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Q94F87

- CMT2_ARATH

UniProt

Q94F87 - CMT2_ARATH

Protein

DNA (cytosine-5)-methyltransferase CMT2

Gene

CMT2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 93 (01 Oct 2014)
      Sequence version 3 (08 Feb 2011)
      Previous versions | rss
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    Functioni

    May be involved in the CpXpG methylation and in gene silencing.By similarity

    Catalytic activityi

    S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei915 – 9151PROSITE-ProRule annotation

    GO - Molecular functioni

    1. chromatin binding Source: InterPro
    2. DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC
    3. DNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. chromatin modification Source: UniProtKB-KW
    2. regulation of transcription, DNA-templated Source: UniProtKB-KW
    3. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Chromatin regulator, Methyltransferase, Transferase

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciARA:AT4G19020-MONOMER.

    Protein family/group databases

    REBASEi3168. M.AthCMT2.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA (cytosine-5)-methyltransferase CMT2 (EC:2.1.1.37)
    Alternative name(s):
    Chromomethylase 2
    Protein CHROMOMETHYLASE 2
    Gene namesi
    Name:CMT2
    Ordered Locus Names:At4g19020
    ORF Names:F13C5.190
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 4

    Organism-specific databases

    TAIRiAT4G19020.

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 12951295DNA (cytosine-5)-methyltransferase CMT2PRO_0000246692Add
    BLAST

    Proteomic databases

    PaxDbiQ94F87.
    PRIDEiQ94F87.

    Expressioni

    Gene expression databases

    GenevestigatoriQ94F87.

    Interactioni

    Protein-protein interaction databases

    BioGridi12933. 3 interactions.
    DIPiDIP-60718N.

    Structurei

    3D structure databases

    ProteinModelPortaliQ94F87.
    SMRiQ94F87. Positions 523-1278.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini578 – 693116BAHPROSITE-ProRule annotationAdd
    BLAST
    Domaini727 – 1268542SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd
    BLAST
    Domaini837 – 90266ChromoPROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi822 – 8287Poly-Ser

    Sequence similaritiesi

    Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation
    Contains 1 BAH domain.PROSITE-ProRule annotation
    Contains 1 chromo domain.PROSITE-ProRule annotation
    Contains 1 SAM-dependent MTase C5-type domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0270.
    InParanoidiQ94F87.
    KOiK00558.
    OMAiGCQLRRS.
    PhylomeDBiQ94F87.

