Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q94F88

- CMT3_ARATH

UniProt

Q94F88 - CMT3_ARATH

Protein

DNA (cytosine-5)-methyltransferase CMT3

Gene

CMT3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 2 (25 Jul 2006)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Involved in the CpXpG methylation and in gene silencing. Methylates preferentially transposon-related sequences. Functionally redundant to DRM1/DRM2 to maintain non-CpG methylation. Involved in RNA-directed DNA methylation.4 Publications

    Catalytic activityi

    S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei460 – 4601PROSITE-ProRule annotation

    GO - Molecular functioni

    1. chromatin binding Source: InterPro
    2. DNA (cytosine-5-)-methyltransferase activity Source: TAIR
    3. DNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. C-5 methylation of cytosine Source: GOC
    2. chromatin silencing Source: TAIR
    3. DNA methylation Source: TAIR
    4. DNA methylation on cytosine within a CNG sequence Source: TAIR
    5. histone H3-K9 methylation Source: TAIR
    6. negative regulation of gene expression, epigenetic Source: TAIR
    7. transcription, DNA-templated Source: UniProtKB-KW
    8. zygote asymmetric cytokinesis in embryo sac Source: TAIR

    Keywords - Molecular functioni

    Chromatin regulator, Methyltransferase, Transferase

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciARA:AT1G69770-MONOMER.

    Protein family/group databases

    REBASEi4853. M.AthCMT3.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA (cytosine-5)-methyltransferase CMT3 (EC:2.1.1.37)
    Alternative name(s):
    Chromomethylase 3
    Protein CHROMOMETHYLASE 3
    Gene namesi
    Name:CMT3
    Ordered Locus Names:At1g69770
    ORF Names:T6C23.3
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G69770.

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi279 – 2791G → E: Loss of activity. 1 Publication
    Mutagenesisi382 – 3821F → A: Loss of binding to H3. 1 Publication
    Mutagenesisi456 – 4561G → D: Loss of activity. 1 Publication
    Mutagenesisi465 – 4651G → D: Loss of activity. 1 Publication
    Mutagenesisi470 – 4701R → K in cmt3-10; partial loss of activity. 1 Publication
    Mutagenesisi541 – 5411G → E in cmt3-6; loss of activity. 1 Publication
    Mutagenesisi542 – 5421L → F: Loss of activity. 1 Publication
    Mutagenesisi683 – 6831R → K: Loss of activity. 1 Publication
    Mutagenesisi724 – 7241G → E in cmt3-4; loss of activity. 1 Publication
    Mutagenesisi733 – 7331L → F: Loss of activity. 1 Publication
    Mutagenesisi748 – 7481P → L: Loss of activity. 1 Publication
    Mutagenesisi763 – 7631S → F in cmt3-8; partial loss of activity. 1 Publication
    Mutagenesisi769 – 7691R → K in cmt3-3; loss of activity. 1 Publication
    Mutagenesisi807 – 8071G → R: Loss of activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 839839DNA (cytosine-5)-methyltransferase CMT3PRO_0000246693Add
    BLAST

    Proteomic databases

    PaxDbiQ94F88.
    PRIDEiQ94F88.

    Expressioni

    Gene expression databases

    ArrayExpressiQ94F88.
    GenevestigatoriQ94F88.

    Interactioni

    Subunit structurei

    Homodimer. Interacts with HP1 and, through its chromodomain, with the N-terminal tail of histone H3 doubly methylated at 'Lys-9' and 'Lys-27'.2 Publications

    Protein-protein interaction databases

    BioGridi28534. 1 interaction.
    DIPiDIP-60719N.

    Structurei

    3D structure databases

    ProteinModelPortaliQ94F88.
    SMRiQ94F88. Positions 46-827.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini108 – 227120BAHPROSITE-ProRule annotationAdd
    BLAST
    Domaini269 – 813545SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd
    BLAST
    Domaini382 – 44766ChromoPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation
    Contains 1 BAH domain.PROSITE-ProRule annotation
    Contains 1 chromo domain.PROSITE-ProRule annotation
    Contains 1 SAM-dependent MTase C5-type domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0270.
    HOGENOMiHOG000082844.
    InParanoidiQ94F88.
    KOiK00558.
    OMAiHRPLELN.
    PhylomeDBiQ94F88.

