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Protein

Two pore calcium channel protein 1

Gene

TPC1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions as a voltage-gated inward-rectifying Ca2+ channel (VDCC) across the vacuole membrane. Is one of the essential components of the slow vacuolar (SV) channel. Acts as the major ROS-responsive Ca2+ channel and is the possible target of Al-dependent inhibition. Involved in the regulation of germination and stomatal movement.2 Publications

Enzyme regulationi

Inhibited by Al3+.

GO - Molecular functioni

  • calcium ion binding Source: InterPro
  • voltage-gated calcium channel activity Source: TAIR

GO - Biological processi

  • calcium ion transport Source: TAIR
  • calcium-mediated signaling Source: TAIR
  • membrane depolarization during action potential Source: GO_Central
  • regulation of jasmonic acid biosynthetic process Source: TAIR
  • regulation of stomatal movement Source: TAIR
  • seed germination Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Calcium channel, Ion channel, Voltage-gated channel

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

Calcium

Enzyme and pathway databases

ReactomeiR-ATH-2672351. Stimuli-sensing channels.

Protein family/group databases

TCDBi1.A.1.11.26. the voltage-gated ion channel (vic) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Two pore calcium channel protein 1
Alternative name(s):
Calcium channel protein 1
Short name:
AtCCH1
Fatty acid oxygenation up-regulated protein 2
Voltage-dependent calcium channel protein TPC1
Short name:
AtTPC1
Gene namesi
Name:TPC1
Synonyms:CCH1, FOU2
Ordered Locus Names:At4g03560
ORF Names:F9H3.19, T5L23.5
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G03560.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 71CytoplasmicSequence analysisAdd BLAST71
Transmembranei72 – 92Helical; Name=S1 of repeat ISequence analysisAdd BLAST21
Topological domaini93 – 120VacuolarSequence analysisAdd BLAST28
Transmembranei121 – 141Helical; Name=S2 of repeat ISequence analysisAdd BLAST21
Topological domaini142 – 158CytoplasmicSequence analysisAdd BLAST17
Transmembranei159 – 179Helical; Name=S3 of repeat ISequence analysisAdd BLAST21
Topological domaini180VacuolarSequence analysis1
Transmembranei181 – 199Helical; Voltage-sensor; Name=S4 of repeat ISequence analysisAdd BLAST19
Topological domaini200 – 218CytoplasmicSequence analysisAdd BLAST19
Transmembranei219 – 239Helical; Name=S5 of repeat ISequence analysisAdd BLAST21
Topological domaini240 – 245VacuolarSequence analysis6
Intramembranei246 – 260Pore-forming; Name=Pore-forming 1Add BLAST15
Topological domaini261 – 282VacuolarSequence analysisAdd BLAST22
Transmembranei283 – 303Helical; Name=S6 of repeat ISequence analysisAdd BLAST21
Topological domaini304 – 428CytoplasmicSequence analysisAdd BLAST125
Transmembranei429 – 449Helical; Name=S1 of repeat IISequence analysisAdd BLAST21
Topological domaini450 – 465VacuolarSequence analysisAdd BLAST16
Transmembranei466 – 486Helical; Name=S2 of repeat IISequence analysisAdd BLAST21
Topological domaini487 – 498CytoplasmicSequence analysisAdd BLAST12
Transmembranei499 – 519Helical; Name=S3 of repeat IISequence analysisAdd BLAST21
Topological domaini520 – 528VacuolarSequence analysis9
Transmembranei529 – 546Helical; Voltage-sensor; Name=S4 of repeat IISequence analysisAdd BLAST18
Topological domaini547 – 557CytoplasmicSequence analysisAdd BLAST11
Transmembranei558 – 578Helical; Name=S5 of repeat IISequence analysisAdd BLAST21
Topological domaini579 – 615VacuolarSequence analysisAdd BLAST37
Intramembranei616 – 630Pore-forming; Name=Pore-forming 2Add BLAST15
Topological domaini631 – 651VacuolarSequence analysisAdd BLAST21
Transmembranei652 – 672Helical; Name=S6 of repeat IISequence analysisAdd BLAST21
Topological domaini673 – 733CytoplasmicSequence analysisAdd BLAST61

GO - Cellular componenti

  • Golgi apparatus Source: TAIR
  • integral component of membrane Source: UniProtKB-KW
  • plant-type vacuole Source: TAIR
  • plasma membrane Source: TAIR
  • vacuolar membrane Source: TAIR
  • vacuole Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Vacuole

Pathology & Biotechi

Disruption phenotypei

Plants display an impairment of both the Ca2+ inhibition of stomatal guard cell opening and abscisic acid (ABA) inhibition of seed germination.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi454D → N in fou2; shows elevated LOX and AOS activity levels and an increased resistance to B.cinerea. Strongly increases oxylipin biogenesis in response to wounding. 2 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003431691 – 733Two pore calcium channel protein 1Add BLAST733

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ94KI8.
PRIDEiQ94KI8.

