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Q94F87

- CMT2_ARATH

UniProt

Q94F87 - CMT2_ARATH

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Protein

DNA (cytosine-5)-methyltransferase CMT2

Gene

CMT2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

May be involved in the CpXpG methylation and in gene silencing.By similarity

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei915 – 9151PROSITE-ProRule annotation

GO - Molecular functioni

  1. chromatin binding Source: InterPro
  2. DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC
  3. DNA binding Source: UniProtKB-KW

GO - Biological processi

  1. chromatin modification Source: UniProtKB-KW
  2. regulation of transcription, DNA-templated Source: UniProtKB-KW
  3. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Methyltransferase, Transferase

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciARA:AT4G19020-MONOMER.

Protein family/group databases

REBASEi3168. M.AthCMT2.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA (cytosine-5)-methyltransferase CMT2 (EC:2.1.1.37)
Alternative name(s):
Chromomethylase 2
Protein CHROMOMETHYLASE 2
Gene namesi
Name:CMT2
Ordered Locus Names:At4g19020
ORF Names:F13C5.190
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 4

Organism-specific databases

TAIRiAT4G19020.

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12951295DNA (cytosine-5)-methyltransferase CMT2PRO_0000246692Add
BLAST

Proteomic databases

PaxDbiQ94F87.
PRIDEiQ94F87.

Expressioni

Gene expression databases

GenevestigatoriQ94F87.

Interactioni

Protein-protein interaction databases

BioGridi12933. 3 interactions.
DIPiDIP-60718N.

Structurei

3D structure databases

ProteinModelPortaliQ94F87.
SMRiQ94F87. Positions 569-694, 720-778, 837-1263.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini578 – 693116BAHPROSITE-ProRule annotationAdd
BLAST
Domaini727 – 1268542SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd
BLAST
Domaini837 – 90266ChromoPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi822 – 8287Poly-Ser

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation
Contains 1 BAH domain.PROSITE-ProRule annotation
Contains 1 chromo domain.PROSITE-ProRule annotation
Contains 1 SAM-dependent MTase C5-type domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0270.
InParanoidiQ94F87.
KOiK00558.
OMAiGCQLRRS.
PhylomeDBiQ94F87.

