Q94F87 (CMT2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA (cytosine-5)-methyltransferase CMT2
EC=2.1.1.37

Alternative name(s):
Chromomethylase 2
Protein CHROMOMETHYLASE 2

Gene names

Name:	CMT2
Ordered Locus Names:	At4g19020
ORF Names:	F13C5.190

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	1295 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May be involved in the CpXpG methylation and in gene silencing By similarity.
Catalytic activity	S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the C5-methyltransferase family. Contains 1 BAH domain. Contains 1 chromo domain.
Sequence caution	The sequence AAK69757.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence BX828439 differs from that shown. Reason: Erroneous termination at position 1226. Translated as Gln. The sequence CAA16759.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAB78904.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding S-adenosyl-L-methionine
Molecular function	Chromatin regulator Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	C-5 methylation of cytosine Inferred from electronic annotation. Source: GOC chromatin modification Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	DNA (cytosine-5-)-methyltransferase activity Inferred from electronic annotation. Source: EC DNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1295	1295	DNA (cytosine-5)-methyltransferase CMT2		PRO_0000246692
Regions
	Domain	578 – 693	116	BAH
	Domain	837 – 902	66	Chromo
	Compositional bias	822 – 828	7	Poly-Ser
Experimental info
	Sequence conflict	54 – 55	2	DN → ND in AAK69757. Ref.1
	Sequence conflict	110 – 111	2	AL → PV in AAK69757. Ref.1
	Sequence conflict	125	1	K → E in AAK69757. Ref.1
	Sequence conflict	132 – 133	2	KF → NS in AAK69757. Ref.1
	Sequence conflict	156	1	F → S in AAK69757. Ref.1
	Sequence conflict	229	1	R → K in AAK69757. Ref.1
	Sequence conflict	340	1	K → N in AAK69757. Ref.1
	Sequence conflict	501	1	K → N in AAK69757. Ref.1
	Sequence conflict	665	1	V → A in AAK69757. Ref.1
	Sequence conflict	705	1	C → W in AAK69757. Ref.1
	Sequence conflict	823	1	G → E in AAK69757. Ref.1
	Sequence conflict	850	1	H → P in AAK69757. Ref.1
	Sequence conflict	1132	1	K → N in AAK69757. Ref.1
	Sequence conflict	1144	1	L → I in AAK69757. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q94F87 [UniParc]. Last modified February 8, 2011. Version 3. Checksum: 33CE317C541825A6	FASTA	1,295	145,015
	10 20 30 40 50 60 MLSPAKCESE EAQAPLDLHS SSRSEPECLS LVLWCPNPEE AAPSSTRELI KLPDNGEMSL 70 80 90 100 110 120 RRSTTLNCNS PEENGGEGRV SQRKSSRGKS QPLLMLTNGC QLRRSPRFRA LHANFDNVCS 130 140 150 160 170 180 VPVTKGGVSQ RKFSRGKSQP LLTLTNGCQL RRSPRFRAVD GNFDSVCSVP VTGKFGSRKR 190 200 210 220 230 240 KSNSALDKKE SSDSEGLTFK DIAVIAKSLE MEIISECQYK NNVAEGRSRL QDPAKRKVDS 250 260 270 280 290 300 DTLLYSSINS SKQSLGSNKR MRRSQRFMKG TENEGEENLG KSKGKGMSLA SCSFRRSTRL 310 320 330 340 350 360 SGTVETGNTE TLNRRKDCGP ALCGAEQVRG TERLVQISKK DHCCEAMKKC EGDGLVSSKQ 370 380 390 400 410 420 ELLVFPSGCI KKTVNGCRDR TLGKPRSSGL NTDDIHTSSL KISKNDTSNG LTMTTALVEQ 430 440 450 460 470 480 DAMESLLQGK TSACGAADKG KTREMHVNST VIYLSDSDEP SSIEYLNGDN LTQVESGSAL 490 500 510 520 530 540 SSGGNEGIVS LDLNNPTKST KRKGKRVTRT AVQEQNKRSI CFFIGEPLSC EEAQERWRWR 550 560 570 580 590 600 YELKERKSKS RGQQSEDDED KIVANVECHY