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Protein

Beta-mannosidase

Gene

MANBA

Organism
Capra hircus (Goat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Exoglycosidase that cleaves the single beta-linked mannose residue from the non-reducing end of all N-linked glycoprotein oligosaccharides.

Catalytic activityi

Hydrolysis of terminal, non-reducing beta-D-mannose residues in beta-D-mannosides.

Pathway:iN-glycan degradation

This protein is involved in the pathway N-glycan degradation, which is part of Glycan metabolism.
View all proteins of this organism that are known to be involved in the pathway N-glycan degradation and in Glycan metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei457 – 4571Proton donorBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BRENDAi3.2.1.25. 1166.
UniPathwayiUPA00280.

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-mannosidase (EC:3.2.1.25)
Alternative name(s):
Lysosomal beta A mannosidase
Mannanase
Short name:
Mannase
Gene namesi
Name:MANBA
OrganismiCapra hircus (Goat)
Taxonomic identifieri9925 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeCaprinaeCapra

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Lysosome

Pathology & Biotechi

Involvement in diseasei

Defects in MANBA cause beta-mannosidosis, a severe disorder that affects peripheral and central nervous system myelin resulting in tremor, nystagmus, ataxia and early death. The primary storage products associated with the enzyme deficiency are the trisaccharide Man-beta-1-4-GlcNAc-beta-1-4-GlcNAc and the disaccharide Man-beta-1-4-GlcNAc.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1717Sequence AnalysisAdd
BLAST
Chaini18 – 879862Beta-mannosidasePRO_0000012165Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi35 – 351N-linked (GlcNAc...)Sequence Analysis
Glycosylationi77 – 771N-linked (GlcNAc...)Sequence Analysis
Glycosylationi297 – 2971N-linked (GlcNAc...)Sequence Analysis
Glycosylationi803 – 8031N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiQ95327.

Expressioni

Tissue specificityi

Found in spleen and to a lesser extent in liver. Not detected in kidney or brain.

Structurei

3D structure databases

ProteinModelPortaliQ95327.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 2 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG052404.
KOiK01192.

Family and domain databases

Gene3Di2.60.120.260. 2 hits.
2.60.40.320. 1 hit.
3.20.20.80. 2 hits.
InterProiIPR008979. Galactose-bd-like.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR028369. Mannanase.
[Graphical view]
PANTHERiPTHR10066:SF12. PTHR10066:SF12. 1 hit.
PfamiPF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 3 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q95327-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLRLLLLLA PCGAGFATEV VSISLRGNWK IHNGNGSLQL PAAVPGCVHS
60 70 80 90 100
ALFNKRIIKD PYYRFNNLDY RWIALDNWTY IKKFKLHSDM SEWNKVNLVF
110 120 130 140 150
EGIDTVAVVL LNSVPIGKTD NMFRRYSFDI THMVKAVNII EVRFQSPVIY
160 170 180 190 200
ANQRSERHTA YWVPPNCPPP VQDGECHVNF IRKMQCSFGW DWGPSFPTQG
210 220 230 240 250
IWKDVRIEAY NICHLNYFMF TPIYDNYMET WNLKIESSFD VVSSKLVSGE
260 270 280 290 300
AIVAIPELNI QQRNNIELRH GERTVKLFVK IDKAVIVETW WPHGHGNQTG
310 320 330 340 350
YDMTVTFELD GGLRFEKSAK VYFRTVELVE EPIQNSPGLT FYFKINGLPI
360 370 380 390 400
FLKGSNWIPA DSFQDRVTSD MLRLLLQSVV DANMNALRVW GGGIYEQDEF
410 420 430 440 450
YELCDELGIM IWQDFMFACA LYPTDEDFMD SVREEVTHQV RRLKSHPSII
460 470 480 490 500
TWSGNNENEA ALMMGWYDTK PGYLHTYIKD YVTLYVKNIR TIVLEGDQTR
510 520 530 540 550
PFIISSPTNG AKTTAEGWLS PNPYDLNYGD VHFYDYMSDC WNWRTFPKAR
560 570 580 590 600
FVSEYGYQSW PSFSTLEKVS SEEDWSYESS FALHRQHLIN GNSEMLQQIE
610 620 630 640 650
LHFKLPNSAD QLRRFKDTLY LTQVMQAQCV KTETEFYRRS RNEIVDGKGH
660 670 680 690 700
TMGALYWQLN DIWQAPSWSS LEYGGKWKML HYFARRFFAP LLPVGFEDKD
710 720 730 740 750
VLFIYGVSDL PSDHQMMLTV RVHTWSSLEL VCSELTNPFV MKAGESVVLY
760 770 780 790 800
SKPVPELLKG CPGCTRQSCV VSFYLSTDGE LLSPINYHFL SSLKNAKGLH
810 820 830 840 850
KANITATISQ QGNTFVFDLK TSAVAPFVWL DVGSIPGRFS DNGFLMTEKT
860 870
RTVFFYPWKP TSKSELEQSF HVTSLADTY
Length:879
Mass (Da):101,386
Last modified:February 1, 1997 - v1
Checksum:i2B96F03596B480C0
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti56 – 561R → Q.
Natural varianti340 – 3401T → S.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U46067 mRNA. Translation: AAC48665.1.
RefSeqiNP_001272620.1. NM_001285691.1.
UniGeneiChi.8280.

Genome annotation databases

GeneIDi100750234.
KEGGichx:100750234.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U46067 mRNA. Translation: AAC48665.1.
RefSeqiNP_001272620.1. NM_001285691.1.
UniGeneiChi.8280.

3D structure databases

ProteinModelPortaliQ95327.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Proteomic databases

PRIDEiQ95327.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100750234.
KEGGichx:100750234.

Organism-specific databases

CTDi4126.

Phylogenomic databases

HOVERGENiHBG052404.
KOiK01192.

Enzyme and pathway databases

UniPathwayiUPA00280.
BRENDAi3.2.1.25. 1166.

Family and domain databases

Gene3Di2.60.120.260. 2 hits.
2.60.40.320. 1 hit.
3.20.20.80. 2 hits.
InterProiIPR008979. Galactose-bd-like.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR028369. Mannanase.
[Graphical view]
PANTHERiPTHR10066:SF12. PTHR10066:SF12. 1 hit.
PfamiPF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 3 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Caprine beta-mannosidase: sequencing and characterization of the cDNA and identification of the molecular defect of caprine beta-mannosidosis."
    Leipprandt J.R., Kraemer S.A., Haithcock B.E., Chen H., Dyme J.L., Cavanagh K.T., Friderici K.H., Jones M.Z.
    Genomics 37:51-56(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Kidney.

Entry informationi

Entry nameiMANBA_CAPHI
AccessioniPrimary (citable) accession number: Q95327
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: February 1, 1997
Last modified: April 1, 2015
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.