PTM databases

SwissPalmiQ94KI8.

Expressioni

Tissue specificityi

Ubiquitously expressed.1 Publication

Gene expression databases

ExpressionAtlasiQ94KI8. baseline and differential.
GenevisibleiQ94KI8. AT.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi10969. 8 interactors.
DIPiDIP-39873N.
IntActiQ94KI8. 1 interactor.
STRINGi3702.AT4G03560.1.

Structurei

Secondary structure

1733
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi33 – 46Combined sources14
Helixi62 – 71Combined sources10
Helixi73 – 91Combined sources19
Beta strandi95 – 97Combined sources3
Beta strandi98 – 102Combined sources5
Beta strandi106 – 108Combined sources3
Helixi117 – 136Combined sources20
Helixi137 – 141Combined sources5
Helixi144 – 149Combined sources6
Helixi151 – 172Combined sources22
Helixi189 – 196Combined sources8
Helixi200 – 237Combined sources38
Beta strandi239 – 241Combined sources3
Helixi242 – 244Combined sources3
Turni245 – 250Combined sources6
Helixi251 – 262Combined sources12
Turni263 – 266Combined sources4
Helixi267 – 270Combined sources4
Helixi272 – 277Combined sources6
Helixi281 – 294Combined sources14
Helixi296 – 334Combined sources39
Beta strandi339 – 343Combined sources5
Helixi344 – 357Combined sources14
Helixi367 – 376Combined sources10
Beta strandi382 – 384Combined sources3
Helixi385 – 397Combined sources13
Helixi418 – 427Combined sources10
Helixi430 – 456Combined sources27
Helixi461 – 486Combined sources26
Helixi488 – 493Combined sources6
Helixi495 – 516Combined sources22
Helixi525 – 532Combined sources8
Helixi533 – 547Combined sources15
Turni549 – 551Combined sources3
Helixi552 – 586Combined sources35
Helixi595 – 602Combined sources8
Helixi605 – 609Combined sources5
Beta strandi613 – 615Combined sources3
Helixi616 – 628Combined sources13
Helixi633 – 643Combined sources11
Helixi648 – 659Combined sources12
Turni660 – 662Combined sources3
Helixi663 – 685Combined sources23

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5DQQX-ray2.87A12-733[»]
5E1JX-ray3.31A1-733[»]
ProteinModelPortaliQ94KI8.
SMRiQ94KI8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini322 – 357EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini363 – 398EF-hand 2PROSITE-ProRule annotationAdd BLAST36

Domaini

Each of the two internal repeats contains five hydrophobic transmembrane segments (S1, S2, S3, S5, S6) and one positively charged transmembrane segment (S4). S4 segments probably represent the voltage-sensor and are characterized by a series of positively charged amino acids (By similarity).By similarity

Sequence similaritiesi

Contains 2 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410INF7. Eukaryota.
ENOG410XZT8. LUCA.
HOGENOMiHOG000029959.
InParanoidiQ94KI8.
KOiK16900.
OMAiWIYVIEM.
OrthoDBiEOG093603ZA.
PhylomeDBiQ94KI8.

Family and domain databases

CDDicd00051. EFh. 1 hit.
Gene3Di1.10.238.10. 1 hit.
1.20.120.350. 1 hit.
InterProiIPR027359. Channel_four-helix_dom.
IPR011992. EF-hand-dom_pair.
IPR002048. EF_hand_dom.
IPR005821. Ion_trans_dom.
[Graphical view]
PfamiPF00520. Ion_trans. 2 hits.
[Graphical view]
SMARTiSM00054. EFh. 2 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS50222. EF_HAND_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q94KI8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEDPLIGRDS LGGGGTDRVR RSEAITHGTP FQKAAALVDL AEDGIGLPVE
60 70 80 90 100
ILDQSSFGES ARYYFIFTRL DLIWSLNYFA LLFLNFFEQP LWCEKNPKPS
110 120 130 140 150
CKDRDYYYLG ELPYLTNAES IIYEVITLAI LLVHTFFPIS YEGSRIFWTS
160 170 180 190 200
RLNLVKVACV VILFVDVLVD FLYLSPLAFD FLPFRIAPYV RVIIFILSIR
210 220 230 240 250
ELRDTLVLLS GMLGTYLNIL ALWMLFLLFA SWIAFVMFED TQQGLTVFTS
260 270 280 290 300
YGATLYQMFI LFTTSNNPDV WIPAYKSSRW SSVFFVLYVL IGVYFVTNLI
310 320 330 340 350
LAVVYDSFKE QLAKQVSGMD QMKRRMLEKA FGLIDSDKNG EIDKNQCIKL
360 370 380 390 400
FEQLTNYRTL PKISKEEFGL IFDELDDTRD FKINKDEFAD LCQAIALRFQ
410 420 430 440 450
KEEVPSLFEH FPQIYHSALS QQLRAFVRSP NFGYAISFIL IINFIAVVVE
460 470 480 490 500
TTLDIEESSA QKPWQVAEFV FGWIYVLEMA LKIYTYGFEN YWREGANRFD
510 520 530 540 550
FLVTWVIVIG ETATFITPDE NTFFSNGEWI RYLLLARMLR LIRLLMNVQR
560 570 580 590 600
YRAFIATFIT LIPSLMPYLG TIFCVLCIYC SIGVQVFGGL VNAGNKKLFE
610 620 630 640 650
TELAEDDYLL FNFNDYPNGM VTLFNLLVMG NWQVWMESYK DLTGTWWSIT
660 670 680 690 700
YFVSFYVITI LLLLNLVVAF VLEAFFTELD LEEEEKCQGQ DSQEKRNRRR
710 720 730
SAGSKSRSQR VDTLLHHMLG DELSKPECST SDT
Length:733
Mass (Da):84,873
Last modified:December 1, 2001 - v1
Checksum:i1CA978D6B8BFF445
GO

