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Protein

Amiloride-sensitive sodium channel subunit beta

Gene

SCNN1B

Organism
Canis lupus familiaris (Dog) (Canis familiaris)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Plays an essential role in electrolyte and blood pressure homeostasis, but also in airway surface liquid homeostasis, which is important for proper clearance of mucus. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception.By similarity

Enzyme regulationi

Activated by WNK1, WNK2, WNK3 and WNK4.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Sodium channel

Keywords - Biological processi

Ion transport, Sensory transduction, Sodium transport, Taste, Transport

Keywords - Ligandi

Sodium

Enzyme and pathway databases

ReactomeiR-CFA-2672351. Stimuli-sensing channels.

Names & Taxonomyi

Protein namesi
Recommended name:
Amiloride-sensitive sodium channel subunit beta
Alternative name(s):
Beta-NaCH
Epithelial Na(+) channel subunit beta
Short name:
Beta-ENaC
Nonvoltage-gated sodium channel 1 subunit beta
SCNEB
Gene namesi
Name:SCNN1B
OrganismiCanis lupus familiaris (Dog) (Canis familiaris)
Taxonomic identifieri9615 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaCanidaeCanis
Proteomesi
  • UP000002254 Componentsi: Chromosome 6, Unassembled WGS sequence

Subcellular locationi

  • Apical cell membrane By similarity; Multi-pass membrane protein By similarity
  • Cytoplasmic vesicle membrane By similarity

  • Note: Apical membrane of epithelial cells.By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 50CytoplasmicBy similarityAdd BLAST50
Transmembranei51 – 71Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini72 – 533ExtracellularBy similarityAdd BLAST462
Transmembranei534 – 554Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini555 – 641CytoplasmicBy similarityAdd BLAST87

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasmic vesicle, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001812671 – 641Amiloride-sensitive sodium channel subunit betaAdd BLAST641

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi141N-linked (GlcNAc...)Sequence analysis1
Glycosylationi261N-linked (GlcNAc...)Sequence analysis1
Modified residuei634PhosphoserineBy similarity1
Modified residuei636PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated on serine and threonine residues. Aldosterone and insulin increase the basal level of phosphorylation.By similarity
N-glycosylated. N-glycosylation is required for interaction with BPIFA1.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ95165.

Interactioni

Subunit structurei

Heterotrimer containing an alpha/SCNN1A, a beta/SCNN1B and a gamma/SCNN1G subunit. An additional delta/SCNN1D subunit exists only in some organisms and can replace the alpha/SCNN1A subunit to form an alternative channel with specific properties. Interacts with NEDD4 (via WW domains). Interacts with NEDD4L (via WW domains). Interacts with WWP1 (via WW domains). Interacts with WWP2 (via WW domains). Interacts with the full length immature form of PCSK9 (pro-PCSK9). Interacts (N-glycosylated) with BPIFA1; the interaction is direct and inhibits the proteolytic processing of SCNN1A and SCNN1G and the activation of ENaC.By similarity

Protein-protein interaction databases

STRINGi9615.ENSCAFP00000026101.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4294. Eukaryota.
ENOG410ZNFK. LUCA.
GeneTreeiENSGT00760000119120.
HOGENOMiHOG000236286.
KOiK04825.
OMAiCNDTQYK.
OrthoDBiEOG091G0KW6.
TreeFamiTF330663.

Family and domain databases

InterProiIPR004724. ENaC.
IPR001873. Na+channel_ASC.
IPR020903. Na+channel_ASC_CS.
[Graphical view]
PANTHERiPTHR11690. PTHR11690. 2 hits.
PfamiPF00858. ASC. 1 hit.
[Graphical view]
PRINTSiPR01078. AMINACHANNEL.
TIGRFAMsiTIGR00859. ENaC. 1 hit.
PROSITEiPS01206. ASC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q95165-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHLKKYLLKG LHRLQKGPGY SYKELLVWYC NNTNTHGPKR IICEGPKKKA
60 70 80 90 100
MWFLITLLFT SLVCWQWGVF IRTYLSWEVS VSLSLGFKTM DFPAVTICNA
110 120 130 140 150
SPFQYSKVKH LLRDLDELME AVLERILAPE HSDANATRTL NVTMWNYTPL
160 170 180 190 200
VLIDEQNPHC PVVLDLFGDI HNGSASSSPA PARSCTVHGC KVAMRLCSLN
210 220 230 240 250
GTVCTFRNFT SATQAVTEWY LLQATNIFSQ VPQRELVEMS YPAERLILAC
260 270 280 290 300
LFGAEPCSYR NFTSIFHPDY GNCYIFNWGM TEKALPSANP GAEFGLKLIL
310 320 330 340 350
DIGQEDYVPF LTSTAGARLM LHEQRSYPFI KDEGIYAMSG TETSIGVLVD
360 370 380 390 400
RLERKGEPYS QCTVNGSDVP VRNLYSDYNT TYSIQACIRS CFQDHMIQNC
410 420 430 440 450
SCAHYLYPLP RGERYCNNRE FPDWAYCYSH LRMSVAQRET CINMCKESCN
460 470 480 490 500
DTQYKMTISM ADWPSEASED WIFHVLSQER DQSTNITLSR KGVVKLNIYF
510 520 530 540 550
QEFNYRTIEE SAANNIVWLL SNLGGQFGFW MGGSVLCLIE FGEILIDFVW
560 570 580 590 600
ITIIKLVAFA KSLRQKRAQA RYAGPPPTVA ELVEAHTNFG FQPDVARPGP
610 620 630 640
DPGTYPDEQT LPIPGTPPPN YDSLRLQPLD VIESDSEGDA I
Length:641
Mass (Da):72,654
Last modified:April 29, 2015 - v2
Checksum:i5A040E85895D751E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti567R → Q in AAB09034 (PubMed:9720188).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAEX03004450 Genomic DNA. No translation available.
U68062 Genomic DNA. Translation: AAB09034.1.
RefSeqiNP_001300793.1. NM_001313864.1.
XP_005621477.1. XM_005621420.2.

Genome annotation databases

EnsembliENSCAFT00000028060; ENSCAFP00000026101; ENSCAFG00000017687.
GeneIDi489972.
KEGGicfa:489972.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAEX03004450 Genomic DNA. No translation available.
U68062 Genomic DNA. Translation: AAB09034.1.
RefSeqiNP_001300793.1. NM_001313864.1.
XP_005621477.1. XM_005621420.2.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9615.ENSCAFP00000026101.

Proteomic databases

PaxDbiQ95165.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSCAFT00000028060; ENSCAFP00000026101; ENSCAFG00000017687.
GeneIDi489972.
KEGGicfa:489972.

Organism-specific databases

CTDi6338.

Phylogenomic databases

eggNOGiKOG4294. Eukaryota.
ENOG410ZNFK. LUCA.
GeneTreeiENSGT00760000119120.
HOGENOMiHOG000236286.
KOiK04825.
OMAiCNDTQYK.
OrthoDBiEOG091G0KW6.
TreeFamiTF330663.

Enzyme and pathway databases

ReactomeiR-CFA-2672351. Stimuli-sensing channels.

Family and domain databases

InterProiIPR004724. ENaC.
IPR001873. Na+channel_ASC.
IPR020903. Na+channel_ASC_CS.
[Graphical view]
PANTHERiPTHR11690. PTHR11690. 2 hits.
PfamiPF00858. ASC. 1 hit.
[Graphical view]
PRINTSiPR01078. AMINACHANNEL.
TIGRFAMsiTIGR00859. ENaC. 1 hit.
PROSITEiPS01206. ASC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSCNNB_CANLF
AccessioniPrimary (citable) accession number: Q95165
Secondary accession number(s): F1PGD1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: April 29, 2015
Last modified: September 7, 2016
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.