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Protein

Sec-independent protein translocase protein TATC, chloroplastic

Gene

TATC

Organism
Pisum sativum (Garden pea)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Part of the twin-arginine translocation (Tat) system that transports large folded proteins containing a characteristic twin-arginine motif in their signal peptide across the thylakoid membrane. Involved in delta pH-dependent protein transport required for chloroplast development, especially thylakoid membrane formation. TATC and TATB mediate precursor recognition, whereas TATA facilitates translocation.4 Publications

GO - Biological processi

  • protein import into chloroplast thylakoid membrane Source: UniProtKB
  • protein transport by the Tat complex Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Protein transport, Translocation, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Sec-independent protein translocase protein TATC, chloroplastic
Alternative name(s):
Protein TWIN-ARGININE TRANSLOCATION C
Short name:
cpTatC
Gene namesi
Name:TATC
OrganismiPisum sativum (Garden pea)
Taxonomic identifieri3888 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeFabeaePisum

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini51 – 13686StromalSequence analysisAdd
BLAST
Transmembranei137 – 15721HelicalSequence analysisAdd
BLAST
Topological domaini158 – 19235LumenalSequence analysisAdd
BLAST
Transmembranei193 – 21321HelicalSequence analysisAdd
BLAST
Topological domaini214 – 22310StromalSequence analysis
Transmembranei224 – 24421HelicalSequence analysisAdd
BLAST
Topological domaini245 – 27026LumenalSequence analysisAdd
BLAST
Transmembranei271 – 29121HelicalSequence analysisAdd
BLAST
Topological domaini292 – 30211StromalSequence analysisAdd
BLAST
Transmembranei303 – 32321HelicalSequence analysisAdd
BLAST
Topological domaini324 – 3307LumenalSequence analysis
Transmembranei331 – 35121HelicalSequence analysisAdd
BLAST
Topological domaini352 – 3532StromalSequence analysis

GO - Cellular componenti

  • chloroplast thylakoid membrane Source: UniProtKB
  • integral component of membrane Source: UniProtKB-KW
  • TAT protein transport complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Membrane, Plastid, Thylakoid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 353Sec-independent protein translocase protein TATC, chloroplasticPRO_0000419911
Transit peptidei1 – 5050ChloroplastSequence analysisAdd
BLAST
Transit peptidei51 – ?ThylakoidSequence analysis

Interactioni

Subunit structurei

In thylakoid membranes, TATC and TATB form a large receptor complex, containing about eight TATC-TATB pairs, which binds the precursor protein. Twin arginine signal peptide promotes pH-triggered docking of TATA oligomers to TATC-TATB receptor complex, inducing a conformational switch of TATA that results in activation of the translocase. TATA dissociates from TATC-TATB upon completion of translocation. According to PubMed:22564412, it is estimated that the translocase fully saturated with precursor proteins and TATA is an 2.2-megadalton complex that can individually transport eight precursor proteins or cooperatively transport multimeric precursors.5 Publications

Family & Domainsi

Sequence similaritiesi

Belongs to the TatC family.Curated

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Family and domain databases

HAMAPiMF_00902. TatC.
InterProiIPR019820. Sec-indep_translocase_CS.
IPR002033. TatC.
[Graphical view]
PfamiPF00902. TatC. 1 hit.
[Graphical view]
PRINTSiPR01840. TATCFAMILY.
TIGRFAMsiTIGR00945. tatC. 1 hit.
PROSITEiPS01218. TATC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q94G17-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLGTTTVPT NILPQFGLHR THLNPIRVNN STGFSYPLSL RKNKSFDRLV
60 70 80 90 100
CFAVDDEIRE KQQQQLSTSS TRLGSAVEER PENKDMIDGI SEEALENFKE
110 120 130 140 150
DGERSAIYDF LYPSKELLPD DKEMSIFDHL EELRERIFIS VLGVGGSILG
160 170 180 190 200
CFAFSKDLVK ILEAPVKSEG VRFLQLAPGE FFFTTLKVSG YCGLLLGSPI
210 220 230 240 250
ILYEIIAFII PGLTKEERKF LGPIVLGSSV LFYAGITFSY LVLVPAALNF
260 270 280 290 300
FVNYAEGAVE SLWSIDQYFE FVLVLMFSTG LSFQVPIIQL LLGQLGLVSG
310 320 330 340 350
DKMLSVWRYV VVGAVVAAAV VTPSTDPLTQ VLLAAPLLGL YLGGAWMVKL

AGR
Length:353
Mass (Da):38,912
Last modified:December 1, 2001 - v1
Checksum:iC08ED36571E6E2FB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF284759 mRNA. Translation: AAK93948.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF284759 mRNA. Translation: AAK93948.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

HAMAPiMF_00902. TatC.
InterProiIPR019820. Sec-indep_translocase_CS.
IPR002033. TatC.
[Graphical view]
PfamiPF00902. TatC. 1 hit.
[Graphical view]
PRINTSiPR01840. TATCFAMILY.
TIGRFAMsiTIGR00945. tatC. 1 hit.
PROSITEiPS01218. TATC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Chloroplast TatC plays a direct role in thylakoid (Delta)pH-dependent protein transport."
    Mori H., Summer E.J., Cline K.
    FEBS Lett. 501:65-68(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TOPOLOGY.
  2. "Thylakoid DeltapH-dependent precursor proteins bind to a cpTatC-Hcf106 complex before Tha4-dependent transport."
    Cline K., Mori H.
    J. Cell Biol. 154:719-729(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT.
  3. "A twin arginine signal peptide and the pH gradient trigger reversible assembly of the thylakoid [Delta]pH/Tat translocase."
    Mori H., Cline K.
    J. Cell Biol. 157:205-210(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT.
  4. "Functional assembly of thylakoid deltapH-dependent/Tat protein transport pathway components in vitro."
    Fincher V., Dabney-Smith C., Cline K.
    Eur. J. Biochem. 270:4930-4941(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT, SUBCELLULAR LOCATION.
  5. "Localization and integration of thylakoid protein translocase subunit cpTatC."
    Martin J.R., Harwood J.H., McCaffery M., Fernandez D.E., Cline K.
    Plant J. 58:831-842(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, SUBUNIT.
  6. "The chloroplast twin arginine transport (Tat) component, Tha4, undergoes conformational changes leading to Tat protein transport."
    Aldridge C., Storm A., Cline K., Dabney-Smith C.
    J. Biol. Chem. 287:34752-34763(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY.
  7. "Stoichiometry for binding and transport by the twin arginine translocation system."
    Celedon J.M., Cline K.
    J. Cell Biol. 197:523-534(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, SUBUNIT.

Entry informationi

Entry nameiTATC_PEA
AccessioniPrimary (citable) accession number: Q94G17
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2012
Last sequence update: December 1, 2001
Last modified: October 1, 2014
This is version 33 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

TATC targets to thylakoids via a stromal intermediate, and then probably integrated through a thylakoid translocation pathway.1 Publication

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.