Q95017 (UBC9_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
SUMO-conjugating enzyme UBC9
EC=6.3.2.-

Alternative name(s):
SUMO-protein ligase
Ubiquitin carrier protein 9
Ubiquitin-conjugating enzyme E2 9
Ubiquitin-protein ligase 9

Gene names

Name:	ubc-9
ORF Names:	F29B9.6

Organism

Caenorhabditis elegans

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	166 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Accepts the ubiquitin-like protein smo-1 from the aos-1-uba-2 E1 complex and catalyzes its covalent attachment to other proteins with the help of an E3 ligase such as gei-17. Required to sumoylate the ETS transcription factor lin-1 and the Polycomb protein sop-2. Required for embryonic development, fertility, vulval morphogenesis and inhibition of vulval cell fates. Ref.3 Ref.4 Ref.5 Ref.6 Ref.7 Ref.8
Catalytic activity	ATP + SUMO + protein lysine = AMP + diphosphate + protein N-SUMOyllysine.
Pathway	Protein modification; protein sumoylation.
Subunit structure	Interacts with brd-1 and rad-51. Interacts with smo-1 and sop-2. Ref.1 Ref.4
Disruption phenotype	Sterility, embryonic lethality and sensitivity to radiation. Ref.4
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Ligand	ATP-binding Nucleotide-binding
Molecular function	Developmental protein Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	anterior/posterior pattern specification Inferred from mutant phenotype Ref.5. Source: WormBase cellular response to stress Inferred from mutant phenotype Ref.4. Source: WormBase embryo development ending in birth or egg hatching Inferred from mutant phenotype Ref.3Ref.4Ref.5. Source: WormBase growth Inferred from mutant phenotype. Source: WormBase hermaphrodite genitalia development Inferred from mutant phenotype. Source: WormBase locomotion Inferred from mutant phenotype. Source: WormBase morphogenesis of an epithelium Inferred from mutant phenotype. Source: WormBase negative regulation of transcription from RNA polymerase II promoter Inferred from mutant phenotype Ref.5. Source: WormBase negative regulation of vulval development Inferred from mutant phenotype Ref.6Ref.7. Source: WormBase oviposition Inferred from mutant phenotype Ref.3. Source: WormBase pharyngeal muscle development Inferred from mutant phenotype Ref.8. Source: WormBase positive regulation of growth rate Inferred from mutant phenotype. Source: WormBase protein sumoylation Inferred from direct assay Ref.5. Source: WormBase regulation of protein localization Inferred from mutant phenotype Ref.8. Source: WormBase vulval development Inferred from mutant phenotype Ref.3. Source: WormBase
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA polymerase II transcription factor binding Inferred from physical interaction Ref.8. Source: WormBase SAM domain binding Inferred from physical interaction Ref.5. Source: WormBase SUMO ligase activity Inferred from direct assay Ref.5. Source: WormBase
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
brd-1	Q21209	4	EBI-328938,EBI-3895480
rad-51	Q95Q25	4	EBI-328938,EBI-321895
smo-1	P55853	5	EBI-328938,EBI-313647

