Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Auxin response factor 2

Gene

ARF2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Auxin response factors (ARFs) are transcriptional factors that binds specifically to the DNA sequence 5'-TGTCTC-3' found in the auxin-responsive promoter elements (AuxREs). Could act as transcriptional activator or repressor. Formation of heterodimers with Aux/IAA proteins may alter their ability to modulate early auxin response genes expression. Promotes flowering, stamen development, floral organ abscission and fruit dehiscence. Functions independently of ethylene and cytokinin response pathways. May act as a repressor of cell division and organ growth.4 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi164 – 266103TF-B3PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • sequence-specific DNA binding Source: TAIR
  • transcription factor activity, sequence-specific DNA binding Source: TAIR

GO - Biological processi

  • auxin-activated signaling pathway Source: UniProtKB-KW
  • floral organ abscission Source: TAIR
  • fruit dehiscence Source: TAIR
  • leaf senescence Source: TAIR
  • negative regulation of cell proliferation Source: TAIR
  • negative regulation of transcription, DNA-templated Source: TAIR
  • plant ovule development Source: TAIR
  • positive regulation of flower development Source: TAIR
  • response to abscisic acid Source: TAIR
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Auxin signaling pathway, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Auxin response factor 2
Alternative name(s):
ARF1-binding protein
Short name:
ARF1-BP
Protein MEGAINTEGUMENTA
Gene namesi
Name:ARF2
Synonyms:MNT
Ordered Locus Names:At5g62000
ORF Names:MTG10.3
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G62000.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Large, dark green rosette leaves, delayed flowering, thick and long inflorescence, abnormal flower morphology and sterility in early formed flowers, but fertility in late-formed flowers. Delayed senescence and abscission. Increased seed size and weight, and extra cell division and expansion in many organs.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 859859Auxin response factor 2PRO_0000111506Add
BLAST

Proteomic databases

PaxDbiQ94JM3.
PRIDEiQ94JM3.

PTM databases

iPTMnetiQ94JM3.

Expressioni

Tissue specificityi

Expressed in the whole plant.2 Publications

Developmental stagei

Expressed in the sepals and carpels of young flower buds. At stage 10 of flower development, expression in the carpels becomes restricted to the style. Also expressed in anthers and filaments. At stage 13, expressed in the region at the top of the pedicel, including the abscission zone. Expressed in developing siliques.2 Publications

Gene expression databases

ExpressionAtlasiQ94JM3. baseline and differential.
GenevisibleiQ94JM3. AT.

Interactioni

Subunit structurei

Homodimers and heterodimers. Interacts with ARF1.

Binary interactionsi

WithEntry#Exp.IntActNotes
ASK7Q390113EBI-1799262,EBI-1798250

Protein-protein interaction databases

BioGridi21565. 11 interactions.
DIPiDIP-46014N.
IntActiQ94JM3. 5 interactions.
STRINGi3702.AT5G62000.1.

Structurei

3D structure databases

ProteinModelPortaliQ94JM3.
SMRiQ94JM3. Positions 57-394, 733-821.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini733 – 81785PB1PROSITE-ProRule annotationAdd
BLAST

Domaini

Interactions between auxin response factors (ARFs) and Aux/IAA proteins occur through their C-terminal dimerization domains III and IV.

Sequence similaritiesi

Belongs to the ARF family.Curated
Contains 1 PB1 domain.PROSITE-ProRule annotation
Contains 1 TF-B3 DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IE1Z. Eukaryota.
ENOG4110RNJ. LUCA.
InParanoidiQ94JM3.
OMAiDEENDMM.
PhylomeDBiQ94JM3.

