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Q94JW0

- DOHH_ARATH

UniProt

Q94JW0 - DOHH_ARATH

Protein

Deoxyhypusine hydroxylase

Gene

At3g58180

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 95 (01 Oct 2014)
      Sequence version 1 (01 Dec 2001)
      Previous versions | rss
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    Functioni

    Catalyzes the hydroxylation of the N(6)-(4-aminobutyl)-L-lysine intermediate to form hypusine, an essential post-translational modification only found in mature eIF-5A factor.UniRule annotation

    Catalytic activityi

    [eIF5A]-deoxyhypusine + AH2 + O2 = [eIF5A]-hypusine + A + H2O.UniRule annotation

    Cofactori

    Binds 2 Fe2+ ions per subunit.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi63 – 631Iron 1UniRule annotation
    Metal bindingi64 – 641Iron 1UniRule annotation
    Metal bindingi96 – 961Iron 1UniRule annotation
    Metal bindingi97 – 971Iron 1UniRule annotation
    Metal bindingi221 – 2211Iron 2UniRule annotation
    Metal bindingi222 – 2221Iron 2UniRule annotation
    Metal bindingi254 – 2541Iron 2UniRule annotation
    Metal bindingi255 – 2551Iron 2UniRule annotation

    GO - Molecular functioni

    1. deoxyhypusine monooxygenase activity Source: UniProtKB
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. peptidyl-lysine modification to peptidyl-hypusine Source: UniProtKB

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Biological processi

    Hypusine biosynthesis

    Keywords - Ligandi

    Iron, Metal-binding

    Enzyme and pathway databases

    BioCyciARA:AT3G58180-MONOMER.
    UniPathwayiUPA00354.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Deoxyhypusine hydroxylaseUniRule annotation (EC:1.14.99.29UniRule annotation)
    Short name:
    DOHHUniRule annotation
    Alternative name(s):
    Deoxyhypusine dioxygenaseUniRule annotation
    Deoxyhypusine monooxygenaseUniRule annotation
    Gene namesi
    Ordered Locus Names:At3g58180
    ORF Names:F9D24.90
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 3

    Organism-specific databases

    TAIRiAT3G58180.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: TAIR

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 314314Deoxyhypusine hydroxylasePRO_0000248584Add
    BLAST

    Proteomic databases

    PaxDbiQ94JW0.
    PRIDEiQ94JW0.

    Expressioni

    Gene expression databases

    ArrayExpressiQ94JW0.
    GenevestigatoriQ94JW0.

    Structurei

    3D structure databases

    ProteinModelPortaliQ94JW0.
    SMRiQ94JW0. Positions 121-291.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati61 – 8727HEAT-like PBS-type 1Add
    BLAST
    Repeati94 – 12027HEAT-like PBS-type 2Add
    BLAST
    Repeati188 – 21427HEAT-like PBS-type 3Add
    BLAST
    Repeati219 – 24527HEAT-like PBS-type 4Add
    BLAST
    Repeati252 – 27827HEAT-like PBS-type 5Add
    BLAST

    Sequence similaritiesi

    Belongs to the deoxyhypusine hydroxylase family.UniRule annotation
    Contains 5 HEAT-like PBS-type repeats.UniRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG1413.
    HOGENOMiHOG000248665.
    InParanoidiQ94JW0.
    KOiK06072.
    OMAiRESCQVA.
    PhylomeDBiQ94JW0.

