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Protein

Salutaridinol 7-O-acetyltransferase

Gene

SALAT

Organism
Papaver somniferum (Opium poppy)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Involved in biosynthesis of morphinan-type benzylisoquinoline alkaloids. Catalyzes the conversion of the phenanthrene alkaloid salutaridinol to salutaridinol-7-O-acetate, the immediate precursor of thebaine along the morphine biosynthetic pathway. Conversion of 7-O-acetylsalutaridinol into thebaine is spontaneous.1 Publication

Catalytic activityi

Acetyl-CoA + salutaridinol = CoA + 7-O-acetylsalutaridinol.2 Publications

Kineticsi

  1. KM=9 µM for salutaridinol1 Publication
  2. KM=54 µM for acetyl-CoA1 Publication
  1. Vmax=25 pmol/sec/mg enzyme1 Publication

pH dependencei

Optimum pH is 7-9.1 Publication

Temperature dependencei

Optimum temperature is 47 degrees Celsius.1 Publication

Pathwayi: morphine biosynthesis

This protein is involved in the pathway morphine biosynthesis, which is part of Alkaloid biosynthesis.
View all proteins of this organism that are known to be involved in the pathway morphine biosynthesis and in Alkaloid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei163 – 1631Proton acceptorSequence analysis
Active sitei416 – 4161Proton acceptorSequence analysis

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Alkaloid metabolism

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-12301.
BRENDAi2.3.1.150. 4515.
UniPathwayiUPA00852.

Names & Taxonomyi

Protein namesi
Recommended name:
Salutaridinol 7-O-acetyltransferase1 Publication (EC:2.3.1.1502 Publications)
Short name:
salAT1 Publication
Gene namesi
Name:SALAT1 Publication
OrganismiPapaver somniferum (Opium poppy)
Taxonomic identifieri3469 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsRanunculalesPapaveraceaePapaveroideaePapaver

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 474474Salutaridinol 7-O-acetyltransferasePRO_0000147366Add
BLAST

Expressioni

Tissue specificityi

Expressed in root, stem, leaf and capsule of the mature plant (PubMed:11404355, PubMed:16813579). Restricted to sieve elements of the phloem adjacent or proximal to laticifers (PubMed:16813579).2 Publications

Developmental stagei

Increases rapidly between 1 and 3 days after seed germination.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ94FT4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the plant acyltransferase family.Curated

Phylogenomic databases

KOiK13393.

Family and domain databases

Gene3Di3.30.559.10. 2 hits.
InterProiIPR023213. CAT-like_dom.
IPR003480. Transferase.
[Graphical view]
PfamiPF02458. Transferase. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q94FT4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATMYSAAVE VISKETIKPT TPTPSQLKNF NLSLLDQCFP LYYYVPIILF
60 70 80 90 100
YPATAANSTG SSNHHDDLDL LKSSLSKTLV HFYPMAGRMI DNILVDCHDQ
110 120 130 140 150
GINFYKVKIR GKMCEFMSQP DVPLSQLLPS EVVSASVPKE ALVIVQVNMF
160 170 180 190 200
DCGGTAICSS VSHKIADAAT MSTFIRSWAS TTKTSRSGGS TAAVTDQKLI
210 220 230 240 250
PSFDSASLFP PSERLTSPSG MSEIPFSSTP EDTEDDKTVS KRFVFDFAKI
260 270 280 290 300
TSVREKLQVL MHDNYKSRRQ TRVEVVTSLI WKSVMKSTPA GFLPVVHHAV
310 320 330 340 350
NLRKKMDPPL QDVSFGNLSV TVSAFLPATT TTTTNAVNKT INSTSSESQV
360 370 380 390 400
VLHELHDFIA QMRSEIDKVK GDKGSLEKVI QNFASGHDAS IKKINDVEVI
410 420 430 440 450
NFWISSWCRM GLYEIDFGWG KPIWVTVDPN IKPNKNCFFM NDTKCGEGIE
460 470
VWASFLEDDM AKFELHLSEI LELI
Length:474
Mass (Da):52,632
Last modified:December 1, 2001 - v1
Checksum:i3A467FEE8B6A155E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF339913 mRNA. Translation: AAK73661.1.

Genome annotation databases

KEGGiag:AAK73661.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF339913 mRNA. Translation: AAK73661.1.

3D structure databases

ProteinModelPortaliQ94FT4.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGiag:AAK73661.

Phylogenomic databases

KOiK13393.

Enzyme and pathway databases

UniPathwayiUPA00852.
BioCyciMetaCyc:MONOMER-12301.
BRENDAi2.3.1.150. 4515.

Family and domain databases

Gene3Di3.30.559.10. 2 hits.
InterProiIPR023213. CAT-like_dom.
IPR003480. Transferase.
[Graphical view]
PfamiPF02458. Transferase. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular characterization of the salutaridinol 7-O-acetyltransferase involved in morphine biosynthesis in Opium poppy Papaver somniferum."
    Grothe T., Lenz R., Kutchan T.M.
    J. Biol. Chem. 276:30717-30723(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 14-30; 241-244; 257-272; 287-313; 340-362 AND 378-394, FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
  2. "Acetyl coenzyme A:salutaridinol-7-O-acetyltransferase from Papaver somniferum plant cell cultures. The enzyme catalyzing the formation of thebaine in morphine biosynthesis."
    Lenz R., Zenk M.H.
    J. Biol. Chem. 270:31091-31096(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: CATALYTIC ACTIVITY.
  3. "The role of phloem sieve elements and laticifers in the biosynthesis and accumulation of alkaloids in opium poppy."
    Samanani N., Alcantara J., Bourgault R., Zulak K.G., Facchini P.J.
    Plant J. 47:547-563(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Strain: cv. Marianne.

Entry informationi

Entry nameiSALAT_PAPSO
AccessioniPrimary (citable) accession number: Q94FT4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: December 1, 2001
Last modified: December 9, 2015
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.