Q95254 (GHSR_PIG) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Growth hormone secretagogue receptor type 1
Short name=GHS-R

Alternative name(s):
GH-releasing peptide receptor
Short name=GHRP
Ghrelin receptor

Gene names

Name:

GHSR

Organism

Sus scrofa (Pig) [Reference proteome]

Taxonomic identifier

9823 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus

Protein attributes

Sequence length	366 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Receptor for ghrelin, coupled to G-alpha-11 proteins. Stimulates growth hormone secretion. Binds also other growth hormone releasing peptides (GHRP) (e.g. Met-enkephalin and GHRP-6) as well as non-peptide, low molecular weight secretagogues (e.g. L-692,429, MK-0677, adenosine).
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	Pituitary and hypothalamus.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	actin polymerization or depolymerization Inferred from sequence or structural similarity. Source: UniProtKB adult feeding behavior Inferred from electronic annotation. Source: Compara cellular response to insulin stimulus Inferred from electronic annotation. Source: Compara decidualization Inferred from sequence or structural similarity. Source: UniProtKB growth hormone secretion Inferred from electronic annotation. Source: Compara hormone-mediated signaling pathway Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of inflammatory response Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of interleukin-1 beta production Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of interleukin-6 biosynthetic process Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of tumor necrosis factor biosynthetic process Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of appetite Inferred from electronic annotation. Source: Compara positive regulation of insulin-like growth factor receptor signaling pathway Inferred from electronic annotation. Source: Compara positive regulation of multicellular organism growth Inferred from sequence or structural similarity. Source: UniProtKB regulation of synapse assembly Inferred from electronic annotation. Source: Compara response to food Inferred from electronic annotation. Source: Compara
Cellular_component	cell surface Inferred from sequence or structural similarity. Source: UniProtKB integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW membrane raft Inferred from sequence or structural similarity. Source: UniProtKB neuron projection Inferred from sequence or structural similarity. Source: UniProtKB plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	growth hormone secretagogue receptor activity Inferred from sequence or structural similarity. Source: UniProtKB growth hormone-releasing hormone receptor activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1A (identifier: Q95254-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 1B (identifier: Q95254-2)

The sequence of this isoform differs from the canonical sequence as follows:
     266-289: AVVVFAFILCWLPFHVGRYLFSKS → GGSQCALELSLPGPLHSSCLFSSP
     290-366: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 366	366	Growth hormone secretagogue receptor type 1		PRO_0000069481
Regions
	Topological domain	1 – 40	40	Extracellular Potential
	Transmembrane	41 – 66	26	Helical; Name=1; Potential
	Topological domain	67 – 72	6	Cytoplasmic Potential
	Transmembrane	73 – 96	24	Helical; Name=2; Potential
	Topological domain	97 – 117	21	Extracellular Potential
	Transmembrane	118 – 139	22	Helical; Name=3; Potential
	Topological domain	140 – 162	23	Cytoplasmic Potential
	Transmembrane	163 – 183	21	Helical; Name=4; Potential
	Topological domain	184 – 211	28	Extracellular Potential
	Transmembrane	212 – 235	24	Helical; Name=5; Potential
	Topological domain	236 – 263	28	Cytoplasmic Potential
	Transmembrane	264 – 285	22	Helical; Name=6; Potential
	Topological domain	286 – 302	17	Extracellular Potential
	Transmembrane	303 – 326	24	Helical; Name=7; Potential
	Topological domain	327 – 366	40	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	13	1	N-linked (GlcNAc...) Potential
	Glycosylation	27	1	N-linked (GlcNAc...) Potential
	Disulfide bond	116 ↔ 198		By similarity
Natural variations
	Alternative sequence	266 – 289	24	AVVVF…LFSKS → GGSQCALELSLPGPLHSSCL FSSP in isoform 1B.		VSP_001918
	Alternative sequence	290 – 366	77	Missing in isoform 1B.		VSP_001919

