Neural Wiskott-Aldrich syndrome protein

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Q95107 (WASL_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 102. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Neural Wiskott-Aldrich syndrome protein
Short name=N-WASP

Gene names

Name:

WASL

Organism

Bos taurus (Bovine) [Reference proteome]

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	505 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Regulates actin polymerization by stimulating the actin-nucleating activity of the actin-related protein 2/3 (Arp2/3) complex. Binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression. Ref.2
Subunit structure	Binds actin and the Arp2/3 complex. Interacts with CDC42. Binds to SH3 domains of GRB2. Interacts with the C-terminal SH3 domain of DNMBP. Interacts with the WW domains of PRPF40A/FBP11. Interacts with NOSTRIN. Binds to TNK2. Interacts with PTK2/FAK1 By similarity. Interacts with SNX9. Ref.2
Subcellular location	Cytoplasm › cytoskeleton By similarity. Nucleus By similarity. Note: Preferentially localized in the cytoplasm when phosphorylated and in the nucleus when unphosphorylated By similarity.
Sequence similarities	Contains 1 CRIB domain. Contains 1 WH1 domain. Contains 2 WH2 domains.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm Cytoskeleton Nucleus
Domain	Repeat
Ligand	Actin-binding
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	actin polymerization or depolymerization Inferred from electronic annotation. Source: InterPro cellular protein complex localization Inferred from electronic annotation. Source: Compara protein complex assembly Inferred from electronic annotation. Source: InterPro regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW response to bacterium Inferred from electronic annotation. Source: Compara spindle localization Inferred from electronic annotation. Source: Compara transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	actin cap Inferred from electronic annotation. Source: Compara lamellipodium Inferred from electronic annotation. Source: Compara nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	small GTPase regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		3	EBI-6162776,EBI-6162776
ACTR2	A7MB62	2	EBI-6162776,EBI-6162748
Cttn	Q60598	2	EBI-6162776,EBI-397955	From a different organism.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 505

505

Neural Wiskott-Aldrich syndrome protein

PRO_0000188999

Regions

Domain

34 – 141

108

WH1

Domain

203 – 216

CRIB

Domain

405 – 422

WH2 1

Domain

433 – 450

WH2 2

Compositional bias

277 – 392

116

Pro-rich

Compositional bias

486 – 505

Asp-rich

Amino acid modifications

Modified residue

242

Phosphoserine; by TNK2 By similarity

Modified residue

256

Phosphotyrosine; by FAK1 and TNK2 By similarity

Modified residue

484

Phosphoserine By similarity

Modified residue

485

Phosphoserine By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q95107 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: 54B83B48F1CDB3B8
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FASTA

505

54,672

        10         20         30         40         50         60 
MSSGQQQPPP PRRVTNVGSL LLTPQENESL FTFLGKKCVT MSSAVVQLYA ADRNCMWSKK 

        70         80         90        100        110        120 
CSGVACLVKD NPQRSYFLRI FDIKDGKLLW EQELYNNFVY NSPRGYFHTF AGDTCQVALN 

       130        140        150        160        170        180 
FANEEEAKKF RKAVTDLLGR RQRKSEKRRD PPNGPNLPMA TVDIKNPEIT TNRFYGPQIN 

       190        200        210        220        230        240 
NISHTKEKKK GKAKKKRLTK ADIGTPSNFQ HIGHVGWDPN TGFDLNNLDP ELKNLFDMCG 

       250        260        270        280        290        300 
ISEAQLKDRE TSKVIYDFIE KTGGVEAVKN ELRRQAPPPP PPSRGGPPPP PPPPHSSGPP 

       310        320        330        340        350        360 
PPPARGRGAP PPPPSRAPTA APPPPPPSRP GVGAPPPPPN RMYPPPLPAL PSSAPSGPPP 

       370        380        390        400        410        420 
PPPPLSVSGS VAPPPPPPPP PPPGPPPPPG LPSDGDHQVP TPAGSKAALL DQIREGAQLK 

       430        440        450        460        470        480 
KVEQNSRPVS CSGRDALLDQ IRQGIQLKSV TDAPESTPPA PAPTSGIVGA LMEVMQKRSK 

       490        500 
AIHSSDEDED EDDDEDFEDD DEWED

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References

[1]	"N-WASP, a novel actin-depolymerizing protein, regulates the cortical cytoskeletal rearrangement in a PIP2-dependent manner downstream of tyrosine kinases." Miki H., Miura K., Takenawa T. EMBO J. 15:5326-5335(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[2]	"SNX9 couples actin assembly to phosphoinositide signals and is required for membrane remodeling during endocytosis." Yarar D., Waterman-Storer C.M., Schmid S.L. Dev. Cell 13:43-56(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH SNX9.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D67066 mRNA. Translation: BAA11082.1.
IPI	IPI00713831.
PIR	S72273.
RefSeq	NP_776644.1. NM_174219.2.
UniGene	Bt.359.
3D structure databases
ProteinModelPortal	Q95107.
SMR	Q95107. Positions 29-150, 204-275.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q95107. 5 interactions.
STRING	9913.ENSBTAP00000006094.
Proteomic databases
PaxDb	Q95107.
PRIDE	Q95107.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000006094; ENSBTAP00000006094; ENSBTAG00000004643.
GeneID	281577.
KEGG	bta:281577.
Organism-specific databases
CTD	8976.
Phylogenomic databases
eggNOG	NOG270974.
GeneTree	ENSGT00550000074443.
HOGENOM	HOG000143378.
HOVERGEN	HBG000222.
InParanoid	Q95107.
KO	K05747.
OMA	SKKCCGV.
OrthoDB	EOG4JM7Q1.
Gene expression databases
ArrayExpress	Q95107.
Family and domain databases
Gene3D	2.30.29.30. 1 hit.
InterPro	IPR000697. EVH1. IPR000095. PAK_box_Rho-bd. IPR011993. PH_like_dom. IPR011026. WASP_C. IPR003124. WH2_dom. [Graphical view]
Pfam	PF00786. PBD. 1 hit. PF00568. WH1. 1 hit. PF02205. WH2. 2 hits. [Graphical view]
SMART	SM00285. PBD. 1 hit. SM00461. WH1. 1 hit. SM00246. WH2. 2 hits. [Graphical view]
SUPFAM	SSF47912. WASP_C. 2 hits.
PROSITE	PS50108. CRIB. 1 hit. PS50229. WH1. 1 hit. PS51082. WH2. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	20805526.

Entry information

Entry name

WASL_BOVIN

Accession

Primary (citable) accession number: Q95107

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	February 1, 1997
Last modified:	May 1, 2013
This is version 102 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents