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Protein

Monocyte differentiation antigen CD14

Gene

CD14

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Coreceptor for bacterial lipopolysaccharide. In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway. Binds electronegative LDL (LDL-) and mediates the cytokine release induced by LDL- (By similarity).By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Immunity, Inflammatory response, Innate immunity

Names & Taxonomyi

Protein namesi
Recommended name:
Monocyte differentiation antigen CD14
Alternative name(s):
Myeloid cell-specific leucine-rich glycoprotein
CD_antigen: CD14
Gene namesi
Name:CD14
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

  • Cell membrane By similarity; Lipid-anchorGPI-anchor By similarity
  • Secreted By similarity
  • Membrane raft By similarity
  • Golgi apparatus By similarity

  • Note: Soluble, secreted forms seem to exist. They may arise by cleavage of the GPI anchor.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Golgi apparatus, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence analysisAdd
BLAST
Chaini21 – 373353Monocyte differentiation antigen CD14PRO_0000020883Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi26 ↔ 37By similarity
Disulfide bondi35 ↔ 52By similarity
Glycosylationi38 – 381N-linked (GlcNAc...)Sequence analysis
Glycosylationi150 – 1501N-linked (GlcNAc...)Sequence analysis
Disulfide bondi186 ↔ 216By similarity
Disulfide bondi240 ↔ 270By similarity
Glycosylationi280 – 2801N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PaxDbiQ95122.
PeptideAtlasiQ95122.
PRIDEiQ95122.

Expressioni

Tissue specificityi

Detected in lung (PubMed:9300371, PubMed:8643545). Detected in brain and kidney (PubMed:9300371). Detected in trachea and bone marrow (PubMed:8643545).2 Publications

Inductioni

Up-regulated in peripheral blood monocytes exposed to bacterial lipopolysaccharide (LPS).1 Publication

Interactioni

Subunit structurei

Belongs to the lipopolysaccharide (LPS) receptor, a multi-protein complex containing at least CD14, LY96 and TLR4 (By similarity). Interacts with LPS-bound LPB. Interacts with LPAR1 (By similarity). Interacts with the TLR2:TLR6 or TLR2:TLR1 heterodimers; upon interaction with ligands such as diacylated lipopeptides and triacylated lipopeptides, respectively (By similarity).By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000020009.

Structurei

3D structure databases

ProteinModelPortaliQ95122.
SMRiQ95122. Positions 24-331.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati55 – 8127LRR 1Add
BLAST
Repeati82 – 11736LRR 2Add
BLAST
Repeati118 – 14326LRR 3Add
BLAST
Repeati144 – 17128LRR 4Add
BLAST
Repeati172 – 19524LRR 5Add
BLAST
Repeati196 – 22328LRR 6Add
BLAST
Repeati224 – 25027LRR 7Add
BLAST
Repeati251 – 27626LRR 8Add
BLAST
Repeati277 – 29721LRR 9Add
BLAST
Repeati298 – 31922LRR 10Add
BLAST
Repeati320 – 34728LRR 11Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni288 – 37386Required for response to bacterial lipopolysaccharide (LPS)By similarityAdd
BLAST

Domaini

The C-terminal leucine-rich repeat (LRR) region is required for responses to smooth LPS.By similarity

Sequence similaritiesi

Contains 11 LRR (leucine-rich) repeats.Curated

Keywords - Domaini

Leucine-rich repeat, Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IXG9. Eukaryota.
ENOG41118N2. LUCA.
HOVERGENiHBG005269.
InParanoidiQ95122.
KOiK04391.

