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Q94JW0 (DOHH_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Deoxyhypusine hydroxylase
Short name=DOHH
EC=1.14.99.29
Alternative name(s):
Deoxyhypusine dioxygenase
Deoxyhypusine monooxygenase
Gene names
Ordered Locus Names:At3g58180
ORF Names:F9D24.90
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length314 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the hydroxylation of the N(6)-(4-aminobutyl)-L-lysine intermediate to form hypusine, an essential post-translational modification only found in mature eIF-5A factor By similarity. HAMAP-Rule MF_03101

Catalytic activity

Protein N(6)-(4-aminobutyl)-L-lysine + AH2 + O2 = protein N(6)-((R)-4-amino-2-hydroxybutyl)-L-lysine + A + H2O. HAMAP-Rule MF_03101

Cofactor

Binds 2 Fe2+ ions per subunit By similarity.

Pathway

Protein modification; eIF5A hypusination. HAMAP-Rule MF_03101

Sequence similarities

Belongs to the deoxyhypusine hydroxylase family.

Contains 5 HEAT-like PBS-type repeats.

Sequence caution

The sequence CAB68156.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
   Biological processHypusine biosynthesis
   DomainRepeat
   LigandIron
Metal-binding
   Molecular functionMonooxygenase
Oxidoreductase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processpeptidyl-lysine modification to hypusine

Inferred from sequence or structural similarity. Source: UniProtKB

   Cellular_componentcytosol

Inferred from direct assay PubMed 21166475. Source: TAIR

   Molecular_functiondeoxyhypusine monooxygenase activity

Inferred from sequence or structural similarity. Source: UniProtKB

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 314314Deoxyhypusine hydroxylase HAMAP-Rule MF_03101
PRO_0000248584

Regions

Repeat61 – 8727HEAT-like PBS-type 1 HAMAP-Rule MF_03101
Repeat94 – 12027HEAT-like PBS-type 2 HAMAP-Rule MF_03101
Repeat188 – 21427HEAT-like PBS-type 3 HAMAP-Rule MF_03101
Repeat219 – 24527HEAT-like PBS-type 4 HAMAP-Rule MF_03101
Repeat252 – 27827HEAT-like PBS-type 5 HAMAP-Rule MF_03101

Sites

Metal binding631Iron 1 By similarity
Metal binding641Iron 1 By similarity
Metal binding961Iron 1 By similarity
Metal binding971Iron 1 By similarity
Metal binding2211Iron 2 By similarity
Metal binding2221Iron 2 By similarity
Metal binding2541Iron 2 By similarity
Metal binding2551Iron 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q94JW0 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: 1971589073261ED6

FASTA31434,092
        10         20         30         40         50         60 
MESNGSVSSM VNLEKFLCER LVDQSQPISE RFRALFSLRN LKGPGPRNAL ILASRDSSNL 

        70         80         90        100        110        120 
LAHEAAFALG QMQDAEAIPA LESVLNDMSL HPIVRHEAAE ALGAIGLAGN VNILKKSLSS 

       130        140        150        160        170        180 
DPAQEVRETC ELALKRIEDM SNVDAENQSS TTEKSPFMSV DPAGPAASFS SVHQLRQVLL 

       190        200        210        220        230        240 
DETKGMYERY AALFALRNHG GEEAVSAIVD SLSASSALLR HEVAYVLGQL QSKTALATLS 

       250        260        270        280        290        300 
KVLRDVNEHP MVRHEAAEAL GSIADEQSIA LLEEFSKDPE PIVAQSCEVA LSMLEFENSG 

       310 
KSFEFFFTQD PLVH

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. expand/collapse author list , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AL137081 Genomic DNA. Translation: CAB68156.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE79751.1.
AF372878 mRNA. Translation: AAK49594.1.
AY142036 mRNA. Translation: AAM98300.1.
IPIIPI00521283.
PIRT45978.
RefSeqNP_567062.1. NM_115680.2.
UniGeneAt.19133.

3D structure databases

ProteinModelPortalQ94JW0.
SMRQ94JW0. Positions 76-105, 121-291.
ModBaseSearch...

Proteomic databases

PaxDbQ94JW0.
PRIDEQ94JW0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT3G58180.1; AT3G58180.1; AT3G58180.
GeneID824987.
KEGGath:AT3G58180.

Organism-specific databases

TAIRAt3g58180.

Phylogenomic databases

eggNOGCOG1413.
HOGENOMHOG000248665.
InParanoidQ94JW0.
KOK06072.
OMAFRHEVAF.
PhylomeDBQ94JW0.
ProtClustDBCLSN2689216.

Enzyme and pathway databases

UniPathwayUPA00354.

Gene expression databases

ArrayExpressQ94JW0.
GenevestigatorQ94JW0.
GermOnlineAT3G58180. Arabidopsis thaliana.

Family and domain databases

Gene3D1.25.10.10. 1 hit.
HAMAPMF_03101. Deoxyhypusine_hydroxylase.
InterProIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR004155. PBS_lyase_HEAT.
[Graphical view]
SMARTSM00567. EZ_HEAT. 6 hits.
[Graphical view]
SUPFAMSSF48371. ARM-type_fold. 1 hit.
PROSITEPS50077. HEAT_REPEAT. False negative.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDOHH_ARATH
AccessionPrimary (citable) accession number: Q94JW0
Secondary accession number(s): Q9M2J8
Entry history
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: December 1, 2001
Last modified: May 1, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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