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Protein

L-lactate dehydrogenase

Gene

ImpL3

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalytic activityi

(S)-lactate + NAD+ = pyruvate + NADH.

Pathwayi: pyruvate fermentation to lactate

This protein is involved in step 1 of the subpathway that synthesizes (S)-lactate from pyruvate.
Proteins known to be involved in this subpathway in this organism are:
  1. L-lactate dehydrogenase (ImpL3), L-lactate dehydrogenase (Ldh), L-lactate dehydrogenase (ImpL3)
This subpathway is part of the pathway pyruvate fermentation to lactate, which is itself part of Fermentation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-lactate from pyruvate, the pathway pyruvate fermentation to lactate and in Fermentation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei99NADBy similarity1
Binding sitei106SubstrateBy similarity1
Binding sitei138NAD or substrateBy similarity1
Binding sitei169SubstrateBy similarity1
Active sitei193Proton acceptorBy similarity1
Binding sitei248SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi29 – 57NADBy similarityAdd BLAST29

GO - Molecular functioni

GO - Biological processi

  • carbohydrate metabolic process Source: InterPro
  • carboxylic acid metabolic process Source: InterPro
  • myoblast fusion Source: FlyBase
  • somatic muscle development Source: FlyBase

Keywordsi

Molecular functionOxidoreductase
LigandNAD

Enzyme and pathway databases

ReactomeiR-DME-70268 Pyruvate metabolism
UniPathwayiUPA00554; UER00611

Names & Taxonomyi

Protein namesi
Recommended name:
L-lactate dehydrogenase (EC:1.1.1.27)
Short name:
LDH
Gene namesi
Name:ImpL3
Synonyms:Imp-L3
ORF Names:CG10160
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0001258 ImpL3

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001684941 – 332L-lactate dehydrogenaseAdd BLAST332

Proteomic databases

PaxDbiQ95028
PRIDEiQ95028

Expressioni

Gene expression databases

BgeeiFBgn0001258
ExpressionAtlasiQ95028 baseline and differential
GenevisibleiQ95028 DM

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

BioGridi69949, 58 interactors
DIPiDIP-17530N
IntActiQ95028, 3 interactors
STRINGi7227.FBpp0304375

Structurei

3D structure databases

ProteinModelPortaliQ95028
SMRiQ95028
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. LDH family.Curated

Phylogenomic databases

eggNOGiKOG1495 Eukaryota
COG0039 LUCA
GeneTreeiENSGT00550000074541
InParanoidiQ95028
KOiK00016
OMAiMDLMQTA
OrthoDBiEOG091G0HME
PhylomeDBiQ95028

Family and domain databases

Gene3Di3.90.110.10, 1 hit
HAMAPiMF_00488 Lactate_dehydrog, 1 hit
InterProiView protein in InterPro
IPR001557 L-lactate/malate_DH
IPR011304 L-lactate_DH
IPR018177 L-lactate_DH_AS
IPR022383 Lactate/malate_DH_C
IPR001236 Lactate/malate_DH_N
IPR015955 Lactate_DH/Glyco_Ohase_4_C
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF02866 Ldh_1_C, 1 hit
PF00056 Ldh_1_N, 1 hit
PIRSFiPIRSF000102 Lac_mal_DH, 1 hit
PRINTSiPR00086 LLDHDRGNASE
SUPFAMiSSF51735 SSF51735, 1 hit
SSF56327 SSF56327, 1 hit
TIGRFAMsiTIGR01771 L-LDH-NAD, 1 hit
PROSITEiView protein in PROSITE
PS00064 L_LDH, 1 hit

Sequencei

Sequence statusi: Complete.

Q95028-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAIKDSLLA QVAEVLPSSG HKVTIVGIGQ VGMASAFSIL AQNVSKEVCL
60 70 80 90 100
IDVCADKLQG ELMDLQHGSN FLKNPQITAS TDFAASANSR LCIVTAGVRQ
110 120 130 140 150
KEGESRLSLV QRNTDILKNI IPKLVEYSPD TILLMVSNPV DIMTYVAWKL
160 170 180 190 200
SGLPKNRVIG SGTNLDSSRF RFLMSQRLGV APTSCHGWII GEHGDSSVPV
210 220 230 240 250
WSGVNIAGVR LRELNPILGT GEDPEKWNEL HKQVVDSAYE VIKLKGYTSW
260 270 280 290 300
AIGLSTASLA SAILRNTSSV AAVSTSVLGE HGIDKDVFLS LPCVLNANGV
310 320 330
TSVVKQILTP TEVEQLQKSA NIMSDVQAGL KF
Length:332
Mass (Da):35,537
Last modified:February 1, 1997 - v1
Checksum:iE49F1EE2399440F1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U68038 mRNA Translation: AAB07594.1
AE014296 Genomic DNA Translation: AAF50666.1
RefSeqiNP_001261474.1, NM_001274545.2
NP_001261475.1, NM_001274546.1
NP_001261476.1, NM_001274547.1
NP_476581.1, NM_057233.5
UniGeneiDm.1468

Genome annotation databases

EnsemblMetazoaiFBtr0077008; FBpp0076716; FBgn0001258
FBtr0332065; FBpp0304375; FBgn0001258
FBtr0332066; FBpp0304376; FBgn0001258
FBtr0332067; FBpp0304377; FBgn0001258
GeneIDi45880
KEGGidme:Dmel_CG10160

Similar proteinsi

Entry informationi

Entry nameiLDH_DROME
AccessioniPrimary (citable) accession number: Q95028
Secondary accession number(s): Q9VRW6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: May 23, 2018
This is version 154 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families
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