Reviewed, UniProtKB/Swiss-Prot Q95017 (UBC9_CAEEL)

Names and origin

Protein names

Recommended name:
    SUMO-conjugating enzyme UBC9
    EC=6.3.2.-
Alternative name(s):
    SUMO-protein ligase
    Ubiquitin-conjugating enzyme E2 9
    Ubiquitin-protein ligase 9
    Ubiquitin carrier protein 9

Gene names

Name:	ubc-9
ORF Names:	F29B9.6

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	166 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Accepts the ubiquitin-like protein smo-1 from the aos-1-uba-2 E1 complex and catalyzes its covalent attachment to other proteins with the help of an E3 ligase such as gei-17. Required to sumoylate the ETS transcription factor lin-1 and the Polycomb protein sop-2. Required for embryonic development, fertility, vulval morphogenesis and inhibition of vulval cell fates. Ref.3 Ref.4 Ref.5 Ref.6 Ref.7
Catalytic activity	ATP + SUMO + protein lysine = AMP + diphosphate + protein N-SUMOyllysine.
Pathway	Protein modification; protein sumoylation.
Subunit structure	Interacts with smt-3 and sop-2. Ref.1
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Ligand	ATP-binding Nucleotide-binding
Molecular function	Developmental protein Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	embryonic development ending in birth or egg hatching Ref.4 Inferred from mutant phenotype. Source: WormBase growth Inferred from mutant phenotype. Source: WormBase hermaphrodite genitalia development Inferred from mutant phenotype. Source: WormBase locomotion Inferred from mutant phenotype. Source: WormBase modification-dependent protein catabolic process Inferred from electronic annotation. Source: UniProtKB-KW morphogenesis of an epithelium Inferred from mutant phenotype. Source: WormBase negative regulation of vulval development Ref.5 Ref.6 Inferred from mutant phenotype. Source: WormBase nematode larval development Inferred from mutant phenotype. Source: WormBase positive regulation of growth rate Inferred from mutant phenotype. Source: WormBase post-translational protein modification Inferred from electronic annotation. Source: InterPro regulation of protein metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct small conjugating protein ligase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
atn-1	Q23158	1	EBI-328938,EBI-314014

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 166	166	SUMO-conjugating enzyme UBC9		PRO_0000082517
Sites
	Active site	93	1	Glycyl thioester intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q95017-1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: E5A5AFA773DE286C	FASTA	166	19,115
	10 20 30 40 50 60 MSGIAAGRLA EERKHWRKDH PFGFIAKPVK NADGTLNLFN WECAIPGRKD TIWEGGLYRI 70 80 90 100 110 120 RMLFKDDFPS TPPKCKFEPP LFHPNVYPSG TVCLSLLDEN KDWKPSISIK QLLIGIQDLL 130 140 150 160 NHPNIEDPAQ AEAYQIYCQN RAEYEKRVKK EAVKYAAELV QKQMLE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Ubc-9 of Caenorhabditis elegans: identification, characterization and interaction with smt-3." Li T., Sun B., Lee M.-K., Teo T.-S. Submitted (NOV-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH SMT-3. Strain: Bristol N2.
[2]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[3]	"Functional and phylogenetic analysis of the ubiquitylation system in Caenorhabditis elegans: ubiquitin-conjugating enzymes, ubiquitin-activating enzymes, and ubiquitin-like proteins." Jones D., Crowe E., Stevens T.A., Candido E.P.M. Genome Biol. 3:RESEARCH0002.1-RESEARCH0002.15(2002) [PubMed: 11806825] [Abstract] Cited for: FUNCTION.
[4]	"SUMO modification is required for in vivo Hox gene regulation by the Caenorhabditis elegans Polycomb group protein SOP-2." Zhang H., Smolen G.A., Palmer R., Christoforou A., van den Heuvel S., Haber D.A. Nat. Genet. 36:507-511(2004) [PubMed: 15107848] [Abstract] Cited for: FUNCTION.
[5]	"Sumoylation of LIN-1 promotes transcriptional repression and inhibition of vulval cell fates." Leight E.R., Glossip D., Kornfeld K. Development 132:1047-1056(2005) [PubMed: 15689373] [Abstract] Cited for: FUNCTION.
[6]	"Chromatin regulation and sumoylation in the inhibition of Ras-induced vulval development in Caenorhabditis elegans." Poulin G., Dong Y., Fraser A.G., Hopper N.A., Ahringer J. EMBO J. 24:2613-2623(2005) [PubMed: 15990876] [Abstract] Cited for: FUNCTION.
[7]	"The T-box factor TBX-2 and the SUMO conjugating enzyme UBC-9 are required for ABa-derived pharyngeal muscle in C. elegans." Roy Chowdhuri S., Crum T., Woollard A., Aslam S., Okkema P.G. Dev. Biol. 295:664-677(2006) [PubMed: 16701625] [Abstract] Cited for: FUNCTION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF106565 mRNA. Translation: AAC97374.1. U70849 Genomic DNA. Translation: AAK67232.1.
PIR	T29929.
RefSeq	NP_001023158.1.
UniGene	Cel.6281
3D structure databases
HSSP	HSSP built from PDB template 1U9B based on UniProtKB P50550.
SMR	Q95017. Positions 1-156.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:25778N.
IntAct	Q95017. 5 interactions.
Genome annotation databases
Ensembl	F29B9.6. Caenorhabditis elegans. [Contig view]
GeneID	3565767.
KEGG	cel:F29B9.6.
Organism-specific databases
WormBase	WBGene00006706. ubc-9.
WormPep	F29B9.6. CE09784. [WorfDB]
Phylogenomic databases
OMA	Q95017. KQWRKDH.
Gene expression databases
ArrayExpress	Q95017.
Family and domain databases
InterPro	IPR016135. UBQ-conjugat/RWD-like. IPR000608. UBQ-conjugat_E2. [Graphical view]
Gene3D	G3DSA:3.10.110.10. UBQ-conjugat_E2. 1 hit.
Pfam	PF00179. UQ_con. 1 hit. [Graphical view]
ProDom	PD000461. UBQ_conjugat. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00212. UBCc. 1 hit. [Graphical view]
PROSITE	PS00183. UBIQUITIN_CONJUGAT_1. 1 hit. PS50127. UBIQUITIN_CONJUGAT_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	957785.

Entry information

Entry name

UBC9_CAEEL

Accession

Primary (citable) accession number: Q95017
Secondary accession number(s): Q9GYI5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2004
Last sequence update:	February 1, 1997
Last modified:	June 16, 2009
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project