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Q95117 (SFRP3_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Secreted frizzled-related protein 3

Short name=sFRP-3
Alternative name(s):
Frizzled-related protein 1
FrzB-1
Gene names
Name:FRZB
Synonyms:FRZB1, SFRP3
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length325 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP3/FRZB appears to be involved in limb skeletogenesis. Antagonist of Wnt8 signaling. Regulates chondrocyte maturation and long bone development.

Subunit structure

Interacts with MYOC By similarity. Ref.3

Subcellular location

Secreted Probable.

Domain

The FZ domain is involved in binding with Wnt ligands. Ref.3

Sequence similarities

Belongs to the secreted frizzled-related protein (sFRP) family.

Contains 1 FZ (frizzled) domain.

Contains 1 NTR domain.

Ontologies

Keywords
   Biological processDifferentiation
Wnt signaling pathway
   Cellular componentSecreted
   DomainSignal
   Molecular functionDevelopmental protein
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processWnt signaling pathway

Inferred from electronic annotation. Source: UniProtKB-KW

brain development

Inferred from Biological aspect of Ancestor. Source: RefGenome

cochlea morphogenesis

Inferred from Biological aspect of Ancestor. Source: RefGenome

convergent extension involved in organogenesis

Inferred from electronic annotation. Source: Ensembl

epithelium development

Inferred from Biological aspect of Ancestor. Source: RefGenome

gonad development

Inferred from Biological aspect of Ancestor. Source: RefGenome

mammary gland involution

Inferred from Biological aspect of Ancestor. Source: RefGenome

negative regulation of Wnt signaling pathway

Inferred from sequence or structural similarity. Source: AgBase

negative regulation of canonical Wnt signaling pathway

Inferred from Biological aspect of Ancestor. Source: RefGenome

negative regulation of cartilage development

Inferred from Biological aspect of Ancestor. Source: RefGenome

negative regulation of cell development

Inferred from electronic annotation. Source: Ensembl

negative regulation of cell growth

Inferred from electronic annotation. Source: Ensembl

negative regulation of cell proliferation

Inferred from electronic annotation. Source: Ensembl

negative regulation of hepatocyte differentiation

Inferred from Biological aspect of Ancestor. Source: RefGenome

neural crest cell differentiation

Inferred from electronic annotation. Source: Ensembl

positive regulation of apoptotic process

Inferred from electronic annotation. Source: Ensembl

positive regulation of fat cell differentiation

Inferred from electronic annotation. Source: Ensembl

somite development

Inferred from electronic annotation. Source: Ensembl

vasculature development

Inferred from Biological aspect of Ancestor. Source: RefGenome

   Cellular_componentcytoplasm

Inferred from Biological aspect of Ancestor. Source: RefGenome

extracellular space

Inferred from Biological aspect of Ancestor. Source: RefGenome

   Molecular_functionPDZ domain binding

Inferred from Biological aspect of Ancestor. Source: RefGenome

Wnt-activated receptor activity

Inferred from Biological aspect of Ancestor. Source: RefGenome

Wnt-protein binding

Inferred from sequence or structural similarity. Source: AgBase

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3232 Potential
Chain33 – 325293Secreted frizzled-related protein 3
PRO_0000032545

Regions

Domain33 – 150118FZ
Domain178 – 298121NTR
Compositional bias301 – 31818Ser-rich

Amino acid modifications

Glycosylation491N-linked (GlcNAc...) Potential
Glycosylation2991N-linked (GlcNAc...) Potential
Disulfide bond35 ↔ 96 By similarity
Disulfide bond43 ↔ 89 By similarity
Disulfide bond80 ↔ 119 By similarity
Disulfide bond108 ↔ 147 By similarity
Disulfide bond112 ↔ 136 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q95117 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: 39E337A9C6E98BB3

FASTA32536,234
        10         20         30         40         50         60 
MVCGSRGGML LLPAGLLALA ALCLLRVPGA RAAACEPVRI PLCKSLPWNM TKMPNHLHHS 

        70         80         90        100        110        120 
TQANAILAIE QFEGLLGTHC SPDLLFFLCA MYAPICTIDF QHEPIKPCKS VCERARQGCE 

       130        140        150        160        170        180 
PILIKYRHSW PESLACEELP VYDRGVCISP EAIVTADGAD FPMDSSNGNC RGASSERCKC 

       190        200        210        220        230        240 
KPVRATQKTY FRNNYNYVIR AKVKEIKTKC HDVTAVVEVK EILKASLVNI PRETVNLYTS 

       250        260        270        280        290        300 
SGCLCPPLNV NEEYLIMGYE DEERSRLLLV EGSIAEKWKD RLGKKVKRWD MKLRHLGLNT 

       310        320 
SDSSHSDSTQ SQKPGRNSNS RQARN 

« Hide

References

« Hide 'large scale' references
[1]"Primary structure and tissue distribution of FRZB, a novel protein related to Drosophila frizzled, suggest a role in skeletal morphogenesis."
Hoang B., Moos M. Jr., Vukicevic S., Luyten F.P.
J. Biol. Chem. 271:26131-26137(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Cartilage.
[2]NIH - Mammalian Gene Collection (MGC) project
Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Hereford.
Tissue: Kidney.
[3]"The cysteine-rich frizzled domain of Frzb-1 is required and sufficient for modulation of Wnt signaling."
Lin K., Wang S., Julius M.A., Kitajewski J., Moos M. Jr., Luyten F.P.
Proc. Natl. Acad. Sci. U.S.A. 94:11196-11200(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION OF THE FZ DOMAIN WITH WNT PROTEINS.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U24164 mRNA. Translation: AAC48662.1.
BC112842 mRNA. Translation: AAI12843.1.
RefSeqNP_776484.1. NM_174059.2.
XP_005202312.1. XM_005202255.1.
XP_005202313.1. XM_005202256.1.
UniGeneBt.121.

3D structure databases

ProteinModelPortalQ95117.
SMRQ95117. Positions 33-157.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSBTAT00000014572; ENSBTAP00000014572; ENSBTAG00000010977.
GeneID281170.
KEGGbta:281170.

Organism-specific databases

CTD2487.

Phylogenomic databases

eggNOGNOG304384.
GeneTreeENSGT00750000117492.
HOGENOMHOG000231879.
HOVERGENHBG070536.
InParanoidQ95117.
OMAEEYLIMG.
OrthoDBEOG79PJPC.

Family and domain databases

Gene3D1.10.2000.10. 1 hit.
InterProIPR015526. Frizzled/SFRP.
IPR020067. Frizzled_dom.
IPR001134. Netrin_domain.
IPR018933. Netrin_module_non-TIMP.
IPR026556. SFRP3.
IPR008993. TIMP-like_OB-fold.
[Graphical view]
PANTHERPTHR11309. PTHR11309. 1 hit.
PTHR11309:SF76. PTHR11309:SF76. 1 hit.
PfamPF01392. Fz. 1 hit.
PF01759. NTR. 1 hit.
[Graphical view]
SMARTSM00643. C345C. 1 hit.
SM00063. FRI. 1 hit.
[Graphical view]
SUPFAMSSF50242. SSF50242. 1 hit.
SSF63501. SSF63501. 1 hit.
PROSITEPS50038. FZ. 1 hit.
PS50189. NTR. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20805230.

Entry information

Entry nameSFRP3_BOVIN
AccessionPrimary (citable) accession number: Q95117
Secondary accession number(s): Q2KHY0
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: February 1, 1997
Last modified: June 11, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families