Reviewed,
UniProtKB/Swiss-Prot Q94F88 (CMT3_ARATH)
Last modified
November 3, 2009.
Version 54.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: DNA (cytosine-5)-methyltransferase CMT3 EC=2.1.1.37 Alternative name(s): Chromomethylase 3 Protein CHROMOMETHYLASE 3 | ||||||
| Gene names |
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| Organism | Arabidopsis thaliana (Mouse-ear cress) [Complete proteome] | ||||||
| Taxonomic identifier | 3702 [NCBI] | ||||||
| Taxonomic lineage | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis |
Protein attributes
| Sequence length | 839 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Involved in the CpXpG methylation and in gene silencing. Methylates preferentially transposon-related sequences. Functionally redundant to DRM1/DRM2 to maintain non-CpG methylation. Involved in RNA-directed DNA methylation. Ref.2 Ref.3 Ref.6 Ref.7 |
| Catalytic activity | S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine. |
| Subunit structure | Homodimer. Interacts with HP1 and, through its chromodomain, with the N-terminal tail of histone H3 doubly methylated at 'Lys-9' and 'Lys-27'. Ref.4 Ref.5 |
| Subcellular location | Nucleus By similarity. |
| Sequence similarities | Belongs to the C5-methyltransferase family. Contains 1 BAH domain. Contains 1 chromo domain. |
Ontologies
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 839 | 839 | DNA (cytosine-5)-methyltransferase CMT3 | PRO_0000246693 | |||||
Regions | |||||||||
| Domain | 108 – 227 | 120 | BAH | ||||||
| Domain | 382 – 447 | 66 | Chromo | ||||||
Experimental info | |||||||||
| Mutagenesis | 279 | 1 | G → E: Loss of activity. Ref.2 | ||||||
| Mutagenesis | 382 | 1 | F → A: Loss of binding to H3. Ref.4 | ||||||
| Mutagenesis | 456 | 1 | G → D: Loss of activity. Ref.2 | ||||||
| Mutagenesis | 465 | 1 | G → D: Loss of activity. Ref.2 | ||||||
| Mutagenesis | 470 | 1 | R → K in cmt3-10; partial loss of activity. Ref.3 | ||||||
| Mutagenesis | 541 | 1 | G → E in cmt3-6; loss of activity. Ref.3 | ||||||
| Mutagenesis | 542 | 1 | L → F: Loss of activity. Ref.2 | ||||||
| Mutagenesis | 683 | 1 | R → K: Loss of activity. Ref.2 | ||||||
| Mutagenesis | 724 | 1 | G → E in cmt3-4; loss of activity. Ref.3 | ||||||
| Mutagenesis | 733 | 1 | L → F: Loss of activity. Ref.2 | ||||||
| Mutagenesis | 748 | 1 | P → L: Loss of activity. Ref.2 | ||||||
| Mutagenesis | 763 | 1 | S → F in cmt3-8; partial loss of activity. Ref.3 | ||||||
| Mutagenesis | 769 | 1 | R → K in cmt3-3; loss of activity. Ref.3 | ||||||
| Mutagenesis | 807 | 1 | G → R: Loss of activity. Ref.2 | ||||||
| Sequence conflict | 138 | 1 | A → V in AAK71870. Ref.3 | ||||||
| Sequence conflict | 165 | 1 | K → N in AAK71870. Ref.3 | ||||||
| Sequence conflict | 166 | 1 | K → E in AAK69756. Ref.2 | ||||||
| Sequence conflict | 266 – 268 | 3 | HKK → QKE in AAK71870. Ref.3 | ||||||
| Sequence conflict | 433 | 1 | E → G in AAK71870. Ref.3 | ||||||
| Sequence conflict | 821 – 822 | 2 | LT → II in AAK71870. Ref.3 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. Davis R.W.Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia. |
| [2] | "Arabidopsis cmt3 chromomethylase mutations block non-CG methylation and silencing of an endogenous gene." Bartee L., Malagnac F., Bender J. Genes Dev. 15:1753-1758(2001) [PubMed: 11459824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF GLY-279; GLY-456; GLY-465; LEU-542; ARG-683; LEU-733; PRO-748 AND GLY-807, FUNCTION. Strain: cv. Wassilewskija. |