    Family and domain databases

    Gene3Di3.40.50.150. 2 hits.
    InterProiIPR001025. BAH_dom.
    IPR001525. C5_MeTfrase.
    IPR025821. C5_MeTfrase_pln.
    IPR023780. Chromo_domain.
    IPR000953. Chromo_domain/shadow.
    IPR016197. Chromodomain-like.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PANTHERiPTHR10629. PTHR10629. 1 hit.
    PfamiPF01426. BAH. 1 hit.
    PF00385. Chromo. 1 hit.
    PF00145. DNA_methylase. 1 hit.
    [Graphical view]
    PRINTSiPR00105. C5METTRFRASE.
    SMARTiSM00439. BAH. 1 hit.
    SM00298. CHROMO. 1 hit.
    [Graphical view]
    SUPFAMiSSF53335. SSF53335. 4 hits.
    SSF54160. SSF54160. 1 hit.
    PROSITEiPS51038. BAH. 1 hit.
    PS50013. CHROMO_2. 1 hit.
    PS51679. SAM_MT_C5. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q94F87-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLSPAKCESE EAQAPLDLHS SSRSEPECLS LVLWCPNPEE AAPSSTRELI     50
    KLPDNGEMSL RRSTTLNCNS PEENGGEGRV SQRKSSRGKS QPLLMLTNGC 100
    QLRRSPRFRA LHANFDNVCS VPVTKGGVSQ RKFSRGKSQP LLTLTNGCQL 150
    RRSPRFRAVD GNFDSVCSVP VTGKFGSRKR KSNSALDKKE SSDSEGLTFK 200
    DIAVIAKSLE MEIISECQYK NNVAEGRSRL QDPAKRKVDS DTLLYSSINS 250
    SKQSLGSNKR MRRSQRFMKG TENEGEENLG KSKGKGMSLA SCSFRRSTRL 300
    SGTVETGNTE TLNRRKDCGP ALCGAEQVRG TERLVQISKK DHCCEAMKKC 350
    EGDGLVSSKQ ELLVFPSGCI KKTVNGCRDR TLGKPRSSGL NTDDIHTSSL 400
    KISKNDTSNG LTMTTALVEQ DAMESLLQGK TSACGAADKG KTREMHVNST 450
    VIYLSDSDEP SSIEYLNGDN LTQVESGSAL SSGGNEGIVS LDLNNPTKST 500
    KRKGKRVTRT AVQEQNKRSI CFFIGEPLSC EEAQERWRWR YELKERKSKS 550
    RGQQSEDDED KIVANVECHY SQAKVDGHTF SLGDFAYIKG EEEETHVGQI 600
    VEFFKTTDGE SYFRVQWFYR ATDTIMERQA TNHDKRRLFY STVMNDNPVD 650
    CLISKVTVLQ VSPRVGLKPN SIKSDYYFDM EYCVEYSTFQ TLRNPKTSEN 700
    KLECCADVVP TESTESILKK KSFSGELPVL DLYSGCGGMS TGLSLGAKIS 750
    GVDVVTKWAV DQNTAACKSL KLNHPNTQVR NDAAGDFLQL LKEWDKLCKR 800
    YVFNNDQRTD TLRSVNSTKE TSGSSSSSDD DSDSEEYEVE KLVDICFGDH 850
    DKTGKNGLKF KVHWKGYRSD EDTWELAEEL SNCQDAIREF VTSGFKSKIL 900
    PLPGRVGVIC GGPPCQGISG YNRHRNVDSP LNDERNQQII VFMDIVEYLK 950
    PSYVLMENVV DILRMDKGSL GRYALSRLVN MRYQARLGIM TAGCYGLSQF 1000
    RSRVFMWGAV PNKNLPPFPL PTHDVIVRYG LPLEFERNVV AYAEGQPRKL 1050
    EKALVLKDAI SDLPHVSNDE DREKLPYESL PKTDFQRYIR STKRDLTGSA 1100
    IDNCNKRTML LHDHRPFHIN EDDYARVCQI PKRKGANFRD LPGLIVRNNT 1150
    VCRDPSMEPV ILPSGKPLVP GYVFTFQQGK SKRPFARLWW DETVPTVLTV 1200
    PTCHSQALLH PEQDRVLTIR ESARLQGFPD YFQFCGTIKE RYCQIGNAVA 1250
    VSVSRALGYS LGMAFRGLAR DEHLIKLPQN FSHSTYPQLQ ETIPH 1295
    Length:1,295
    Mass (Da):145,015
    Last modified:February 8, 2011 - v3
    Checksum:i33CE317C541825A6
    GO

    Sequence cautioni

    The sequence BX828439 differs from that shown. Reason: Erroneous termination at position 1226. Translated as Gln.
    The sequence AAK69757.1 differs from that shown. Reason: Erroneous gene model prediction.
    The sequence CAA16759.1 differs from that shown. Reason: Erroneous gene model prediction.
    The sequence CAB78904.1 differs from that shown. Reason: Erroneous gene model prediction.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti54 – 552DN → ND in AAK69757. (PubMed:11459824)Curated
    Sequence conflicti110 – 1112AL → PV in AAK69757. (PubMed:11459824)Curated
    Sequence conflicti125 – 1251K → E in AAK69757. (PubMed:11459824)Curated
    Sequence conflicti132 – 1332KF → NS in AAK69757. (PubMed:11459824)Curated
    Sequence conflicti156 – 1561F → S in AAK69757. (PubMed:11459824)Curated
    Sequence conflicti229 – 2291R → K in AAK69757. (PubMed:11459824)Curated
    Sequence conflicti340 – 3401K → N in AAK69757. (PubMed:11459824)Curated
    Sequence conflicti501 – 5011K → N in AAK69757. (PubMed:11459824)Curated
    Sequence conflicti665 – 6651V → A in AAK69757. (PubMed:11459824)Curated
    Sequence conflicti705 – 7051C → W in AAK69757. (PubMed:11459824)Curated
    Sequence conflicti823 – 8231G → E in AAK69757. (PubMed:11459824)Curated
    Sequence conflicti850 – 8501H → P in AAK69757. (PubMed:11459824)Curated
    Sequence conflicti1132 – 11321K → N in AAK69757. (PubMed:11459824)Curated
    Sequence conflicti1144 – 11441L → I in AAK69757. (PubMed:11459824)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF383171 Genomic DNA. Translation: AAK69757.1. Sequence problems.
    AL021711 Genomic DNA. Translation: CAA16759.1. Sequence problems.
    AL161549 Genomic DNA. Translation: CAB78904.1. Sequence problems.
    CP002687 Genomic DNA. Translation: AEE84126.1.
    BX828439 mRNA. No translation available.
    PIRiT05039.
    RefSeqiNP_193637.2. NM_118020.4.
    UniGeneiAt.32846.

