Reviewed, UniProtKB/Swiss-Prot Q95323 (CAH4_BOVIN)

Names and origin

Protein names

Recommended name:
    Carbonic anhydrase 4
    EC=4.2.1.1
Alternative name(s):
    Carbonic anhydrase IV
      Short name=CA-IV
    Carbonate dehydratase IV

Gene names

Name:

CA4

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	312 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Reversible hydration of carbon dioxide. May stimulate the sodium/bicarbonate transporter activity of SLC4A4 By similarity.
Catalytic activity	H2CO3 = CO2 + H2O.
Cofactor	Zinc.
Subunit structure	Interacts with SLC4A4 By similarity.
Subcellular location	Cell membrane; Lipid-anchor › GPI-anchor.
Sequence similarities	Belongs to the alpha-carbonic anhydrase family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Signal
Ligand	Metal-binding Zinc
Molecular function	Lyase
PTM	Disulfide bond GPI-anchor Glycoprotein Lipoprotein
Gene Ontology (GO)
Biological process	one-carbon metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	anchored to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	carbonate dehydratase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	By similarity
	Chain	19 – 284	266	Carbonic anhydrase 4		PRO_0000004224
	Propeptide	285 – 312	28	Removed in mature form By similarity		PRO_0000004225
Sites
	Metal binding	115	1	Zinc; catalytic
	Metal binding	117	1	Zinc; catalytic
	Metal binding	140	1	Zinc; catalytic
Amino acid modifications
	Lipidation	284	1	GPI-anchor amidated serine By similarity
	Glycosylation	33	1	N-linked (GlcNAc...) Potential
	Glycosylation	152	1	N-linked (GlcNAc...) Potential
	Glycosylation	195	1	N-linked (GlcNAc...) Potential
	Glycosylation	265	1	N-linked (GlcNAc...) Potential
	Disulfide bond	24 ↔ 36		By similarity
	Disulfide bond	46 ↔ 229		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q95323-1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: BAEA320C09426351	FASTA	312	35,151
	10 20 30 40 50 60 MRLLLALLVL AAAPPQARAA SHWCYQIQVK PSNYTCLEPD EWEGSCQNNR QSPVNIVTAK 70 80 90 100 110 120 TQLDPNLGRF SFSGYNMKHQ WVVQNNGHTV MVLLENKPSI AGGGLSTRYQ ATQLHLHWSR 130 140 150 160 170 180 AMDRGSEHSF DGERFAMEMH IVHEKEKGLS GNASQNQFAE DEIAVLAFMV EDGSKNVNFQ 190 200 210 220 230 240 PLVEALSDIP RPNMNTTMKE GVSLFDLLPE EESLRHYFRY LGSLTTPTCD EKVVWTVFQK 250 260 270 280 290 300 PIQLHRDQIL AFSQKLFYDD QQKVNMTDNV RPVQSLGQRQ VFRSGAPGLL LAQPLPTLLA 310 PVLACLTVGF LR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Bos taurus carbonic anhydrase IV (bovine carbonic anhydrase IV) mRNA, complete cds." Tamai S. Submitted (MAY-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Holstein. Tissue: Kidney.
[2]	NIH - Mammalian Gene Collection (MGC) project Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Crossbred X Angus. Tissue: Ileum.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U58870 mRNA. Translation: AAB09466.1. BC142534 mRNA. Translation: AAI42535.1.
IPI	IPI00712612.
RefSeq	NP_776322.1.
UniGene	Bt.555
3D structure databases
HSSP	HSSP built from PDB template 1ZNC based on UniProtKB P22748.
SMR	Q95323. Positions 23-284.
ModBase	Search...
Protein-protein interaction databases
STRING	Q95323.
Genome annotation databases
Ensembl	ENSBTAT00000023909; ENSBTAP00000023909; ENSBTAG00000017969; Bos taurus. [Genome view]
GeneID	280741.
KEGG	bta:280741.
Organism-specific databases
CTD	280741.
Phylogenomic databases
HOVERGEN	Q95323.
OMA	QNNGHTV.
Enzyme and pathway databases
BRENDA	4.2.1.1. 251.
Family and domain databases
InterPro	IPR001148. Carbonic_anhydrase_a-class_cat. IPR018338. Carbonic_anhydrase_a-class_CS. IPR018343. Carbonic_anhydrase_CA4. [Graphical view]
Gene3D	G3DSA:3.10.200.10. Euk_COanhd. 1 hit.
PANTHER	PTHR18952:SF5. Carbonic_anhydrase_CA4. 1 hit. PTHR18952. Euk_COanhd. 1 hit.
Pfam	PF00194. Carb_anhydrase. 1 hit. [Graphical view]
ProDom	PD000865. Euk_COanhd. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00162. ALPHA_CA_1. False negative. PS51144. ALPHA_CA_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CAH4_BOVIN

Accession

Primary (citable) accession number: Q95323
Secondary accession number(s): A5PKL8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	February 1, 1997
Last modified:	November 3, 2009
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)