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Protein

L-lactate dehydrogenase

Gene

ImpL3

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

(S)-lactate + NAD+ = pyruvate + NADH.

Pathwayi: pyruvate fermentation to lactate

This protein is involved in step 1 of the subpathway that synthesizes (S)-lactate from pyruvate.
Proteins known to be involved in this subpathway in this organism are:
  1. L-lactate dehydrogenase (ImpL3), L-lactate dehydrogenase (ImpL3), L-lactate dehydrogenase (ImpL3)
This subpathway is part of the pathway pyruvate fermentation to lactate, which is itself part of Fermentation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-lactate from pyruvate, the pathway pyruvate fermentation to lactate and in Fermentation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei99 – 991NADBy similarity
Binding sitei106 – 1061SubstrateBy similarity
Binding sitei138 – 1381NAD or substrateBy similarity
Binding sitei169 – 1691SubstrateBy similarity
Active sitei193 – 1931Proton acceptorBy similarity
Binding sitei248 – 2481SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi29 – 5729NADBy similarityAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • glycolytic process Source: FlyBase
  • myoblast fusion Source: FlyBase
  • somatic muscle development Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Enzyme and pathway databases

ReactomeiR-DME-70268. Pyruvate metabolism.
UniPathwayiUPA00554; UER00611.

Names & Taxonomyi

Protein namesi
Recommended name:
L-lactate dehydrogenase (EC:1.1.1.27)
Short name:
LDH
Gene namesi
Name:ImpL3
Synonyms:Imp-L3
ORF Names:CG10160
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0001258. ImpL3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 332332L-lactate dehydrogenasePRO_0000168494Add
BLAST

Proteomic databases

PaxDbiQ95028.
PRIDEiQ95028.

Expressioni

Gene expression databases

BgeeiFBgn0001258.
ExpressionAtlasiQ95028. differential.
GenevisibleiQ95028. DM.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

BioGridi69949. 54 interactions.
DIPiDIP-17530N.
IntActiQ95028. 2 interactions.
MINTiMINT-1550188.
STRINGi7227.FBpp0304375.

Structurei

3D structure databases

ProteinModelPortaliQ95028.
SMRiQ95028. Positions 3-332.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. LDH family.Curated

Phylogenomic databases

eggNOGiKOG1495. Eukaryota.
COG0039. LUCA.
GeneTreeiENSGT00550000074541.
InParanoidiQ95028.
KOiK00016.
OMAiCNDKLQG.
OrthoDBiEOG091G0HME.
PhylomeDBiQ95028.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00488. Lactate_dehydrog. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR011304. L-lactate_DH.
IPR018177. L-lactate_DH_AS.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01771. L-LDH-NAD. 1 hit.
PROSITEiPS00064. L_LDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q95028-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAIKDSLLA QVAEVLPSSG HKVTIVGIGQ VGMASAFSIL AQNVSKEVCL
60 70 80 90 100
IDVCADKLQG ELMDLQHGSN FLKNPQITAS TDFAASANSR LCIVTAGVRQ
110 120 130 140 150
KEGESRLSLV QRNTDILKNI IPKLVEYSPD TILLMVSNPV DIMTYVAWKL
160 170 180 190 200
SGLPKNRVIG SGTNLDSSRF RFLMSQRLGV APTSCHGWII GEHGDSSVPV
210 220 230 240 250
WSGVNIAGVR LRELNPILGT GEDPEKWNEL HKQVVDSAYE VIKLKGYTSW
260 270 280 290 300
AIGLSTASLA SAILRNTSSV AAVSTSVLGE HGIDKDVFLS LPCVLNANGV
310 320 330
TSVVKQILTP TEVEQLQKSA NIMSDVQAGL KF
Length:332
Mass (Da):35,537
Last modified:February 1, 1997 - v1
Checksum:iE49F1EE2399440F1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U68038 mRNA. Translation: AAB07594.1.
AE014296 Genomic DNA. Translation: AAF50666.1.
RefSeqiNP_001261474.1. NM_001274545.2.
NP_001261475.1. NM_001274546.1.
NP_001261476.1. NM_001274547.1.
NP_476581.1. NM_057233.5.
UniGeneiDm.1468.

Genome annotation databases

EnsemblMetazoaiFBtr0077008; FBpp0076716; FBgn0001258.
FBtr0332065; FBpp0304375; FBgn0001258.
FBtr0332066; FBpp0304376; FBgn0001258.
FBtr0332067; FBpp0304377; FBgn0001258.
GeneIDi45880.
KEGGidme:Dmel_CG10160.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U68038 mRNA. Translation: AAB07594.1.
AE014296 Genomic DNA. Translation: AAF50666.1.
RefSeqiNP_001261474.1. NM_001274545.2.
NP_001261475.1. NM_001274546.1.
NP_001261476.1. NM_001274547.1.
NP_476581.1. NM_057233.5.
UniGeneiDm.1468.

3D structure databases

ProteinModelPortaliQ95028.
SMRiQ95028. Positions 3-332.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi69949. 54 interactions.
DIPiDIP-17530N.
IntActiQ95028. 2 interactions.
MINTiMINT-1550188.
STRINGi7227.FBpp0304375.

Proteomic databases

PaxDbiQ95028.
PRIDEiQ95028.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0077008; FBpp0076716; FBgn0001258.
FBtr0332065; FBpp0304375; FBgn0001258.
FBtr0332066; FBpp0304376; FBgn0001258.
FBtr0332067; FBpp0304377; FBgn0001258.
GeneIDi45880.
KEGGidme:Dmel_CG10160.

Organism-specific databases

CTDi45880.
FlyBaseiFBgn0001258. ImpL3.

Phylogenomic databases

eggNOGiKOG1495. Eukaryota.
COG0039. LUCA.
GeneTreeiENSGT00550000074541.
InParanoidiQ95028.
KOiK00016.
OMAiCNDKLQG.
OrthoDBiEOG091G0HME.
PhylomeDBiQ95028.

Enzyme and pathway databases

UniPathwayiUPA00554; UER00611.
ReactomeiR-DME-70268. Pyruvate metabolism.

Miscellaneous databases

ChiTaRSiImpL3. fly.
GenomeRNAii45880.
PROiQ95028.

Gene expression databases

BgeeiFBgn0001258.
ExpressionAtlasiQ95028. differential.
GenevisibleiQ95028. DM.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00488. Lactate_dehydrog. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR011304. L-lactate_DH.
IPR018177. L-lactate_DH_AS.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01771. L-LDH-NAD. 1 hit.
PROSITEiPS00064. L_LDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLDH_DROME
AccessioniPrimary (citable) accession number: Q95028
Secondary accession number(s): Q9VRW6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: September 7, 2016
This is version 138 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.