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Q95327

- MANBA_CAPHI

UniProt

Q95327 - MANBA_CAPHI

Protein

Beta-mannosidase

Gene

MANBA

Organism
Capra hircus (Goat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 81 (01 Oct 2014)
      Sequence version 1 (01 Feb 1997)
      Previous versions | rss
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    Functioni

    Exoglycosidase that cleaves the single beta-linked mannose residue from the non-reducing end of all N-linked glycoprotein oligosaccharides.

    Catalytic activityi

    Hydrolysis of terminal, non-reducing beta-D-mannose residues in beta-D-mannosides.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei457 – 4571Proton donorBy similarity

    GO - Molecular functioni

    1. beta-mannosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. mannan catabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Enzyme and pathway databases

    UniPathwayiUPA00280.

    Protein family/group databases

    CAZyiGH2. Glycoside Hydrolase Family 2.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-mannosidase (EC:3.2.1.25)
    Alternative name(s):
    Lysosomal beta A mannosidase
    Mannanase
    Short name:
    Mannase
    Gene namesi
    Name:MANBA
    OrganismiCapra hircus (Goat)
    Taxonomic identifieri9925 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeCaprinaeCapra

    Subcellular locationi

    GO - Cellular componenti

    1. lysosome Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Lysosome

    Pathology & Biotechi

    Involvement in diseasei

    Defects in MANBA cause beta-mannosidosis, a severe disorder that affects peripheral and central nervous system myelin resulting in tremor, nystagmus, ataxia and early death. The primary storage products associated with the enzyme deficiency are the trisaccharide Man-beta-1-4-GlcNAc-beta-1-4-GlcNAc and the disaccharide Man-beta-1-4-GlcNAc.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1717Sequence AnalysisAdd
    BLAST
    Chaini18 – 879862Beta-mannosidasePRO_0000012165Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi35 – 351N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi77 – 771N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi297 – 2971N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi803 – 8031N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PRIDEiQ95327.

    Expressioni

    Tissue specificityi

    Found in spleen and to a lesser extent in liver. Not detected in kidney or brain.

    Structurei

    3D structure databases

    ProteinModelPortaliQ95327.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 2 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    HOVERGENiHBG052404.
    KOiK01192.

    Family and domain databases

    Gene3Di2.60.120.260. 2 hits.
    2.60.40.320. 1 hit.
    3.20.20.80. 2 hits.
    InterProiIPR008979. Galactose-bd-like.
    IPR013812. Glyco_hydro_2/20_Ig-like.
    IPR006102. Glyco_hydro_2_Ig-like.
    IPR006104. Glyco_hydro_2_N.
    IPR006103. Glyco_hydro_2_TIM.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR028369. Mannanase.
    [Graphical view]
    PANTHERiPTHR10066:SF12. PTHR10066:SF12. 1 hit.
    PfamiPF02836. Glyco_hydro_2_C. 1 hit.
    PF02837. Glyco_hydro_2_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF49303. SSF49303. 3 hits.
    SSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q95327-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLLRLLLLLA PCGAGFATEV VSISLRGNWK IHNGNGSLQL PAAVPGCVHS    50
    ALFNKRIIKD PYYRFNNLDY RWIALDNWTY IKKFKLHSDM SEWNKVNLVF 100
    EGIDTVAVVL LNSVPIGKTD NMFRRYSFDI THMVKAVNII EVRFQSPVIY 150
    ANQRSERHTA YWVPPNCPPP VQDGECHVNF IRKMQCSFGW DWGPSFPTQG 200
    IWKDVRIEAY NICHLNYFMF TPIYDNYMET WNLKIESSFD VVSSKLVSGE 250
    AIVAIPELNI QQRNNIELRH GERTVKLFVK IDKAVIVETW WPHGHGNQTG 300
    YDMTVTFELD GGLRFEKSAK VYFRTVELVE EPIQNSPGLT FYFKINGLPI 350
    FLKGSNWIPA DSFQDRVTSD MLRLLLQSVV DANMNALRVW GGGIYEQDEF 400
    YELCDELGIM IWQDFMFACA LYPTDEDFMD SVREEVTHQV RRLKSHPSII 450
    TWSGNNENEA ALMMGWYDTK PGYLHTYIKD YVTLYVKNIR TIVLEGDQTR 500
    PFIISSPTNG AKTTAEGWLS PNPYDLNYGD VHFYDYMSDC WNWRTFPKAR 550
    FVSEYGYQSW PSFSTLEKVS SEEDWSYESS FALHRQHLIN GNSEMLQQIE 600
    LHFKLPNSAD QLRRFKDTLY LTQVMQAQCV KTETEFYRRS RNEIVDGKGH 650
    TMGALYWQLN DIWQAPSWSS LEYGGKWKML HYFARRFFAP LLPVGFEDKD 700
    VLFIYGVSDL PSDHQMMLTV RVHTWSSLEL VCSELTNPFV MKAGESVVLY 750
    SKPVPELLKG CPGCTRQSCV VSFYLSTDGE LLSPINYHFL SSLKNAKGLH 800
    KANITATISQ QGNTFVFDLK TSAVAPFVWL DVGSIPGRFS DNGFLMTEKT 850
    RTVFFYPWKP TSKSELEQSF HVTSLADTY 879
    Length:879
    Mass (Da):101,386
    Last modified:February 1, 1997 - v1
    Checksum:i2B96F03596B480C0
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti56 – 561R → Q.
    Natural varianti340 – 3401T → S.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U46067 mRNA. Translation: AAC48665.1.
    RefSeqiNP_001272620.1. NM_001285691.1.
    UniGeneiChi.8280.

    Genome annotation databases

    GeneIDi100750234.
    KEGGichx:100750234.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U46067 mRNA. Translation: AAC48665.1 .
    RefSeqi NP_001272620.1. NM_001285691.1.
    UniGenei Chi.8280.

    3D structure databases

    ProteinModelPortali Q95327.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH2. Glycoside Hydrolase Family 2.

    Proteomic databases

    PRIDEi Q95327.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 100750234.
    KEGGi chx:100750234.

    Organism-specific databases

    CTDi 4126.

    Phylogenomic databases

    HOVERGENi HBG052404.
    KOi K01192.

    Enzyme and pathway databases

    UniPathwayi UPA00280 .

    Family and domain databases

    Gene3Di 2.60.120.260. 2 hits.
    2.60.40.320. 1 hit.
    3.20.20.80. 2 hits.
    InterProi IPR008979. Galactose-bd-like.
    IPR013812. Glyco_hydro_2/20_Ig-like.
    IPR006102. Glyco_hydro_2_Ig-like.
    IPR006104. Glyco_hydro_2_N.
    IPR006103. Glyco_hydro_2_TIM.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR028369. Mannanase.
    [Graphical view ]
    PANTHERi PTHR10066:SF12. PTHR10066:SF12. 1 hit.
    Pfami PF02836. Glyco_hydro_2_C. 1 hit.
    PF02837. Glyco_hydro_2_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49303. SSF49303. 3 hits.
    SSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Caprine beta-mannosidase: sequencing and characterization of the cDNA and identification of the molecular defect of caprine beta-mannosidosis."
      Leipprandt J.R., Kraemer S.A., Haithcock B.E., Chen H., Dyme J.L., Cavanagh K.T., Friderici K.H., Jones M.Z.
      Genomics 37:51-56(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Kidney.

    Entry informationi

    Entry nameiMANBA_CAPHI
    AccessioniPrimary (citable) accession number: Q95327
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: February 1, 1997
    Last modified: October 1, 2014
    This is version 81 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3