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Protein

5-epiaristolochene 1,3-dihydroxylase

Gene

CYP71D20

Organism
Nicotiana tabacum (Common tobacco)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the biosynthesis of capsidiol. Catalyzes the successive and independent hydroxylations at the C1 and C3 positions of 5-epiaristolochene. The second hydroxylation step is 8-fold more efficient than the first hydroxylation reaction. Capable of utilizing premnaspirodiene as a substrate.2 Publications

Catalytic activityi

5-epiaristolochene + 2 NADPH + 2 O2 = capsidiol + 2 NADP+ + 2 H2O.3 Publications

Cofactori

Enzyme regulationi

Inhibited by ancymidol and ketoconazole.1 Publication

Kineticsi

  1. KM=19.18 µM for 5-epiaristolochene1 Publication
  2. KM=6.38 µM for 1-deoxycapsidiol1 Publication
  3. KM=1.74 µM for 3-deoxycapsidiol1 Publication

    pH dependencei

    Optimum pH is 7.5.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Metal bindingi442Iron (heme axial ligand)By similarity1

    GO - Molecular functioni

    Complete GO annotation...

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Ligandi

    Heme, Iron, Metal-binding, NADP

    Enzyme and pathway databases

    BioCyciMetaCyc:EAH-MONOMER.
    BRENDAi1.14.13.119. 3645.
    SABIO-RKQ94FM7.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    5-epiaristolochene 1,3-dihydroxylase (EC:1.14.13.119)
    Short name:
    NtEAH
    Alternative name(s):
    Cytochrome P450 71D20
    Gene namesi
    Name:CYP71D20
    OrganismiNicotiana tabacum (Common tobacco)
    Taxonomic identifieri4097 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana

    Subcellular locationi

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Transmembranei2 – 22HelicalSequence analysisAdd BLAST21

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi368S → A or T: Decreased substrate affinity but catalytic activities unchanged. 1 Publication1
    Mutagenesisi368S → C or V: Decreased synthesis of 3-deoxycapsidiol and loss of production of capsidiol. 1 Publication1
    Mutagenesisi368S → I or F: Loss of both catalytic activities. 1 Publication1
    Mutagenesisi482S → V: Loss of activity toward premnaspirodiene. 1 Publication1
    Mutagenesisi484I → V: Loss of activity toward premnaspirodiene. 1 Publication1
    Mutagenesisi486I → A: Decreased synthesis of 3-deoxycapsidiol and near loss of production of capsidiol. Loss of activity toward premnaspirodiene. 2 Publications1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00004094701 – 5045-epiaristolochene 1,3-dihydroxylaseAdd BLAST504

    Expressioni

    Inductioni

    Up-regulated 6 to 9 hours after elicitor-treatment and then declines by 12 hours.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliQ94FM7.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    KOiK15805.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PRINTSiPR00463. EP450I.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q94FM7-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MQFFSLVSIF LFLSFLFLLR KWKNSNSQSK KLPPGPWKIP ILGSMLHMIG
    60 70 80 90 100
    GEPHHVLRDL AKKYGPLMHL QLGEISAVVV TSRDMAKEVL KTHDVVFASR
    110 120 130 140 150
    PKIVAMDIIC YNQSDIAFSP YGDHWRQMRK ICVMELLNAK NVRSFSSIRR
    160 170 180 190 200
    DEVVRLIDSI RSDSSSGELV NFTQRIIWFA SSMTCRSAFG QVLKGQDIFA
    210 220 230 240 250
    KKIREVIGLA EGFDVVDIFP TYKFLHVLSG MKRKLLNAHL KVDAIVEDVI
    260 270 280 290 300
    NEHKKNLAAG KSNGALGGED LIDVLLRLMN DTSLQFPITN DNIKAVIVDM
    310 320 330 340 350
    FAAGTETSST TTVWAMAEMM KNPSVFTKAQ AEVREAFRDK VSFDENDVEE
    360 370 380 390 400
    LKYLKLVIKE TLRLHPPSPL LVPRECREDT DINGYTIPAK TKVMVNVWAL
    410 420 430 440 450
    GRDPKYWDDA ESFKPERFEQ CSVDFFGNNF EFLPFGGGRR ICPGMSFGLA
    460 470 480 490 500
    NLYLPLAQLL YHFDWKLPTG IMPRDLDLTE LSGITIARKG GLYLNATPYQ

    PSRE
    Length:504
    Mass (Da):57,083
    Last modified:October 25, 2005 - v2
    Checksum:i0E5B01665C0D3D44
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF368376 mRNA. Translation: AAK62342.2.
    RefSeqiNP_001311564.1. NM_001324635.1.
    UniGeneiNta.4312.

    Genome annotation databases

    GeneIDi107759261.
    KEGGiag:AAK62342.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF368376 mRNA. Translation: AAK62342.2.
    RefSeqiNP_001311564.1. NM_001324635.1.
    UniGeneiNta.4312.

    3D structure databases

    ProteinModelPortaliQ94FM7.
    ModBaseiSearch...
    MobiDBiSearch...

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    GeneIDi107759261.
    KEGGiag:AAK62342.

    Phylogenomic databases

    KOiK15805.

    Enzyme and pathway databases

    BioCyciMetaCyc:EAH-MONOMER.
    BRENDAi1.14.13.119. 3645.
    SABIO-RKQ94FM7.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PRINTSiPR00463. EP450I.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiC71DK_TOBAC
    AccessioniPrimary (citable) accession number: Q94FM7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 31, 2011
    Last sequence update: October 25, 2005
    Last modified: October 5, 2016
    This is version 69 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.