Family and domain databases

Gene3Di2.40.330.10. 1 hit.
InterProiIPR033389. AUX/IAA_dom.
IPR010525. Auxin_resp.
IPR003340. B3_DNA-bd.
IPR015300. DNA-bd_pseudobarrel.
IPR000270. PB1_dom.
[Graphical view]
PfamiPF02309. AUX_IAA. 2 hits.
PF06507. Auxin_resp. 1 hit.
PF02362. B3. 1 hit.
[Graphical view]
SMARTiSM01019. B3. 1 hit.
[Graphical view]
SUPFAMiSSF101936. SSF101936. 1 hit.
PROSITEiPS50863. B3. 1 hit.
PS51745. PB1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q94JM3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASSEVSMKG NRGGDNFSSS GFSDPKETRN VSVAGEGQKS NSTRSAAAER
60 70 80 90 100
ALDPEAALYR ELWHACAGPL VTVPRQDDRV FYFPQGHIEQ VEASTNQAAE
110 120 130 140 150
QQMPLYDLPS KLLCRVINVD LKAEADTDEV YAQITLLPEA NQDENAIEKE
160 170 180 190 200
APLPPPPRFQ VHSFCKTLTA SDTSTHGGFS VLRRHADECL PPLDMSRQPP
210 220 230 240 250
TQELVAKDLH ANEWRFRHIF RGQPRRHLLQ SGWSVFVSSK RLVAGDAFIF
260 270 280 290 300
LRGENGELRV GVRRAMRQQG NVPSSVISSH SMHLGVLATA WHAISTGTMF
310 320 330 340 350
TVYYKPRTSP SEFIVPFDQY MESVKNNYSI GMRFKMRFEG EEAPEQRFTG
360 370 380 390 400
TIVGIEESDP TRWPKSKWRS LKVRWDETSS IPRPDRVSPW KVEPALAPPA
410 420 430 440 450
LSPVPMPRPK RPRSNIAPSS PDSSMLTREG TTKANMDPLP ASGLSRVLQG
460 470 480 490 500
QEYSTLRTKH TESVECDAPE NSVVWQSSAD DDKVDVVSGS RRYGSENWMS
510 520 530 540 550
SARHEPTYTD LLSGFGTNID PSHGQRIPFY DHSSSPSMPA KRILSDSEGK
560 570 580 590 600
FDYLANQWQM IHSGLSLKLH ESPKVPAATD ASLQGRCNVK YSEYPVLNGL
610 620 630 640 650
STENAGGNWP IRPRALNYYE EVVNAQAQAQ AREQVTKQPF TIQEETAKSR
660 670 680 690 700
EGNCRLFGIP LTNNMNGTDS TMSQRNNLND AAGLTQIASP KVQDLSDQSK
710 720 730 740 750
GSKSTNDHRE QGRPFQTNNP HPKDAQTKTN SSRSCTKVHK QGIALGRSVD
760 770 780 790 800
LSKFQNYEEL VAELDRLFEF NGELMAPKKD WLIVYTDEEN DMMLVGDDPW
810 820 830 840 850
QEFCCMVRKI FIYTKEEVRK MNPGTLSCRS EEEAVVGEGS DAKDAKSASN

PSLSSAGNS
Length:859
Mass (Da):95,701
Last modified:April 13, 2004 - v2
Checksum:i6B75171D7FF0E66B
GO

