Q94JU3 (CSN7_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
COP9 signalosome complex subunit 7
Short name=CSN complex subunit 7

Alternative name(s):
Protein FUSCA 5

Gene names

Name:	CSN7
Synonyms:	FUS5
Ordered Locus Names:	At1g02090
ORF Names:	T7I23.24, T7I23.25

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	260 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes such as photomorphogenesis and auxin and jasmonate responses. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF. It is involved in repression of photomorphogenesis in darkness by regulating the activity of COP1-containing Ubl ligase complexes. The complex is also required for degradation of IAA6 by regulating the activity of the Ubl ligase SCF-TIR complex. Ref.8
Subunit structure	Component of the CSN complex, probably composed of CSN1, CSN2, CSN3, CSN4, CSN5 (CSN5A or CSN5B), CSN6 (CSN6A or CSN6B), CSN7 and CSN8. In the CSN complex, it probably interacts directly with CSN1, CSN4, CSN6 and CSN8. Also exists as a monomeric form. Interacts with EIF3S6/INT-6. Ref.1 Ref.7 Ref.9
Subcellular location	Cytoplasm. Nucleus.
Post-translational modification	Phosphorylated. Ref.1
Sequence similarities	Belongs to the CSN7/EIF3M family. CSN7 subfamily. Contains 1 PCI domain.

Ontologies

Keywords
Biological process	Phytochrome signaling pathway
Cellular component	Cytoplasm Nucleus Signalosome
Coding sequence diversity	Alternative splicing
Molecular function	Developmental protein
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cullin deneddylation Inferred from mutant phenotype. Source: TAIR multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW red, far-red light phototransduction Inferred from electronic annotation. Source: UniProtKB-KW signalosome assembly Inferred from mutant phenotype. Source: TAIR
Cellular component	cytosol Inferred from direct assay. Source: TAIR signalosome Inferred from physical interaction. Source: TAIR
Molecular function	protein binding Inferred from physical interaction Ref.1Ref.7Ref.9. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	Q9M4T7	6	EBI-531152,EBI-1635572
CSN1	P45432	3	EBI-531152,EBI-530996
CSN4	Q8L5U0	5	EBI-531152,EBI-531074
CSN8	P43255	3	EBI-531152,EBI-530981

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q94JU3-1)

Also known as: CSN7ii;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q94JU3-2)

Also known as: CSN7i;

The sequence of this isoform differs from the canonical sequence as follows:
     224-260: GDVDIRGNKEMFGEPSGVMDYEEDGIRPKRRRHPVTR → RE

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 260

260

COP9 signalosome complex subunit 7

PRO_0000121003

Regions

Domain

60 – 156

97

PCI

Natural variations

Alternative sequence

224 – 260

37

GDVDI…HPVTR → RE in isoform 2.

VSP_011914

Secondary structure

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260

Helix Strand Turn

Details...

