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Q94F87 (CMT2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA (cytosine-5)-methyltransferase CMT2

EC=2.1.1.37
Alternative name(s):
Chromomethylase 2
Protein CHROMOMETHYLASE 2
Gene names
Name:CMT2
Ordered Locus Names:At4g19020
ORF Names:F13C5.190
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length1295 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

May be involved in the CpXpG methylation and in gene silencing By similarity.

Catalytic activity

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.

Subcellular location

Nucleus By similarity.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.

Contains 1 BAH domain.

Contains 1 chromo domain.

Contains 1 SAM-dependent MTase C5-type domain.

Sequence caution

The sequence AAK69757.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence BX828439 differs from that shown. Reason: Erroneous termination at position 1226. Translated as Gln.

The sequence CAA16759.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence CAB78904.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 12951295DNA (cytosine-5)-methyltransferase CMT2
PRO_0000246692

Regions

Domain578 – 693116BAH
Domain727 – 1268542SAM-dependent MTase C5-type
Domain837 – 90266Chromo
Compositional bias822 – 8287Poly-Ser

Sites

Active site9151 By similarity

Experimental info

Sequence conflict54 – 552DN → ND in AAK69757. Ref.1
Sequence conflict110 – 1112AL → PV in AAK69757. Ref.1
Sequence conflict1251K → E in AAK69757. Ref.1
Sequence conflict132 – 1332KF → NS in AAK69757. Ref.1
Sequence conflict1561F → S in AAK69757. Ref.1
Sequence conflict2291R → K in AAK69757. Ref.1
Sequence conflict3401K → N in AAK69757. Ref.1
Sequence conflict5011K → N in AAK69757. Ref.1
Sequence conflict6651V → A in AAK69757. Ref.1
Sequence conflict7051C → W in AAK69757. Ref.1
Sequence conflict8231G → E in AAK69757. Ref.1
Sequence conflict8501H → P in AAK69757. Ref.1
Sequence conflict11321K → N in AAK69757. Ref.1
Sequence conflict11441L → I in AAK69757. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q94F87 [UniParc].

Last modified February 8, 2011. Version 3.
Checksum: 33CE317C541825A6

FASTA1,295145,015
        10         20         30         40         50         60 
MLSPAKCESE EAQAPLDLHS SSRSEPECLS LVLWCPNPEE AAPSSTRELI KLPDNGEMSL 

        70         80         90        100        110        120 
RRSTTLNCNS PEENGGEGRV SQRKSSRGKS QPLLMLTNGC QLRRSPRFRA LHANFDNVCS 

       130        140        150        160        170        180 
VPVTKGGVSQ RKFSRGKSQP LLTLTNGCQL RRSPRFRAVD GNFDSVCSVP VTGKFGSRKR 

       190        200        210        220        230        240 
KSNSALDKKE SSDSEGLTFK DIAVIAKSLE MEIISECQYK NNVAEGRSRL QDPAKRKVDS 

       250        260        270        280        290        300 
DTLLYSSINS SKQSLGSNKR MRRSQRFMKG TENEGEENLG KSKGKGMSLA SCSFRRSTRL 

       310        320        330        340        350        360 
SGTVETGNTE TLNRRKDCGP ALCGAEQVRG TERLVQISKK DHCCEAMKKC EGDGLVSSKQ 

       370        380        390        400        410        420 
ELLVFPSGCI KKTVNGCRDR TLGKPRSSGL NTDDIHTSSL KISKNDTSNG LTMTTALVEQ 

       430        440        450        460        470        480 
DAMESLLQGK TSACGAADKG KTREMHVNST VIYLSDSDEP SSIEYLNGDN LTQVESGSAL 

       490        500        510        520        530        540 
SSGGNEGIVS LDLNNPTKST KRKGKRVTRT AVQEQNKRSI CFFIGEPLSC EEAQERWRWR 

       550        560        570        580        590        600 
YELKERKSKS RGQQSEDDED KIVANVECHY SQAKVDGHTF SLGDFAYIKG EEEETHVGQI 

       610        620        630        640        650        660 
VEFFKTTDGE SYFRVQWFYR ATDTIMERQA TNHDKRRLFY STVMNDNPVD CLISKVTVLQ 

       670        680        690        700        710        720 
VSPRVGLKPN SIKSDYYFDM EYCVEYSTFQ TLRNPKTSEN KLECCADVVP TESTESILKK 

       730        740        750        760        770        780 
KSFSGELPVL DLYSGCGGMS TGLSLGAKIS GVDVVTKWAV DQNTAACKSL KLNHPNTQVR 

