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Q95116 (TSP2_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Thrombospondin-2
Alternative name(s):
Corticotropin-induced secreted protein
Short name=CISP
Gene names
Name:THBS2
Synonyms:TSP-2, TSP2
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length1170 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Ligand for CD36 mediating antiangiogenic properties By similarity.

Subunit structure

Homotrimer; disulfide-linked. Can bind to fibrinogen, fibronectin, laminin and type V collagen. Interacts (via the TSP type I repeats) with CD36; the interaction conveys an antiangiogenic effect. Interacts (via the TSP type I repeats) with HRG; the interaction blocks the antiangiogenic effect of THBS2 with CD36 By similarity.

Sequence similarities

Belongs to the thrombospondin family.

Contains 2 EGF-like domains.

Contains 1 laminin G-like domain.

Contains 1 TSP C-terminal (TSPC) domain.

Contains 3 TSP type-1 domains.

Contains 8 TSP type-3 repeats.

Contains 1 VWFC domain.

Ontologies

Keywords
   Biological processCell adhesion
   DomainEGF-like domain
Repeat
Signal
   LigandCalcium
Heparin-binding
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcell adhesion

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: InterPro

   Molecular_functioncalcium ion binding

Inferred from electronic annotation. Source: InterPro

heparin binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1818 Potential
Chain19 – 11701152Thrombospondin-2
PRO_0000035845

Regions

Domain19 – 215197Laminin G-like
Domain318 – 37558VWFC
Domain379 – 42951TSP type-1 1
Domain435 – 49056TSP type-1 2
Domain492 – 54756TSP type-1 3
Domain547 – 58741EGF-like 1
Domain646 – 69045EGF-like 2
Repeat691 – 72636TSP type-3 1
Repeat727 – 76236TSP type-3 2
Repeat763 – 78523TSP type-3 3
Repeat786 – 82136TSP type-3 4
Repeat822 – 84423TSP type-3 5
Repeat845 – 88238TSP type-3 6
Repeat883 – 91836TSP type-3 7
Repeat919 – 95436TSP type-3 8
Domain958 – 1170213TSP C-terminal
Region19 – 232214Heparin-binding Potential
Motif926 – 9283Cell attachment site Potential

Amino acid modifications

Glycosylation1511N-linked (GlcNAc...) Potential
Glycosylation3161N-linked (GlcNAc...) Potential
Glycosylation3301N-linked (GlcNAc...) Potential
Glycosylation4551N-linked (GlcNAc...) Potential
Glycosylation5821N-linked (GlcNAc...) Potential
Glycosylation7081N-linked (GlcNAc...) Potential
Glycosylation9361N-linked (GlcNAc...) Potential
Glycosylation10671N-linked (GlcNAc...) Potential
Disulfide bond266Interchain Probable
Disulfide bond270Interchain Probable
Disulfide bond391 ↔ 423 By similarity
Disulfide bond395 ↔ 428 By similarity
Disulfide bond406 ↔ 413 By similarity
Disulfide bond447 ↔ 484 By similarity
Disulfide bond451 ↔ 489 By similarity
Disulfide bond462 ↔ 474 By similarity
Disulfide bond504 ↔ 541 By similarity
Disulfide bond508 ↔ 546 By similarity
Disulfide bond519 ↔ 531 By similarity
Disulfide bond551 ↔ 562 By similarity
Disulfide bond556 ↔ 572 By similarity
Disulfide bond575 ↔ 586 By similarity
Disulfide bond592 ↔ 608 By similarity
Disulfide bond599 ↔ 617 By similarity
Disulfide bond620 ↔ 644 By similarity
Disulfide bond650 ↔ 663 By similarity
Disulfide bond657 ↔ 676 By similarity
Disulfide bond678 ↔ 689 By similarity
Disulfide bond705 ↔ 713 By similarity
Disulfide bond718 ↔ 738 By similarity
Disulfide bond754 ↔ 774 By similarity
Disulfide bond777 ↔ 797 By similarity
Disulfide bond813 ↔ 833 By similarity
Disulfide bond836 ↔ 856 By similarity
Disulfide bond874 ↔ 894 By similarity
Disulfide bond910 ↔ 930 By similarity
Disulfide bond946 ↔ 1167 By similarity

Experimental info

Sequence conflict5351A → V in CAA60952. Ref.3
Sequence conflict7481S → T in CAA60952. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q95116 [UniParc].

Last modified July 15, 1999. Version 2.
Checksum: 9CF1FBF55B89A051

FASTA1,170129,863
        10         20         30         40         50         60 
MLWPLLLLAL WAWPSAQAGD QDEDTAFDLF SISNINRKTI GAKQFRGPDP SVPAYRFVRF 

        70         80         90        100        110        120 
DYIPPVSAEH LGRITEAMRR KEGFFLTASM KQDRRSRGTL LALEGPGATH RQFEIVSNGP 

       130        140        150        160        170        180 
ADTLDLTYWV DGTQHVISLE DVGLADSQWK NVTVQVTGET YSLYVGCDLM DSFALDEPFY 

       190        200        210        220        230        240 
EHLQTERSRM YVTKGAARES HFRGLLQNVY LVFENSVEDL LSKKGCQQSQ GAETNAISEN 

       250        260        270        280        290        300 
TETLHLSPMV TMEHVGPSAE KSPEVCEHSC EELGSMIREL SGLHVIVNQL HENLRKVSND 

       310        320        330        340        350        360 
NQFLWELIGG PPKTRNVSAC WQDGRFFAEN ETWVVDSCTK CTCKKFKTVC HQISCPPATC 

       370        380        390        400        410        420 
ADPWFVEGEC CPSCVHDGEE GWSPWAEWTE CSATCGSGTQ QRGRSCDVTS NTCLGPSIQT 

