
160 170 180 190 200
SLLCDLLATQ NGQTIDLSSP LFLAVTNVIC WICFNSSYMK GDPALETMQN
210 220 230 240 250
YHKGILETLE KDNVVDIFPA LKIFPNKSLE KMRHCVNIRN ELLSKIFEKH
260 270 280 290 300
KENFNSDSIT SMLDLLIQAK KNSDNNNTGP DQDSKLLSDK HILATIGDIF
310 320 330 340 350
GAGVETTTSV VKWIVAFLLH DPQLKKKIQE EIDQNVGFSR TPTLSDRNRL
360 370 380 390 400
LLLEATIREV LRIRPVAPML IPHKALVDSS IGEFAVDDGT NVIINLWALH
410 420 430 440 450
HNEKEWHQPD RFMPERFLDP TGSQLISPSL SYLPFGAGPR SCIGELLARQ
460 470 480 490 500
ELFLFTAWLL QRFNLEVPDD GQLPSLEGHP TAVFLIDSFK VKINVRQAWR

EAQAEGST
Length:508
Mass (Da):57,394
Last modified:February 1, 1997 - v1
Checksum:i71862C8B50AC4BE5
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti388 – 3881D → E in BAA06350 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D30688 mRNA. Translation: BAA06350.1.
D88184 Genomic DNA. Translation: BAA13551.1.
RefSeqiNP_001075992.1. NM_001082523.1.
UniGeneiEca.12075.

Genome annotation databases

GeneIDi100034232.
KEGGiecb:100034232.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D30688 mRNA. Translation: BAA06350.1.
D88184 Genomic DNA. Translation: BAA13551.1.
RefSeqiNP_001075992.1. NM_001082523.1.
UniGeneiEca.12075.

3D structure databases

ProteinModelPortaliQ95328.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9796.ENSECAP00000008577.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100034232.
KEGGiecb:100034232.

Organism-specific databases

CTDi1586.

Phylogenomic databases

eggNOGiCOG2124.
HOGENOMiHOG000036991.
HOVERGENiHBG106944.
InParanoidiQ95328.
KOiK00512.
OrthoDBiEOG7RBZ85.
TreeFamiTF105095.

Enzyme and pathway databases

UniPathwayiUPA00062.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning and nucleotide sequence of equine testicular cytochrome P-450 steroid 17alpha-hydroxylase/C17,20-lyase messenger ribonucleic acid."
    Hasegawa T., Mukoyama H., Yoshida S., Takahashi M.
    (In) Sharp D.C., Bazer F.W. (eds.); Biology of Reproduction Monograph Series 1: Equine Reproduction, pp.VI:615-622, The Society for the Study of Reproduction, Madison (1995)
    Cited for: NUCLEOTIDE SEQUENCE.
    Tissue: Testis.
  2. "Exon/intron structure of equine P450c17."
    Hasegawa T.
    Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: Thoroughbred.
    Tissue: Leukocyte.

Entry informationi

Entry nameiCP17A_HORSE
AccessioniPrimary (citable) accession number: Q95328
Secondary accession number(s): Q29483
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: February 1, 1997
Last modified: June 24, 2015
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.