    Family and domain databases

    Gene3Di3.40.50.150. 2 hits.
    InterProiIPR001025. BAH_dom.
    IPR018117. C5_DNA_meth_AS.
    IPR001525. C5_MeTfrase.
    IPR025821. C5_MeTfrase_pln.
    IPR023780. Chromo_domain.
    IPR000953. Chromo_domain/shadow.
    IPR016197. Chromodomain-like.
    IPR023779. Chromodomain_CS.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PANTHERiPTHR10629. PTHR10629. 1 hit.
    PfamiPF01426. BAH. 1 hit.
    PF00385. Chromo. 1 hit.
    PF00145. DNA_methylase. 1 hit.
    [Graphical view]
    PRINTSiPR00105. C5METTRFRASE.
    SMARTiSM00439. BAH. 1 hit.
    SM00298. CHROMO. 1 hit.
    [Graphical view]
    SUPFAMiSSF53335. SSF53335. 4 hits.
    SSF54160. SSF54160. 1 hit.
    TIGRFAMsiTIGR00675. dcm. 1 hit.
    PROSITEiPS51038. BAH. 1 hit.
    PS00094. C5_MTASE_1. 1 hit.
    PS00598. CHROMO_1. 1 hit.
    PS50013. CHROMO_2. 1 hit.
    PS51679. SAM_MT_C5. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q94F88-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAPKRKRPAT KDDTTKSIPK PKKRAPKRAK TVKEEPVTVV EEGEKHVARF    50
    LDEPIPESEA KSTWPDRYKP IEVQPPKASS RKKTKDDEKV EIIRARCHYR 100
    RAIVDERQIY ELNDDAYVQS GEGKDPFICK IIEMFEGANG KLYFTARWFY 150
    RPSDTVMKEF EILIKKKRVF FSEIQDTNEL GLLEKKLNIL MIPLNENTKE 200
    TIPATENCDF FCDMNYFLPY DTFEAIQQET MMAISESSTI SSDTDIREGA 250
    AAISEIGECS QETEGHKKAT LLDLYSGCGA MSTGLCMGAQ LSGLNLVTKW 300
    AVDMNAHACK SLQHNHPETN VRNMTAEDFL FLLKEWEKLC IHFSLRNSPN 350
    SEEYANLHGL NNVEDNEDVS EESENEDDGE VFTVDKIVGI SFGVPKKLLK 400
    RGLYLKVRWL NYDDSHDTWE PIEGLSNCRG KIEEFVKLGY KSGILPLPGG 450
    VDVVCGGPPC QGISGHNRFR NLLDPLEDQK NKQLLVYMNI VEYLKPKFVL 500
    MENVVDMLKM AKGYLARFAV GRLLQMNYQV RNGMMAAGAY GLAQFRLRFF 550
    LWGALPSEII PQFPLPTHDL VHRGNIVKEF QGNIVAYDEG HTVKLADKLL 600
    LKDVISDLPA VANSEKRDEI TYDKDPTTPF QKFIRLRKDE ASGSQSKSKS 650
    KKHVLYDHHP LNLNINDYER VCQVPKRKGA NFRDFPGVIV GPGNVVKLEE 700
    GKERVKLESG KTLVPDYALT YVDGKSCKPF GRLWWDEIVP TVVTRAEPHN 750
    QVIIHPEQNR VLSIRENARL QGFPDDYKLF GPPKQKYIQV GNAVAVPVAK 800
    ALGYALGTAF QGLAVGKDPL LTLPEGFAFM KPTLPSELA 839
    Length:839
    Mass (Da):94,905
    Last modified:July 25, 2006 - v2
    Checksum:iB1B4D5F7AE0A3AAC
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti138 – 1381A → V in AAK71870. (PubMed:11349138)Curated
    Sequence conflicti165 – 1651K → N in AAK71870. (PubMed:11349138)Curated
    Sequence conflicti166 – 1661K → E in AAK69756. (PubMed:11459824)Curated
    Sequence conflicti266 – 2683HKK → QKE in AAK71870. (PubMed:11349138)Curated
    Sequence conflicti433 – 4331E → G in AAK71870. (PubMed:11349138)Curated
    Sequence conflicti821 – 8222LT → II in AAK71870. (PubMed:11349138)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC013289 Genomic DNA. Translation: AAG52543.1.
    CP002684 Genomic DNA. Translation: AEE34973.1.
    AF383170 Genomic DNA. Translation: AAK69756.1.
    AF364174 Genomic DNA. Translation: AAK71870.1.
    PIRiG96719.
    RefSeqiNP_177135.1. NM_105645.3.
    UniGeneiAt.35376.

    Genome annotation databases

    EnsemblPlantsiAT1G69770.1; AT1G69770.1; AT1G69770.
    GeneIDi843313.
    KEGGiath:AT1G69770.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC013289 Genomic DNA. Translation: AAG52543.1 .
    CP002684 Genomic DNA. Translation: AEE34973.1 .
    AF383170 Genomic DNA. Translation: AAK69756.1 .
    AF364174 Genomic DNA. Translation: AAK71870.1 .
    PIRi G96719.
    RefSeqi NP_177135.1. NM_105645.3.
    UniGenei At.35376.

    3D structure databases

    ProteinModelPortali Q94F88.
    SMRi Q94F88. Positions 46-827.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 28534. 1 interaction.
    DIPi DIP-60719N.

    Protein family/group databases

    REBASEi 4853. M.AthCMT3.

    Proteomic databases

    PaxDbi Q94F88.
    PRIDEi Q94F88.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G69770.1 ; AT1G69770.1 ; AT1G69770 .
    GeneIDi 843313.
    KEGGi ath:AT1G69770.

    Organism-specific databases

    TAIRi AT1G69770.

    Phylogenomic databases

    eggNOGi COG0270.
    HOGENOMi HOG000082844.
    InParanoidi Q94F88.
    KOi K00558.
    OMAi HRPLELN.
    PhylomeDBi Q94F88.

    Enzyme and pathway databases

    BioCyci ARA:AT1G69770-MONOMER.

    Gene expression databases

    ArrayExpressi Q94F88.
    Genevestigatori Q94F88.

    Family and domain databases

    Gene3Di 3.40.50.150. 2 hits.
    InterProi IPR001025. BAH_dom.
    IPR018117. C5_DNA_meth_AS.
    IPR001525. C5_MeTfrase.
    IPR025821. C5_MeTfrase_pln.
    IPR023780. Chromo_domain.
    IPR000953. Chromo_domain/shadow.
    IPR016197. Chromodomain-like.
    IPR023779. Chromodomain_CS.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    PANTHERi PTHR10629. PTHR10629. 1 hit.
    Pfami PF01426. BAH. 1 hit.
    PF00385. Chromo. 1 hit.
    PF00145. DNA_methylase. 1 hit.
    [Graphical view ]
    PRINTSi PR00105. C5METTRFRASE.
    SMARTi SM00439. BAH. 1 hit.
    SM00298. CHROMO. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53335. SSF53335. 4 hits.
    SSF54160. SSF54160. 1 hit.
    TIGRFAMsi TIGR00675. dcm. 1 hit.
    PROSITEi PS51038. BAH. 1 hit.
    PS00094. C5_MTASE_1. 1 hit.
    PS00598. CHROMO_1. 1 hit.
    PS50013. CHROMO_2. 1 hit.
    PS51679. SAM_MT_C5. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Arabidopsis cmt3 chromomethylase mutations block non-CG methylation and silencing of an endogenous gene."
      Bartee L., Malagnac F., Bender J.
      Genes Dev. 15:1753-1758(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF GLY-279; GLY-456; GLY-465; LEU-542; ARG-683; LEU-733; PRO-748 AND GLY-807, FUNCTION.
      Strain: cv. Wassilewskija.
    4. "Requirement of CHROMOMETHYLASE3 for maintenance of CpXpG methylation."
      Lindroth A.M., Cao X., Jackson J.P., Zilberman D., McCallum C.M., Henikoff S., Jacobsen S.E.
      Science 292:2077-2080(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF ARG-470; GLY-541; GLY-724; SER-763 AND ARG-769, FUNCTION.
      Strain: cv. Landsberg erecta.
    5. "Dual histone H3 methylation marks at lysines 9 and 27 required for interaction with CHROMOMETHYLASE3."
      Lindroth A.M., Shultis D., Jasencakova Z., Fuchs J., Johnson L., Schubert D., Patnaik D., Pradhan S., Goodrich J., Schubert I., Jenuwein T., Khorasanizadeh S., Jacobsen S.E.
      EMBO J. 23:4286-4296(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH H3, MUTAGENESIS OF PHE-382.
    6. "Control of CpNpG DNA methylation by the KRYPTONITE histone H3 methyltransferase."
      Jackson J.P., Lindroth A.M., Cao X., Jacobsen S.E.
      Nature 416:556-560(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH HP1.
    7. "Genome-wide profiling of DNA methylation reveals transposon targets of CHROMOMETHYLASE3."
      Tompa R., McCallum C.M., Delrow J., Henikoff J.G., van Steensel B., Henikoff S.
      Curr. Biol. 12:65-68(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    8. "Role of the DRM and CMT3 methyltransferases in RNA-directed DNA methylation."
      Cao X., Aufsatz W., Zilberman D., Mette M.F., Huang M.S., Matzke M., Jacobsen S.E.
      Curr. Biol. 13:2212-2217(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiCMT3_ARATH
    AccessioniPrimary (citable) accession number: Q94F88
    Secondary accession number(s): Q94FN4, Q9C9L8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 25, 2006
    Last sequence update: July 25, 2006
    Last modified: October 1, 2014
    This is version 94 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3