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Protein

Protoporphyrinogen oxidase, chloroplastic/mitochondrial

Gene

POX2

Organism
Spinacia oleracea (Spinach)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the 6-electron oxidation of protoporphyrinogen-IX to form protoporphyrin-IX.1 Publication
Provides precursor for the mitochondrial and plastidic heme synthesis and the predominant chlorophyll synthesis in plastids.1 Publication

Catalytic activityi

Protoporphyrinogen-IX + 3 O2 = protoporphyrin-IX + 3 H2O2.

Cofactori

FADBy similarityNote: Binds 1 FAD per subunit.By similarity

Pathwayi: protoporphyrin-IX biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes protoporphyrin-IX from protoporphyrinogen-IX.
Proteins known to be involved in this subpathway in this organism are:
  1. Protoporphyrinogen oxidase, chloroplastic/mitochondrial (POX2)
This subpathway is part of the pathway protoporphyrin-IX biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes protoporphyrin-IX from protoporphyrinogen-IX, the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.

Pathwayi: chlorophyll biosynthesis

This protein is involved in the pathway chlorophyll biosynthesis, which is part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the pathway chlorophyll biosynthesis and in Porphyrin-containing compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei294FAD; via amide nitrogen and carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi49 – 54FADBy similarity6
Nucleotide bindingi72 – 73FADBy similarity2
Nucleotide bindingi94 – 97FADBy similarity4
Nucleotide bindingi501 – 503FADBy similarity3

GO - Molecular functioni

  • oxygen-dependent protoporphyrinogen oxidase activity Source: UniProtKB

GO - Biological processi

  • chlorophyll biosynthetic process Source: UniProtKB
  • heme biosynthetic process Source: UniProtKB
  • protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Chlorophyll biosynthesis, Heme biosynthesis, Porphyrin biosynthesis

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

UniPathwayiUPA00251; UER00324.
UPA00668.

Names & Taxonomyi

Protein namesi
Recommended name:
Protoporphyrinogen oxidase, chloroplastic/mitochondrial (EC:1.3.3.4)
Short name:
Protox II
Alternative name(s):
SO-POX2
Gene namesi
Name:POX2
OrganismiSpinacia oleracea (Spinach)
Taxonomic identifieri3562 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeCaryophyllalesChenopodiaceaeChenopodioideaeAnserineaeSpinacia

Subcellular locationi

GO - Cellular componenti

  • chloroplast inner membrane Source: UniProtKB
  • mitochondrial inner membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Membrane, Mitochondrion, Mitochondrion inner membrane, Plastid, Plastid inner membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi27M → I: Directs expression to chloroplasts only, due to lack of alternative translation. 1 Publication1

Chemistry databases

ChEMBLiCHEMBL2366509.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 48ChloroplastSequence analysisAdd BLAST48
Transit peptidei27 – 48MitochondrionSequence analysisAdd BLAST22
ChainiPRO_000001332749 – 531Protoporphyrinogen oxidase, chloroplastic/mitochondrialAdd BLAST483

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ94IG7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the protoporphyrinogen oxidase family.Curated

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di3.50.50.60. 1 hit.
3.90.660.20. 1 hit.
InterProiIPR002937. Amino_oxidase.
IPR023753. FAD/NAD-binding_dom.
IPR027418. PPOX_C.
IPR004572. Protoporphyrinogen_oxidase.
[Graphical view]
PfamiPF01593. Amino_oxidase. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 2 hits.
TIGRFAMsiTIGR00562. proto_IX_ox. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform L (identifier: Q94IG7-1) [UniParc]FASTAAdd to basket
Also known as: Protox IIL

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVILPVSQLS TNLGLSLVSP TKNNPVMGNV SERNQVNQPI SAKRVAVVGA
60 70 80 90 100
GVSGLAAAYK LKSNGLNVTL FEADSRAGGK LKTVVKDGLI WDEGANTMTE
110 120 130 140 150
SDEEVTSLFD DLGIREKLQL PISQNKRYIA RDGLPVLLPS NPVALLKSNI
160 170 180 190 200
LSAKSKLQIM LEPFLWKKHN GAKVSDENAQ ESVAEFFERH FGKEFVDYLI
210 220 230 240 250
DPFVAGTSGG DPQSLSMRHA FPELWNIENR FGSVISGFIQ SKLSSKKEKG
260 270 280 290 300
GEKQSSNKKP RVRGSFSFQG GMQTLVDTIC KEFGEDELKL QSEVLSLSYS
310 320 330 340 350
HNGSLTSENW SVSSMSNSTI QDQPYDAVVV TAPINNVKEL KIMKVENPFS
360 370 380 390 400
LDFIPEVSCL PLSVIITTFK KTNVKRPLEG FGVLVPSNEQ HNGLKTLGTL
410 420 430 440 450
FSSMMFPDRA PSDVYLYTTF VGGSRNRELA KASTDELKQI VSSDLQQLLG
460 470 480 490 500
TEGEPTFVNH FYWSKAFPLY GRNYDSVLRA IEKMERDLPG LFYAGNHKGG
510 520 530
LSVGKSIASG YKAAELAISY LESNKMTEET I
Length:531
Mass (Da):58,320
Last modified:December 1, 2001 - v1
Checksum:i4D4F900EDCB36C12
GO
Isoform S (identifier: Q94IG7-2) [UniParc]FASTAAdd to basket
Also known as: Protox IIS

The sequence of this isoform differs from the canonical sequence as follows:
     1-26: Missing.

Show »
Length:505
Mass (Da):55,616
Checksum:i578D0238FB7B2B78
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0188051 – 26Missing in isoform S. 1 PublicationAdd BLAST26

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB046993 mRNA. Translation: BAB60710.1.

Keywords - Coding sequence diversityi

Alternative initiation

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB046993 mRNA. Translation: BAB60710.1.

3D structure databases

ProteinModelPortaliQ94IG7.
ModBaseiSearch...
MobiDBiSearch...

Chemistry databases

ChEMBLiCHEMBL2366509.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00251; UER00324.
UPA00668.

Family and domain databases

Gene3Di3.50.50.60. 1 hit.
3.90.660.20. 1 hit.
InterProiIPR002937. Amino_oxidase.
IPR023753. FAD/NAD-binding_dom.
IPR027418. PPOX_C.
IPR004572. Protoporphyrinogen_oxidase.
[Graphical view]
PfamiPF01593. Amino_oxidase. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 2 hits.
TIGRFAMsiTIGR00562. proto_IX_ox. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPPOCM_SPIOL
AccessioniPrimary (citable) accession number: Q94IG7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: December 1, 2001
Last modified: October 5, 2016
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.