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Q95107

- WASL_BOVIN

UniProt

Q95107 - WASL_BOVIN

Protein

Neural Wiskott-Aldrich syndrome protein

Gene

WASL

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 115 (01 Oct 2014)
      Sequence version 1 (01 Feb 1997)
      Previous versions | rss
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    Functioni

    Involved in mitosis and cytokinesis, via its role in the regulation of actin polymerization By similarity. Regulates actin polymerization by stimulating the actin-nucleating activity of the actin-related protein 2/3 (Arp2/3) complex. Binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression.By similarity1 Publication

    GO - Molecular functioni

    1. identical protein binding Source: IntAct
    2. protein binding Source: IntAct

    GO - Biological processi

    1. actin filament organization Source: InterPro
    2. cellular protein complex localization Source: Ensembl
    3. mitotic nuclear division Source: UniProtKB-KW
    4. regulation of protein localization Source: Ensembl
    5. regulation of transcription, DNA-templated Source: UniProtKB-KW
    6. response to bacterium Source: Ensembl
    7. spindle localization Source: Ensembl
    8. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis, Transcription, Transcription regulation

    Keywords - Ligandi

    Actin-binding

    Enzyme and pathway databases

    ReactomeiREACT_211475. Regulation of actin dynamics for phagocytic cup formation.
    REACT_211504. Nephrin interactions.
    REACT_216118. DCC mediated attractive signaling.
    REACT_226967. NOSTRIN mediated eNOS trafficking.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Neural Wiskott-Aldrich syndrome protein
    Short name:
    N-WASP
    Gene namesi
    Name:WASL
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 4

    Subcellular locationi

    Cytoplasmcytoskeleton By similarity. Nucleus By similarity
    Note: Preferentially localized in the cytoplasm when phosphorylated and in the nucleus when unphosphorylated.By similarity

    GO - Cellular componenti

    1. actin cap Source: Ensembl
    2. lamellipodium Source: Ensembl
    3. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 505504Neural Wiskott-Aldrich syndrome proteinPRO_0000188999Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserineBy similarity
    Modified residuei242 – 2421Phosphoserine; by TNK2By similarity
    Modified residuei256 – 2561Phosphotyrosine; by FAK1 and TNK2By similarity
    Modified residuei484 – 4841PhosphoserineBy similarity
    Modified residuei485 – 4851PhosphoserineBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PaxDbiQ95107.
    PRIDEiQ95107.

    Interactioni

    Subunit structurei

    Binds actin and the Arp2/3 complex. Interacts with CDC42. Binds to SH3 domains of GRB2. Interacts with the C-terminal SH3 domain of DNMBP. Interacts with the WW domains of PRPF40A/FBP11. Interacts with NOSTRIN. Binds to TNK2. Interacts with PTK2/FAK1. Interacts with PACSIN1, PACSIN2 and PACSIN3. Interacts with SNX33 By similarity. Interacts with SNX9.By similarity1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself3EBI-6162776,EBI-6162776
    ACTR2A7MB622EBI-6162776,EBI-6162748
    CttnQ605982EBI-6162776,EBI-397955From a different organism.

    Protein-protein interaction databases

    IntActiQ95107. 5 interactions.
    STRINGi9913.ENSBTAP00000006094.

    Structurei

    3D structure databases

    ProteinModelPortaliQ95107.
    SMRiQ95107. Positions 29-150, 204-275.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini34 – 141108WH1PROSITE-ProRule annotationAdd
    BLAST
    Domaini203 – 21614CRIBPROSITE-ProRule annotationAdd
    BLAST
    Domaini405 – 42218WH2 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini433 – 45018WH2 2PROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi277 – 392116Pro-richAdd
    BLAST
    Compositional biasi486 – 50520Asp-richAdd
    BLAST

    Sequence similaritiesi

    Contains 1 CRIB domain.PROSITE-ProRule annotation
    Contains 1 WH1 domain.PROSITE-ProRule annotation
    Contains 2 WH2 domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG270974.
    GeneTreeiENSGT00730000110895.
    HOGENOMiHOG000143378.
    HOVERGENiHBG000222.
    InParanoidiQ95107.
    KOiK05747.
    OMAiPTPAGNK.
    OrthoDBiEOG7FJH20.
    TreeFamiTF316736.

    Family and domain databases

    Gene3Di2.30.29.30. 1 hit.
    3.90.810.10. 1 hit.
    InterProiIPR000095. CRIB_dom.
    IPR011993. PH_like_dom.
    IPR011026. WASP_C.
    IPR000697. WH1/EVH1.
    IPR003124. WH2_dom.
    [Graphical view]
    PfamiPF00786. PBD. 1 hit.
    PF00568. WH1. 1 hit.
    PF02205. WH2. 2 hits.
    [Graphical view]
    SMARTiSM00285. PBD. 1 hit.
    SM00461. WH1. 1 hit.
    SM00246. WH2. 2 hits.
    [Graphical view]
    SUPFAMiSSF47912. SSF47912. 2 hits.
    PROSITEiPS50108. CRIB. 1 hit.
    PS50229. WH1. 1 hit.
    PS51082. WH2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q95107-1 [UniParc]FASTAAdd to Basket

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    MSSGQQQPPP PRRVTNVGSL LLTPQENESL FTFLGKKCVT MSSAVVQLYA    50
    ADRNCMWSKK CSGVACLVKD NPQRSYFLRI FDIKDGKLLW EQELYNNFVY 100
    NSPRGYFHTF AGDTCQVALN FANEEEAKKF RKAVTDLLGR RQRKSEKRRD 150
    PPNGPNLPMA TVDIKNPEIT TNRFYGPQIN NISHTKEKKK GKAKKKRLTK 200
    ADIGTPSNFQ HIGHVGWDPN TGFDLNNLDP ELKNLFDMCG ISEAQLKDRE 250
    TSKVIYDFIE KTGGVEAVKN ELRRQAPPPP PPSRGGPPPP PPPPHSSGPP 300
    PPPARGRGAP PPPPSRAPTA APPPPPPSRP GVGAPPPPPN RMYPPPLPAL 350
    PSSAPSGPPP PPPPLSVSGS VAPPPPPPPP PPPGPPPPPG LPSDGDHQVP 400
    TPAGSKAALL DQIREGAQLK KVEQNSRPVS CSGRDALLDQ IRQGIQLKSV 450
    TDAPESTPPA PAPTSGIVGA LMEVMQKRSK AIHSSDEDED EDDDEDFEDD 500
    DEWED 505
    Length:505
    Mass (Da):54,672
    Last modified:February 1, 1997 - v1
    Checksum:i54B83B48F1CDB3B8
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D67066 mRNA. Translation: BAA11082.1.
    PIRiS72273.
    RefSeqiNP_776644.1. NM_174219.2.
    UniGeneiBt.359.

    Genome annotation databases

    EnsembliENSBTAT00000006094; ENSBTAP00000006094; ENSBTAG00000004643.
    GeneIDi281577.
    KEGGibta:281577.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D67066 mRNA. Translation: BAA11082.1 .
    PIRi S72273.
    RefSeqi NP_776644.1. NM_174219.2.
    UniGenei Bt.359.

    3D structure databases

    ProteinModelPortali Q95107.
    SMRi Q95107. Positions 29-150, 204-275.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q95107. 5 interactions.
    STRINGi 9913.ENSBTAP00000006094.

    Proteomic databases

    PaxDbi Q95107.
    PRIDEi Q95107.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000006094 ; ENSBTAP00000006094 ; ENSBTAG00000004643 .
    GeneIDi 281577.
    KEGGi bta:281577.

    Organism-specific databases

    CTDi 8976.

    Phylogenomic databases

    eggNOGi NOG270974.
    GeneTreei ENSGT00730000110895.
    HOGENOMi HOG000143378.
    HOVERGENi HBG000222.
    InParanoidi Q95107.
    KOi K05747.
    OMAi PTPAGNK.
    OrthoDBi EOG7FJH20.
    TreeFami TF316736.

    Enzyme and pathway databases

    Reactomei REACT_211475. Regulation of actin dynamics for phagocytic cup formation.
    REACT_211504. Nephrin interactions.
    REACT_216118. DCC mediated attractive signaling.
    REACT_226967. NOSTRIN mediated eNOS trafficking.

    Miscellaneous databases

    NextBioi 20805526.

    Family and domain databases

    Gene3Di 2.30.29.30. 1 hit.
    3.90.810.10. 1 hit.
    InterProi IPR000095. CRIB_dom.
    IPR011993. PH_like_dom.
    IPR011026. WASP_C.
    IPR000697. WH1/EVH1.
    IPR003124. WH2_dom.
    [Graphical view ]
    Pfami PF00786. PBD. 1 hit.
    PF00568. WH1. 1 hit.
    PF02205. WH2. 2 hits.
    [Graphical view ]
    SMARTi SM00285. PBD. 1 hit.
    SM00461. WH1. 1 hit.
    SM00246. WH2. 2 hits.
    [Graphical view ]
    SUPFAMi SSF47912. SSF47912. 2 hits.
    PROSITEi PS50108. CRIB. 1 hit.
    PS50229. WH1. 1 hit.
    PS51082. WH2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "N-WASP, a novel actin-depolymerizing protein, regulates the cortical cytoskeletal rearrangement in a PIP2-dependent manner downstream of tyrosine kinases."
      Miki H., Miura K., Takenawa T.
      EMBO J. 15:5326-5335(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Brain.
    2. "SNX9 couples actin assembly to phosphoinositide signals and is required for membrane remodeling during endocytosis."
      Yarar D., Waterman-Storer C.M., Schmid S.L.
      Dev. Cell 13:43-56(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH SNX9.

    Entry informationi

    Entry nameiWASL_BOVIN
    AccessioniPrimary (citable) accession number: Q95107
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 27, 2001
    Last sequence update: February 1, 1997
    Last modified: October 1, 2014
    This is version 115 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3