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Q94FT4

- SALAT_PAPSO

UniProt

Q94FT4 - SALAT_PAPSO

Protein

Salutaridinol 7-O-acetyltransferase

Gene

SALAT

Organism
Papaver somniferum (Opium poppy)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 40 (01 Oct 2014)
      Sequence version 1 (01 Dec 2001)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of the phenanthrene alkaloid salutaridinol to salutaridinol-7-O-acetate, the immediate precursor of thebaine along the morphine biosynthetic pathway. Conversion of 7-O-acetylsalutaridinol into thebaine is spontaneous.1 Publication

    Catalytic activityi

    Acetyl-CoA + salutaridinol = CoA + 7-O-acetylsalutaridinol.1 Publication

    Kineticsi

    1. KM=9 µM for salutaridinol1 Publication
    2. KM=54 µM for acetyl-CoA1 Publication

    Vmax=25 pmol/sec/mg enzyme1 Publication

    pH dependencei

    Optimum pH is 7-9.1 Publication

    Temperature dependencei

    Optimum temperature is 47 degrees Celsius.1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei163 – 1631Proton acceptorSequence Analysis
    Active sitei416 – 4161Proton acceptorSequence Analysis

    GO - Molecular functioni

    1. salutaridinol 7-O-acetyltransferase activity Source: UniProtKB-EC

    GO - Biological processi

    1. alkaloid metabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Keywords - Biological processi

    Alkaloid metabolism

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-12301.
    BRENDAi2.3.1.150. 4515.
    UniPathwayiUPA00852.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Salutaridinol 7-O-acetyltransferase (EC:2.3.1.150)
    Short name:
    salAT
    Gene namesi
    Name:SALAT
    OrganismiPapaver somniferum (Opium poppy)
    Taxonomic identifieri3469 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsRanunculalesPapaveraceaePapaveroideaePapaver

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 474474Salutaridinol 7-O-acetyltransferasePRO_0000147366Add
    BLAST

    Expressioni

    Tissue specificityi

    Expressed in root, stem, leaf and capsule of the mature plant.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliQ94FT4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the plant acyltransferase family.Curated

    Family and domain databases

    Gene3Di3.30.559.10. 2 hits.
    InterProiIPR023213. CAT-like_dom.
    IPR003480. Transferase.
    [Graphical view]
    PfamiPF02458. Transferase. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q94FT4-1 [UniParc]FASTAAdd to Basket

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    MATMYSAAVE VISKETIKPT TPTPSQLKNF NLSLLDQCFP LYYYVPIILF    50
    YPATAANSTG SSNHHDDLDL LKSSLSKTLV HFYPMAGRMI DNILVDCHDQ 100
    GINFYKVKIR GKMCEFMSQP DVPLSQLLPS EVVSASVPKE ALVIVQVNMF 150
    DCGGTAICSS VSHKIADAAT MSTFIRSWAS TTKTSRSGGS TAAVTDQKLI 200
    PSFDSASLFP PSERLTSPSG MSEIPFSSTP EDTEDDKTVS KRFVFDFAKI 250
    TSVREKLQVL MHDNYKSRRQ TRVEVVTSLI WKSVMKSTPA GFLPVVHHAV 300
    NLRKKMDPPL QDVSFGNLSV TVSAFLPATT TTTTNAVNKT INSTSSESQV 350
    VLHELHDFIA QMRSEIDKVK GDKGSLEKVI QNFASGHDAS IKKINDVEVI 400
    NFWISSWCRM GLYEIDFGWG KPIWVTVDPN IKPNKNCFFM NDTKCGEGIE 450
    VWASFLEDDM AKFELHLSEI LELI 474
    Length:474
    Mass (Da):52,632
    Last modified:December 1, 2001 - v1
    Checksum:i3A467FEE8B6A155E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF339913 mRNA. Translation: AAK73661.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF339913 mRNA. Translation: AAK73661.1 .

    3D structure databases

    ProteinModelPortali Q94FT4.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00852 .
    BioCyci MetaCyc:MONOMER-12301.
    BRENDAi 2.3.1.150. 4515.

    Family and domain databases

    Gene3Di 3.30.559.10. 2 hits.
    InterProi IPR023213. CAT-like_dom.
    IPR003480. Transferase.
    [Graphical view ]
    Pfami PF02458. Transferase. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular characterization of the salutaridinol 7-O-acetyltransferase involved in morphine biosynthesis in Opium poppy Papaver somniferum."
      Grothe T., Lenz R., Kutchan T.M.
      J. Biol. Chem. 276:30717-30723(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 14-30; 241-244; 257-272; 287-313; 340-362 AND 378-394, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
    2. "Acetyl coenzyme A:salutaridinol-7-O-acetyltransferase from Papaver somniferum plant cell cultures. The enzyme catalyzing the formation of thebaine in morphine biosynthesis."
      Lenz R., Zenk M.H.
      J. Biol. Chem. 270:31091-31096(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: CATALYTIC ACTIVITY.

    Entry informationi

    Entry nameiSALAT_PAPSO
    AccessioniPrimary (citable) accession number: Q94FT4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 19, 2004
    Last sequence update: December 1, 2001
    Last modified: October 1, 2014
    This is version 40 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3