       790        800        810        820        830        840 
NDAAGDFLQL LKEWDKLCKR YVFNNDQRTD TLRSVNSTKE TSGSSSSSDD DSDSEEYEVE 

       850        860        870        880        890        900 
KLVDICFGDH DKTGKNGLKF KVHWKGYRSD EDTWELAEEL SNCQDAIREF VTSGFKSKIL 

       910        920        930        940        950        960 
PLPGRVGVIC GGPPCQGISG YNRHRNVDSP LNDERNQQII VFMDIVEYLK PSYVLMENVV 

       970        980        990       1000       1010       1020 
DILRMDKGSL GRYALSRLVN MRYQARLGIM TAGCYGLSQF RSRVFMWGAV PNKNLPPFPL 

      1030       1040       1050       1060       1070       1080 
PTHDVIVRYG LPLEFERNVV AYAEGQPRKL EKALVLKDAI SDLPHVSNDE DREKLPYESL 

      1090       1100       1110       1120       1130       1140 
PKTDFQRYIR STKRDLTGSA IDNCNKRTML LHDHRPFHIN EDDYARVCQI PKRKGANFRD 

      1150       1160       1170       1180       1190       1200 
LPGLIVRNNT VCRDPSMEPV ILPSGKPLVP GYVFTFQQGK SKRPFARLWW DETVPTVLTV 

      1210       1220       1230       1240       1250       1260 
PTCHSQALLH PEQDRVLTIR ESARLQGFPD YFQFCGTIKE RYCQIGNAVA VSVSRALGYS 

      1270       1280       1290 
LGMAFRGLAR DEHLIKLPQN FSHSTYPQLQ ETIPH 

« Hide

References

« Hide 'large scale' references
[1]"Arabidopsis cmt3 chromomethylase mutations block non-CG methylation and silencing of an endogenous gene."
Bartee L., Malagnac F., Bender J.
Genes Dev. 15:1753-1758(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: cv. Wassilewskija.
[2]"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. expand/collapse author list , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Whole genome sequence comparisons and 'full-length' cDNA sequences: a combined approach to evaluate and improve Arabidopsis genome annotation."
Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M., Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M., Weissenbach J., Salanoubat M.
Genome Res. 14:406-413(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1153-1295.
Strain: cv. Columbia.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF383171 Genomic DNA. Translation: AAK69757.1. Sequence problems.
AL021711 Genomic DNA. Translation: CAA16759.1. Sequence problems.
AL161549 Genomic DNA. Translation: CAB78904.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE84126.1.
BX828439 mRNA. No translation available.
PIRT05039.
RefSeqNP_193637.2. NM_118020.4.
UniGeneAt.32846.

3D structure databases

ProteinModelPortalQ94F87.
SMRQ94F87. Positions 523-1278.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid12933. 2 interactions.

Protein family/group databases

REBASE3168. M.AthCMT2.

Proteomic databases

PaxDbQ94F87.
PRIDEQ94F87.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT4G19020.1; AT4G19020.1; AT4G19020.
GeneID827640.
KEGGath:AT4G19020.

Organism-specific databases

TAIRAT4G19020.

Phylogenomic databases

eggNOGCOG0270.
InParanoidQ94F87.
KOK00558.
OMAGCQLRRS.
PhylomeDBQ94F87.

Enzyme and pathway databases

BioCycARA:AT4G19020-MONOMER.

Gene expression databases

GenevestigatorQ94F87.

Family and domain databases

InterProIPR001025. BAH_dom.
IPR001525. C5_MeTfrase.
IPR025821. C5_MeTfrase_pln.
IPR023780. Chromo_domain.
IPR000953. Chromo_domain/shadow.
IPR016197. Chromodomain-like.
[Graphical view]
PANTHERPTHR10629. PTHR10629. 1 hit.
PfamPF01426. BAH. 1 hit.
PF00385. Chromo. 1 hit.
PF00145. DNA_methylase. 1 hit.
[Graphical view]
PRINTSPR00105. C5METTRFRASE.
SMARTSM00439. BAH. 1 hit.
SM00298. CHROMO. 1 hit.
[Graphical view]
SUPFAMSSF54160. SSF54160. 1 hit.
PROSITEPS51038. BAH. 1 hit.
PS50013. CHROMO_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCMT2_ARATH
AccessionPrimary (citable) accession number: Q94F87
Secondary accession number(s): O49415
Entry history
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: February 8, 2011
Last modified: April 16, 2014
This is version 90 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names