Q95117 (SFRP3_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Secreted frizzled-related protein 3
Short name=sFRP-3

Alternative name(s):
Frizzled-related protein 1
FrzB-1

Gene names

Name:	FRZB
Synonyms:	FRZB1, SFRP3

Organism

Bos taurus (Bovine) [Reference proteome]

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	325 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP3/FRZB appears to be involved in limb skeletogenesis. Antagonist of Wnt8 signaling. Regulates chondrocyte maturation and long bone development.
Subcellular location	Secreted Probable.
Domain	The FZ domain is involved in binding with Wnt ligands. Ref.3
Sequence similarities	Belongs to the secreted frizzled-related protein (sFRP) family. Contains 1 FZ (frizzled) domain. Contains 1 NTR domain.

Ontologies

Keywords
Biological process	Differentiation Wnt signaling pathway
Cellular component	Secreted
Domain	Signal
Molecular function	Developmental protein
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Wnt receptor signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW brain development Inferred from Biological aspect of Ancestor. Source: RefGenome cochlea morphogenesis Inferred from Biological aspect of Ancestor. Source: RefGenome convergent extension involved in organogenesis Inferred from electronic annotation. Source: Compara epithelium development Inferred from Biological aspect of Ancestor. Source: RefGenome gonad development Inferred from Biological aspect of Ancestor. Source: RefGenome mammary gland involution Inferred from Biological aspect of Ancestor. Source: RefGenome negative regulation of canonical Wnt receptor signaling pathway Inferred from Biological aspect of Ancestor. Source: RefGenome negative regulation of cartilage development Inferred from Biological aspect of Ancestor. Source: RefGenome negative regulation of cell development Inferred from electronic annotation. Source: Compara negative regulation of cell growth Inferred from electronic annotation. Source: Compara negative regulation of cell proliferation Inferred from electronic annotation. Source: Compara negative regulation of hepatocyte differentiation Inferred from Biological aspect of Ancestor. Source: RefGenome neural crest cell differentiation Inferred from electronic annotation. Source: Compara positive regulation of apoptotic process Inferred from electronic annotation. Source: Compara positive regulation of fat cell differentiation Inferred from electronic annotation. Source: Compara somite development Inferred from electronic annotation. Source: Compara vasculature development Inferred from Biological aspect of Ancestor. Source: RefGenome
Cellular_component	cytoplasm Inferred from Biological aspect of Ancestor. Source: RefGenome extracellular space Inferred from Biological aspect of Ancestor. Source: RefGenome
Molecular_function	PDZ domain binding Inferred from Biological aspect of Ancestor. Source: RefGenome Wnt-activated receptor activity Inferred from Biological aspect of Ancestor. Source: RefGenome Wnt-protein binding Inferred from sequence or structural similarity. Source: AgBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 32	32	Potential
	Chain	33 – 325	293	Secreted frizzled-related protein 3		PRO_0000032545
Regions
	Domain	33 – 150	118	FZ
	Domain	178 – 298	121	NTR
	Compositional bias	301 – 318	18	Ser-rich
Amino acid modifications
	Glycosylation	49	1	N-linked (GlcNAc...) Potential
	Glycosylation	299	1	N-linked (GlcNAc...) Potential
	Disulfide bond	35 ↔ 96		By similarity
	Disulfide bond	43 ↔ 89		By similarity
	Disulfide bond	80 ↔ 119		By similarity
	Disulfide bond	108 ↔ 147		By similarity
	Disulfide bond	112 ↔ 136		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q95117 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 39E337A9C6E98BB3	FASTA	325	36,234
	10 20 30 40 50 60 MVCGSRGGML LLPAGLLALA ALCLLRVPGA RAAACEPVRI PLCKSLPWNM TKMPNHLHHS 70 80 90 100 110 120 TQANAILAIE QFEGLLGTHC SPDLLFFLCA MYAPICTIDF QHEPIKPCKS VCERARQGCE 130 140 150 160 170 180 PILIKYRHSW PESLACEELP VYDRGVCISP EAIVTADGAD FPMDSSNGNC RGASSERCKC 190 200 210 220 230 240 KPVRATQKTY FRNNYNYVIR AKVKEIKTKC HDVTAVVEVK EILKASLVNI PRETVNLYTS 250 260 270 280 290 300 SGCLCPPLNV NEEYLIMGYE DEERSRLLLV EGSIAEKWKD RLGKKVKRWD MKLRHLGLNT 310 320 SDSSHSDSTQ SQKPGRNSNS RQARN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Primary structure and tissue distribution of FRZB, a novel protein related to Drosophila frizzled, suggest a role in skeletal morphogenesis." Hoang B., Moos M. Jr., Vukicevic S., Luyten F.P. J. Biol. Chem. 271:26131-26137(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Cartilage.
[2]	NIH - Mammalian Gene Collection (MGC) project Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Kidney.
[3]	"The cysteine-rich frizzled domain of Frzb-1 is required and sufficient for modulation of Wnt signaling." Lin K., Wang S., Julius M.A., Kitajewski J., Moos M. Jr., Luyten F.P. Proc. Natl. Acad. Sci. U.S.A. 94:11196-11200(1997) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION OF THE FZ DOMAIN WITH WNT PROTEINS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U24164 mRNA. Translation: AAC48662.1. BC112842 mRNA. Translation: AAI12843.1.
IPI	IPI00685423.
RefSeq	NP_776484.1. NM_174059.2.
UniGene	Bt.121.
3D structure databases
ProteinModelPortal	Q95117.
SMR	Q95117. Positions 33-157.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000014572; ENSBTAP00000014572; ENSBTAG00000010977.
GeneID	281170.
KEGG	bta:281170.
Organism-specific databases
CTD	2487.
Phylogenomic databases
eggNOG	NOG304384.
GeneTree	ENSGT00560000076776.
HOGENOM	HOG000231879.
HOVERGEN	HBG070536.
InParanoid	Q95117.
OMA	EEYLIMG.
OrthoDB	EOG4B8JDD.
Family and domain databases
Gene3D	1.10.2000.10. 1 hit.
InterPro	IPR015526. Frizzled/SFRP. IPR020067. Frizzled_dom. IPR001134. Netrin_domain. IPR018933. Netrin_module_non-TIMP. IPR026556. SFRP3. IPR008993. TIMP-like_OB-fold. [Graphical view]
PANTHER	PTHR11309. PTHR11309. 1 hit. PTHR11309:SF8. PTHR11309:SF8. 1 hit.
Pfam	PF01392. Fz. 1 hit. PF01759. NTR. 1 hit. [Graphical view]
SMART	SM00643. C345C. 1 hit. SM00063. FRI. 1 hit. [Graphical view]
SUPFAM	SSF63501. Frizzled_Cys-rich. 1 hit. SSF50242. TIMP_like. 1 hit.
PROSITE	PS50038. FZ. 1 hit. PS50189. NTR. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20805230.

Entry information

Entry name

SFRP3_BOVIN

Accession

Primary (citable) accession number: Q95117
Secondary accession number(s): Q2KHY0

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 2001
Last sequence update:	February 1, 1997
Last modified:	April 3, 2013
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program