Q94FT4 (SALAT_PAPSO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Salutaridinol 7-O-acetyltransferase
Short name=salAT
EC=2.3.1.150

Gene names

Name:

SALAT

Organism

Papaver somniferum (Opium poppy)

Taxonomic identifier

3469 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › stem eudicotyledons › Ranunculales › Papaveraceae › Papaver

Protein attributes

Sequence length	474 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the conversion of the phenanthrene alkaloid salutaridinol to salutaridinol-7-O-acetate, the immediate precursor of thebaine along the morphine biosynthetic pathway. Conversion of 7-O-acetylsalutaridinol into thebaine is spontaneous. Ref.1
Catalytic activity	Acetyl-CoA + salutaridinol = CoA + 7-O-acetylsalutaridinol. Ref.2
Pathway	Alkaloid biosynthesis; morphine biosynthesis.
Tissue specificity	Expressed in root, stem, leaf and capsule of the mature plant. Ref.1
Sequence similarities	Belongs to the plant acyltransferase family.
Biophysicochemical properties	Kinetic parameters: KM=9 µM for salutaridinol Ref.1 KM=54 µM for acetyl-CoA Vmax=25 pmol/sec/mg enzyme pH dependence: Optimum pH is 7-9. Temperature dependence: Optimum temperature is 47 degrees Celsius.

Ontologies

Keywords
Biological process	Alkaloid metabolism
Molecular function	Acyltransferase Transferase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	alkaloid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	salutaridinol 7-O-acetyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 474	474	Salutaridinol 7-O-acetyltransferase		PRO_0000147366

Sequences

	Sequence		Length	Mass (Da)
	Q94FT4 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 3A467FEE8B6A155E	FASTA	474	52,632
	10 20 30 40 50 60 MATMYSAAVE VISKETIKPT TPTPSQLKNF NLSLLDQCFP LYYYVPIILF YPATAANSTG 70 80 90 100 110 120 SSNHHDDLDL LKSSLSKTLV HFYPMAGRMI DNILVDCHDQ GINFYKVKIR GKMCEFMSQP 130 140 150 160 170 180 DVPLSQLLPS EVVSASVPKE ALVIVQVNMF DCGGTAICSS VSHKIADAAT MSTFIRSWAS 190 200 210 220 230 240 TTKTSRSGGS TAAVTDQKLI PSFDSASLFP PSERLTSPSG MSEIPFSSTP EDTEDDKTVS 250 260 270 280 290 300 KRFVFDFAKI TSVREKLQVL MHDNYKSRRQ TRVEVVTSLI WKSVMKSTPA GFLPVVHHAV 310 320 330 340 350 360 NLRKKMDPPL QDVSFGNLSV TVSAFLPATT TTTTNAVNKT INSTSSESQV VLHELHDFIA 370 380 390 400 410 420 QMRSEIDKVK GDKGSLEKVI QNFASGHDAS IKKINDVEVI NFWISSWCRM GLYEIDFGWG 430 440 450 460 470 KPIWVTVDPN IKPNKNCFFM NDTKCGEGIE VWASFLEDDM AKFELHLSEI LELI

References

[1]

"Molecular characterization of the salutaridinol 7-O-acetyltransferase involved in morphine biosynthesis in Opium poppy Papaver somniferum." Grothe T., Lenz R., Kutchan T.M. J. Biol. Chem. 276:30717-30723(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 14-30; 241-244; 257-272; 287-313; 340-362 AND 378-394, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.

[2]

"Acetyl coenzyme A:salutaridinol-7-O-acetyltransferase from Papaver somniferum plant cell cultures. The enzyme catalyzing the formation of thebaine in morphine biosynthesis." Lenz R., Zenk M.H. J. Biol. Chem. 270:31091-31096(1995) [PubMed] [Europe PMC] [Abstract] Cited for: CATALYTIC ACTIVITY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF339913 mRNA. Translation: AAK73661.1.
3D structure databases
ProteinModelPortal	Q94FT4.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-12301.
BRENDA	2.3.1.150. 4515.
UniPathway	UPA00852.
Family and domain databases
Gene3D	3.30.559.10. 2 hits.
InterPro	IPR023213. CAT-like_dom. IPR003480. Transferase. [Graphical view]
Pfam	PF02458. Transferase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SALAT_PAPSO

Accession

Primary (citable) accession number: Q94FT4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2004
Last sequence update:	December 1, 2001
Last modified:	April 3, 2013
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program