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Protein

Calpastatin

Gene

CAST

Organism
Ovis aries (Sheep)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

Specific inhibition of calpain (calcium-dependent cysteine protease). Plays a key role in postmortem tenderization of meat and have been proposed to be involved in muscle protein degradation in living tissue (By similarity).By similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Thiol protease inhibitor

Protein family/group databases

MEROPSiI27.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Calpastatin
Alternative name(s):
Calpain inhibitor
Gene namesi
Name:CAST
OrganismiOvis aries (Sheep)
Taxonomic identifieri9940 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeCaprinaeOvis
Proteomesi
  • UP000002356 Componenti: Unplaced

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 723723CalpastatinPRO_0000147636Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki32 – 32Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei50 – 501N6-acetyllysineBy similarity
Modified residuei87 – 871PhosphoserineBy similarity
Modified residuei137 – 1371PhosphothreonineBy similarity
Modified residuei224 – 2241PhosphoserineBy similarity
Modified residuei245 – 2451PhosphoserineBy similarity
Modified residuei367 – 3671PhosphoserineBy similarity
Modified residuei369 – 3691PhosphoserineBy similarity
Modified residuei376 – 3761PhosphoserineBy similarity
Modified residuei443 – 4431PhosphoserineBy similarity
Modified residuei519 – 5191PhosphoserineBy similarity
Modified residuei530 – 5301PhosphoserineBy similarity
Modified residuei578 – 5781PhosphoserineBy similarity
Modified residuei580 – 5801PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati171 – 22454Inhibitory domain 1Add
BLAST
Repeati307 – 35953Inhibitory domain 2Add
BLAST
Repeati449 – 50254Inhibitory domain 3Add
BLAST
Repeati586 – 64257Inhibitory domain 4Add
BLAST

Domaini

Each of the four flexible inhibitory domains can inhibit one calcium-bound calpain molecule by occupying both sides of the active site.By similarity

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

HOVERGENiHBG000183.
KOiK04281.

Family and domain databases

InterProiIPR026998. Calpastatin.
IPR001259. Prot_inh_calpain.
[Graphical view]
PANTHERiPTHR10077. PTHR10077. 1 hit.
PfamiPF00748. Calpain_inhib. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q95208-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNPTEAKAIP GSKQLEGPHS PNKKRHKKQA VKTEPEKKSQ STKPSVVHEK
60 70 80 90 100
KTQEVKPKEH TEPKSQPKHP SDTRSKHAPK EKAVSKSSEQ PPSEKSTKPK
110 120 130 140 150
TKSQDEISGG GKSAVPAVAA AASAEPADKN KESKLLTSAV PVESKPSKPS
160 170 180 190 200
AKSDMDTALD DLIDTLGEPE ETKEDTTTYT GPEVSDPMSS TYIEELGKRE
210 220 230 240 250
VTLPPKYREL LNKEEGIAGP PPDSSKPLGP NDAIDALSSD FTCSSPTADA
260 270 280 290 300
KKTEKEKSTE EALKAQSAGV IRSAAPPKEK RRKVEEDTMT EQALEALSAS
310 320 330 340 350
LGTRKPRPEL DPSSIKEVDE AKAKEEKVKK CGEDEERVPS EYRLKPATDK
360 370 380 390 400
DGKPLLPEAE EKPKPLSESE LIDELSEDFD RSKCKEKQSK PTEKNRGIPG
410 420 430 440 450
RCPRACARGC ASDLHVFCAV SSTHSSSSEG MVPDDAVEAL AGSLGKKEAD
460 470 480 490 500
PEDGKPVEDK VKEKAKEEDR EKLGEREETI PPDYRLEEAK DKDGKPLPPK
510 520 530 540 550
EVKEPLPPLS EDFLLDALSK DFTVPSDTSS PQFEDAKLSV VVSEVVSQTP
560 570 580 590 600
APTTQAAGPP RDSARDNKEL DDALDQLSDS LGQRQPDPDE HKPVEDKVKE
610 620 630 640 650
KAKAEHRDKL GERDDTIPPK YQHLLDDNKE GTPGKPKRSE SPRHQRSPRH
660 670 680 690 700
QRNLQVPRTP LTPSQGTWTA VPQLQKPQQT QQRTKTRSLL PSDKAPRNGG
710 720
KAKDSTKAKE ETSKPKADGK STS
Length:723
Mass (Da):78,999
Last modified:February 1, 1997 - v1
Checksum:i251EA851D50B8F00
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U66320 mRNA. Translation: AAB07483.1.
RefSeqiNP_001009788.1. NM_001009788.1.
UniGeneiOar.498.

Genome annotation databases

GeneIDi443364.
KEGGioas:443364.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U66320 mRNA. Translation: AAB07483.1.
RefSeqiNP_001009788.1. NM_001009788.1.
UniGeneiOar.498.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiI27.001.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi443364.
KEGGioas:443364.

Organism-specific databases

CTDi831.

Phylogenomic databases

HOVERGENiHBG000183.
KOiK04281.

Family and domain databases

InterProiIPR026998. Calpastatin.
IPR001259. Prot_inh_calpain.
[Graphical view]
PANTHERiPTHR10077. PTHR10077. 1 hit.
PfamiPF00748. Calpain_inhib. 4 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Sequence analysis of ovine myocardial calpastatin mRNA transcripts."
    Mainelli K., Sugimoto J., Fishkin A., Knezetic J.
    Submitted (SEP-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Heart.

Entry informationi

Entry nameiICAL_SHEEP
AccessioniPrimary (citable) accession number: Q95208
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: October 14, 2015
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.