Family and domain databases

Gene3Di3.80.10.10. 1 hit.
InterProiIPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR016337. Monocyte_diff_Ag_CD14.
[Graphical view]
PfamiPF13516. LRR_6. 1 hit.
[Graphical view]
PIRSFiPIRSF002017. CD14. 1 hit.
PROSITEiPS51450. LRR. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q95122-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVCVPYLLLL LLPSLLRVSA DTTEPCELDD DDFRCVCNFT DPKPDWSSAV
60 70 80 90 100
QCMVAVEVEI SAGGRSLEQF LKGADTNPKQ YADTIKALRV RRLKLGAAQV
110 120 130 140 150
PAQLLVAVLR ALGYSRLKEL TLEDLEVTGP TPPTPLEAAG PALTTLSLRN
160 170 180 190 200
VSWTTGGAWL GELQQWLKPG LRVLNIAQAH SLAFPCAGLS TFEALTTLDL
210 220 230 240 250
SDNPSLGDSG LMAALCPNKF PALQYLALRN AGMETPSGVC AALAAARVQP
260 270 280 290 300
QSLDLSHNSL RVTAPGATRC VWPSALRSLN LSFAGLEQVP KGLPPKLSVL
310 320 330 340 350
DLSCNKLSRE PRRDELPEVN DLTLDGNPFL DPGALQHQND PMISGVVPAC
360 370
ARSALTMGVS GALALLQGAR GFA
Length:373
Mass (Da):39,667
Last modified:December 15, 1998 - v2
Checksum:iB9E9C2C51BE82227
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti209 – 2091S → T in AAD32215 (Ref. 2) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D84509 Genomic DNA. Translation: BAA21517.1.
AF141313 mRNA. Translation: AAD32215.1.
EU148609 Genomic DNA. Translation: ABV68569.1.
EU148610 Genomic DNA. Translation: ABV68570.1.
U48356 mRNA. Translation: AAB07861.1.
RefSeqiNP_776433.1. NM_174008.1.
UniGeneiBt.4285.

Genome annotation databases

GeneIDi281048.
KEGGibta:281048.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D84509 Genomic DNA. Translation: BAA21517.1.
AF141313 mRNA. Translation: AAD32215.1.
EU148609 Genomic DNA. Translation: ABV68569.1.
EU148610 Genomic DNA. Translation: ABV68570.1.
U48356 mRNA. Translation: AAB07861.1.
RefSeqiNP_776433.1. NM_174008.1.
UniGeneiBt.4285.

3D structure databases

ProteinModelPortaliQ95122.
SMRiQ95122. Positions 24-331.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000020009.

Proteomic databases

PaxDbiQ95122.
PeptideAtlasiQ95122.
PRIDEiQ95122.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi281048.
KEGGibta:281048.

Organism-specific databases

CTDi929.

Phylogenomic databases

eggNOGiENOG410IXG9. Eukaryota.
ENOG41118N2. LUCA.
HOVERGENiHBG005269.
InParanoidiQ95122.
KOiK04391.

Family and domain databases

Gene3Di3.80.10.10. 1 hit.
InterProiIPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR016337. Monocyte_diff_Ag_CD14.
[Graphical view]
PfamiPF13516. LRR_6. 1 hit.
[Graphical view]
PIRSFiPIRSF002017. CD14. 1 hit.
PROSITEiPS51450. LRR. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY, INDUCTION BY BACTERIAL LIPOPOLYSACCHARIDE.
    Strain: Holstein.
  2. "Cloning of cDNA for bovine CD14."
    Filipp D., Julius M.J.
    Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Holstein-Friesian.
  3. "Genetic mutations in the CD14 gene of cattle and relationship with the percentage of surface CD14-bound monocytes and neutrophils in healthy dairy cows."
    Ibeagha-Awemu E.M., Lee J.-W., Ibeagha A.E., Zhao X.
    Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "Inducible expression of an antibiotic peptide gene in lipopolysaccharide-challenged tracheal epithelial cells."
    Diamond G., Russell J.P., Bevins C.L.
    Proc. Natl. Acad. Sci. U.S.A. 93:5156-5160(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 179-278, TISSUE SPECIFICITY.

Entry informationi

Entry nameiCD14_BOVIN
AccessioniPrimary (citable) accession number: Q95122
Secondary accession number(s): A8DBS7, Q9TVA7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: December 15, 1998
Last modified: July 6, 2016
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.