Sequence cautioni

The sequence AAC49752.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence AAK55665.1 differs from that shown. Reason: Frameshift at position 657. Curated
The sequence AAN46837.1 differs from that shown. Reason: Frameshift at position 657. Curated
The sequence CAD29696.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence CAD30210.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti491 – 4922RR → KK in AAC49752 (PubMed:9188533).Curated
Sequence conflicti491 – 4922RR → KK in CAD29696 (Ref. 7) Curated
Sequence conflicti491 – 4922RR → KK in CAD30210 (Ref. 7) Curated
Sequence conflicti520 – 5201D → E in AAC49752 (PubMed:9188533).Curated
Sequence conflicti520 – 5201D → E in CAD29696 (Ref. 7) Curated
Sequence conflicti520 – 5201D → E in CAD30210 (Ref. 7) Curated
Sequence conflicti596 – 5961V → A in BAD93968 (Ref. 5) Curated
Sequence conflicti675 – 6751R → K in AAC49752 (PubMed:9188533).Curated
Sequence conflicti675 – 6751R → K in CAD29696 (Ref. 7) Curated
Sequence conflicti675 – 6751R → K in CAD30210 (Ref. 7) Curated
Sequence conflicti823 – 8231P → Q in AAC49752 (PubMed:9188533).Curated
Sequence conflicti823 – 8231P → Q in CAD29696 (Ref. 7) Curated
Sequence conflicti823 – 8231P → Q in CAD30210 (Ref. 7) Curated
Sequence conflicti826 – 8261L → V in BAD93959 (Ref. 5) Curated
Sequence conflicti826 – 8261L → V in BAD93897 (Ref. 5) Curated
Sequence conflicti826 – 8261L → V in BAD93891 (Ref. 5) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY669787 mRNA. Translation: AAT67071.1.
AB016880 Genomic DNA. Translation: BAB10162.1.
CP002688 Genomic DNA. Translation: AED97548.1.
CP002688 Genomic DNA. Translation: AED97549.1.
CP002688 Genomic DNA. Translation: AED97550.1.
AF336918 mRNA. Translation: AAG53999.1.
AF378862 mRNA. Translation: AAK55665.1. Frameshift.
BT000784 mRNA. Translation: AAN31923.1.
BT001072 mRNA. Translation: AAN46837.1. Frameshift.
AK221252 mRNA. Translation: BAD93891.1.
AK221254 mRNA. Translation: BAD93897.1.
AK221274 mRNA. Translation: BAD93959.1.
AK221277 mRNA. Translation: BAD93968.1.
AK221282 mRNA. Translation: BAD93985.1.
AK221305 mRNA. Translation: BAD94058.1.
U89771 mRNA. Translation: AAC49752.1. Different initiation.
AJ441313 mRNA. Translation: CAD29696.1. Different initiation.
AJ458328 mRNA. Translation: CAD30210.1. Different initiation.
RefSeqiNP_201006.2. NM_125593.4. [Q94JM3-1]
NP_851244.1. NM_180913.3. [Q94JM3-1]
NP_974980.1. NM_203251.3. [Q94JM3-1]
UniGeneiAt.21704.

Genome annotation databases

EnsemblPlantsiAT5G62000.1; AT5G62000.1; AT5G62000. [Q94JM3-1]
AT5G62000.2; AT5G62000.2; AT5G62000. [Q94JM3-1]
AT5G62000.3; AT5G62000.3; AT5G62000. [Q94JM3-1]
GeneIDi836321.
KEGGiath:AT5G62000.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY669787 mRNA. Translation: AAT67071.1.
AB016880 Genomic DNA. Translation: BAB10162.1.
CP002688 Genomic DNA. Translation: AED97548.1.
CP002688 Genomic DNA. Translation: AED97549.1.
CP002688 Genomic DNA. Translation: AED97550.1.
AF336918 mRNA. Translation: AAG53999.1.
AF378862 mRNA. Translation: AAK55665.1. Frameshift.
BT000784 mRNA. Translation: AAN31923.1.
BT001072 mRNA. Translation: AAN46837.1. Frameshift.
AK221252 mRNA. Translation: BAD93891.1.
AK221254 mRNA. Translation: BAD93897.1.
AK221274 mRNA. Translation: BAD93959.1.
AK221277 mRNA. Translation: BAD93968.1.
AK221282 mRNA. Translation: BAD93985.1.
AK221305 mRNA. Translation: BAD94058.1.
U89771 mRNA. Translation: AAC49752.1. Different initiation.
AJ441313 mRNA. Translation: CAD29696.1. Different initiation.
AJ458328 mRNA. Translation: CAD30210.1. Different initiation.
RefSeqiNP_201006.2. NM_125593.4. [Q94JM3-1]
NP_851244.1. NM_180913.3. [Q94JM3-1]
NP_974980.1. NM_203251.3. [Q94JM3-1]
UniGeneiAt.21704.

3D structure databases

ProteinModelPortaliQ94JM3.
SMRiQ94JM3. Positions 57-394, 733-821.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi21565. 11 interactions.
DIPiDIP-46014N.
IntActiQ94JM3. 5 interactions.
STRINGi3702.AT5G62000.1.

PTM databases

iPTMnetiQ94JM3.

Proteomic databases

PaxDbiQ94JM3.
PRIDEiQ94JM3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G62000.1; AT5G62000.1; AT5G62000. [Q94JM3-1]
AT5G62000.2; AT5G62000.2; AT5G62000. [Q94JM3-1]
AT5G62000.3; AT5G62000.3; AT5G62000. [Q94JM3-1]
GeneIDi836321.
KEGGiath:AT5G62000.

