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Protein

Excitatory amino acid transporter 3

Gene

SLC1A1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate. Can also transport L-cysteine. Functions as a symporter that transports one amino acid molecule together with two or three Na+ ions and one proton, in parallel with the counter-transport of one K+ ion. Mediates Cl- flux that is not coupled to amino acid transport; this avoids the accumulation of negative charges due to aspartate and Na+ symport. Plays an important role in L-glutamate and L-aspartate reabsorption in renal tubuli (By similarity). Plays a redundant role in the rapid removal of released glutamate from the synaptic cleft, which is essential for terminating the postsynaptic action of glutamate (By similarity). Negatively regulated by ARL6IP5 (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi362Sodium 1; via carbonyl oxygenBy similarity1
Metal bindingi364Sodium 2; via carbonyl oxygenBy similarity1
Metal bindingi366Sodium 1By similarity1
Binding sitei370AspartateBy similarity1
Binding sitei444AspartateBy similarity1
Metal bindingi451Sodium 1; via carbonyl oxygenBy similarity1
Binding sitei451AspartateBy similarity1
Metal bindingi455Sodium 1By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processAmino-acid transport, Symport, Transport
LigandChloride, Metal-binding, Potassium, Sodium

Names & Taxonomyi

Protein namesi
Recommended name:
Excitatory amino acid transporter 3
Alternative name(s):
Excitatory amino-acid carrier 1
Renal high affinity glutamate transporter EAAC1
Sodium-dependent glutamate/aspartate transporter 3
Solute carrier family 1 member 1
Gene namesi
Name:SLC1A1
Synonyms:EAAC1, EAAT3
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 18CytoplasmicCuratedAdd BLAST18
Transmembranei19 – 38HelicalSequence analysisAdd BLAST20
Topological domaini39 – 61ExtracellularCuratedAdd BLAST23
Transmembranei62 – 82HelicalSequence analysisAdd BLAST21
Topological domaini83 – 93CytoplasmicCuratedAdd BLAST11
Transmembranei94 – 114HelicalSequence analysisAdd BLAST21
Topological domaini115 – 205ExtracellularCuratedAdd BLAST91
Transmembranei206 – 229Helical; Name=4By similarityAdd BLAST24
Topological domaini230 – 238CytoplasmicCurated9
Transmembranei239 – 266Helical; Name=5By similarityAdd BLAST28
Topological domaini267 – 286ExtracellularCuratedAdd BLAST20
Transmembranei287 – 308Helical; Name=6By similarityAdd BLAST22
Topological domaini309 – 313CytoplasmicCurated5
Intramembranei314 – 344Discontinuously helicalBy similarityAdd BLAST31
Topological domaini345 – 353CytoplasmicCurated9
Transmembranei354 – 380Helical; Name=7By similarityAdd BLAST27
Topological domaini381 – 393ExtracellularCuratedAdd BLAST13
Intramembranei394 – 427Discontinuously helicalBy similarityAdd BLAST34
Topological domaini428 – 440ExtracellularCuratedAdd BLAST13
Transmembranei441 – 462Helical; Name=8By similarityAdd BLAST22
Topological domaini463 – 524CytoplasmicCuratedAdd BLAST62

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002020641 – 524Excitatory amino acid transporter 3Add BLAST524

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi178N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi195N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei517PhosphoserineBy similarity1
Modified residuei522PhosphoserineBy similarity1

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ95135.
PRIDEiQ95135.

Interactioni

Subunit structurei

Homotrimer (Probable). Interacts with ARL6IP5.By similarityCurated

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000025456.

Structurei

3D structure databases

ProteinModelPortaliQ95135.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni331 – 333Aspartate bindingBy similarity3
Regioni411 – 415Aspartate bindingBy similarity5

Domaini

Contains eight transmembrane regions plus two helical hairpins that dip into the membrane. These helical hairpin structures play an important role in the transport process. The first enters the membrane from the cytoplasmic side, the second one from the extracellular side. During the transport cycle, the regions involved in amino acid transport, and especially the helical hairpins, move vertically by about 15-18 Angstroms, alternating between exposure to the aqueous phase and reinsertion in the lipid bilayer. In contrast, the regions involved in trimerization do not move.By similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3787. Eukaryota.
COG1301. LUCA.
HOGENOMiHOG000208776.
HOVERGENiHBG000080.
InParanoidiQ95135.
KOiK05612.

Family and domain databases

Gene3Di1.10.3860.10. 1 hit.
InterProiView protein in InterPro
IPR001991. Na-dicarboxylate_symporter.
IPR018107. Na-dicarboxylate_symporter_CS.
PfamiView protein in Pfam
PF00375. SDF. 1 hit.
PRINTSiPR00173. EDTRNSPORT.
SUPFAMiSSF118215. SSF118215. 2 hits.
PROSITEiView protein in PROSITE
PS00713. NA_DICARBOXYL_SYMP_1. 1 hit.
PS00714. NA_DICARBOXYL_SYMP_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q95135-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKPARKGCD WKRFLRNNWL LLSTVVAVVL GIVIGVLVRE YSKLSNLEKF
60 70 80 90 100
YFSFPGEILM RMLKLVILPL IVSSMITGVA TLDSNVSGKI GLRAVVYYFC
110 120 130 140 150
TTLIAVILGI VLVVSIKPGV TQKVNEIDRT GNTPEVSTVD AMLDLIRNMF
160 170 180 190 200
PENLVQACFQ QYKTTREEVE LSEEPGTNST EATVTAIMTT AISKNKTKEY
210 220 230 240 250
KVVGMYSDGI NVLGLIVFCL VLGIVIGRKW EKGQILVDFF NALSDATMKI
260 270 280 290 300
VQIIMCYMPI GILFLIAGKI IEVEDWEIFR KLGLYMATVL SGLAIHSIVI
310 320 330 340 350
LPLIYFIIVR KNPFQFAMGM AQALLTALMI SSSSATLPVT FRCAEEKNRV
360 370 380 390 400
DKRITRFVLP VGATINMDGT ALYEAVAAVF IAQLNDLDLS VGQIITISVT
410 420 430 440 450
ATAASIGAAG VPQPGLVTMV IVLSAVGLPA EDVTLIIAVD WLLDRFRTMV
460 470 480 490 500
NVLGDAFGTG IVEKLSKKEL EQMDVSSEVN IVNPFTLEST ALDNEDSDTK
510 520
KSYVNGGFAV DKSDTISFTQ TSQF
Length:524
Mass (Da):57,297
Last modified:February 1, 1997 - v1
Checksum:i920256A70A302128
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U72534 mRNA. Translation: AAB16815.1.
PIRiS39017.
RefSeqiNP_777024.1. NM_174599.2.
UniGeneiBt.557.

Genome annotation databases

GeneIDi282353.
KEGGibta:282353.

Similar proteinsi

Entry informationi

Entry nameiEAA3_BOVIN
AccessioniPrimary (citable) accession number: Q95135
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: August 30, 2017
This is version 97 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families