       430        440        450        460        470        480 
RACSLGRCDH RIRQDGGWSH WSPWSSCSVT CGVGNVTRIR LCNSPVPQMG GRSCKGSGRE 

       490        500        510        520        530        540 
TKACQGPPCP VDGRWSPWSP WSACTVTCAG GIRERTRVCN SPEPQHGGKD CVGGAKEQQM 

       550        560        570        580        590        600 
CNRKSCPIDG CLSNPCFPGA ECSSFPDGSW SCGSCPGGFL GNGTHCEDLD ECAVVTDVCF 

       610        620        630        640        650        660 
ATSKAHRCVN TNPGYHCLPC PPRYKGNQPF GVGLEAARTE KQVCEPENPC KDKTHSCHRH 

       670        680        690        700        710        720 
AECIYLGHFS DPMYKCECQT GYAGDGLICG EDSDLDGWPN KNLVCATNAT YHCVKDNCPL 

       730        740        750        760        770        780 
LPNSGQEDFD KDGIGDACDD DDDNDGVSDE KDNCQLLFNP RQFDYDKDEV GDRCDNCPYV 

       790        800        810        820        830        840 
HNPAQIDTDN NGEGDACSVD IDGDDVFNER DNCPYVYNTD QRDTDGDGVG DHCDNCPLVH 

       850        860        870        880        890        900 
NPDQTDVDND LVGDQCDNNE DIDEDGHQNN QDNCPHIPNA NQADHDRDGQ GDACDSDDDN 

       910        920        930        940        950        960 
DGIPDDRDNC RLVANPDQED SDGDRRGDAC KDDFDNDSIP DIDDVCPENN AISETDFRNF 

       970        980        990       1000       1010       1020 
QMVHLDPKGT TQIDPNWVIR HQGKELVQTA NSDPGIAVGF DEFGSVDFSG TFYVNTDRDD 

      1030       1040       1050       1060       1070       1080 
DYAGFVFGYQ SSSRFYVVMW KQVTQTYWED QPTRAYGYSG VSLKVVNSTT GTGEHLRNAL 

      1090       1100       1110       1120       1130       1140 
WHTGNTEGQV RTLWHDPKNI GWKDYTAYRW HLTHRPKTGY IRVLVHEGKQ VMADSGPIYD 

      1150       1160       1170 
QTYAGGRLGL FVFSQEMVYF SDLKYECRDV

« Hide

References

[1]Danik M., Chinn A., Lafeuillade M., Keramidas M., Aguesse-Germon S., Penhoat A., Chen H., Mosher D., Chambaz E.M., Feige J.J.
Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Opposite regulation of thrombospondin-1 and corticotropin-induced secreted protein/thrombospondin-2 expression by adrenocorticotropic hormone in adrenocortical cells."
Lafeuillade B., Pellerin S., Keramidas M., Danik M., Chambaz E.M., Feige J.J.
J. Cell. Physiol. 167:164-172(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-522.
[3]"Cloning and sequencing of bovine thrombospondin stimulatory effect of TGF-beta."
Zafar R.S., Moll Y.D., Womack J.F., Walz D.A.
Submitted (MAY-1995) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 318-831.
Tissue: Aortic endothelium.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X96540 mRNA. Translation: CAA65385.1.
X87620 mRNA. Translation: CAA60952.1.
IPIIPI00700466.
RefSeqNP_789861.1. NM_176872.1.
UniGeneBt.5522.

3D structure databases

ProteinModelPortalQ95116.
SMRQ95116. Positions 549-1169.
ModBaseSearch...

Proteomic databases

PRIDEQ95116.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID338092.
KEGGbta:338092.

Organism-specific databases

CTD7058.

Phylogenomic databases

eggNOGNOG12793.
HOGENOMHOG000007542.
HOVERGENHBG018006.
InParanoidQ95116.
KOK04659.
OrthoDBEOG41JZBD.

Family and domain databases

Gene3D2.60.120.200. 3 hits.
InterProIPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR000742. EG-like_dom.
IPR001881. EGF-like_Ca-bd.
IPR013032. EGF-like_CS.
IPR024731. EGF_dom_MSP1-like.
IPR001791. Laminin_G.
IPR000884. Thrombospondin_1_rpt.
IPR015455. Thrombospondin_2.
IPR003367. Thrombospondin_3-like_rpt.
IPR017897. Thrombospondin_3_rpt.
IPR008859. Thrombospondin_C.
IPR001007. VWF_C.
[Graphical view]
PANTHERPTHR10199:SF10. PTHR10199:SF10. 1 hit.
PfamPF12947. EGF_3. 1 hit.
PF07645. EGF_CA. 1 hit.
PF00090. TSP_1. 3 hits.
PF02412. TSP_3. 7 hits.
PF05735. TSP_C. 1 hit.
PF00093. VWC. 1 hit.
[Graphical view]
SMARTSM00181. EGF. 3 hits.
SM00209. TSP1. 3 hits.
SM00210. TSPN. 1 hit.
SM00214. VWC. 1 hit.
[Graphical view]
SUPFAMSSF49899. ConA_like_lec_gl. 2 hits.
SSF82895. TSP1. 3 hits.
PROSITEPS00022. EGF_1. False negative.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 2 hits.
PS50025. LAM_G_DOMAIN. False negative.
PS50092. TSP1. 3 hits.
PS51234. TSP3. 8 hits.
PS51236. TSP_CTER. 1 hit.
PS01208. VWFC_1. 1 hit.
PS50184. VWFC_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20812537.

Entry information

Entry nameTSP2_BOVIN
AccessionPrimary (citable) accession number: Q95116
Secondary accession number(s): Q28180
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 15, 1999
Last modified: April 3, 2013
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents