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Protein

Protoporphyrinogen oxidase 2

Gene

POX2

Organism
Spinacia oleracea (Spinach)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the 6-electron oxidation of protoporphyrinogen-IX to form protoporphyrin-IX.1 Publication
Provides precursor for the mitochondrial and plastidic heme synthesis and the predominant chlorophyll synthesis in plastids.1 Publication

Catalytic activityi

Protoporphyrinogen-IX + 3 O2 = protoporphyrin-IX + 3 H2O2.1 Publication

Cofactori

FADBy similarityNote: Binds 1 FAD per subunit.By similarity

Pathwayi: protoporphyrin-IX biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes protoporphyrin-IX from protoporphyrinogen-IX.Curated
Proteins known to be involved in this subpathway in this organism are:
  1. Protoporphyrinogen oxidase 1, chloroplastic (POX1), Protoporphyrinogen oxidase 2 (POX2), Protoporphyrinogen oxidase (SOVF_165410), Protoporphyrinogen oxidase (SOVF_102200), Protoporphyrinogen oxidase (SOVF_216440)
This subpathway is part of the pathway protoporphyrin-IX biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes protoporphyrin-IX from protoporphyrinogen-IX, the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.

Pathwayi: chlorophyll biosynthesis

This protein is involved in the pathway chlorophyll biosynthesis, which is part of Porphyrin-containing compound metabolism.Curated
View all proteins of this organism that are known to be involved in the pathway chlorophyll biosynthesis and in Porphyrin-containing compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei294FAD; via amide nitrogen and carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi49 – 54FADBy similarity6
Nucleotide bindingi72 – 73FADBy similarity2
Nucleotide bindingi94 – 97FADBy similarity4
Nucleotide bindingi501 – 503FADBy similarity3

GO - Molecular functioni

  • oxygen-dependent protoporphyrinogen oxidase activity Source: UniProtKB

GO - Biological processi

  • chlorophyll biosynthetic process Source: UniProtKB
  • heme biosynthetic process Source: UniProtKB
  • protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway

Keywordsi

Molecular functionOxidoreductase
Biological processChlorophyll biosynthesis, Heme biosynthesis, Porphyrin biosynthesis
LigandFAD, Flavoprotein

Enzyme and pathway databases

UniPathwayiUPA00251; UER00324
UPA00668

Names & Taxonomyi

Protein namesi
Recommended name:
Protoporphyrinogen oxidase 2Curated (EC:1.3.3.41 Publication)
Short name:
Protox II1 Publication
Alternative name(s):
SO-POX21 Publication
Gene namesi
Name:POX21 Publication
OrganismiSpinacia oleracea (Spinach)
Taxonomic identifieri3562 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeCaryophyllalesChenopodiaceaeChenopodioideaeAnserineaeSpinacia

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Membrane, Mitochondrion, Mitochondrion inner membrane, Plastid, Plastid inner membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi27M → I: Targeted to chloroplasts only, due to lack of alternative initiation. 1 Publication1

Chemistry databases

ChEMBLiCHEMBL2366509

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 48ChloroplastSequence analysisAdd BLAST48
ChainiPRO_000001332749 – 531Protoporphyrinogen oxidase 2Add BLAST483

Proteomic databases

PRIDEiQ94IG7

Structurei

3D structure databases

ProteinModelPortaliQ94IG7
SMRiQ94IG7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the protoporphyrinogen oxidase family.Curated

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di3.50.50.60, 3 hits
InterProiView protein in InterPro
IPR002937 Amino_oxidase
IPR036188 FAD/NAD-bd_sf
IPR004572 Protoporphyrinogen_oxidase
PfamiView protein in Pfam
PF01593 Amino_oxidase, 1 hit
SUPFAMiSSF51905 SSF51905, 2 hits
TIGRFAMsiTIGR00562 proto_IX_ox, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform L (identifier: Q94IG7-1) [UniParc]FASTAAdd to basket
Also known as: Protox IIL

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVILPVSQLS TNLGLSLVSP TKNNPVMGNV SERNQVNQPI SAKRVAVVGA
60 70 80 90 100
GVSGLAAAYK LKSNGLNVTL FEADSRAGGK LKTVVKDGLI WDEGANTMTE
110 120 130 140 150
SDEEVTSLFD DLGIREKLQL PISQNKRYIA RDGLPVLLPS NPVALLKSNI
160 170 180 190 200
LSAKSKLQIM LEPFLWKKHN GAKVSDENAQ ESVAEFFERH FGKEFVDYLI
210 220 230 240 250
DPFVAGTSGG DPQSLSMRHA FPELWNIENR FGSVISGFIQ SKLSSKKEKG
260 270 280 290 300
GEKQSSNKKP RVRGSFSFQG GMQTLVDTIC KEFGEDELKL QSEVLSLSYS
310 320 330 340 350
HNGSLTSENW SVSSMSNSTI QDQPYDAVVV TAPINNVKEL KIMKVENPFS
360 370 380 390 400
LDFIPEVSCL PLSVIITTFK KTNVKRPLEG FGVLVPSNEQ HNGLKTLGTL
410 420 430 440 450
FSSMMFPDRA PSDVYLYTTF VGGSRNRELA KASTDELKQI VSSDLQQLLG
460 470 480 490 500
TEGEPTFVNH FYWSKAFPLY GRNYDSVLRA IEKMERDLPG LFYAGNHKGG
510 520 530
LSVGKSIASG YKAAELAISY LESNKMTEET I
Length:531
Mass (Da):58,320
Last modified:December 1, 2001 - v1
Checksum:i4D4F900EDCB36C12
GO
Isoform S (identifier: Q94IG7-2) [UniParc]FASTAAdd to basket
Also known as: Protox IIS

The sequence of this isoform differs from the canonical sequence as follows:
     1-26: Missing.

Note: Produced by alternative initiation at Met-27 of isoform S. Mitochondrial precursor. Contains a mitochondrial transit peptide from 1 to 22 which is removed in mature form.1 Publication
Show »
Length:505
Mass (Da):55,616
Checksum:i578D0238FB7B2B78
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0188051 – 26Missing in isoform S. 1 PublicationAdd BLAST26

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB046993 mRNA Translation: BAB60710.1

Keywords - Coding sequence diversityi

Alternative initiation

Similar proteinsi

Entry informationi

Entry nameiPPOCM_SPIOL
AccessioniPrimary (citable) accession number: Q94IG7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: December 1, 2001
Last modified: May 23, 2018
This is version 69 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

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