SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q94IG7

- PPOCM_SPIOL

UniProt

Q94IG7 - PPOCM_SPIOL

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Protoporphyrinogen oxidase, chloroplastic/mitochondrial
Gene
POX2
Organism
Spinacia oleracea (Spinach)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the 6-electron oxidation of protoporphyrinogen-IX to form protoporphyrin-IX.1 Publication
Provides precursor for the mitochondrial and plastidic heme synthesis and the predominant chlorophyll synthesis in plastids.1 Publication

Catalytic activityi

Protoporphyrinogen-IX + 3 O2 = protoporphyrin-IX + 3 H2O2.

Cofactori

Binds 1 FAD per subunit By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei294 – 2941FAD; via amide nitrogen and carbonyl oxygen By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi49 – 546FAD By similarity
Nucleotide bindingi72 – 732FAD By similarity
Nucleotide bindingi94 – 974FAD By similarity
Nucleotide bindingi501 – 5033FAD By similarity

GO - Molecular functioni

  1. oxygen-dependent protoporphyrinogen oxidase activity Source: UniProtKB
Complete GO annotation...

GO - Biological processi

  1. chlorophyll biosynthetic process Source: UniProtKB
  2. heme biosynthetic process Source: UniProtKB
  3. protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Chlorophyll biosynthesis, Heme biosynthesis, Porphyrin biosynthesis

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

UniPathwayiUPA00251; UER00324.
UPA00668.

Names & Taxonomyi

Protein namesi
Recommended name:
Protoporphyrinogen oxidase, chloroplastic/mitochondrial (EC:1.3.3.4)
Short name:
Protox II
Alternative name(s):
SO-POX2
Gene namesi
Name:POX2
OrganismiSpinacia oleracea (Spinach)
Taxonomic identifieri3562 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeCaryophyllalesAmaranthaceaeChenopodioideaeAnserineaeSpinacia

Subcellular locationi

Isoform L : Plastidchloroplast inner membrane; Peripheral membrane protein; Stromal side 1 Publication
Isoform S : Mitochondrion inner membrane; Peripheral membrane protein 1 Publication

GO - Cellular componenti

  1. chloroplast inner membrane Source: UniProtKB
  2. mitochondrial inner membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Membrane, Mitochondrion, Mitochondrion inner membrane, Plastid, Plastid inner membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi27 – 271M → I: Directs expression to chloroplasts only, due to lack of alternative translation. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 4848Chloroplast Reviewed prediction
Add
BLAST
Transit peptidei27 – 4822Mitochondrion Reviewed prediction
Add
BLAST
Chaini49 – 531483Protoporphyrinogen oxidase, chloroplastic/mitochondrial
PRO_0000013327Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ94IG7.
SMRiQ94IG7. Positions 42-523.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di3.90.660.20. 1 hit.
InterProiIPR002937. Amino_oxidase.
IPR027418. PPOX_C.
IPR004572. Protoporphyrinogen_oxidase.
[Graphical view]
PfamiPF01593. Amino_oxidase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00562. proto_IX_ox. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative initiation. Align

Isoform L (identifier: Q94IG7-1) [UniParc]FASTAAdd to Basket

Also known as: Protox IIL

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MVILPVSQLS TNLGLSLVSP TKNNPVMGNV SERNQVNQPI SAKRVAVVGA    50
GVSGLAAAYK LKSNGLNVTL FEADSRAGGK LKTVVKDGLI WDEGANTMTE 100
SDEEVTSLFD DLGIREKLQL PISQNKRYIA RDGLPVLLPS NPVALLKSNI 150
LSAKSKLQIM LEPFLWKKHN GAKVSDENAQ ESVAEFFERH FGKEFVDYLI 200
DPFVAGTSGG DPQSLSMRHA FPELWNIENR FGSVISGFIQ SKLSSKKEKG 250
GEKQSSNKKP RVRGSFSFQG GMQTLVDTIC KEFGEDELKL QSEVLSLSYS 300
HNGSLTSENW SVSSMSNSTI QDQPYDAVVV TAPINNVKEL KIMKVENPFS 350
LDFIPEVSCL PLSVIITTFK KTNVKRPLEG FGVLVPSNEQ HNGLKTLGTL 400
FSSMMFPDRA PSDVYLYTTF VGGSRNRELA KASTDELKQI VSSDLQQLLG 450
TEGEPTFVNH FYWSKAFPLY GRNYDSVLRA IEKMERDLPG LFYAGNHKGG 500
LSVGKSIASG YKAAELAISY LESNKMTEET I 531
Length:531
Mass (Da):58,320
Last modified:December 1, 2001 - v1
Checksum:i4D4F900EDCB36C12
GO
Isoform S (identifier: Q94IG7-2) [UniParc]FASTAAdd to Basket

Also known as: Protox IIS

The sequence of this isoform differs from the canonical sequence as follows:
     1-26: Missing.

Show »
Length:505
Mass (Da):55,616
Checksum:i578D0238FB7B2B78
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 2626Missing in isoform S.
VSP_018805Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB046993 mRNA. Translation: BAB60710.1.

Keywords - Coding sequence diversityi

Alternative initiation

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB046993 mRNA. Translation: BAB60710.1 .

3D structure databases

ProteinModelPortali Q94IG7.
SMRi Q94IG7. Positions 42-523.
ModBasei Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00251 ; UER00324 .
UPA00668 .

Family and domain databases

Gene3Di 3.90.660.20. 1 hit.
InterProi IPR002937. Amino_oxidase.
IPR027418. PPOX_C.
IPR004572. Protoporphyrinogen_oxidase.
[Graphical view ]
Pfami PF01593. Amino_oxidase. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00562. proto_IX_ox. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Dual targeting of spinach protoporphyrinogen oxidase II to mitochondria and chloroplasts by alternative use of two in-frame initiation codons."
    Watanabe N., Che F.-S., Iwano M., Takayama S., Yoshida S., Isogai A.
    J. Biol. Chem. 276:20474-20481(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS L AND S), FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF MET-27.
    Tissue: Leaf.

Entry informationi

Entry nameiPPOCM_SPIOL
AccessioniPrimary (citable) accession number: Q94IG7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: December 1, 2001
Last modified: September 3, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi