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Protein

Neural Wiskott-Aldrich syndrome protein

Gene

WASL

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in mitosis and cytokinesis, via its role in the regulation of actin polymerization (By similarity). Regulates actin polymerization by stimulating the actin-nucleating activity of the actin-related protein 2/3 (Arp2/3) complex. Binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression.By similarity1 Publication

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis, Transcription, Transcription regulation

Keywords - Ligandi

Actin-binding

Enzyme and pathway databases

ReactomeiR-BTA-2029482. Regulation of actin dynamics for phagocytic cup formation.
R-BTA-203641. NOSTRIN mediated eNOS trafficking.
R-BTA-373753. Nephrin interactions.
R-BTA-3928662. EPHB-mediated forward signaling.
R-BTA-418885. DCC mediated attractive signaling.
R-BTA-5663213. RHO GTPases Activate WASPs and WAVEs.
R-BTA-8856828. Clathrin-mediated endocytosis.

Names & Taxonomyi

Protein namesi
Recommended name:
Neural Wiskott-Aldrich syndrome protein
Short name:
N-WASP
Gene namesi
Name:WASL
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 4

Subcellular locationi

  • Nucleus By similarity
  • Cytoplasm By similarity
  • Cytoplasmcytoskeleton By similarity

  • Note: Preferentially localized in the cytoplasm when phosphorylated and in the nucleus when unphosphorylated. Exported from the nucleus by an nuclear export signal (NES)-dependent mechanism to the cytoplasm.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001889992 – 505Neural Wiskott-Aldrich syndrome proteinAdd BLAST504

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei242Phosphoserine; by TNK2By similarity1
Modified residuei256Phosphotyrosine; by FAK1 and TNK2By similarity1
Modified residuei307Omega-N-methylarginineBy similarity1
Modified residuei484PhosphoserineBy similarity1
Modified residuei485PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

PaxDbiQ95107.
PeptideAtlasiQ95107.
PRIDEiQ95107.

PTM databases

iPTMnetiQ95107.

Expressioni

Gene expression databases

BgeeiENSBTAG00000004643.

Interactioni

Subunit structurei

Binds actin and the Arp2/3 complex. Interacts with CDC42. Binds to SH3 domains of GRB2. Interacts with the C-terminal SH3 domain of DNMBP. Interacts with the WW domains of PRPF40A/FBP11. Interacts with NOSTRIN. Binds to TNK2. Interacts with PTK2/FAK1. Interacts with PACSIN1, PACSIN2 and PACSIN3. Interacts with SNX33 (By similarity). Interacts with SNX9.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
itself3EBI-6162776,EBI-6162776
ACTR2A7MB622EBI-6162776,EBI-6162748
CttnQ605982EBI-6162776,EBI-397955From a different organism.

Protein-protein interaction databases

IntActiQ95107. 5 interactors.
STRINGi9913.ENSBTAP00000006094.

Structurei

3D structure databases

ProteinModelPortaliQ95107.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini34 – 141WH1PROSITE-ProRule annotationAdd BLAST108
Domaini203 – 216CRIBPROSITE-ProRule annotationAdd BLAST14
Domaini405 – 422WH2 1PROSITE-ProRule annotationAdd BLAST18
Domaini433 – 450WH2 2PROSITE-ProRule annotationAdd BLAST18

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi277 – 392Pro-richAdd BLAST116
Compositional biasi486 – 505Asp-richAdd BLAST20

Sequence similaritiesi

Contains 1 CRIB domain.PROSITE-ProRule annotation
Contains 1 WH1 domain.PROSITE-ProRule annotation
Contains 2 WH2 domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG3671. Eukaryota.
ENOG4111J0D. LUCA.
GeneTreeiENSGT00730000110895.
HOGENOMiHOG000143378.
HOVERGENiHBG000222.
InParanoidiQ95107.
KOiK05747.
OMAiWSKKCCG.
OrthoDBiEOG091G0VCD.
TreeFamiTF316736.

