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Protein

Lariat debranching enzyme

Gene

DBR1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Cleaves the 2'-5' phosphodiester linkage at the branch point of lariat intron pre-mRNAs after splicing and converts them into linear molecules that are subsequently degraded. It thereby facilitates ribonucleotide turnover. It may also participate in retrovirus replication via an RNA lariat intermediate in cDNA synthesis. Plays en essential role during embryogenesis.1 Publication

Catalytic activityi

RNA processing activity that hydrolyzes the 2'-5' phosphodiester linkage at the branchpoint of excised intron lariats.

GO - Molecular functioni

  • RNA lariat debranching enzyme activity Source: TAIR

GO - Biological processi

  • embryo development ending in seed dormancy Source: TAIR
  • mRNA splicing, via spliceosome Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Hydrolase

Keywords - Biological processi

mRNA processing

Names & Taxonomyi

Protein namesi
Recommended name:
Lariat debranching enzyme (EC:3.1.-.-)
Short name:
AtDBR1
Gene namesi
Name:DBR1
Ordered Locus Names:At4g31770
ORF Names:F28M20.40
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G31770.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 418418Lariat debranching enzymePRO_0000250369Add
BLAST

Proteomic databases

PaxDbiQ94K01.
PRIDEiQ94K01.

Expressioni

Tissue specificityi

Widely expressed. Expressed in roots, stems, cauline and rosette leaves, flower buds and siliques.1 Publication

Gene expression databases

ExpressionAtlasiQ94K01. baseline and differential.
GenevisibleiQ94K01. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT4G31770.1.

Structurei

3D structure databases

ProteinModelPortaliQ94K01.
SMRiQ94K01. Positions 3-251.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the lariat debranching enzyme family.Curated

Phylogenomic databases

eggNOGiKOG2863. Eukaryota.
ENOG410XP0F. LUCA.
HOGENOMiHOG000216468.
InParanoidiQ94K01.
KOiK18328.
OMAiEYGNWQK.
PhylomeDBiQ94K01.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR007708. DBR1_C.
IPR029052. Metallo-depent_PP-like.
[Graphical view]
PfamiPF05011. DBR1. 1 hit.
PF00149. Metallophos. 1 hit.
[Graphical view]
SMARTiSM01124. DBR1. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.

Sequencei

Sequence statusi: Complete.

Q94K01-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIAIEGCMH GDLDNVYKTI QHYEQIHNTK VDLLLCCGDF QAVRNEKDMD
60 70 80 90 100
SLNVPRKYRE MKSFWKYYSG QEVAPIPTIF IGGNHEASNY LWELYYGGWA
110 120 130 140 150
ATNIYFLGFA GVVKFGNVRI GGLSGIYNER HYRSGHFERP PYNESTIRSV
160 170 180 190 200
YHVRDYDVQK LMQLEEPLDI FLSHDWPVGI TDYGDSESLM RQKPYFRQEI
210 220 230 240 250
EEKTLGSKPA ALLLEKLKPQ YWFSAHLHCK FAAAVQHGND GSVTKFLALD
260 270 280 290 300
KCLPGKKFLQ IIEIESEPGP FEVLYDEEWL AITRKFNSIF PLTRRYTNVS
310 320 330 340 350
TAGTIQESRE WVRKKLEERQ FKPFEFARTV PAYNPSQRVF DSIPEIPQNP
360 370 380 390 400
QTLSLLELLG LPYLLDSSPV TGERTDIPAS LAPSDLPTYD SEEIPIDDID
410
EIEEMEEAKA DDHTRDDA
Length:418
Mass (Da):48,175
Last modified:December 1, 2001 - v1
Checksum:iBE8AD41372C9A390
GO

Sequence cautioni

The sequence CAA19746.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence CAB79895.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL031004 Genomic DNA. Translation: CAA19746.1. Sequence problems.
AL161579 Genomic DNA. Translation: CAB79895.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE85955.1.
AF370499 mRNA. Translation: AAK43876.1.
PIRiT05093.
RefSeqiNP_567881.1. NM_119326.3.
UniGeneiAt.2351.

Genome annotation databases

EnsemblPlantsiAT4G31770.1; AT4G31770.1; AT4G31770.
GeneIDi829305.
GrameneiAT4G31770.1; AT4G31770.1; AT4G31770.
KEGGiath:AT4G31770.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL031004 Genomic DNA. Translation: CAA19746.1. Sequence problems.
AL161579 Genomic DNA. Translation: CAB79895.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE85955.1.
AF370499 mRNA. Translation: AAK43876.1.
PIRiT05093.
RefSeqiNP_567881.1. NM_119326.3.
UniGeneiAt.2351.

3D structure databases

ProteinModelPortaliQ94K01.
SMRiQ94K01. Positions 3-251.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT4G31770.1.

Proteomic databases

PaxDbiQ94K01.
PRIDEiQ94K01.

Protocols and materials databases

DNASUi829305.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G31770.1; AT4G31770.1; AT4G31770.
GeneIDi829305.
GrameneiAT4G31770.1; AT4G31770.1; AT4G31770.
KEGGiath:AT4G31770.

Organism-specific databases

TAIRiAT4G31770.

Phylogenomic databases

eggNOGiKOG2863. Eukaryota.
ENOG410XP0F. LUCA.
HOGENOMiHOG000216468.
InParanoidiQ94K01.
KOiK18328.
OMAiEYGNWQK.
PhylomeDBiQ94K01.

Miscellaneous databases

PROiQ94K01.

Gene expression databases

ExpressionAtlasiQ94K01. baseline and differential.
GenevisibleiQ94K01. AT.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR007708. DBR1_C.
IPR029052. Metallo-depent_PP-like.
[Graphical view]
PfamiPF05011. DBR1. 1 hit.
PF00149. Metallophos. 1 hit.
[Graphical view]
SMARTiSM01124. DBR1. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
    Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
    , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
    Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "An Arabidopsis RNA lariat debranching enzyme is essential for embryogenesis."
    Wang H., Hill K., Perry S.E.
    J. Biol. Chem. 279:1468-1473(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiDBR1_ARATH
AccessioniPrimary (citable) accession number: Q94K01
Secondary accession number(s): O81771
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2006
Last sequence update: December 1, 2001
Last modified: July 6, 2016
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.