| [3] | "Requirement of CHROMOMETHYLASE3 for maintenance of CpXpG methylation." Lindroth A.M., Cao X., Jackson J.P., Zilberman D., McCallum C.M., Henikoff S., Jacobsen S.E. Science 292:2077-2080(2001) [PubMed: 11349138] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF ARG-470; GLY-541; GLY-724; SER-763 AND ARG-769, FUNCTION. Strain: cv. Landsberg erecta. |
| [4] | "Dual histone H3 methylation marks at lysines 9 and 27 required for interaction with CHROMOMETHYLASE3." Lindroth A.M., Shultis D., Jasencakova Z., Fuchs J., Johnson L., Schubert D., Patnaik D., Pradhan S., Goodrich J., Schubert I., Jenuwein T., Khorasanizadeh S., Jacobsen S.E. EMBO J. 23:4286-4296(2004) [PubMed: 15457214] [Abstract] Cited for: INTERACTION WITH H3, MUTAGENESIS OF PHE-382. |
| [5] | "Control of CpNpG DNA methylation by the KRYPTONITE histone H3 methyltransferase." Jackson J.P., Lindroth A.M., Cao X., Jacobsen S.E. Nature 416:556-560(2002) [PubMed: 11898023] [Abstract] Cited for: INTERACTION WITH HP1. |
| [6] | "Genome-wide profiling of DNA methylation reveals transposon targets of CHROMOMETHYLASE3." Tompa R., McCallum C.M., Delrow J., Henikoff J.G., van Steensel B., Henikoff S. Curr. Biol. 12:65-68(2002) [PubMed: 11790305] [Abstract] Cited for: FUNCTION. |
| [7] | "Role of the DRM and CMT3 methyltransferases in RNA-directed DNA methylation." Cao X., Aufsatz W., Zilberman D., Mette M.F., Huang M.S., Matzke M., Jacobsen S.E. Curr. Biol. 13:2212-2217(2003) [PubMed: 14680640] [Abstract] Cited for: FUNCTION. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| AC013289 Genomic DNA. Translation: AAG52543.1. AF383170 Genomic DNA. Translation: AAK69756.1. AF364174 Genomic DNA. Translation: AAK71870.1. | |
| IPI | IPI00534552. |
| PIR | G96719. |
| RefSeq | NP_177135.1. |
| UniGene | At.35376 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1G55 based on UniProtKB O14717. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | Q94F88. |
Proteomic databases | |
| PRIDE | Q94F88. |
Genome annotation databases | |
| GeneID | 843313. |
| GenomeReviews | Gene locus AT1G69770 in contig CT485782_GR. |
| KEGG | ath:AT1G69770. |
| NMPDR | fig|3702.1.peg.6449. |
Organism-specific databases | |
| TAIR | At1g69770. |
Phylogenomic databases | |
| OMA | HRPLELN. |
Enzyme and pathway databases | |
| BRENDA | 2.1.1.37. 302. |
Gene expression databases | |
| Genevestigator | Q94F88. |
| GermOnline | AT1G69770. Arabidopsis thaliana. |
Family and domain databases | |
| InterPro | IPR001025. BAH. IPR001525. C5_DNA_meth. IPR018117. C5_DNA_meth_AS. IPR000953. Chromodomain. [Graphical view] |
| PANTHER | PTHR10629. C5_DNA_meth. 1 hit. |
| Pfam | PF01426. BAH. 1 hit. PF00385. Chromo. 1 hit. PF00145. DNA_methylase. 1 hit. [Graphical view] |
| PRINTS | PR00105. C5METTRFRASE. |
| SMART | SM00439. BAH. 1 hit. SM00298. CHROMO. 1 hit. [Graphical view] |
| TIGRFAMs | TIGR00675. dcm. 1 hit. |
| PROSITE | PS51038. BAH. 1 hit. PS00094. C5_MTASE_1. 1 hit. PS00598. CHROMO_1. 1 hit. PS50013. CHROMO_2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | CMT3_ARATH | ||||||||
| Accession | Primary (citable) accession number: Q94F88 Secondary accession number(s): Q94FN4, Q9C9L8 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | PPAP (Plant Proteome Annotation Project) | ||||||||
Relevant documents
| Arabidopsis thaliana Arabidopsis thaliana: entries and gene names |
| SIMILARITY comments Index of protein domains and families |

Clusters with


