Q95327 (MANBA_CAPHI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-mannosidase
EC=3.2.1.25

Alternative name(s):
Lysosomal beta A mannosidase
Mannanase
Short name=Mannase

Gene names

Name:

MANBA

Organism

Capra hircus (Goat)

Taxonomic identifier

9925 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Caprinae › Capra

Protein attributes

Sequence length	879 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Exoglycosidase that cleaves the single beta-linked mannose residue from the non-reducing end of all N-linked glycoprotein oligosaccharides.
Catalytic activity	Hydrolysis of terminal, non-reducing beta-D-mannose residues in beta-D-mannosides.
Pathway	Glycan metabolism; N-glycan degradation.
Subcellular location	Lysosome.
Tissue specificity	Found in spleen and to a lesser extent in liver. Not detected in kidney or brain.
Involvement in disease	Defects in MANBA cause beta-mannosidosis, a severe disorder that affects peripheral and central nervous system myelin resulting in tremor, nystagmus, ataxia and early death. The primary storage products associated with the enzyme deficiency are the trisaccharide Man-beta-1-4-GlcNAc-beta-1-4-GlcNAc and the disaccharide Man-beta-1-4-GlcNAc.
Sequence similarities	Belongs to the glycosyl hydrolase 2 family.

Ontologies

Keywords
Cellular component	Lysosome
Coding sequence diversity	Polymorphism
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	lysosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	beta-mannosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17	Potential
	Chain	18 – 879	862	Beta-mannosidase		PRO_0000012165
Sites
	Active site	457	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	35	1	N-linked (GlcNAc...) Potential
	Glycosylation	77	1	N-linked (GlcNAc...) Potential
	Glycosylation	297	1	N-linked (GlcNAc...) Potential
	Glycosylation	803	1	N-linked (GlcNAc...) Potential
Natural variations
	Natural variant	56	1	R → Q.
	Natural variant	340	1	T → S.

Sequences

	Sequence		Length	Mass (Da)
	Q95327 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 2B96F03596B480C0	FASTA	879	101,386
	10 20 30 40 50 60 MLLRLLLLLA PCGAGFATEV VSISLRGNWK IHNGNGSLQL PAAVPGCVHS ALFNKRIIKD 70 80 90 100 110 120 PYYRFNNLDY RWIALDNWTY IKKFKLHSDM SEWNKVNLVF EGIDTVAVVL LNSVPIGKTD 130 140 150 160 170 180 NMFRRYSFDI THMVKAVNII EVRFQSPVIY ANQRSERHTA YWVPPNCPPP VQDGECHVNF 190 200 210 220 230 240 IRKMQCSFGW DWGPSFPTQG IWKDVRIEAY NICHLNYFMF TPIYDNYMET WNLKIESSFD 250 260 270 280 290 300 VVSSKLVSGE AIVAIPELNI QQRNNIELRH GERTVKLFVK IDKAVIVETW WPHGHGNQTG 310 320 330 340 350 360 YDMTVTFELD GGLRFEKSAK VYFRTVELVE EPIQNSPGLT FYFKINGLPI FLKGSNWIPA 370 380 390 400 410 420 DSFQDRVTSD MLRLLLQSVV DANMNALRVW GGGIYEQDEF YELCDELGIM IWQDFMFACA 430 440 450 460 470 480 LYPTDEDFMD SVREEVTHQV RRLKSHPSII TWSGNNENEA ALMMGWYDTK PGYLHTYIKD 490 500 510 520 530 540 YVTLYVKNIR TIVLEGDQTR PFIISSPTNG AKTTAEGWLS PNPYDLNYGD VHFYDYMSDC 550 560 570 580 590 600 WNWRTFPKAR FVSEYGYQSW PSFSTLEKVS SEEDWSYESS FALHRQHLIN GNSEMLQQIE 610 620 630 640 650 660 LHFKLPNSAD QLRRFKDTLY LTQVMQAQCV KTETEFYRRS RNEIVDGKGH TMGALYWQLN 670 680 690 700 710 720 DIWQAPSWSS LEYGGKWKML HYFARRFFAP LLPVGFEDKD VLFIYGVSDL PSDHQMMLTV 730 740 750 760 770 780 RVHTWSSLEL VCSELTNPFV MKAGESVVLY SKPVPELLKG CPGCTRQSCV VSFYLSTDGE 790 800 810 820 830 840 LLSPINYHFL SSLKNAKGLH KANITATISQ QGNTFVFDLK TSAVAPFVWL DVGSIPGRFS 850 860 870 DNGFLMTEKT RTVFFYPWKP TSKSELEQSF HVTSLADTY

References

[1]

"Caprine beta-mannosidase: sequencing and characterization of the cDNA and identification of the molecular defect of caprine beta-mannosidosis." Leipprandt J.R., Kraemer S.A., Haithcock B.E., Chen H., Dyme J.L., Cavanagh K.T., Friderici K.H., Jones M.Z. Genomics 37:51-56(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Kidney.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U46067 mRNA. Translation: AAC48665.1.
UniGene	Chi.8280.
3D structure databases
ProteinModelPortal	Q95327.
ModBase	Search...
Protein family/group databases
CAZy	GH2. Glycoside Hydrolase Family 2.
Proteomic databases
PRIDE	Q95327.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG052404.
Enzyme and pathway databases
UniPathway	UPA00280.
Family and domain databases
Gene3D	2.60.40.320. 1 hit. 3.20.20.80. 2 hits.
InterPro	IPR008979. Galactose-bd-like. IPR013812. Glyco_hydro_2/20_Ig-like. IPR006104. Glyco_hydro_2_N. IPR006103. Glyco_hydro_2_TIM. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Pfam	PF02836. Glyco_hydro_2_C. 1 hit. PF02837. Glyco_hydro_2_N. 1 hit. [Graphical view]
SUPFAM	SSF49785. Gal_bind_like. 1 hit. SSF51445. Glyco_hydro_cat. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MANBA_CAPHI

Accession

Primary (citable) accession number: Q95327

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	February 1, 1997
Last modified:	May 1, 2013
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program