    Family and domain databases

    Gene3Di1.25.10.10. 1 hit.
    HAMAPiMF_03101. Deoxyhypusine_hydroxylase.
    InterProiIPR011989. ARM-like.
    IPR016024. ARM-type_fold.
    IPR027517. Deoxyhypusine_hydroxylase.
    IPR004155. PBS_lyase_HEAT.
    [Graphical view]
    PfamiPF03130. HEAT_PBS. 1 hit.
    [Graphical view]
    SMARTiSM00567. EZ_HEAT. 6 hits.
    [Graphical view]
    SUPFAMiSSF48371. SSF48371. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q94JW0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MESNGSVSSM VNLEKFLCER LVDQSQPISE RFRALFSLRN LKGPGPRNAL    50
    ILASRDSSNL LAHEAAFALG QMQDAEAIPA LESVLNDMSL HPIVRHEAAE 100
    ALGAIGLAGN VNILKKSLSS DPAQEVRETC ELALKRIEDM SNVDAENQSS 150
    TTEKSPFMSV DPAGPAASFS SVHQLRQVLL DETKGMYERY AALFALRNHG 200
    GEEAVSAIVD SLSASSALLR HEVAYVLGQL QSKTALATLS KVLRDVNEHP 250
    MVRHEAAEAL GSIADEQSIA LLEEFSKDPE PIVAQSCEVA LSMLEFENSG 300
    KSFEFFFTQD PLVH 314
    Length:314
    Mass (Da):34,092
    Last modified:December 1, 2001 - v1
    Checksum:i1971589073261ED6
    GO

    Sequence cautioni

    The sequence CAB68156.1 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL137081 Genomic DNA. Translation: CAB68156.1. Sequence problems.
    CP002686 Genomic DNA. Translation: AEE79751.1.
    AF372878 mRNA. Translation: AAK49594.1.
    AY142036 mRNA. Translation: AAM98300.1.
    PIRiT45978.
    RefSeqiNP_567062.1. NM_115680.2.
    UniGeneiAt.19133.

    Genome annotation databases

    EnsemblPlantsiAT3G58180.1; AT3G58180.1; AT3G58180.
    GeneIDi824987.
    KEGGiath:AT3G58180.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL137081 Genomic DNA. Translation: CAB68156.1 . Sequence problems.
    CP002686 Genomic DNA. Translation: AEE79751.1 .
    AF372878 mRNA. Translation: AAK49594.1 .
    AY142036 mRNA. Translation: AAM98300.1 .
    PIRi T45978.
    RefSeqi NP_567062.1. NM_115680.2.
    UniGenei At.19133.

    3D structure databases

    ProteinModelPortali Q94JW0.
    SMRi Q94JW0. Positions 121-291.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PaxDbi Q94JW0.
    PRIDEi Q94JW0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT3G58180.1 ; AT3G58180.1 ; AT3G58180 .
    GeneIDi 824987.
    KEGGi ath:AT3G58180.

    Organism-specific databases

    TAIRi AT3G58180.

    Phylogenomic databases

    eggNOGi COG1413.
    HOGENOMi HOG000248665.
    InParanoidi Q94JW0.
    KOi K06072.
    OMAi RESCQVA.
    PhylomeDBi Q94JW0.

    Enzyme and pathway databases

    UniPathwayi UPA00354 .
    BioCyci ARA:AT3G58180-MONOMER.

    Miscellaneous databases

    PROi Q94JW0.

    Gene expression databases

    ArrayExpressi Q94JW0.
    Genevestigatori Q94JW0.

    Family and domain databases

    Gene3Di 1.25.10.10. 1 hit.
    HAMAPi MF_03101. Deoxyhypusine_hydroxylase.
    InterProi IPR011989. ARM-like.
    IPR016024. ARM-type_fold.
    IPR027517. Deoxyhypusine_hydroxylase.
    IPR004155. PBS_lyase_HEAT.
    [Graphical view ]
    Pfami PF03130. HEAT_PBS. 1 hit.
    [Graphical view ]
    SMARTi SM00567. EZ_HEAT. 6 hits.
    [Graphical view ]
    SUPFAMi SSF48371. SSF48371. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
      Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
      , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
      Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
      Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
      Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiDOHH_ARATH
    AccessioniPrimary (citable) accession number: Q94JW0
    Secondary accession number(s): Q9M2J8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 5, 2006
    Last sequence update: December 1, 2001
    Last modified: October 1, 2014
    This is version 95 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3