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Protein

3-epi-6-deoxocathasterone 23-monooxygenase

Gene

CYP90D1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

C-23 hydroxylase involved in brassinosteroids biosynthesis. Converts directly (22S,24R)-22-hydroxy-5-alpha-ergostan-3-one and 3-epi-6-deoxocathasterone to 3-dehydro-6-deoxoteasterone and 6-deoxotyphasterol. These C-23 hydroxylation shortcuts bypass campestanol, 6-deoxocathasterone, and 6-deoxoteasterone. Functionally redundant with CYP90C1.2 Publications

Catalytic activityi

3-epi-6-deoxocathasterone + NADPH + O2 = 6-deoxotyphasterol + NADP+ + H2O.
(22S,24R)-22-hydroxy-5-alpha-ergostan-3-one + NADPH + O2 = 3-dehydro-6-deoxoteasterone + NADP+ + H2O.

Cofactori

hemeBy similarity

Pathwayi: brassinosteroid biosynthesis

This protein is involved in the pathway brassinosteroid biosynthesis, which is part of Plant hormone biosynthesis.
View all proteins of this organism that are known to be involved in the pathway brassinosteroid biosynthesis and in Plant hormone biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi442Iron (heme axial ligand)By similarity1

GO - Molecular functioni

GO - Biological processi

  • brassinosteroid biosynthetic process Source: TAIR
  • brassinosteroid homeostasis Source: GO_Central
  • leaf development Source: TAIR
  • petal development Source: TAIR
  • stamen development Source: TAIR
  • sterol metabolic process Source: GO_Central

Keywordsi

Molecular functionMonooxygenase, Oxidoreductase
Biological processBrassinosteroid biosynthesis, Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis
LigandHeme, Iron, Metal-binding, NADP

Enzyme and pathway databases

BioCyciARA:AT3G13730-MONOMER.
MetaCyc:AT3G13730-MONOMER.
ReactomeiR-ATH-211916. Vitamins.
R-ATH-5365859. RA biosynthesis pathway.
UniPathwayiUPA00381.

Names & Taxonomyi

Protein namesi
Recommended name:
3-epi-6-deoxocathasterone 23-monooxygenase (EC:1.14.13.112)
Alternative name(s):
Cytochrome P450 90D1
Gene namesi
Name:CYP90D1
Ordered Locus Names:At3g13730
ORF Names:MMM17.20
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G13730.
TAIRilocus:2091571. AT3G13730.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei7 – 27HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003949991 – 4913-epi-6-deoxocathasterone 23-monooxygenaseAdd BLAST491

Proteomic databases

PaxDbiQ94IA6.

Expressioni

Tissue specificityi

Expressed in leaf vascular tissue.1 Publication

Gene expression databases

ExpressionAtlasiQ94IA6. baseline and differential.
GenevisibleiQ94IA6. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT3G13730.1.

Structurei

3D structure databases

ProteinModelPortaliQ94IA6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0157. Eukaryota.
COG2124. LUCA.
HOGENOMiHOG000237614.
InParanoidiQ94IA6.
KOiK12638.
OMAiSAFVPWY.
OrthoDBiEOG09360A4G.
PhylomeDBiQ94IA6.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiView protein in InterPro
IPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR036396. Cyt_P450_sf.
PfamiView protein in Pfam
PF00067. p450. 1 hit.
PRINTSiPR00463. EP450I.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiView protein in PROSITE
PS00086. CYTOCHROME_P450. 1 hit.

Sequencei

Sequence statusi: Complete.

Q94IA6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDTSSSLLFF SFFFFIIIVI FNKINGLRSS PASKKKLNDH HVTSQSHGPK
60 70 80 90 100
FPHGSLGWPV IGETIEFVSS AYSDRPESFM DKRRLMYGRV FKSHIFGTAT
110 120 130 140 150
IVSTDAEVNR AVLQSDSTAF VPFYPKTVRE LMGKSSILLI NGSLHRRFHG
160 170 180 190 200
LVGSFLKSPL LKAQIVRDMH KFLSESMDLW SEDQPVLLQD VSKTVAFKVL
210 220 230 240 250
AKALISVEKG EDLEELKREF ENFISGLMSL PINFPGTQLH RSLQAKKNMV
260 270 280 290 300
KQVERIIEGK IRKTKNKEED DVIAKDVVDV LLKDSSEHLT HNLIANNMID
310 320 330 340 350
MMIPGHDSVP VLITLAVKFL SDSPAALNLL TEENMKLKSL KELTGEPLYW
360 370 380 390 400
NDYLSLPFTQ KVITETLRMG NVIIGVMRKA MKDVEIKGYV IPKGWCFLAY
410 420 430 440 450
LRSVHLDKLY YESPYKFNPW RWQERDMNTS SFSPFGGGQR LCPGLDLARL
460 470 480 490
ETSVFLHHLV TRFRWIAEED TIINFPTVHM KNKLPIWIKR I
Length:491
Mass (Da):56,153
Last modified:December 1, 2001 - v1
Checksum:i02FB908A91995A40
GO

Sequence cautioni

The sequence BAB01922 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB066286 mRNA. Translation: BAB62109.1.
AP001307 Genomic DNA. Translation: BAB01922.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE75404.1.
BT004084 mRNA. Translation: AAO42111.1.
BT005093 mRNA. Translation: AAO50626.1.
RefSeqiNP_001327080.1. NM_001338068.1.
NP_566462.1. NM_112223.3.
UniGeneiAt.24699.

Genome annotation databases

EnsemblPlantsiAT3G13730.1; AT3G13730.1; AT3G13730.
GeneIDi820582.
GrameneiAT3G13730.1; AT3G13730.1; AT3G13730.
KEGGiath:AT3G13730.

Similar proteinsi

Entry informationi

Entry nameiC90D1_ARATH
AccessioniPrimary (citable) accession number: Q94IA6
Secondary accession number(s): Q9LIC5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 15, 2010
Last sequence update: December 1, 2001
Last modified: October 25, 2017
This is version 113 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families