Reviewed,
UniProtKB/Swiss-Prot Q94JM3 (ARFB_ARATH)
Last modified
June 16, 2009.
Version 56.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Auxin response factor 2 Alternative name(s): ARF1-binding protein Short name=ARF1-BP Protein MEGAINTEGUMENTA | ||||||||
| Gene names |
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| Organism | Arabidopsis thaliana (Mouse-ear cress) [Complete proteome] | ||||||||
| Taxonomic identifier | 3702 [NCBI] | ||||||||
| Taxonomic lineage | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis |
Protein attributes
| Sequence length | 859 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Auxin response factors (ARFs) are transcriptional factors that binds specifically to the DNA sequence 5'-TGTCTC-3' found in the auxin-responsive promoter elements (AuxREs). Could act as transcriptional activator or repressor. Formation of heterodimers with Aux/IAA proteins may alter their ability to modulate early auxin response genes expression. Promotes flowering, stamen development, floral organ abscission and fruit dehiscence. Functions independently of ethylene and cytokinin response pathways. May act as a repressor of cell division and organ growth. Ref.8 |
| Subunit structure | Homo and heterodimers. Interacts with ARF1. Ref.7 |
| Subcellular location | |
| Tissue specificity | Expressed in the whole plant. Ref.7 |
| Developmental stage | Expressed in the sepals and carpels of young flower buds. At stage 10 of flower development, expression in the carpels becomes restricted to the style. Also expressed in anthers and filaments. At stage 13, expressed in the region at the top of the pedicel, including the abscission zone. Expressed in developing siliques. |
| Domain | Interactions between auxin response factors (ARFs) and Aux/IAA proteins occur through their C-terminal dimerization domains III and IV. |
| Disruption phenotype | Large, dark green rosette leaves, delayed flowering, thick and long inflorescence, abnormal flower morphology and sterility in early formed flowers, but fertility in late-formed flowers. Delayed senescence and abscission. Increased seed size and weight, and extra cell division and expansion in many organs. |
| Sequence similarities | Belongs to the ARF family. Contains 1 Aux/IAA-ARF domain. Contains 1 TF-B3 DNA-binding domain. |
| Caution | Was originally (Ref.4) erroneously termed IAA26 and IAA30. |
| Sequence caution | The sequence AAK55665.1 differs from that shown. Reason: Frameshift at position 657. The sequence AAN46837.1 differs from that shown. Reason: Frameshift at position 657. |
Ontologies
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 859 | 859 | Auxin response factor 2 | PRO_0000111506 | |||||
Regions | |||||||||
| Domain | 734 – 816 | 83 | Aux/IAA-ARF | ||||||
| DNA binding | 164 – 266 | 103 | TF-B3 | ||||||
Experimental info | |||||||||
| Sequence conflict | 491 – 492 | 2 | RR → KK in AAC49752. Ref.5 | ||||||
| Sequence conflict | 491 – 492 | 2 | RR → KK in CAD29696. Ref.6 | ||||||
| Sequence conflict | 491 – 492 | 2 | RR → KK in CAD30210. Ref.6 | ||||||
| Sequence conflict | 520 | 1 | D → E in AAC49752. Ref.5 | ||||||
| Sequence conflict | 520 | 1 | D → E in CAD29696. Ref.6 | ||||||
| Sequence conflict | 520 | 1 | D → E in CAD30210. Ref.6 | ||||||
| Sequence conflict | 596 | 1 | V → A in BAD93968. Ref.4 | ||||||
| Sequence conflict | 675 | 1 | R → K in AAC49752. Ref.5 | ||||||
| Sequence conflict | 675 | 1 | R → K in CAD29696. Ref.6 | ||||||
| Sequence conflict | 675 | 1 | R → K in CAD30210. Ref.6 | ||||||
| Sequence conflict | 823 | 1 | P → Q in AAC49752. Ref.5 | ||||||
| Sequence conflict | 823 | 1 | P → Q in CAD29696. Ref.6 | ||||||
| Sequence conflict | 823 | 1 | P → Q in CAD30210. Ref.6 | ||||||
| Sequence conflict | 826 | 1 | L → V in BAD93959. Ref.4 | ||||||
| Sequence conflict | 826 | 1 | L → V in BAD93897. Ref.4 | ||||||
| Sequence conflict | 826 | 1 | L → V in BAD93891. Ref.4 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Functional genomic analysis of the AUXIN RESPONSE FACTOR gene family members in Arabidopsis thaliana: unique and overlapping functions of ARF7 and ARF19." Okushima Y., Overvoorde P.J., Arima K., Alonso J.M., Chan A., Chang C., Ecker J.R., Hughes B., Lui A., Nguyen D., Onodera C., Quach H., Smith A., Yu G., Theologis A. Plant Cell 17:444-463(2005) [PubMed: 15659631] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia. |
| [2] | "Structural analysis of Arabidopsis thaliana chromosome 5. VII. Sequence features of the regions of 1,013,767 bp covered by sixteen physically assigned P1 and TAC clones." Nakamura Y., Sato S., Asamizu E., Kaneko T., Kotani H., Miyajima N., Tabata S. DNA Res. 5:297-308(1998) [PubMed: 9872454] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia. |
| [3] | "Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R.Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia. |
| [4] | "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. Shinozaki K.Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia. |
| [5] | "ARF1, a transcription factor that binds to auxin response elements." Ulmasov T., Hagen G., Guilfoyle T.J. Science 276:1865-1868(1997) [PubMed: 9188533] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 394-859. Strain: cv. Columbia. |
| [6] | "Nucleotide sequence of the Arabidopsis IAA30." Sessa G., Carabelli M., Ciarbelli A.R., Ruzza V., Steindler C., Ruberti I. Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE OF 406-859. Strain: cv. Columbia. |
| [7] | "Dimerization and DNA binding of auxin response factors." Ulmasov T., Hagen G., Guilfoyle T.J. Plant J. 19:309-319(1999) [PubMed: 10476078] [Abstract] Cited for: DIMERIZATION, TISSUE SPECIFICITY. |
| [8] | "Auxin-responsive gene expression: genes, promoters and regulatory factors." Hagen G., Guilfoyle T.J. Plant Mol. Biol. 49:373-385(2002) [PubMed: 12036261] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE, FUNCTION. |
| [9] | "AUXIN RESPONSE FACTOR1 and AUXIN RESPONSE FACTOR2 regulate senescence and floral organ abscission in Arabidopsis thaliana." Ellis C.M., Nagpal P., Young J.C., Hagen G., Guilfoyle T.J., Reed J.W. Development 132:4563-4574(2005) [PubMed: 16176952] [Abstract] Cited for: FUNCTION, DEVELOPMENTAL STAGE. |
| [10] | "AUXIN RESPONSE FACTOR 2 (ARF2): a pleiotropic developmental regulator." Okushima Y., Mitina I., Quach H.L., Theologis A. Plant J. 43:29-46(2005) [PubMed: 15960614] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE. |
| [11] | "The AUXIN RESPONSE FACTOR 2 gene of Arabidopsis links auxin signalling, cell division, and the size of seeds and other organs." Schruff M.C., Spielman M., Tiwari S., Adams S., Fenby N., Scott R.J. Development 133:251-261(2006) [PubMed: 16339187] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE. |
Cross-references
Sequence databases | |
|---|---|
| AY669787 mRNA. Translation: AAT67071.1. AB016880 Genomic DNA. Translation: BAB10162.1. AF336918 mRNA. Translation: AAG53999.1. AF378862 mRNA. Translation: AAK55665.1. Frameshift. BT000784 mRNA. Translation: AAN31923.1. BT001072 mRNA. Translation: AAN46837.1. Frameshift. AK221252 mRNA. Translation: BAD93891.1. AK221254 mRNA. Translation: BAD93897.1. AK221274 mRNA. Translation: BAD93959.1. AK221277 mRNA. Translation: BAD93968.1. AK221282 mRNA. Translation: BAD93985.1. AK221305 mRNA. Translation: BAD94058.1. U89771 mRNA. Translation: AAC49752.1. Different initiation. AJ441313 mRNA. Translation: CAD29696.1. Different initiation. AJ458328 mRNA. Translation: CAD30210.1. Different initiation. | |
| IPI | IPI00518739. |
| RefSeq | NP_201006.2. NP_851244.1. NP_974980.1. |
| UniGene | At.21704 |
3D structure databases | |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | Q94JM3. 2 interactions. |
Proteomic databases | |
| PRIDE | Q94JM3. |
Genome annotation databases | |
| GeneID | 836321. |
| GenomeReviews | Gene locus AT5G62000 in contig BA000015_GR. |
| KEGG | ath:AT5G62000. |
| NMPDR | fig|3702.1.peg.28232. |
Organism-specific databases | |
| GeneFarm | 3364. 345. |
| TAIR | At5g62000. |
Phylogenomic databases | |
| OMA | Q94JM3. ATAWHAI. |
Gene expression databases | |
| ArrayExpress | Q94JM3. |
| GermOnline | AT5G62000. Arabidopsis thaliana. |
Family and domain databases | |
| InterPro | IPR003311. AUX_IAA. IPR011525. AuxIAA_ARF_dimer. IPR010525. Auxin_resp. IPR003340. TF_B3. [Graphical view] |
| Pfam | PF02309. AUX_IAA. 1 hit. PF06507. Auxin_resp. 1 hit. PF02362. B3. 1 hit. [Graphical view] |
| PROSITE | PS50863. B3. 1 hit. PS50962. IAA_ARF. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | ARFB_ARATH | ||||||||
| Accession | Primary (citable) accession number: Q94JM3 Secondary accession number(s): O23671 Q9FIT6 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | PPAP (Plant Proteome Annotation Project) | ||||||||
Relevant documents
| Arabidopsis thaliana Arabidopsis thaliana: entries and gene names |
| SIMILARITY comments Index of protein domains and families |

Clusters with


