Q94IG7 (PPOCM_SPIOL) Reviewed, UniProtKB/Swiss-Prot
Last modified
May 29, 2013.
Version 53.
History...
Clusters with 
Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Protoporphyrinogen oxidase, chloroplastic/mitochondrial Short name=Protox II EC=1.3.3.4 Alternative name(s): SO-POX2 | ||
| Gene names |
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| Organism | Spinacia oleracea (Spinach) | ||
| Taxonomic identifier | 3562 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › Caryophyllales › Amaranthaceae › Chenopodioideae › Anserineae › Spinacia |
Protein attributes
| Sequence length | 531 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Catalyzes the 6-electron oxidation of protoporphyrinogen-IX to form protoporphyrin-IX. Ref.1 Provides precursor for the mitochondrial and plastidic heme synthesis and the predominant chlorophyll synthesis in plastids. Ref.1 |
| Catalytic activity | Protoporphyrinogen-IX + 3 O2 = protoporphyrin-IX + 3 H2O2. |
| Cofactor | FAD By similarity. |
| Pathway | Porphyrin-containing compound metabolism; chlorophyll biosynthesis. |
| Subunit structure | Homodimer; contains one FAD per homodimer By similarity. |
| Subcellular location | Isoform L: Plastid › chloroplast inner membrane; Peripheral membrane protein; Stromal side Ref.1. Isoform S: Mitochondrion inner membrane; Peripheral membrane protein Ref.1. |
| Sequence similarities | Belongs to the protoporphyrinogen oxidase family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Chlorophyll biosynthesis Heme biosynthesis Porphyrin biosynthesis |
| Cellular component | Chloroplast Membrane Mitochondrion Mitochondrion inner membrane Plastid Plastid inner membrane |
| Coding sequence diversity | Alternative initiation |
| Domain | Transit peptide |
| Ligand | FAD Flavoprotein |
| Molecular function | Oxidoreductase |
| Gene Ontology (GO) | |
| Biological_process | chlorophyll biosynthetic process Traceable author statement Ref.1. Source: UniProtKB heme biosynthetic processTraceable author statement Ref.1. Source: UniProtKB protoporphyrinogen IX biosynthetic processInferred from electronic annotation. Source: UniProtKB-UniPathway |
| Cellular_component | chloroplast inner membrane Inferred from direct assay Ref.1. Source: UniProtKB mitochondrial inner membraneInferred from direct assay Ref.1. Source: UniProtKB |
| Molecular_function | oxygen-dependent protoporphyrinogen oxidase activity Inferred from direct assay Ref.1. Source: UniProtKB |
| Complete GO annotation... | |
Alternative products
| This entry describes 2 isoforms produced by alternative initiation. [Align] [Select] | ||||||
| Isoform L (identifier: Q94IG7-1) Also known as: Protox IIL; This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform S (identifier: Q94IG7-2) Also known as: Protox IIS; The sequence of this isoform differs from the canonical sequence as follows: 1-26: Missing. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Transit peptide | 1 – 48 | 48 | Chloroplast Potential | ||||||
| Transit peptide | 27 – 48 | 22 | Mitochondrion Potential | ||||||
| Chain | 49 – 531 | 483 | Protoporphyrinogen oxidase, chloroplastic/mitochondrial | PRO_0000013327 | |||||
Regions | |||||||||
| Nucleotide binding | 49 – 54 | 6 | FAD Potential | ||||||
Natural variations | |||||||||
| Alternative sequence | 1 – 26 | 26 | Missing in isoform S. | VSP_018805 | |||||
Experimental info | |||||||||
| Mutagenesis | 27 | 1 | M → I: Directs expression to chloroplasts only, due to lack of alternative translation. Ref.1 | ||||||
Sequences
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References
| [1] | "Dual targeting of spinach protoporphyrinogen oxidase II to mitochondria and chloroplasts by alternative use of two in-frame initiation codons." Watanabe N., Che F.-S., Iwano M., Takayama S., Yoshida S., Isogai A. J. Biol. Chem. 276:20474-20481(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS L AND S), FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF MET-27. Tissue: Leaf. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AB046993 mRNA. Translation: BAB60710.1. |
3D structure databases | |
| ProteinModelPortal | Q94IG7. |
| SMR | Q94IG7. Positions 42-523. |
| ModBase | Search... |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Enzyme and pathway databases | |
| UniPathway | UPA00251; UER00324. UPA00668. |
Family and domain databases | |
| Gene3D | 3.90.660.20. 1 hit. |
| InterPro | IPR002937. Amino_oxidase. IPR004572. Protoporphyrinogen_oxidase. IPR027418. Protoporphyrinogen_oxidase_C. [Graphical view] |
| Pfam | PF01593. Amino_oxidase. 1 hit. [Graphical view] |
| TIGRFAMs | TIGR00562. proto_IX_ox. 1 hit. |
| ProtoNet | Search... |
Entry information
| Entry name | PPOCM_SPIOL | ||||||||
| Accession | Primary (citable) accession number: Q94IG7 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Plant Protein Annotation Program | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |