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Q95107

- WASL_BOVIN

UniProt

Q95107 - WASL_BOVIN

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Protein

Neural Wiskott-Aldrich syndrome protein

Gene

WASL

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Involved in mitosis and cytokinesis, via its role in the regulation of actin polymerization (By similarity). Regulates actin polymerization by stimulating the actin-nucleating activity of the actin-related protein 2/3 (Arp2/3) complex. Binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression.By similarity1 Publication

GO - Molecular functioni

  1. identical protein binding Source: IntAct

GO - Biological processi

  1. actin filament organization Source: InterPro
  2. cellular protein complex localization Source: Ensembl
  3. mitotic nuclear division Source: UniProtKB-KW
  4. regulation of protein localization Source: Ensembl
  5. regulation of transcription, DNA-templated Source: UniProtKB-KW
  6. response to bacterium Source: Ensembl
  7. spindle localization Source: Ensembl
  8. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis, Transcription, Transcription regulation

Keywords - Ligandi

Actin-binding

Enzyme and pathway databases

ReactomeiREACT_211475. Regulation of actin dynamics for phagocytic cup formation.
REACT_211504. Nephrin interactions.
REACT_216118. DCC mediated attractive signaling.
REACT_226967. NOSTRIN mediated eNOS trafficking.
REACT_259381. EPHB-mediated forward signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
Neural Wiskott-Aldrich syndrome protein
Short name:
N-WASP
Gene namesi
Name:WASL
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 4

Subcellular locationi

Cytoplasmcytoskeleton By similarity. Nucleus By similarity
Note: Preferentially localized in the cytoplasm when phosphorylated and in the nucleus when unphosphorylated.By similarity

GO - Cellular componenti

  1. actin cap Source: Ensembl
  2. extracellular vesicular exosome Source: Ensembl
  3. lamellipodium Source: Ensembl
  4. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 505504Neural Wiskott-Aldrich syndrome proteinPRO_0000188999Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Modified residuei242 – 2421Phosphoserine; by TNK2By similarity
Modified residuei256 – 2561Phosphotyrosine; by FAK1 and TNK2By similarity
Modified residuei484 – 4841PhosphoserineBy similarity
Modified residuei485 – 4851PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ95107.
PRIDEiQ95107.

Expressioni

Gene expression databases

ExpressionAtlasiQ95107. baseline.

Interactioni

Subunit structurei

Binds actin and the Arp2/3 complex. Interacts with CDC42. Binds to SH3 domains of GRB2. Interacts with the C-terminal SH3 domain of DNMBP. Interacts with the WW domains of PRPF40A/FBP11. Interacts with NOSTRIN. Binds to TNK2. Interacts with PTK2/FAK1. Interacts with PACSIN1, PACSIN2 and PACSIN3. Interacts with SNX33 (By similarity). Interacts with SNX9.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
itself3EBI-6162776,EBI-6162776
ACTR2A7MB622EBI-6162776,EBI-6162748
CttnQ605982EBI-6162776,EBI-397955From a different organism.

Protein-protein interaction databases

IntActiQ95107. 5 interactions.
STRINGi9913.ENSBTAP00000006094.

Structurei

3D structure databases

ProteinModelPortaliQ95107.
SMRiQ95107. Positions 29-150, 204-275.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini34 – 141108WH1PROSITE-ProRule annotationAdd
BLAST
Domaini203 – 21614CRIBPROSITE-ProRule annotationAdd
BLAST
Domaini405 – 42218WH2 1PROSITE-ProRule annotationAdd
BLAST
Domaini433 – 45018WH2 2PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi277 – 392116Pro-richAdd
BLAST
Compositional biasi486 – 50520Asp-richAdd
BLAST

Sequence similaritiesi

Contains 1 CRIB domain.PROSITE-ProRule annotation
Contains 1 WH1 domain.PROSITE-ProRule annotation
Contains 2 WH2 domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG270974.
GeneTreeiENSGT00730000110895.
HOGENOMiHOG000143378.
HOVERGENiHBG000222.
InParanoidiQ95107.
KOiK05747.
OMAiPTPAGNK.
OrthoDBiEOG7FJH20.
TreeFamiTF316736.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
3.90.810.10. 1 hit.
InterProiIPR000095. CRIB_dom.
IPR011993. PH_like_dom.
IPR011026. WASP_C.
IPR000697. WH1/EVH1.
IPR003124. WH2_dom.
[Graphical view]
PfamiPF00786. PBD. 1 hit.
PF00568. WH1. 1 hit.
PF02205. WH2. 2 hits.
[Graphical view]
SMARTiSM00285. PBD. 1 hit.
SM00461. WH1. 1 hit.
SM00246. WH2. 2 hits.
[Graphical view]
SUPFAMiSSF47912. SSF47912. 2 hits.
PROSITEiPS50108. CRIB. 1 hit.
PS50229. WH1. 1 hit.
PS51082. WH2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q95107-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSSGQQQPPP PRRVTNVGSL LLTPQENESL FTFLGKKCVT MSSAVVQLYA
60 70 80 90 100
ADRNCMWSKK CSGVACLVKD NPQRSYFLRI FDIKDGKLLW EQELYNNFVY
110 120 130 140 150
NSPRGYFHTF AGDTCQVALN FANEEEAKKF RKAVTDLLGR RQRKSEKRRD
160 170 180 190 200
PPNGPNLPMA TVDIKNPEIT TNRFYGPQIN NISHTKEKKK GKAKKKRLTK
210 220 230 240 250
ADIGTPSNFQ HIGHVGWDPN TGFDLNNLDP ELKNLFDMCG ISEAQLKDRE
260 270 280 290 300
TSKVIYDFIE KTGGVEAVKN ELRRQAPPPP PPSRGGPPPP PPPPHSSGPP
310 320 330 340 350
PPPARGRGAP PPPPSRAPTA APPPPPPSRP GVGAPPPPPN RMYPPPLPAL
360 370 380 390 400
PSSAPSGPPP PPPPLSVSGS VAPPPPPPPP PPPGPPPPPG LPSDGDHQVP
410 420 430 440 450
TPAGSKAALL DQIREGAQLK KVEQNSRPVS CSGRDALLDQ IRQGIQLKSV
460 470 480 490 500
TDAPESTPPA PAPTSGIVGA LMEVMQKRSK AIHSSDEDED EDDDEDFEDD

DEWED
Length:505
Mass (Da):54,672
Last modified:February 1, 1997 - v1
Checksum:i54B83B48F1CDB3B8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D67066 mRNA. Translation: BAA11082.1.
PIRiS72273.
RefSeqiNP_776644.1. NM_174219.2.
UniGeneiBt.359.

Genome annotation databases

EnsembliENSBTAT00000006094; ENSBTAP00000006094; ENSBTAG00000004643.
GeneIDi281577.
KEGGibta:281577.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D67066 mRNA. Translation: BAA11082.1 .
PIRi S72273.
RefSeqi NP_776644.1. NM_174219.2.
UniGenei Bt.359.

3D structure databases

ProteinModelPortali Q95107.
SMRi Q95107. Positions 29-150, 204-275.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q95107. 5 interactions.
STRINGi 9913.ENSBTAP00000006094.

Proteomic databases

PaxDbi Q95107.
PRIDEi Q95107.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSBTAT00000006094 ; ENSBTAP00000006094 ; ENSBTAG00000004643 .
GeneIDi 281577.
KEGGi bta:281577.

Organism-specific databases

CTDi 8976.

Phylogenomic databases

eggNOGi NOG270974.
GeneTreei ENSGT00730000110895.
HOGENOMi HOG000143378.
HOVERGENi HBG000222.
InParanoidi Q95107.
KOi K05747.
OMAi PTPAGNK.
OrthoDBi EOG7FJH20.
TreeFami TF316736.

Enzyme and pathway databases

Reactomei REACT_211475. Regulation of actin dynamics for phagocytic cup formation.
REACT_211504. Nephrin interactions.
REACT_216118. DCC mediated attractive signaling.
REACT_226967. NOSTRIN mediated eNOS trafficking.
REACT_259381. EPHB-mediated forward signaling.

Miscellaneous databases

NextBioi 20805526.

Gene expression databases

ExpressionAtlasi Q95107. baseline.

Family and domain databases

Gene3Di 2.30.29.30. 1 hit.
3.90.810.10. 1 hit.
InterProi IPR000095. CRIB_dom.
IPR011993. PH_like_dom.
IPR011026. WASP_C.
IPR000697. WH1/EVH1.
IPR003124. WH2_dom.
[Graphical view ]
Pfami PF00786. PBD. 1 hit.
PF00568. WH1. 1 hit.
PF02205. WH2. 2 hits.
[Graphical view ]
SMARTi SM00285. PBD. 1 hit.
SM00461. WH1. 1 hit.
SM00246. WH2. 2 hits.
[Graphical view ]
SUPFAMi SSF47912. SSF47912. 2 hits.
PROSITEi PS50108. CRIB. 1 hit.
PS50229. WH1. 1 hit.
PS51082. WH2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "N-WASP, a novel actin-depolymerizing protein, regulates the cortical cytoskeletal rearrangement in a PIP2-dependent manner downstream of tyrosine kinases."
    Miki H., Miura K., Takenawa T.
    EMBO J. 15:5326-5335(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  2. "SNX9 couples actin assembly to phosphoinositide signals and is required for membrane remodeling during endocytosis."
    Yarar D., Waterman-Storer C.M., Schmid S.L.
    Dev. Cell 13:43-56(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH SNX9.

Entry informationi

Entry nameiWASL_BOVIN
AccessioniPrimary (citable) accession number: Q95107
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: February 1, 1997
Last modified: November 26, 2014
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3