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Q94IG7

- PPOCM_SPIOL

UniProt

Q94IG7 - PPOCM_SPIOL

Protein

Protoporphyrinogen oxidase, chloroplastic/mitochondrial

Gene

POX2

Organism
Spinacia oleracea (Spinach)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 56 (01 Oct 2014)
      Sequence version 1 (01 Dec 2001)
      Previous versions | rss
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    Functioni

    Catalyzes the 6-electron oxidation of protoporphyrinogen-IX to form protoporphyrin-IX.1 Publication
    Provides precursor for the mitochondrial and plastidic heme synthesis and the predominant chlorophyll synthesis in plastids.1 Publication

    Catalytic activityi

    Protoporphyrinogen-IX + 3 O2 = protoporphyrin-IX + 3 H2O2.

    Cofactori

    Binds 1 FAD per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei294 – 2941FAD; via amide nitrogen and carbonyl oxygenBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi49 – 546FADBy similarity
    Nucleotide bindingi72 – 732FADBy similarity
    Nucleotide bindingi94 – 974FADBy similarity
    Nucleotide bindingi501 – 5033FADBy similarity

    GO - Molecular functioni

    1. oxygen-dependent protoporphyrinogen oxidase activity Source: UniProtKB

    GO - Biological processi

    1. chlorophyll biosynthetic process Source: UniProtKB
    2. heme biosynthetic process Source: UniProtKB
    3. protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Chlorophyll biosynthesis, Heme biosynthesis, Porphyrin biosynthesis

    Keywords - Ligandi

    FAD, Flavoprotein

    Enzyme and pathway databases

    UniPathwayiUPA00251; UER00324.
    UPA00668.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protoporphyrinogen oxidase, chloroplastic/mitochondrial (EC:1.3.3.4)
    Short name:
    Protox II
    Alternative name(s):
    SO-POX2
    Gene namesi
    Name:POX2
    OrganismiSpinacia oleracea (Spinach)
    Taxonomic identifieri3562 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeCaryophyllalesAmaranthaceaeChenopodioideaeAnserineaeSpinacia

    Subcellular locationi

    GO - Cellular componenti

    1. chloroplast inner membrane Source: UniProtKB
    2. mitochondrial inner membrane Source: UniProtKB

    Keywords - Cellular componenti

    Chloroplast, Membrane, Mitochondrion, Mitochondrion inner membrane, Plastid, Plastid inner membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi27 – 271M → I: Directs expression to chloroplasts only, due to lack of alternative translation. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 4848ChloroplastSequence AnalysisAdd
    BLAST
    Transit peptidei27 – 4822MitochondrionSequence AnalysisAdd
    BLAST
    Chaini49 – 531483Protoporphyrinogen oxidase, chloroplastic/mitochondrialPRO_0000013327Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ94IG7.
    SMRiQ94IG7. Positions 42-523.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the protoporphyrinogen oxidase family.Curated

    Keywords - Domaini

    Transit peptide

    Family and domain databases

    Gene3Di3.90.660.20. 1 hit.
    InterProiIPR002937. Amino_oxidase.
    IPR027418. PPOX_C.
    IPR004572. Protoporphyrinogen_oxidase.
    [Graphical view]
    PfamiPF01593. Amino_oxidase. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00562. proto_IX_ox. 1 hit.

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative initiation. Align

    Isoform L (identifier: Q94IG7-1) [UniParc]FASTAAdd to Basket

    Also known as: Protox IIL

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MVILPVSQLS TNLGLSLVSP TKNNPVMGNV SERNQVNQPI SAKRVAVVGA    50
    GVSGLAAAYK LKSNGLNVTL FEADSRAGGK LKTVVKDGLI WDEGANTMTE 100
    SDEEVTSLFD DLGIREKLQL PISQNKRYIA RDGLPVLLPS NPVALLKSNI 150
    LSAKSKLQIM LEPFLWKKHN GAKVSDENAQ ESVAEFFERH FGKEFVDYLI 200
    DPFVAGTSGG DPQSLSMRHA FPELWNIENR FGSVISGFIQ SKLSSKKEKG 250
    GEKQSSNKKP RVRGSFSFQG GMQTLVDTIC KEFGEDELKL QSEVLSLSYS 300
    HNGSLTSENW SVSSMSNSTI QDQPYDAVVV TAPINNVKEL KIMKVENPFS 350
    LDFIPEVSCL PLSVIITTFK KTNVKRPLEG FGVLVPSNEQ HNGLKTLGTL 400
    FSSMMFPDRA PSDVYLYTTF VGGSRNRELA KASTDELKQI VSSDLQQLLG 450
    TEGEPTFVNH FYWSKAFPLY GRNYDSVLRA IEKMERDLPG LFYAGNHKGG 500
    LSVGKSIASG YKAAELAISY LESNKMTEET I 531
    Length:531
    Mass (Da):58,320
    Last modified:December 1, 2001 - v1
    Checksum:i4D4F900EDCB36C12
    GO
    Isoform S (identifier: Q94IG7-2) [UniParc]FASTAAdd to Basket

    Also known as: Protox IIS

    The sequence of this isoform differs from the canonical sequence as follows:
         1-26: Missing.

    Show »
    Length:505
    Mass (Da):55,616
    Checksum:i578D0238FB7B2B78
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 2626Missing in isoform S. 1 PublicationVSP_018805Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB046993 mRNA. Translation: BAB60710.1.

    Keywords - Coding sequence diversityi

    Alternative initiation

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB046993 mRNA. Translation: BAB60710.1 .

    3D structure databases

    ProteinModelPortali Q94IG7.
    SMRi Q94IG7. Positions 42-523.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00251 ; UER00324 .
    UPA00668 .

    Family and domain databases

    Gene3Di 3.90.660.20. 1 hit.
    InterProi IPR002937. Amino_oxidase.
    IPR027418. PPOX_C.
    IPR004572. Protoporphyrinogen_oxidase.
    [Graphical view ]
    Pfami PF01593. Amino_oxidase. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00562. proto_IX_ox. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Dual targeting of spinach protoporphyrinogen oxidase II to mitochondria and chloroplasts by alternative use of two in-frame initiation codons."
      Watanabe N., Che F.-S., Iwano M., Takayama S., Yoshida S., Isogai A.
      J. Biol. Chem. 276:20474-20481(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS L AND S), FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF MET-27.
      Tissue: Leaf.

    Entry informationi

    Entry nameiPPOCM_SPIOL
    AccessioniPrimary (citable) accession number: Q94IG7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 4, 2005
    Last sequence update: December 1, 2001
    Last modified: October 1, 2014
    This is version 56 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3