Organism-specific databases

TAIRiAT5G62000.

Phylogenomic databases

eggNOGiENOG410IE1Z. Eukaryota.
ENOG4110RNJ. LUCA.
InParanoidiQ94JM3.
OMAiDEENDMM.
PhylomeDBiQ94JM3.

Miscellaneous databases

PROiQ94JM3.

Gene expression databases

ExpressionAtlasiQ94JM3. baseline and differential.
GenevisibleiQ94JM3. AT.

Family and domain databases

Gene3Di2.40.330.10. 1 hit.
InterProiIPR033389. AUX/IAA_dom.
IPR010525. Auxin_resp.
IPR003340. B3_DNA-bd.
IPR015300. DNA-bd_pseudobarrel.
IPR000270. PB1_dom.
[Graphical view]
PfamiPF02309. AUX_IAA. 2 hits.
PF06507. Auxin_resp. 1 hit.
PF02362. B3. 1 hit.
[Graphical view]
SMARTiSM01019. B3. 1 hit.
[Graphical view]
SUPFAMiSSF101936. SSF101936. 1 hit.
PROSITEiPS50863. B3. 1 hit.
PS51745. PB1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Functional genomic analysis of the AUXIN RESPONSE FACTOR gene family members in Arabidopsis thaliana: unique and overlapping functions of ARF7 and ARF19."
    Okushima Y., Overvoorde P.J., Arima K., Alonso J.M., Chan A., Chang C., Ecker J.R., Hughes B., Lui A., Nguyen D., Onodera C., Quach H., Smith A., Yu G., Theologis A.
    Plant Cell 17:444-463(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
  2. "Structural analysis of Arabidopsis thaliana chromosome 5. VII. Sequence features of the regions of 1,013,767 bp covered by sixteen physically assigned P1 and TAC clones."
    Nakamura Y., Sato S., Asamizu E., Kaneko T., Kotani H., Miyajima N., Tabata S.
    DNA Res. 5:297-308(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. "ARF1, a transcription factor that binds to auxin response elements."
    Ulmasov T., Hagen G., Guilfoyle T.J.
    Science 276:1865-1868(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 394-859.
    Strain: cv. Columbia.
  7. "Nucleotide sequence of the Arabidopsis IAA30."
    Sessa G., Carabelli M., Ciarbelli A.R., Ruzza V., Steindler C., Ruberti I.
    Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE OF 406-859.
    Strain: cv. Columbia.
  8. "Dimerization and DNA binding of auxin response factors."
    Ulmasov T., Hagen G., Guilfoyle T.J.
    Plant J. 19:309-319(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: DIMERIZATION, TISSUE SPECIFICITY.
  9. "Auxin-responsive gene expression: genes, promoters and regulatory factors."
    Hagen G., Guilfoyle T.J.
    Plant Mol. Biol. 49:373-385(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE, FUNCTION.
  10. "AUXIN RESPONSE FACTOR1 and AUXIN RESPONSE FACTOR2 regulate senescence and floral organ abscission in Arabidopsis thaliana."
    Ellis C.M., Nagpal P., Young J.C., Hagen G., Guilfoyle T.J., Reed J.W.
    Development 132:4563-4574(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DEVELOPMENTAL STAGE.
  11. "AUXIN RESPONSE FACTOR 2 (ARF2): a pleiotropic developmental regulator."
    Okushima Y., Mitina I., Quach H.L., Theologis A.
    Plant J. 43:29-46(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
  12. "The AUXIN RESPONSE FACTOR 2 gene of Arabidopsis links auxin signalling, cell division, and the size of seeds and other organs."
    Schruff M.C., Spielman M., Tiwari S., Adams S., Fenby N., Scott R.J.
    Development 133:251-261(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  13. Cited for: GENE FAMILY.

Entry informationi

Entry nameiARFB_ARATH
AccessioniPrimary (citable) accession number: Q94JM3
Secondary accession number(s): O23671
, Q56YL7, Q56YP4, Q56YR7, Q9C5W5, Q9FIT6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: April 13, 2004
Last modified: June 8, 2016
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Caution

Was originally (Ref. 5) erroneously termed IAA26 and IAA30.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.