Helix

7 – 18

12

Helix

23 – 25

3

Helix

29 – 35

7

Helix

43 – 46

4

Helix

49 – 52

4

Helix

60 – 70

11

Helix

77 – 80

4

Helix

81 – 83

3

Helix

89 – 106

18

Beta strand

108 – 111

4

Helix

112 – 119

8

Helix

124 – 133

10

Turn

134 – 137

4

Beta strand

144 – 146

3

Turn

147 – 150

4

Beta strand

151 – 157

7

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (CSN7ii) [UniParc]. Last modified December 1, 2001. Version 1. Checksum: CBBDA06097E44689	FASTA	260	29,469
	10 20 30 40 50 60 MDIEQKQAEI IDQLVKRAST CKSEALGPLI IEATSHPSLF AFSEILALPN VAQLEGTTDS 70 80 90 100 110 120 VYLDLLRLFA HGTWGDYKCN ATRLPHLSPD QILKLKQLTV LTLAESNKVL PYDTLMVELD 130 140 150 160 170 180 VSNVRELEDF LINECMYAGI VRGKLDQLKR CFEVPFAAGR DLRPGQLGNM LHTLSNWLNT 190 200 210 220 230 240 SENLLISIQD KIKWADNMSE MDKKHRKEAE EGVEEVKKSL SMKGDVDIRG NKEMFGEPSG 250 260 VMDYEEDGIR PKRRRHPVTR
	Isoform 2 (CSN7i) [UniParc]. Checksum: B15D2B6031AFC27D	FASTA	225	25,471
	10 20 30 40 50 60 MDIEQKQAEI IDQLVKRAST CKSEALGPLI IEATSHPSLF AFSEILALPN VAQLEGTTDS 70 80 90 100 110 120 VYLDLLRLFA HGTWGDYKCN ATRLPHLSPD QILKLKQLTV LTLAESNKVL PYDTLMVELD 130 140 150 160 170 180 VSNVRELEDF LINECMYAGI VRGKLDQLKR CFEVPFAAGR DLRPGQLGNM LHTLSNWLNT 190 200 210 220 SENLLISIQD KIKWADNMSE MDKKHRKEAE EGVEEVKKSL SMKRE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Arabidopsis FUSCA5 encodes a novel phosphoprotein that is a component of the COP9 complex." Karniol B., Malec P., Chamovitz D.A. Plant Cell 11:839-848(1999) [PubMed: 10330469] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), PHOSPHORYLATION, SUBUNIT.
[2]	"Subunit interaction maps for the regulatory particle of the 26S proteasome and the COP9 signalosome." Fu H., Reis N., Lee Y., Glickman M.H., Vierstra R. EMBO J. 20:7096-7107(2001) [PubMed: 11742986] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2). Strain: cv. Columbia.
[3]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[5]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: cv. Columbia.
[6]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[7]	"Arabidopsis eIF3e (INT-6) associates with both eIF3c and the COP9 signalosome subunit CSN7." Yahalom A., Kim T.-H., Winter E., Karniol B., von Arnim A.G., Chamovitz D.A. J. Biol. Chem. 276:334-340(2001) [PubMed: 11029466] [Abstract] Cited for: INTERACTION WITH EIF3S6.
[8]	"Interactions of the COP9 signalosome with the E3 ubiquitin ligase SCF(TIR1) in mediating auxin response." Schwechheimer C., Serino G., Callis J., Crosby W.L., Lyapina S., Deshaies R.J., Gray W.M., Estelle M., Deng X.-W. Science 292:1379-1382(2001) [PubMed: 11337587] [Abstract] Cited for: FUNCTION.
[9]	"Characterization of the last subunit of the Arabidopsis COP9 signalosome: implications for the overall structure and origin of the complex." Serino G., Su H., Peng Z., Tsuge T., Wei N., Gu H., Deng X.-W. Plant Cell 15:719-731(2003) [PubMed: 12615944] [Abstract] Cited for: INTERACTION WITH CSN1; CSN4; CSN6 AND CSN8.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF063852 mRNA. Translation: AAC25563.1.
AF395065 mRNA. Translation: AAL58108.1.
AF395066 mRNA. Translation: AAL58109.1.
U89959 Genomic DNA. No translation available.
CP002684 Genomic DNA. Translation: AEE27379.1.
CP002684 Genomic DNA. Translation: AEE27380.1.
AF372934 mRNA. Translation: AAK50074.1.
AY133518 mRNA. Translation: AAM91348.1.
AY087971 mRNA. Translation: AAM65518.1.

IPI

IPI00523211.
IPI00526282.

PIR

T52188.

RefSeq

NP_563645.1. NM_100089.2.
NP_849576.1. NM_179245.2.

UniGene

At.10718.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3CHM	X-ray	1.50	A	2-169	[»]

ProteinModelPortal

Q94JU3.

SMR

Q94JU3. Positions 4-164.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q94JU3. 23 interactions.

STRING

Q94JU3.

Proteomic databases

PRIDE

Q94JU3.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblPlants

AT1G02090.1; AT1G02090.1; AT1G02090.

GeneID

839241.

GenomeReviews

Gene locus AT1G02090 in contig CT485782_GR.

KEGG

ath:AT1G02090.

NMPDR

fig|3702.1.peg.322.

Organism-specific databases

GeneFarm

4561.

TAIR

At1g02090.

Phylogenomic databases

eggNOG

KOG3250.

GeneTree

EPGT00050000009211.

HOGENOM

HBG715921.

InParanoid

Q94JU3.

OMA

VMDYEED.

PhylomeDB

Q94JU3.

ProtClustDB

CLSN2687617.

Gene expression databases

ArrayExpress

Q94JU3.

Genevestigator

Q94JU3.

Family and domain databases

InterPro

IPR000717. PCI_dom.
[Graphical view]

KO

K12180.

Pfam

PF01399. PCI. 1 hit.
[Graphical view]

SMART

SM00088. PINT. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

CSN7_ARATH

Accession

Primary (citable) accession number: Q94JU3
Secondary accession number(s): O81247

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 23, 2004
Last sequence update:	December 1, 2001
Last modified:	December 14, 2011
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program