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Q94IG7 (PPOCM_SPIOL) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 54. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protoporphyrinogen oxidase, chloroplastic/mitochondrial

Short name=Protox II
EC=1.3.3.4
Alternative name(s):
SO-POX2
Gene names
Name:POX2
OrganismSpinacia oleracea (Spinach)
Taxonomic identifier3562 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeCaryophyllalesAmaranthaceaeChenopodioideaeAnserineaeSpinacia

Protein attributes

Sequence length531 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the 6-electron oxidation of protoporphyrinogen-IX to form protoporphyrin-IX. Ref.1

Provides precursor for the mitochondrial and plastidic heme synthesis and the predominant chlorophyll synthesis in plastids. Ref.1

Catalytic activity

Protoporphyrinogen-IX + 3 O2 = protoporphyrin-IX + 3 H2O2.

Cofactor

Binds 1 FAD per subunit By similarity.

Pathway

Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; protoporphyrin-IX from protoporphyrinogen-IX: step 1/1.

Porphyrin-containing compound metabolism; chlorophyll biosynthesis.

Subunit structure

Homodimer By similarity.

Subcellular location

Isoform L: Plastidchloroplast inner membrane; Peripheral membrane protein; Stromal side Ref.1.

Isoform S: Mitochondrion inner membrane; Peripheral membrane protein Ref.1.

Sequence similarities

Belongs to the protoporphyrinogen oxidase family.

Alternative products

This entry describes 2 isoforms produced by alternative initiation. [Align] [Select]
Isoform L (identifier: Q94IG7-1)

Also known as: Protox IIL;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform S (identifier: Q94IG7-2)

Also known as: Protox IIS;

The sequence of this isoform differs from the canonical sequence as follows:
     1-26: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 4848Chloroplast Potential
Transit peptide27 – 4822Mitochondrion Potential
Chain49 – 531483Protoporphyrinogen oxidase, chloroplastic/mitochondrial
PRO_0000013327

Regions

Nucleotide binding49 – 546FAD By similarity
Nucleotide binding72 – 732FAD By similarity
Nucleotide binding94 – 974FAD By similarity
Nucleotide binding501 – 5033FAD By similarity

Sites

Binding site2941FAD; via amide nitrogen and carbonyl oxygen By similarity

Natural variations

Alternative sequence1 – 2626Missing in isoform S.
VSP_018805

Experimental info

Mutagenesis271M → I: Directs expression to chloroplasts only, due to lack of alternative translation. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform L (Protox IIL) [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: 4D4F900EDCB36C12

FASTA53158,320
        10         20         30         40         50         60 
MVILPVSQLS TNLGLSLVSP TKNNPVMGNV SERNQVNQPI SAKRVAVVGA GVSGLAAAYK 

        70         80         90        100        110        120 
LKSNGLNVTL FEADSRAGGK LKTVVKDGLI WDEGANTMTE SDEEVTSLFD DLGIREKLQL 

       130        140        150        160        170        180 
PISQNKRYIA RDGLPVLLPS NPVALLKSNI LSAKSKLQIM LEPFLWKKHN GAKVSDENAQ 

       190        200        210        220        230        240 
ESVAEFFERH FGKEFVDYLI DPFVAGTSGG DPQSLSMRHA FPELWNIENR FGSVISGFIQ 

       250        260        270        280        290        300 
SKLSSKKEKG GEKQSSNKKP RVRGSFSFQG GMQTLVDTIC KEFGEDELKL QSEVLSLSYS 

       310        320        330        340        350        360 
HNGSLTSENW SVSSMSNSTI QDQPYDAVVV TAPINNVKEL KIMKVENPFS LDFIPEVSCL 

       370        380        390        400        410        420 
PLSVIITTFK KTNVKRPLEG FGVLVPSNEQ HNGLKTLGTL FSSMMFPDRA PSDVYLYTTF 

       430        440        450        460        470        480 
VGGSRNRELA KASTDELKQI VSSDLQQLLG TEGEPTFVNH FYWSKAFPLY GRNYDSVLRA 

       490        500        510        520        530 
IEKMERDLPG LFYAGNHKGG LSVGKSIASG YKAAELAISY LESNKMTEET I 

« Hide

Isoform S (Protox IIS) [UniParc].

Checksum: 578D0238FB7B2B78
Show »

FASTA50555,616

References

[1]"Dual targeting of spinach protoporphyrinogen oxidase II to mitochondria and chloroplasts by alternative use of two in-frame initiation codons."
Watanabe N., Che F.-S., Iwano M., Takayama S., Yoshida S., Isogai A.
J. Biol. Chem. 276:20474-20481(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS L AND S), FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF MET-27.
Tissue: Leaf.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB046993 mRNA. Translation: BAB60710.1.

3D structure databases

ProteinModelPortalQ94IG7.
SMRQ94IG7. Positions 42-523.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00251; UER00324.
UPA00668.

Family and domain databases

Gene3D3.90.660.20. 1 hit.
InterProIPR002937. Amino_oxidase.
IPR004572. Protoporphyrinogen_oxidase.
IPR027418. Protoporphyrinogen_oxidase_C.
[Graphical view]
PfamPF01593. Amino_oxidase. 1 hit.
[Graphical view]
TIGRFAMsTIGR00562. proto_IX_ox. 1 hit.
ProtoNetSearch...

Entry information

Entry namePPOCM_SPIOL
AccessionPrimary (citable) accession number: Q94IG7
Entry history
Integrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: December 1, 2001
Last modified: February 19, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways