Reviewed, UniProtKB/Swiss-Prot Q94IW5 (C90D2_ORYSJ)

Names and origin

Protein names

Recommended name:
    Cytochrome P450 90D2
    EC=1.14.-.-
Alternative name(s):
    C6-oxidase

Gene names

Name:	CYP90D2
Synonyms:	D2
Ordered Locus Names:	Os01g0197100, LOC_Os01g10040
ORF Names:	P0419B01.11

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	490 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May convert 6-deoxoteasterone to 3-dehydro-6-deoxoteasterone, and teasterone to 3-dehydroteasterone. Catalyzes the C6-oxidation step in brassinosteroids biosynthesis. Involved in the elongation of leaf sheaths and stems. Ref.2
Cofactor	Heme group By similarity.
Subcellular location	Membrane; Single-pass membrane protein Potential.
Tissue specificity	Expressed at low levels leaf blades, shoot apex and elongating stem. Ref.2
Induction	Down-regulated by brassinolide in ebisu dwarf (d2) mutant. Ref.2
Disruption phenotype	Plants show abnormalities in growth: inhibition of second internode elongation, shortened leaf sheaths, erect leaves, and deficiency in skotomorphogenesis. Treatment with exogenous brassinolide rescues the abnormal phenotype. Ref.2
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Membrane
Domain	Transmembrane
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro monooxygenase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 490	490	Cytochrome P450 90D2		PRO_0000052187
Regions
	Transmembrane	4 – 24	21	Potential
Sites
	Metal binding	437	1	Iron (heme axial ligand) By similarity
Experimental info
	Mutagenesis	305	1	P → S in d2-2; dwarf and defective in brassinosteroid biosynthesis. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q94IW5-1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 9EC2853BBAFBB88F	FASTA	490	54,825
	10 20 30 40 50 60 MVSAAAGWAA PAFAVAAVVI WVVLCSELLR RRRRGAGSGK GDAAAAARLP PGSFGWPVVG 70 80 90 100 110 120 ETLEFVSCAY SPRPEAFVDK RRKLHGSAVF RSHLFGSATV VTADAEVSRF VLQSDARAFV 130 140 150 160 170 180 PWYPRSLTEL MGKSSILLIN GALQRRVHGL VGAFFKSSHL KSQLTADMRR RLSPALSSFP 190 200 210 220 230 240 DSSLLHVQHL AKSVVFEILV RGLIGLEAGE EMQQLKQQFQ EFIVGLMSLP IKLPGTRLYR 250 260 270 280 290 300 SLQAKKKMAR LIQRIIREKR ARRAAASPPR DAIDVLIGDG SDELTDELIS DNMIDLMIPA 310 320 330 340 350 360 EDSVPVLITL AVKFLSECPL ALHQLEEENI QLKRRKTDMG ETLQWTDYMS LSFTQHVITE 370 380 390 400 410 420 TLRLGNIIGG IMRKAVRDVE VKGHLIPKGW CVFVYFRSVH LDDTLYDEPY KFNPWRWKEK 430 440 450 460 470 480 DMSNGSFTPF GGGQRLCPGL DLARLEASIF LHHLVTSFRW VAEEDHIVNF PTVRLKRGMP 490 IRVTAKEDDD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence and structure of rice chromosome 1." Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M. , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T. Nature 420:312-316(2002) [PubMed: 12447438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"A rice brassinosteroid-deficient mutant, ebisu dwarf (d2), is caused by a loss of function of a new member of cytochrome P450." Hong Z., Ueguchi-Tanaka M., Umemura K., Uozu S., Fujioka S., Takatsuto S., Yoshida S., Ashikari M., Kitano H., Matsuoka M. Plant Cell 15:2900-2910(2003) [PubMed: 14615594] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, INDUCTION, MUTAGENESIS OF PRO-305, DISRUPTION PHENOTYPE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP003244 Genomic DNA. Translation: BAB56089.1.
RefSeq	NP_001042297.1.
UniGene	Os.43600
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4327329.
KEGG	osa:4327329.
NMPDR	fig|39947.1.peg.5207.
Organism-specific databases
Gramene	Q94IW5.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

C90D2_ORYSJ

Accession

Primary (citable) accession number: Q94IW5

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	December 1, 2001
Last modified:	October 13, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)