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Protein

Monocyte differentiation antigen CD14

Gene

CD14

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Coreceptor for bacterial lipopolysaccharide. In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway. Binds electronegative LDL (LDL-) and mediates the cytokine release induced by LDL- (By similarity).By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Immunity, Inflammatory response, Innate immunity

Names & Taxonomyi

Protein namesi
Recommended name:
Monocyte differentiation antigen CD14
Alternative name(s):
Myeloid cell-specific leucine-rich glycoprotein
CD_antigen: CD14
Gene namesi
Name:CD14
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

  • Cell membrane By similarity; Lipid-anchorGPI-anchor By similarity
  • Secreted By similarity
  • Membrane raft By similarity
  • Golgi apparatus By similarity

  • Note: Soluble, secreted forms seem to exist. They may arise by cleavage of the GPI anchor.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Golgi apparatus, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_000002088321 – 373Monocyte differentiation antigen CD14Add BLAST353

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi26 ↔ 37By similarity
Disulfide bondi35 ↔ 52By similarity
Glycosylationi38N-linked (GlcNAc...)Sequence analysis1
Glycosylationi150N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi186 ↔ 216By similarity
Disulfide bondi240 ↔ 270By similarity
Glycosylationi280N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PaxDbiQ95122.
PeptideAtlasiQ95122.
PRIDEiQ95122.

Expressioni

Tissue specificityi

Detected in lung (PubMed:9300371, PubMed:8643545). Detected in brain and kidney (PubMed:9300371). Detected in trachea and bone marrow (PubMed:8643545).2 Publications

Inductioni

Up-regulated in peripheral blood monocytes exposed to bacterial lipopolysaccharide (LPS).1 Publication

Interactioni

Subunit structurei

Belongs to the lipopolysaccharide (LPS) receptor, a multi-protein complex containing at least CD14, LY96 and TLR4. Interacts with LPS-bound LPB. Interacts with LPAR1. Interacts with the TLR2:TLR6 or TLR2:TLR1 heterodimers; upon interaction with ligands such as diacylated lipopeptides and triacylated lipopeptides, respectively. Interacts with MYO18A.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000020009.

Structurei

3D structure databases

ProteinModelPortaliQ95122.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati55 – 81LRR 1Add BLAST27
Repeati82 – 117LRR 2Add BLAST36
Repeati118 – 143LRR 3Add BLAST26
Repeati144 – 171LRR 4Add BLAST28
Repeati172 – 195LRR 5Add BLAST24
Repeati196 – 223LRR 6Add BLAST28
Repeati224 – 250LRR 7Add BLAST27
Repeati251 – 276LRR 8Add BLAST26
Repeati277 – 297LRR 9Add BLAST21
Repeati298 – 319LRR 10Add BLAST22
Repeati320 – 347LRR 11Add BLAST28

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni288 – 373Required for response to bacterial lipopolysaccharide (LPS)By similarityAdd BLAST86

Domaini

The C-terminal leucine-rich repeat (LRR) region is required for responses to smooth LPS.By similarity

Sequence similaritiesi

Contains 11 LRR (leucine-rich) repeats.Curated

Keywords - Domaini

Leucine-rich repeat, Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IXG9. Eukaryota.
ENOG41118N2. LUCA.
HOVERGENiHBG005269.
InParanoidiQ95122.
KOiK04391.

Family and domain databases

Gene3Di3.80.10.10. 1 hit.
InterProiIPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR016337. Monocyte_diff_Ag_CD14.
[Graphical view]
PfamiPF13516. LRR_6. 1 hit.
[Graphical view]
PIRSFiPIRSF002017. CD14. 1 hit.
PROSITEiPS51450. LRR. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q95122-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVCVPYLLLL LLPSLLRVSA DTTEPCELDD DDFRCVCNFT DPKPDWSSAV
60 70 80 90 100
QCMVAVEVEI SAGGRSLEQF LKGADTNPKQ YADTIKALRV RRLKLGAAQV
110 120 130 140 150
PAQLLVAVLR ALGYSRLKEL TLEDLEVTGP TPPTPLEAAG PALTTLSLRN
160 170 180 190 200
VSWTTGGAWL GELQQWLKPG LRVLNIAQAH SLAFPCAGLS TFEALTTLDL
210 220 230 240 250
SDNPSLGDSG LMAALCPNKF PALQYLALRN AGMETPSGVC AALAAARVQP
260 270 280 290 300
QSLDLSHNSL RVTAPGATRC VWPSALRSLN LSFAGLEQVP KGLPPKLSVL
310 320 330 340 350
DLSCNKLSRE PRRDELPEVN DLTLDGNPFL DPGALQHQND PMISGVVPAC
360 370
ARSALTMGVS GALALLQGAR GFA
Length:373
Mass (Da):39,667
Last modified:December 15, 1998 - v2
Checksum:iB9E9C2C51BE82227
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti209S → T in AAD32215 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D84509 Genomic DNA. Translation: BAA21517.1.
AF141313 mRNA. Translation: AAD32215.1.
EU148609 Genomic DNA. Translation: ABV68569.1.
EU148610 Genomic DNA. Translation: ABV68570.1.
U48356 mRNA. Translation: AAB07861.1.
RefSeqiNP_776433.1. NM_174008.1.
UniGeneiBt.4285.

Genome annotation databases

GeneIDi281048.
KEGGibta:281048.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D84509 Genomic DNA. Translation: BAA21517.1.
AF141313 mRNA. Translation: AAD32215.1.
EU148609 Genomic DNA. Translation: ABV68569.1.
EU148610 Genomic DNA. Translation: ABV68570.1.
U48356 mRNA. Translation: AAB07861.1.
RefSeqiNP_776433.1. NM_174008.1.
UniGeneiBt.4285.

3D structure databases

ProteinModelPortaliQ95122.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000020009.

Proteomic databases

PaxDbiQ95122.
PeptideAtlasiQ95122.
PRIDEiQ95122.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi281048.
KEGGibta:281048.

Organism-specific databases

CTDi929.

Phylogenomic databases

eggNOGiENOG410IXG9. Eukaryota.
ENOG41118N2. LUCA.
HOVERGENiHBG005269.
InParanoidiQ95122.
KOiK04391.

Family and domain databases

Gene3Di3.80.10.10. 1 hit.
InterProiIPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR016337. Monocyte_diff_Ag_CD14.
[Graphical view]
PfamiPF13516. LRR_6. 1 hit.
[Graphical view]
PIRSFiPIRSF002017. CD14. 1 hit.
PROSITEiPS51450. LRR. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCD14_BOVIN
AccessioniPrimary (citable) accession number: Q95122
Secondary accession number(s): A8DBS7, Q9TVA7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: December 15, 1998
Last modified: November 30, 2016
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.