Q95032 (METK_ACACA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
S-adenosylmethionine synthase
Short name=AdoMet synthase
EC=2.5.1.6

Alternative name(s):
Methionine adenosyltransferase
Short name=MAT

Gene names

Name:	metK
Synonyms:	sams

Organism

Acanthamoeba castellanii (Amoeba)

Taxonomic identifier

5755 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Centramoebida › Acanthamoebidae › Acanthamoeba

Protein attributes

Sequence length	388 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the formation of S-adenosylmethionine from methionine and ATP By similarity.
Catalytic activity	ATP + L-methionine + H2O = phosphate + diphosphate + S-adenosyl-L-methionine.
Cofactor	Binds 2 divalent ions per subunit. Magnesium or cobalt By similarity. Binds 1 potassium ion per subunit By similarity.
Pathway	Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis; S-adenosyl-L-methionine from L-methionine: step 1/1.
Sequence similarities	Belongs to the AdoMet synthase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Ligand	ATP-binding Cobalt Magnesium Metal-binding Nucleotide-binding Potassium
Molecular function	Transferase
Gene Ontology (GO)
Biological process	S-adenosylmethionine biosynthetic process Inferred from electronic annotation. Source: InterPro one-carbon metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW methionine adenosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 388	388	S-adenosylmethionine synthase		PRO_0000174440
Regions
	Nucleotide binding	120 – 125	6	ATP Potential
Sites
	Metal binding	20	1	Magnesium By similarity
	Metal binding	46	1	Potassium By similarity
	Metal binding	272	1	Potassium By similarity
	Metal binding	280	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q95032 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 7CA72AF37B24A408	FASTA	388	42,617
	10 20 30 40 50 60 MASSKTLLFT SECVSESHPD KLCDQVSDAI LDACLANDPY SKVACETASK TGMVMVFGEI 70 80 90 100 110 120 TTKSSFDYQK VIRETVKRIG FTDSSIGFDY KTCNILVAIE QQSPDIAQGV HVGRSDDDLG 130 140 150 160 170 180 AGDQGHMFGH ATNETPEFMP MTHVLATRLC QRMTEVRNNS ILSWMRPDAK TQVTVEYRNE 190 200 210 220 230 240 NGTLIPLRRH TVVISVQHSE DVTNEEIRKR LMEEVIKPSI PAHLLDGETI FHLNPSGRFV 250 260 270 280 290 300 IGGPQGEAGL TGGKIIIDTY GGWGAHGGGA FSGKDPSKVD RSAAYAARWI AKSLVARGLA 310 320 330 340 350 360 NRALVQVSYA IGVSHPLSVF VDSYGTAQGG RTDEDLLAIS KSNFDLRPGK IINDLQLRRP 370 380 IYEKTAYHGH FGRNDPDFLW EAPKKLNF

References

[1]	"Nucleotide sequence and developmental expression of Acanthamoeba S-adenosylmethionine synthetase gene." Ahn K.S., Henney H.R. Jr. Biochim. Biophys. Acta 1351:223-230(1997) [PubMed: 9116037] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X79205 mRNA. Translation: CAA55794.1.
3D structure databases
ProteinModelPortal	Q95032.
SMR	Q95032. Positions 6-388.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR022631. ADOMET_SYNTHASE_CS. IPR022630. S-AdoMet_synt_C. IPR022629. S-AdoMet_synt_central. IPR022628. S-AdoMet_synt_N. IPR002133. S-AdoMet_synthetase. IPR022636. S-AdoMet_synthetase_sfam. [Graphical view]
PANTHER	PTHR11964. S-AdoMet_synt. 1 hit.
Pfam	PF02773. S-AdoMet_synt_C. 1 hit. PF02772. S-AdoMet_synt_M. 1 hit. PF00438. S-AdoMet_synt_N. 1 hit. [Graphical view]
PIRSF	PIRSF000497. MAT. 1 hit.
SUPFAM	SSF55973. S-AdoMet_synt. 3 hits.
TIGRFAMs	TIGR01034. MetK. 1 hit.
PROSITE	PS00376. ADOMET_SYNTHASE_1. 1 hit. PS00377. ADOMET_SYNTHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

METK_ACACA

Accession

Primary (citable) accession number: Q95032

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	February 1, 1997
Last modified:	June 28, 2011
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)