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Q95107 (WASL_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 111. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Neural Wiskott-Aldrich syndrome protein

Short name=N-WASP
Gene names
Name:WASL
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length505 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in mitosis and cytokinesis, via its role in the regulation of actin polymerization By similarity. Regulates actin polymerization by stimulating the actin-nucleating activity of the actin-related protein 2/3 (Arp2/3) complex. Binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression. Ref.2

Subunit structure

Binds actin and the Arp2/3 complex. Interacts with CDC42. Binds to SH3 domains of GRB2. Interacts with the C-terminal SH3 domain of DNMBP. Interacts with the WW domains of PRPF40A/FBP11. Interacts with NOSTRIN. Binds to TNK2. Interacts with PTK2/FAK1. Interacts with PACSIN1, PACSIN2 and PACSIN3. Interacts with SNX33 By similarity. Interacts with SNX9. Ref.2

Subcellular location

Cytoplasmcytoskeleton By similarity. Nucleus By similarity. Note: Preferentially localized in the cytoplasm when phosphorylated and in the nucleus when unphosphorylated By similarity.

Sequence similarities

Contains 1 CRIB domain.

Contains 1 WH1 domain.

Contains 2 WH2 domains.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

itself3EBI-6162776,EBI-6162776
ACTR2A7MB622EBI-6162776,EBI-6162748
CttnQ605982EBI-6162776,EBI-397955From a different organism.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 505504Neural Wiskott-Aldrich syndrome protein
PRO_0000188999

Regions

Domain34 – 141108WH1
Domain203 – 21614CRIB
Domain405 – 42218WH2 1
Domain433 – 45018WH2 2
Compositional bias277 – 392116Pro-rich
Compositional bias486 – 50520Asp-rich

Amino acid modifications

Modified residue21N-acetylserine By similarity
Modified residue2421Phosphoserine; by TNK2 By similarity
Modified residue2561Phosphotyrosine; by FAK1 and TNK2 By similarity
Modified residue4841Phosphoserine By similarity
Modified residue4851Phosphoserine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q95107 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: 54B83B48F1CDB3B8

FASTA50554,672
        10         20         30         40         50         60 
MSSGQQQPPP PRRVTNVGSL LLTPQENESL FTFLGKKCVT MSSAVVQLYA ADRNCMWSKK 

        70         80         90        100        110        120 
CSGVACLVKD NPQRSYFLRI FDIKDGKLLW EQELYNNFVY NSPRGYFHTF AGDTCQVALN 

       130        140        150        160        170        180 
FANEEEAKKF RKAVTDLLGR RQRKSEKRRD PPNGPNLPMA TVDIKNPEIT TNRFYGPQIN 

       190        200        210        220        230        240 
NISHTKEKKK GKAKKKRLTK ADIGTPSNFQ HIGHVGWDPN TGFDLNNLDP ELKNLFDMCG 

       250        260        270        280        290        300 
ISEAQLKDRE TSKVIYDFIE KTGGVEAVKN ELRRQAPPPP PPSRGGPPPP PPPPHSSGPP 

       310        320        330        340        350        360 
PPPARGRGAP PPPPSRAPTA APPPPPPSRP GVGAPPPPPN RMYPPPLPAL PSSAPSGPPP 

       370        380        390        400        410        420 
PPPPLSVSGS VAPPPPPPPP PPPGPPPPPG LPSDGDHQVP TPAGSKAALL DQIREGAQLK 

       430        440        450        460        470        480 
KVEQNSRPVS CSGRDALLDQ IRQGIQLKSV TDAPESTPPA PAPTSGIVGA LMEVMQKRSK 

       490        500 
AIHSSDEDED EDDDEDFEDD DEWED 

« Hide

References

[1]"N-WASP, a novel actin-depolymerizing protein, regulates the cortical cytoskeletal rearrangement in a PIP2-dependent manner downstream of tyrosine kinases."
Miki H., Miura K., Takenawa T.
EMBO J. 15:5326-5335(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[2]"SNX9 couples actin assembly to phosphoinositide signals and is required for membrane remodeling during endocytosis."
Yarar D., Waterman-Storer C.M., Schmid S.L.
Dev. Cell 13:43-56(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH SNX9.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D67066 mRNA. Translation: BAA11082.1.
PIRS72273.
RefSeqNP_776644.1. NM_174219.2.
UniGeneBt.359.

3D structure databases

ProteinModelPortalQ95107.
SMRQ95107. Positions 29-150, 204-275.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ95107. 5 interactions.
STRING9913.ENSBTAP00000006094.

Proteomic databases

PaxDbQ95107.
PRIDEQ95107.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSBTAT00000006094; ENSBTAP00000006094; ENSBTAG00000004643.
GeneID281577.
KEGGbta:281577.

Organism-specific databases

CTD8976.

Phylogenomic databases

eggNOGNOG270974.
GeneTreeENSGT00730000110895.
HOGENOMHOG000143378.
HOVERGENHBG000222.
InParanoidQ95107.
KOK05747.
OMASKKCCGV.
OrthoDBEOG7FJH20.
TreeFamTF316736.

Family and domain databases

Gene3D2.30.29.30. 1 hit.
3.90.810.10. 1 hit.
InterProIPR000095. CRIB_dom.
IPR011993. PH_like_dom.
IPR027641. WASP.
IPR011026. WASP_C.
IPR000697. WH1/EVH1.
IPR003124. WH2_dom.
[Graphical view]
PANTHERPTHR12779. PTHR12779. 1 hit.
PfamPF00786. PBD. 1 hit.
PF00568. WH1. 1 hit.
PF02205. WH2. 2 hits.
[Graphical view]
SMARTSM00285. PBD. 1 hit.
SM00461. WH1. 1 hit.
SM00246. WH2. 2 hits.
[Graphical view]
SUPFAMSSF47912. SSF47912. 2 hits.
PROSITEPS50108. CRIB. 1 hit.
PS50229. WH1. 1 hit.
PS51082. WH2. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio20805526.

Entry information

Entry nameWASL_BOVIN
AccessionPrimary (citable) accession number: Q95107
Entry history
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: February 1, 1997
Last modified: April 16, 2014
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families