Family and domain databases

Gene3Di3.40.50.150. 2 hits.
InterProiIPR001025. BAH_dom.
IPR001525. C5_MeTfrase.
IPR025821. C5_MeTfrase_pln.
IPR023780. Chromo_domain.
IPR000953. Chromo_domain/shadow.
IPR016197. Chromodomain-like.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PANTHERiPTHR10629. PTHR10629. 1 hit.
PfamiPF01426. BAH. 1 hit.
PF00385. Chromo. 1 hit.
PF00145. DNA_methylase. 1 hit.
[Graphical view]
PRINTSiPR00105. C5METTRFRASE.
SMARTiSM00439. BAH. 1 hit.
SM00298. CHROMO. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 4 hits.
SSF54160. SSF54160. 1 hit.
PROSITEiPS51038. BAH. 1 hit.
PS50013. CHROMO_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q94F87-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLSPAKCESE EAQAPLDLHS SSRSEPECLS LVLWCPNPEE AAPSSTRELI
60 70 80 90 100
KLPDNGEMSL RRSTTLNCNS PEENGGEGRV SQRKSSRGKS QPLLMLTNGC
110 120 130 140 150
QLRRSPRFRA LHANFDNVCS VPVTKGGVSQ RKFSRGKSQP LLTLTNGCQL
160 170 180 190 200
RRSPRFRAVD GNFDSVCSVP VTGKFGSRKR KSNSALDKKE SSDSEGLTFK
210 220 230 240 250
DIAVIAKSLE MEIISECQYK NNVAEGRSRL QDPAKRKVDS DTLLYSSINS
260 270 280 290 300
SKQSLGSNKR MRRSQRFMKG TENEGEENLG KSKGKGMSLA SCSFRRSTRL
310 320 330 340 350
SGTVETGNTE TLNRRKDCGP ALCGAEQVRG TERLVQISKK DHCCEAMKKC
360 370 380 390 400
EGDGLVSSKQ ELLVFPSGCI KKTVNGCRDR TLGKPRSSGL NTDDIHTSSL
410 420 430 440 450
KISKNDTSNG LTMTTALVEQ DAMESLLQGK TSACGAADKG KTREMHVNST
460 470 480 490 500
VIYLSDSDEP SSIEYLNGDN LTQVESGSAL SSGGNEGIVS LDLNNPTKST
510 520 530 540 550
KRKGKRVTRT AVQEQNKRSI CFFIGEPLSC EEAQERWRWR YELKERKSKS
560 570 580 590 600
RGQQSEDDED KIVANVECHY SQAKVDGHTF SLGDFAYIKG EEEETHVGQI
610 620 630 640 650
VEFFKTTDGE SYFRVQWFYR ATDTIMERQA TNHDKRRLFY STVMNDNPVD
660 670 680 690 700
CLISKVTVLQ VSPRVGLKPN SIKSDYYFDM EYCVEYSTFQ TLRNPKTSEN
710 720 730 740 750
KLECCADVVP TESTESILKK KSFSGELPVL DLYSGCGGMS TGLSLGAKIS
760 770 780 790 800
GVDVVTKWAV DQNTAACKSL KLNHPNTQVR NDAAGDFLQL LKEWDKLCKR
810 820 830 840 850
YVFNNDQRTD TLRSVNSTKE TSGSSSSSDD DSDSEEYEVE KLVDICFGDH
860 870 880 890 900
DKTGKNGLKF KVHWKGYRSD EDTWELAEEL SNCQDAIREF VTSGFKSKIL
910 920 930 940 950
PLPGRVGVIC GGPPCQGISG YNRHRNVDSP LNDERNQQII VFMDIVEYLK
960 970 980 990 1000
PSYVLMENVV DILRMDKGSL GRYALSRLVN MRYQARLGIM TAGCYGLSQF
1010 1020 1030 1040 1050
RSRVFMWGAV PNKNLPPFPL PTHDVIVRYG LPLEFERNVV AYAEGQPRKL
1060 1070 1080 1090 1100
EKALVLKDAI SDLPHVSNDE DREKLPYESL PKTDFQRYIR STKRDLTGSA
1110 1120 1130 1140 1150
IDNCNKRTML LHDHRPFHIN EDDYARVCQI PKRKGANFRD LPGLIVRNNT
1160 1170 1180 1190 1200
VCRDPSMEPV ILPSGKPLVP GYVFTFQQGK SKRPFARLWW DETVPTVLTV
1210 1220 1230 1240 1250
PTCHSQALLH PEQDRVLTIR ESARLQGFPD YFQFCGTIKE RYCQIGNAVA
1260 1270 1280 1290
VSVSRALGYS LGMAFRGLAR DEHLIKLPQN FSHSTYPQLQ ETIPH
Length:1,295
Mass (Da):145,015
Last modified:February 8, 2011 - v3
Checksum:i33CE317C541825A6
GO

Sequence cautioni

The sequence BX828439 differs from that shown. Reason: Erroneous termination at position 1226. Translated as Gln.
The sequence AAK69757.1 differs from that shown. Reason: Erroneous gene model prediction.
The sequence CAA16759.1 differs from that shown. Reason: Erroneous gene model prediction.
The sequence CAB78904.1 differs from that shown. Reason: Erroneous gene model prediction.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti54 – 552DN → ND in AAK69757. (PubMed:11459824)Curated
Sequence conflicti110 – 1112AL → PV in AAK69757. (PubMed:11459824)Curated
Sequence conflicti125 – 1251K → E in AAK69757. (PubMed:11459824)Curated
Sequence conflicti132 – 1332KF → NS in AAK69757. (PubMed:11459824)Curated
Sequence conflicti156 – 1561F → S in AAK69757. (PubMed:11459824)Curated
Sequence conflicti229 – 2291R → K in AAK69757. (PubMed:11459824)Curated
Sequence conflicti340 – 3401K → N in AAK69757. (PubMed:11459824)Curated
Sequence conflicti501 – 5011K → N in AAK69757. (PubMed:11459824)Curated
Sequence conflicti665 – 6651V → A in AAK69757. (PubMed:11459824)Curated
Sequence conflicti705 – 7051C → W in AAK69757. (PubMed:11459824)Curated
Sequence conflicti823 – 8231G → E in AAK69757. (PubMed:11459824)Curated
Sequence conflicti850 – 8501H → P in AAK69757. (PubMed:11459824)Curated
Sequence conflicti1132 – 11321K → N in AAK69757. (PubMed:11459824)Curated
Sequence conflicti1144 – 11441L → I in AAK69757. (PubMed:11459824)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF383171 Genomic DNA. Translation: AAK69757.1. Sequence problems.
AL021711 Genomic DNA. Translation: CAA16759.1. Sequence problems.
AL161549 Genomic DNA. Translation: CAB78904.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE84126.1.
BX828439 mRNA. No translation available.
PIRiT05039.
RefSeqiNP_193637.2. NM_118020.4.
UniGeneiAt.32846.

