Q95000 (CHLE1_BRALA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cholinesterase 1
EC=3.1.1.8

Gene names

Name:

CHE1

Organism

Branchiostoma lanceolatum (Common lancelet) (Amphioxus)

Taxonomic identifier

7740 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Cephalochordata › Branchiostomidae › Branchiostoma

Protein attributes

Sequence length	357 AA.
Sequence status	Fragment.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	An acylcholine + H2O = choline + a carboxylate.
Sequence similarities	Belongs to the type-B carboxylesterase/lipase family.

Ontologies

Keywords
Molecular function	Hydrolase Serine esterase
PTM	Disulfide bond
Gene Ontology (GO)
Molecular_function	carboxylesterase activity Inferred from electronic annotation. Source: UniProtKB-KW cholinesterase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – ›357	›357	Cholinesterase 1		PRO_0000070289
Sites
	Active site	112	1	Acyl-ester intermediate By similarity
	Active site	244	1	Charge relay system By similarity
	Active site	357	1	Charge relay system By similarity
Amino acid modifications
	Disulfide bond	166 ↔ 179		By similarity
Experimental info
	Non-terminal residue	1	1
	Non-terminal residue	357	1

Sequences

	Sequence		Length	Mass (Da)
	Q95000 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: A2CBDE841704DCDF	FASTA	357	39,462
	10 20 30 40 50 60 TPISEDCLYL NVWQPSPAPT GATVLVWIYG GGFFSGTSSL DVYDGRYLAR MEDVVVVSMN 70 80 90 100 110 120 YRLGALGFLY TGSEAAPGNA GLLDQHLALQ WVQQNIQSFG GDPGKVTIFG ESAGAASVNF 130 140 150 160 170 180 HMLSPMSRDL FQRAMMHSAS ALAPWAVTPS EQARQRSKAL AIDIGCSADE EDMDVLVACL 190 200 210 220 230 240 REVSAQTILD HEWNVVDLSD AHFLADIPFP PVKDGRFITE DPAEMYAAGN FKDIDILVGF 250 260 270 280 290 300 VKDEGNFWLV YGVPGFDKDT DSIIDRETFV GDIVFCHPRL NDITVERTAF EYTDWLHMDQ 310 320 330 340 350 DTMYRDALDS VFGDPFFVCP TMAVGKAHVN HGRTAYVYEF AQVASNLAWP HWMGAMH

References

[1]

"Two cholinesterase activities and genes are present in amphioxus." Sutherland D., McClellan J.S., Milner D., Soong W., Axon N., Sanders M., Hester A., Kao Y.H., Poczatek T., Routt S., Pezzementi L. J. Exp. Zool. 277:213-229(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	U74378 Genomic DNA. Translation: AAB18262.1.
3D structure databases
ProteinModelPortal	Q95000.
SMR	Q95000. Positions 2-357.
ModBase	Search...
Protein family/group databases
MEROPS	S09.980.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG008839.
Family and domain databases
InterPro	IPR002018. CarbesteraseB. IPR019826. Carboxylesterase_B_AS. IPR019819. Carboxylesterase_B_CS. IPR000997. Cholinesterase. [Graphical view]
Pfam	PF00135. COesterase. 1 hit. [Graphical view]
PRINTS	PR00878. CHOLNESTRASE.
PROSITE	PS00122. CARBOXYLESTERASE_B_1. 1 hit. PS00941. CARBOXYLESTERASE_B_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHLE1_BRALA

Accession

Primary (citable) accession number: Q95000

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	April 3, 2013
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program