Sequence cautioni

The sequence AAD11598 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAD15312 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAB77841 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti33K → R in BAB55460 (PubMed:11577183).Curated1
Sequence conflicti256Y → F in BAB55460 (PubMed:11577183).Curated1
Sequence conflicti612N → T in BAB55460 (PubMed:11577183).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB053952 mRNA. Translation: BAB55460.1.
AF360372 mRNA. Translation: AAK39554.1.
AC005142 Genomic DNA. Translation: AAD15312.1. Sequence problems.
AF071527 Genomic DNA. Translation: AAD11598.1. Sequence problems.
AL161497 Genomic DNA. Translation: CAB77841.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE82337.1.
PIRiB85045.
RefSeqiNP_567258.1. NM_116594.5.
UniGeneiAt.3957.

Genome annotation databases

EnsemblPlantsiAT4G03560.1; AT4G03560.1; AT4G03560.
GeneIDi825655.
GrameneiAT4G03560.1; AT4G03560.1; AT4G03560.
KEGGiath:AT4G03560.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB053952 mRNA. Translation: BAB55460.1.
AF360372 mRNA. Translation: AAK39554.1.
AC005142 Genomic DNA. Translation: AAD15312.1. Sequence problems.
AF071527 Genomic DNA. Translation: AAD11598.1. Sequence problems.
AL161497 Genomic DNA. Translation: CAB77841.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE82337.1.
PIRiB85045.
RefSeqiNP_567258.1. NM_116594.5.
UniGeneiAt.3957.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5DQQX-ray2.87A12-733[»]
5E1JX-ray3.31A1-733[»]
ProteinModelPortaliQ94KI8.
SMRiQ94KI8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi10969. 8 interactors.
DIPiDIP-39873N.
IntActiQ94KI8. 1 interactor.
STRINGi3702.AT4G03560.1.

Protein family/group databases

TCDBi1.A.1.11.26. the voltage-gated ion channel (vic) superfamily.

PTM databases

SwissPalmiQ94KI8.

Proteomic databases

PaxDbiQ94KI8.
PRIDEiQ94KI8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G03560.1; AT4G03560.1; AT4G03560.
GeneIDi825655.
GrameneiAT4G03560.1; AT4G03560.1; AT4G03560.
KEGGiath:AT4G03560.

Organism-specific databases

TAIRiAT4G03560.

Phylogenomic databases

eggNOGiENOG410INF7. Eukaryota.
ENOG410XZT8. LUCA.
HOGENOMiHOG000029959.
InParanoidiQ94KI8.
KOiK16900.
OMAiWIYVIEM.
OrthoDBiEOG093603ZA.
PhylomeDBiQ94KI8.

Enzyme and pathway databases

ReactomeiR-ATH-2672351. Stimuli-sensing channels.

Miscellaneous databases

PROiQ94KI8.

Gene expression databases

ExpressionAtlasiQ94KI8. baseline and differential.
GenevisibleiQ94KI8. AT.

Family and domain databases

CDDicd00051. EFh. 1 hit.
Gene3Di1.10.238.10. 1 hit.
1.20.120.350. 1 hit.
InterProiIPR027359. Channel_four-helix_dom.
IPR011992. EF-hand-dom_pair.
IPR002048. EF_hand_dom.
IPR005821. Ion_trans_dom.
[Graphical view]
PfamiPF00520. Ion_trans. 2 hits.
[Graphical view]
SMARTiSM00054. EFh. 2 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS50222. EF_HAND_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTPC1_ARATH
AccessioniPrimary (citable) accession number: Q94KI8
Secondary accession number(s): Q948T1, Q9ZT83
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 2008
Last sequence update: December 1, 2001
Last modified: November 30, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.