Genome annotation databases

EnsemblPlantsiAT4G19020.1; AT4G19020.1; AT4G19020.
GeneIDi827640.
KEGGiath:AT4G19020.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF383171 Genomic DNA. Translation: AAK69757.1 . Sequence problems.
AL021711 Genomic DNA. Translation: CAA16759.1 . Sequence problems.
AL161549 Genomic DNA. Translation: CAB78904.1 . Sequence problems.
CP002687 Genomic DNA. Translation: AEE84126.1 .
BX828439 mRNA. No translation available.
PIRi T05039.
RefSeqi NP_193637.2. NM_118020.4.
UniGenei At.32846.

3D structure databases

ProteinModelPortali Q94F87.
SMRi Q94F87. Positions 569-694, 720-778, 837-1263.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 12933. 3 interactions.
DIPi DIP-60718N.

Protein family/group databases

REBASEi 3168. M.AthCMT2.

Proteomic databases

PaxDbi Q94F87.
PRIDEi Q94F87.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT4G19020.1 ; AT4G19020.1 ; AT4G19020 .
GeneIDi 827640.
KEGGi ath:AT4G19020.

Organism-specific databases

TAIRi AT4G19020.

Phylogenomic databases

eggNOGi COG0270.
InParanoidi Q94F87.
KOi K00558.
OMAi GCQLRRS.
PhylomeDBi Q94F87.

Enzyme and pathway databases

BioCyci ARA:AT4G19020-MONOMER.

Gene expression databases

Genevestigatori Q94F87.

Family and domain databases

Gene3Di 3.40.50.150. 2 hits.
InterProi IPR001025. BAH_dom.
IPR001525. C5_MeTfrase.
IPR025821. C5_MeTfrase_pln.
IPR023780. Chromo_domain.
IPR000953. Chromo_domain/shadow.
IPR016197. Chromodomain-like.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
PANTHERi PTHR10629. PTHR10629. 1 hit.
Pfami PF01426. BAH. 1 hit.
PF00385. Chromo. 1 hit.
PF00145. DNA_methylase. 1 hit.
[Graphical view ]
PRINTSi PR00105. C5METTRFRASE.
SMARTi SM00439. BAH. 1 hit.
SM00298. CHROMO. 1 hit.
[Graphical view ]
SUPFAMi SSF53335. SSF53335. 4 hits.
SSF54160. SSF54160. 1 hit.
PROSITEi PS51038. BAH. 1 hit.
PS50013. CHROMO_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Arabidopsis cmt3 chromomethylase mutations block non-CG methylation and silencing of an endogenous gene."
    Bartee L., Malagnac F., Bender J.
    Genes Dev. 15:1753-1758(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. Wassilewskija.
  2. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
    Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
    , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
    Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Whole genome sequence comparisons and 'full-length' cDNA sequences: a combined approach to evaluate and improve Arabidopsis genome annotation."
    Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M., Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M., Weissenbach J., Salanoubat M.
    Genome Res. 14:406-413(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1153-1295.
    Strain: cv. Columbia.

Entry informationi

Entry nameiCMT2_ARATH
AccessioniPrimary (citable) accession number: Q94F87
Secondary accession number(s): O49415
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: February 8, 2011
Last modified: October 29, 2014
This is version 94 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3