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1A [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 2C850B3EF61B7C1C	FASTA	366	41,195
	10 20 30 40 50 60 MWNATPSEEP GPNLTLPDLG WDAPPENDSL VEELLPLFPT PLLAGVTATC VALFVVGIAG 70 80 90 100 110 120 NLLTMLVVSR FREMRTTTNL YLSSMAFSDL LIFLCMPLDL FRLWQYRPWN LGNLLCKLFQ 130 140 150 160 170 180 FVSESCTYAT VLTITALSVE RYFAICFPLR AKVVVTKGRV KLVILVIWAV AFCSAGPIFV 190 200 210 220 230 240 LVGVEHDNGT DPRDTNECRA TEFAVRSGLL TVMVWVSSVF FFLPVFCLTV LYSLIGRKLW 250 260 270 280 290 300 RRKRGEAAVG SSLRDQNHKQ TVKMLAVVVF AFILCWLPFH VGRYLFSKSL EPGSVEIAQI 310 320 330 340 350 360 SQYCNLVSFV LFYLSAAINP ILYNIMSKKY RVAVFKLLGF EPFSQRKLST LKDESSRAWT ESSINT
	Isoform 1B [UniParc]. Checksum: BB248CB89687D813	FASTA	289	32,047
	10 20 30 40 50 60 MWNATPSEEP GPNLTLPDLG WDAPPENDSL VEELLPLFPT PLLAGVTATC VALFVVGIAG 70 80 90 100 110 120 NLLTMLVVSR FREMRTTTNL YLSSMAFSDL LIFLCMPLDL FRLWQYRPWN LGNLLCKLFQ 130 140 150 160 170 180 FVSESCTYAT VLTITALSVE RYFAICFPLR AKVVVTKGRV KLVILVIWAV AFCSAGPIFV 190 200 210 220 230 240 LVGVEHDNGT DPRDTNECRA TEFAVRSGLL TVMVWVSSVF FFLPVFCLTV LYSLIGRKLW 250 260 270 280 RRKRGEAAVG SSLRDQNHKQ TVKMLGGSQC ALELSLPGPL HSSCLFSSP

References

[1]

"A receptor in pituitary and hypothalamus that functions in growth hormone release." Howard A.D., Feighner S.D., Cully D.F., Arena J.P., Liberator P.A., Rosenblum C.I., Hamelin M., Hreniuk D.L., Palyha O.C., Anderson J., Paress P.S., Diaz C., Chou M., Liu K.K., McKee K.K., Pong S.-S., Chaung L.-Y., Elbrecht A. , Dashkevicz M., Heavens R., Rigby M., Sirinathsinghji D.J.S., Dean D.C., Melillo D.G., Patchett A.A., Nargund R., Griffin P.R., Demartino J.A., Gupta S.K., Schaeffer J.M., Smith R.G., van der Ploeg L.H.T. Science 273:974-977(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1A AND 1B). Strain: Yorkshire. Tissue: Pituitary.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U60178 mRNA. Translation: AAC48630.1. U60180 mRNA. Translation: AAC48631.1.
RefSeq	NP_999345.1. NM_214180.1.
UniGene	Ssc.16107.
3D structure databases
ProteinModelPortal	Q95254.
ModBase	Search...
Protein-protein interaction databases
STRING	9823.ENSSSCP00000012525.
Protein family/group databases
GPCRDB	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSSSCT00000012865; ENSSSCP00000012525; ENSSSCG00000011754.
GeneID	397372.
KEGG	ssc:397372.
Organism-specific databases
CTD	2693.
Phylogenomic databases
eggNOG	NOG303294.
GeneTree	ENSGT00700000104070.
HOGENOM	HOG000272571.
HOVERGEN	HBG006538.
KO	K04284.
OMA	MVWVSSV.
OrthoDB	EOG4FFD28.
Family and domain databases
InterPro	IPR003905. GHS-R. IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR01417. GHSRECEPTOR. PR00237. GPCRRHODOPSN.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	Q95254.
ChEMBL	CHEMBL2224.

Entry information

Entry name

GHSR_PIG

Accession

Primary (citable) accession number: Q95254
Secondary accession number(s): Q95255

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	May 1, 2013
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program