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Protein

Deoxyhypusine hydroxylase

Gene

At3g58180

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the hydroxylation of the N(6)-(4-aminobutyl)-L-lysine intermediate to form hypusine, an essential post-translational modification only found in mature eIF-5A factor.UniRule annotation

Catalytic activityi

[eIF5A]-deoxyhypusine + AH2 + O2 = [eIF5A]-hypusine + A + H2O.UniRule annotation

Cofactori

Fe2+UniRule annotationNote: Binds 2 Fe2+ ions per subunit.UniRule annotation

Pathwayi: eIF5A hypusination

This protein is involved in the pathway eIF5A hypusination, which is part of Protein modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway eIF5A hypusination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi63Iron 1UniRule annotation1
Metal bindingi64Iron 1UniRule annotation1
Metal bindingi96Iron 1UniRule annotation1
Metal bindingi97Iron 1UniRule annotation1
Metal bindingi221Iron 2UniRule annotation1
Metal bindingi222Iron 2UniRule annotation1
Metal bindingi254Iron 2UniRule annotation1
Metal bindingi255Iron 2UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Hypusine biosynthesis

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT3G58180-MONOMER.
ReactomeiR-ATH-204626. Hypusine synthesis from eIF5A-lysine.
UniPathwayiUPA00354.

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxyhypusine hydroxylaseUniRule annotation (EC:1.14.99.29UniRule annotation)
Short name:
DOHHUniRule annotation
Alternative name(s):
Deoxyhypusine dioxygenaseUniRule annotation
Deoxyhypusine monooxygenaseUniRule annotation
Gene namesi
Ordered Locus Names:At3g58180
ORF Names:F9D24.90
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G58180.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: TAIR
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002485841 – 314Deoxyhypusine hydroxylaseAdd BLAST314

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ94JW0.

PTM databases

iPTMnetiQ94JW0.

Expressioni

Gene expression databases

ExpressionAtlasiQ94JW0. baseline and differential.
GenevisibleiQ94JW0. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT3G58180.1.

Structurei

3D structure databases

ProteinModelPortaliQ94JW0.
SMRiQ94JW0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati61 – 87HEAT-like PBS-type 1Add BLAST27
Repeati94 – 120HEAT-like PBS-type 2Add BLAST27
Repeati188 – 214HEAT-like PBS-type 3Add BLAST27
Repeati219 – 245HEAT-like PBS-type 4Add BLAST27
Repeati252 – 278HEAT-like PBS-type 5Add BLAST27

Sequence similaritiesi

Belongs to the deoxyhypusine hydroxylase family.UniRule annotation
Contains 5 HEAT-like PBS-type repeats.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0567. Eukaryota.
COG1413. LUCA.
HOGENOMiHOG000248665.
InParanoidiQ94JW0.
KOiK06072.
OMAiAIDMWEY.
OrthoDBiEOG09360HJ6.
PhylomeDBiQ94JW0.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
HAMAPiMF_03101. Deoxyhypusine_hydroxylase. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR027517. Deoxyhypusine_hydroxylase.
IPR004155. PBS_lyase_HEAT.
[Graphical view]
SMARTiSM00567. EZ_HEAT. 6 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.

Sequencei

Sequence statusi: Complete.

Q94JW0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESNGSVSSM VNLEKFLCER LVDQSQPISE RFRALFSLRN LKGPGPRNAL
60 70 80 90 100
ILASRDSSNL LAHEAAFALG QMQDAEAIPA LESVLNDMSL HPIVRHEAAE
110 120 130 140 150
ALGAIGLAGN VNILKKSLSS DPAQEVRETC ELALKRIEDM SNVDAENQSS
160 170 180 190 200
TTEKSPFMSV DPAGPAASFS SVHQLRQVLL DETKGMYERY AALFALRNHG
210 220 230 240 250
GEEAVSAIVD SLSASSALLR HEVAYVLGQL QSKTALATLS KVLRDVNEHP
260 270 280 290 300
MVRHEAAEAL GSIADEQSIA LLEEFSKDPE PIVAQSCEVA LSMLEFENSG
310
KSFEFFFTQD PLVH
Length:314
Mass (Da):34,092
Last modified:December 1, 2001 - v1
Checksum:i1971589073261ED6
GO

Sequence cautioni

The sequence CAB68156 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL137081 Genomic DNA. Translation: CAB68156.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE79751.1.
AF372878 mRNA. Translation: AAK49594.1.
AY142036 mRNA. Translation: AAM98300.1.
PIRiT45978.
RefSeqiNP_567062.1. NM_115680.3.
UniGeneiAt.19133.

Genome annotation databases

EnsemblPlantsiAT3G58180.1; AT3G58180.1; AT3G58180.
GeneIDi824987.
GrameneiAT3G58180.1; AT3G58180.1; AT3G58180.
KEGGiath:AT3G58180.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL137081 Genomic DNA. Translation: CAB68156.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE79751.1.
AF372878 mRNA. Translation: AAK49594.1.
AY142036 mRNA. Translation: AAM98300.1.
PIRiT45978.
RefSeqiNP_567062.1. NM_115680.3.
UniGeneiAt.19133.

3D structure databases

ProteinModelPortaliQ94JW0.
SMRiQ94JW0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT3G58180.1.

PTM databases

iPTMnetiQ94JW0.

Proteomic databases

PaxDbiQ94JW0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G58180.1; AT3G58180.1; AT3G58180.
GeneIDi824987.
GrameneiAT3G58180.1; AT3G58180.1; AT3G58180.
KEGGiath:AT3G58180.

Organism-specific databases

TAIRiAT3G58180.

Phylogenomic databases

eggNOGiKOG0567. Eukaryota.
COG1413. LUCA.
HOGENOMiHOG000248665.
InParanoidiQ94JW0.
KOiK06072.
OMAiAIDMWEY.
OrthoDBiEOG09360HJ6.
PhylomeDBiQ94JW0.

Enzyme and pathway databases

UniPathwayiUPA00354.
BioCyciARA:AT3G58180-MONOMER.
ReactomeiR-ATH-204626. Hypusine synthesis from eIF5A-lysine.

Miscellaneous databases

PROiQ94JW0.

Gene expression databases

ExpressionAtlasiQ94JW0. baseline and differential.
GenevisibleiQ94JW0. AT.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
HAMAPiMF_03101. Deoxyhypusine_hydroxylase. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR027517. Deoxyhypusine_hydroxylase.
IPR004155. PBS_lyase_HEAT.
[Graphical view]
SMARTiSM00567. EZ_HEAT. 6 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiDOHH_ARATH
AccessioniPrimary (citable) accession number: Q94JW0
Secondary accession number(s): Q9M2J8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: December 1, 2001
Last modified: November 30, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.