Q95328 (CP17A_HORSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Steroid 17-alpha-hydroxylase/17,20 lyase
EC=1.14.99.9

Alternative name(s):
CYPXVII
Cytochrome P450 17A1
Cytochrome P450-C17
Short name=Cytochrome P450c17

Gene names

Name:	CYP17A1
Synonyms:	CYP17

Organism

Equus caballus (Horse) [Complete proteome]

Taxonomic identifier

9796 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Perissodactyla › Equidae › Equus

Protein attributes

Sequence length	508 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Conversion of pregnenolone and progesterone to their 17-alpha-hydroxylated products and subsequently to dehydroepiandrosterone (DHEA) and androstenedione. Catalyzes both the 17-alpha-hydroxylation and the 17,20-lyase reaction.
Catalytic activity	A steroid + AH2 + O2 = a 17-alpha-hydroxysteroid + A + H2O.
Cofactor	Heme group By similarity.
Pathway	Lipid metabolism; steroid biosynthesis.
Subcellular location	Membrane Potential.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Biological process	Steroidogenesis
Cellular component	Membrane
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	steroid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro steroid 17-alpha-monooxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 508	508	Steroid 17-alpha-hydroxylase/17,20 lyase		PRO_0000051930
Sites
	Metal binding	442	1	Iron (heme axial ligand) By similarity
Experimental info
	Sequence conflict	388	1	D → E in BAA06350. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q95328 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 71862C8B50AC4BE5	FASTA	508	57,394
	10 20 30 40 50 60 MWELLAFLLL AIAYFFRPKV KCPGAKYPKS LPYLPLVGSL PFLPRHGHPH VNFFKLQKKY 70 80 90 100 110 120 GPIYSLRMGT KTTVMVGHYQ LAKEVLIKKG KEFSGRPQVA TLNILSDNQK GVAFADHGAP 130 140 150 160 170 180 WQLHRKLVRA AFALFKDGNQ KLEKIICHET SLLCDLLATQ NGQTIDLSSP LFLAVTNVIC 190 200 210 220 230 240 WICFNSSYMK GDPALETMQN YHKGILETLE KDNVVDIFPA LKIFPNKSLE KMRHCVNIRN 250 260 270 280 290 300 ELLSKIFEKH KENFNSDSIT SMLDLLIQAK KNSDNNNTGP DQDSKLLSDK HILATIGDIF 310 320 330 340 350 360 GAGVETTTSV VKWIVAFLLH DPQLKKKIQE EIDQNVGFSR TPTLSDRNRL LLLEATIREV 370 380 390 400 410 420 LRIRPVAPML IPHKALVDSS IGEFAVDDGT NVIINLWALH HNEKEWHQPD RFMPERFLDP 430 440 450 460 470 480 TGSQLISPSL SYLPFGAGPR SCIGELLARQ ELFLFTAWLL QRFNLEVPDD GQLPSLEGHP 490 500 TAVFLIDSFK VKINVRQAWR EAQAEGST

References

[1]

"Molecular cloning and nucleotide sequence of equine testicular cytochrome P-450 steroid 17alpha-hydroxylase/C17,20-lyase messenger ribonucleic acid." Hasegawa T., Mukoyama H., Yoshida S., Takahashi M. (In) Sharp D.C., Bazer F.W. (eds.); Biology of Reproduction Monograph Series 1: Equine Reproduction, pp.VI:615-622, The Society for the Study of Reproduction, Madison (1995) Cited for: NUCLEOTIDE SEQUENCE. Tissue: Testis.

[2]

"Exon/intron structure of equine P450c17." Hasegawa T. Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: Thoroughbred. Tissue: Leukocyte.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D30688 mRNA. Translation: BAA06350.1. D88184 Genomic DNA. Translation: BAA13551.1.
RefSeq	NP_001075992.1. NM_001082523.1.
UniGene	Eca.12075.
3D structure databases
ProteinModelPortal	Q95328.
ModBase	Search...
Protein-protein interaction databases
STRING	Q95328.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSECAT00000011028; ENSECAP00000008577; ENSECAG00000010022.
GeneID	100034232.
KEGG	ecb:100034232.
Organism-specific databases
CTD	1586.
Phylogenomic databases
GeneTree	ENSGT00590000082797.
HOVERGEN	HBG106944.
InParanoid	Q95328.
OrthoDB	EOG4W9J45.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
KO	K00512.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CP17A_HORSE

Accession

Primary (citable) accession number: Q95328
Secondary accession number(s): Q29483

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	February 1, 1997
Last modified:	November 16, 2011
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program