Q94L32 (NMT2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative glycylpeptide N-tetradecanoyltransferase 2
EC=2.3.1.97

Alternative name(s):
Myristoyl-CoA:protein N-myristoyltransferase 2
Short name=NMT 2
Short name=Type I N-myristoyltransferase 2
Peptide N-myristoyltransferase 2

Gene names

Name:	NMT2
Ordered Locus Names:	At2g44170
ORF Names:	F6E13.30

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	430 AA.
Sequence status	Complete.
Protein existence	Uncertain

General annotation (Comments)

Function	May add a myristoyl group to the N-terminal glycine residue of certain cellular proteins By similarity.
Catalytic activity	Tetradecanoyl-CoA + glycylpeptide = CoA + N-tetradecanoylglycylpeptide.
Sequence similarities	Belongs to the NMT family.
Caution	Could be the product of a pseudogene.
Sequence caution	The sequence AAC23421.2 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	N-terminal protein myristoylation Inferred from electronic annotation. Source: InterPro
Molecular function	glycylpeptide N-tetradecanoyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 430	430	Putative glycylpeptide N-tetradecanoyltransferase 2		PRO_0000064232
Regions
	Region	47 – 50	4	Myristoyl CoA-binding By similarity
	Region	178 – 214	37	Myristoyl CoA-binding By similarity
Sites
	Active site	430	1	Proton acceptor; via carboxylate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q94L32 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 08F2333ED0E23035	FASTA	430	49,628
	10 20 30 40 50 60 MSDPKLKPVE DALVTVAKSS QIQLAKDDTS GGTIVGRLLQ CQYSKTHKFW ETQPVEQFKD 70 80 90 100 110 120 IQDTSLPEGP IEPATLVSEV KQEPYNLLGQ FEWTICDMNS DDMCLEMYNF LKENSPDDQQ 130 140 150 160 170 180 IKYEYSKEYL RWALCPPGYY QSWHIGVRVK TSKKLIAFIC GRPTRIRVRD EVVKMAKVNS 190 200 210 220 230 240 LCVHKKLRSK GLAPLMIKEL TRRVKLQNIW QAAYTSSHIL SRPVTTSRDW VRMLNPKKLI 250 260 270 280 290 300 DVGLTRLRDR MTMSRTVKLY KLPDAPITPG FREMERRDVP AVTALLRNYL SQFAVATDFD 310 320 330 340 350 360 DNDVKHWLLP RENIVYSYVV VSPETHDVTD FCSFCNSSIT IPGNRKYTTL ECAYACCNVA 370 380 390 400 410 420 TLTSLSQLVN DALIVSKQKG FDVFYASDVM QNESFLKELR FYPLCRQSHY YLYNYRLRNA 430 LKPSELGLIL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Unexpected protein families including cell defense components feature in the N-myristoylome of a higher eukaryote." Boisson B., Giglione C., Meinnel T. J. Biol. Chem. 278:43418-43429(2003) [PubMed: 12912986] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF250957 mRNA. Translation: AAK49038.1. AC004005 Genomic DNA. Translation: AAC23421.2. Sequence problems.
IPI	IPI00519954.
PIR	T00697.
3D structure databases
ProteinModelPortal	Q94L32.
SMR	Q94L32. Positions 17-430.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
TAIR	At2g44170.
Phylogenomic databases
eggNOG	KOG2779.
PhylomeDB	Q94L32.
Gene expression databases
Genevestigator	Q94L32.
Family and domain databases
InterPro	IPR016181. Acyl_CoA_acyltransferase. IPR000903. MyristoylCoA_TrFase. IPR022677. MyristoylCoA_TrFase_C. IPR022678. MyristoylCoA_TrFase_CS. IPR022676. MyristoylCoA_TrFase_N. [Graphical view]
Gene3D	G3DSA:3.40.630.30. Acyl_CoA_acyltransferase. 2 hits.
PANTHER	PTHR11377. Myristoyl_trans. 1 hit.
Pfam	PF01233. NMT. 1 hit. PF02799. NMT_C. 1 hit. [Graphical view]
PIRSF	PIRSF015892. N-myristl_transf. 1 hit.
SUPFAM	SSF55729. Acyl_CoA_acyltransferase. 2 hits.
PROSITE	PS00975. NMT_1. 1 hit. PS00976. NMT_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NMT2_ARATH

Accession

Primary (citable) accession number: Q94L32
Secondary accession number(s): O80586

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2006
Last sequence update:	December 1, 2001
Last modified:	September 21, 2011
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program