    Genome annotation databases

    EnsemblPlantsiAT4G19020.1; AT4G19020.1; AT4G19020.
    GeneIDi827640.
    KEGGiath:AT4G19020.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF383171 Genomic DNA. Translation: AAK69757.1 . Sequence problems.
    AL021711 Genomic DNA. Translation: CAA16759.1 . Sequence problems.
    AL161549 Genomic DNA. Translation: CAB78904.1 . Sequence problems.
    CP002687 Genomic DNA. Translation: AEE84126.1 .
    BX828439 mRNA. No translation available.
    PIRi T05039.
    RefSeqi NP_193637.2. NM_118020.4.
    UniGenei At.32846.

    3D structure databases

    ProteinModelPortali Q94F87.
    SMRi Q94F87. Positions 523-1278.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 12933. 3 interactions.
    DIPi DIP-60718N.

    Protein family/group databases

    REBASEi 3168. M.AthCMT2.

    Proteomic databases

    PaxDbi Q94F87.
    PRIDEi Q94F87.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT4G19020.1 ; AT4G19020.1 ; AT4G19020 .
    GeneIDi 827640.
    KEGGi ath:AT4G19020.

    Organism-specific databases

    TAIRi AT4G19020.

    Phylogenomic databases

    eggNOGi COG0270.
    InParanoidi Q94F87.
    KOi K00558.
    OMAi GCQLRRS.
    PhylomeDBi Q94F87.

    Enzyme and pathway databases

    BioCyci ARA:AT4G19020-MONOMER.

    Gene expression databases

    Genevestigatori Q94F87.

    Family and domain databases

    Gene3Di 3.40.50.150. 2 hits.
    InterProi IPR001025. BAH_dom.
    IPR001525. C5_MeTfrase.
    IPR025821. C5_MeTfrase_pln.
    IPR023780. Chromo_domain.
    IPR000953. Chromo_domain/shadow.
    IPR016197. Chromodomain-like.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    PANTHERi PTHR10629. PTHR10629. 1 hit.
    Pfami PF01426. BAH. 1 hit.
    PF00385. Chromo. 1 hit.
    PF00145. DNA_methylase. 1 hit.
    [Graphical view ]
    PRINTSi PR00105. C5METTRFRASE.
    SMARTi SM00439. BAH. 1 hit.
    SM00298. CHROMO. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53335. SSF53335. 4 hits.
    SSF54160. SSF54160. 1 hit.
    PROSITEi PS51038. BAH. 1 hit.
    PS50013. CHROMO_2. 1 hit.
    PS51679. SAM_MT_C5. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Arabidopsis cmt3 chromomethylase mutations block non-CG methylation and silencing of an endogenous gene."
      Bartee L., Malagnac F., Bender J.
      Genes Dev. 15:1753-1758(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: cv. Wassilewskija.
    2. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
      Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
      , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
      Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Whole genome sequence comparisons and 'full-length' cDNA sequences: a combined approach to evaluate and improve Arabidopsis genome annotation."
      Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M., Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M., Weissenbach J., Salanoubat M.
      Genome Res. 14:406-413(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1153-1295.
      Strain: cv. Columbia.

    Entry informationi

    Entry nameiCMT2_ARATH
    AccessioniPrimary (citable) accession number: Q94F87
    Secondary accession number(s): O49415
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 25, 2006
    Last sequence update: February 8, 2011
    Last modified: October 1, 2014
    This is version 93 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3