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 166	166	SUMO-conjugating enzyme UBC9		PRO_0000082517
Sites
	Active site	93	1	Glycyl thioester intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q95017 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: E5A5AFA773DE286C	FASTA	166	19,115
	10 20 30 40 50 60 MSGIAAGRLA EERKHWRKDH PFGFIAKPVK NADGTLNLFN WECAIPGRKD TIWEGGLYRI 70 80 90 100 110 120 RMLFKDDFPS TPPKCKFEPP LFHPNVYPSG TVCLSLLDEN KDWKPSISIK QLLIGIQDLL 130 140 150 160 NHPNIEDPAQ AEAYQIYCQN RAEYEKRVKK EAVKYAAELV QKQMLE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Ubc-9 of Caenorhabditis elegans: identification, characterization and interaction with smt-3." Li T., Sun B., Lee M.-K., Teo T.-S. Submitted (NOV-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH SMO-1. Strain: Bristol N2.
[2]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[3]	"Functional and phylogenetic analysis of the ubiquitylation system in Caenorhabditis elegans: ubiquitin-conjugating enzymes, ubiquitin-activating enzymes, and ubiquitin-like proteins." Jones D., Crowe E., Stevens T.A., Candido E.P.M. Genome Biol. 3:RESEARCH0002.1-RESEARCH0002.15(2002) [PubMed: 11806825] [Abstract] Cited for: FUNCTION.
[4]	"BRCA1/BARD1 orthologs required for DNA repair in Caenorhabditis elegans." Boulton S.J., Martin J.S., Polanowska J., Hill D.E., Gartner A., Vidal M. Curr. Biol. 14:33-39(2004) [PubMed: 14711411] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE, INTERACTION WITH BRD-1 AND RAD-1.
[5]	"SUMO modification is required for in vivo Hox gene regulation by the Caenorhabditis elegans Polycomb group protein SOP-2." Zhang H., Smolen G.A., Palmer R., Christoforou A., van den Heuvel S., Haber D.A. Nat. Genet. 36:507-511(2004) [PubMed: 15107848] [Abstract] Cited for: FUNCTION.
[6]	"Sumoylation of LIN-1 promotes transcriptional repression and inhibition of vulval cell fates." Leight E.R., Glossip D., Kornfeld K. Development 132:1047-1056(2005) [PubMed: 15689373] [Abstract] Cited for: FUNCTION.
[7]	"Chromatin regulation and sumoylation in the inhibition of Ras-induced vulval development in Caenorhabditis elegans." Poulin G., Dong Y., Fraser A.G., Hopper N.A., Ahringer J. EMBO J. 24:2613-2623(2005) [PubMed: 15990876] [Abstract] Cited for: FUNCTION.
[8]	"The T-box factor TBX-2 and the SUMO conjugating enzyme UBC-9 are required for ABa-derived pharyngeal muscle in C. elegans." Roy Chowdhuri S., Crum T., Woollard A., Aslam S., Okkema P.G. Dev. Biol. 295:664-677(2006) [PubMed: 16701625] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF106565 mRNA. Translation: AAC97374.1. FO081255 Genomic DNA. Translation: CCD70229.1.
PIR	T29929.
RefSeq	NP_001023158.1. NM_001027987.2.
UniGene	Cel.6281.
3D structure databases
ProteinModelPortal	Q95017.
SMR	Q95017. Positions 1-156.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-25778N.
IntAct	Q95017. 11 interactions.
MINT	MINT-229351.
STRING	Q95017.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	F29B9.6.1; F29B9.6.1; F29B9.6. F29B9.6.2; F29B9.6.2; F29B9.6.
GeneID	3565767.
KEGG	cel:F29B9.6.
UCSC	F29B9.6.1. c. elegans.
Organism-specific databases
CTD	3565767.
WormBase	F29B9.6; CE09784; WBGene00006706; ubc-9.
Phylogenomic databases
eggNOG	meNOG06611.
GeneTree	EMGT00070000025728.
HOGENOM	HBG756483.
InParanoid	Q95017.
OMA	KQWRKDH.
PhylomeDB	Q95017.
Gene expression databases
ArrayExpress	Q95017.
Family and domain databases
InterPro	IPR000608. UBQ-conjugat_E2. IPR023313. UBQ-conjugating_AS. IPR016135. UBQ-conjugating_enzyme/RWD. [Graphical view]
Gene3D	G3DSA:3.10.110.10. UBQ-conjugat_E2. 1 hit.
KO	K10577.
Pfam	PF00179. UQ_con. 1 hit. [Graphical view]
SUPFAM	SSF54495. UBQ-conjugat/RWD-like. 1 hit.
PROSITE	PS00183. UBIQUITIN_CONJUGAT_1. 1 hit. PS50127. UBIQUITIN_CONJUGAT_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	957785.

Entry information

Entry name

UBC9_CAEEL

Accession

Primary (citable) accession number: Q95017
Secondary accession number(s): Q9GYI5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2004
Last sequence update:	February 1, 1997
Last modified:	January 25, 2012
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project