Family and domain databases

CDDicd01205. EVH1_WASP-like. 1 hit.
Gene3Di2.30.29.30. 1 hit.
3.90.810.10. 1 hit.
InterProiIPR000095. CRIB_dom.
IPR030214. N-WASP.
IPR011993. PH_dom-like.
IPR011026. WASP_C.
IPR033927. WASPfam_EVH1.
IPR000697. WH1/EVH1_dom.
IPR003124. WH2_dom.
[Graphical view]
PANTHERiPTHR23202:SF33. PTHR23202:SF33. 1 hit.
PfamiPF00786. PBD. 1 hit.
PF00568. WH1. 1 hit.
PF02205. WH2. 2 hits.
[Graphical view]
SMARTiSM00285. PBD. 1 hit.
SM00461. WH1. 1 hit.
SM00246. WH2. 2 hits.
[Graphical view]
SUPFAMiSSF47912. SSF47912. 2 hits.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50108. CRIB. 1 hit.
PS50229. WH1. 1 hit.
PS51082. WH2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q95107-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSGQQQPPP PRRVTNVGSL LLTPQENESL FTFLGKKCVT MSSAVVQLYA
60 70 80 90 100
ADRNCMWSKK CSGVACLVKD NPQRSYFLRI FDIKDGKLLW EQELYNNFVY
110 120 130 140 150
NSPRGYFHTF AGDTCQVALN FANEEEAKKF RKAVTDLLGR RQRKSEKRRD
160 170 180 190 200
PPNGPNLPMA TVDIKNPEIT TNRFYGPQIN NISHTKEKKK GKAKKKRLTK
210 220 230 240 250
ADIGTPSNFQ HIGHVGWDPN TGFDLNNLDP ELKNLFDMCG ISEAQLKDRE
260 270 280 290 300
TSKVIYDFIE KTGGVEAVKN ELRRQAPPPP PPSRGGPPPP PPPPHSSGPP
310 320 330 340 350
PPPARGRGAP PPPPSRAPTA APPPPPPSRP GVGAPPPPPN RMYPPPLPAL
360 370 380 390 400
PSSAPSGPPP PPPPLSVSGS VAPPPPPPPP PPPGPPPPPG LPSDGDHQVP
410 420 430 440 450
TPAGSKAALL DQIREGAQLK KVEQNSRPVS CSGRDALLDQ IRQGIQLKSV
460 470 480 490 500
TDAPESTPPA PAPTSGIVGA LMEVMQKRSK AIHSSDEDED EDDDEDFEDD

DEWED
Length:505
Mass (Da):54,672
Last modified:February 1, 1997 - v1
Checksum:i54B83B48F1CDB3B8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D67066 mRNA. Translation: BAA11082.1.
PIRiS72273.
RefSeqiNP_776644.1. NM_174219.2.
UniGeneiBt.359.

Genome annotation databases

EnsembliENSBTAT00000006094; ENSBTAP00000006094; ENSBTAG00000004643.
GeneIDi281577.
KEGGibta:281577.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D67066 mRNA. Translation: BAA11082.1.
PIRiS72273.
RefSeqiNP_776644.1. NM_174219.2.
UniGeneiBt.359.

3D structure databases

ProteinModelPortaliQ95107.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ95107. 5 interactors.
STRINGi9913.ENSBTAP00000006094.

PTM databases

iPTMnetiQ95107.

Proteomic databases

PaxDbiQ95107.
PeptideAtlasiQ95107.
PRIDEiQ95107.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000006094; ENSBTAP00000006094; ENSBTAG00000004643.
GeneIDi281577.
KEGGibta:281577.

Organism-specific databases

CTDi8976.

Phylogenomic databases

eggNOGiKOG3671. Eukaryota.
ENOG4111J0D. LUCA.
GeneTreeiENSGT00730000110895.
HOGENOMiHOG000143378.
HOVERGENiHBG000222.
InParanoidiQ95107.
KOiK05747.
OMAiWSKKCCG.
OrthoDBiEOG091G0VCD.
TreeFamiTF316736.

Enzyme and pathway databases

ReactomeiR-BTA-2029482. Regulation of actin dynamics for phagocytic cup formation.
R-BTA-203641. NOSTRIN mediated eNOS trafficking.
R-BTA-373753. Nephrin interactions.
R-BTA-3928662. EPHB-mediated forward signaling.
R-BTA-418885. DCC mediated attractive signaling.
R-BTA-5663213. RHO GTPases Activate WASPs and WAVEs.
R-BTA-8856828. Clathrin-mediated endocytosis.

Gene expression databases

BgeeiENSBTAG00000004643.

Family and domain databases

CDDicd01205. EVH1_WASP-like. 1 hit.
Gene3Di2.30.29.30. 1 hit.
3.90.810.10. 1 hit.
InterProiIPR000095. CRIB_dom.
IPR030214. N-WASP.
IPR011993. PH_dom-like.
IPR011026. WASP_C.
IPR033927. WASPfam_EVH1.
IPR000697. WH1/EVH1_dom.
IPR003124. WH2_dom.
[Graphical view]
PANTHERiPTHR23202:SF33. PTHR23202:SF33. 1 hit.
PfamiPF00786. PBD. 1 hit.
PF00568. WH1. 1 hit.
PF02205. WH2. 2 hits.
[Graphical view]
SMARTiSM00285. PBD. 1 hit.
SM00461. WH1. 1 hit.
SM00246. WH2. 2 hits.
[Graphical view]
SUPFAMiSSF47912. SSF47912. 2 hits.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50108. CRIB. 1 hit.
PS50229. WH1. 1 hit.
PS51082. WH2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiWASL_BOVIN
AccessioniPrimary (citable) accession number: Q95107
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: February 1, 1997
Last modified: November 30, 2016
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.