SQAKVDGHTF SLGDFAYIKG EEEETHVGQI 610 620 630 640 650 660 VEFFKTTDGE SYFRVQWFYR ATDTIMERQA TNHDKRRLFY STVMNDNPVD CLISKVTVLQ 670 680 690 700 710 720 VSPRVGLKPN SIKSDYYFDM EYCVEYSTFQ TLRNPKTSEN KLECCADVVP TESTESILKK 730 740 750 760 770 780 KSFSGELPVL DLYSGCGGMS TGLSLGAKIS GVDVVTKWAV DQNTAACKSL KLNHPNTQVR 790 800 810 820 830 840 NDAAGDFLQL LKEWDKLCKR YVFNNDQRTD TLRSVNSTKE TSGSSSSSDD DSDSEEYEVE 850 860 870 880 890 900 KLVDICFGDH DKTGKNGLKF KVHWKGYRSD EDTWELAEEL SNCQDAIREF VTSGFKSKIL 910 920 930 940 950 960 PLPGRVGVIC GGPPCQGISG YNRHRNVDSP LNDERNQQII VFMDIVEYLK PSYVLMENVV 970 980 990 1000 1010 1020 DILRMDKGSL GRYALSRLVN MRYQARLGIM TAGCYGLSQF RSRVFMWGAV PNKNLPPFPL 1030 1040 1050 1060 1070 1080 PTHDVIVRYG LPLEFERNVV AYAEGQPRKL EKALVLKDAI SDLPHVSNDE DREKLPYESL 1090 1100 1110 1120 1130 1140 PKTDFQRYIR STKRDLTGSA IDNCNKRTML LHDHRPFHIN EDDYARVCQI PKRKGANFRD 1150 1160 1170 1180 1190 1200 LPGLIVRNNT VCRDPSMEPV ILPSGKPLVP GYVFTFQQGK SKRPFARLWW DETVPTVLTV 1210 1220 1230 1240 1250 1260 PTCHSQALLH PEQDRVLTIR ESARLQGFPD YFQFCGTIKE RYCQIGNAVA VSVSRALGYS 1270 1280 1290 LGMAFRGLAR DEHLIKLPQN FSHSTYPQLQ ETIPH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Arabidopsis cmt3 chromomethylase mutations block non-CG methylation and silencing of an endogenous gene." Bartee L., Malagnac F., Bender J. Genes Dev. 15:1753-1758(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Wassilewskija.
[2]	"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana." Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R. Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Whole genome sequence comparisons and 'full-length' cDNA sequences: a combined approach to evaluate and improve Arabidopsis genome annotation." Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M., Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M., Weissenbach J., Salanoubat M. Genome Res. 14:406-413(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1153-1295. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF383171 Genomic DNA. Translation: AAK69757.1. Sequence problems. AL021711 Genomic DNA. Translation: CAA16759.1. Sequence problems. AL161549 Genomic DNA. Translation: CAB78904.1. Sequence problems. CP002687 Genomic DNA. Translation: AEE84126.1. BX828439 mRNA. No translation available.
IPI	IPI00539325.
PIR	T05039.
RefSeq	NP_193637.2. NM_118020.4.
UniGene	At.32846.
3D structure databases
ProteinModelPortal	Q94F87.
SMR	Q94F87. Positions 523-1278.
ModBase	Search...
Protein family/group databases
REBASE	3168. M.AthCMT2.
Proteomic databases
PaxDb	Q94F87.
PRIDE	Q94F87.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT4G19020.1; AT4G19020.1; AT4G19020.
GeneID	827640.
KEGG	ath:AT4G19020.
Organism-specific databases
TAIR	At4g19020.
Phylogenomic databases
eggNOG	COG0270.
InParanoid	Q94F87.
KO	K00558.
OMA	NGCQLRR.
PhylomeDB	Q94F87.
Gene expression databases
Genevestigator	Q94F87.
GermOnline	AT4G19020. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR001025. BAH_dom. IPR001525. C5_MeTfrase. IPR025821. C5_MeTfrase_pln. IPR023780. Chromo_domain. IPR000953. Chromo_domain/shadow. IPR016197. Chromodomain-like. [Graphical view]
PANTHER	PTHR10629. PTHR10629. 1 hit.
Pfam	PF01426. BAH. 1 hit. PF00385. Chromo. 1 hit. PF00145. DNA_methylase. 1 hit. [Graphical view]
PRINTS	PR00105. C5METTRFRASE.
SMART	SM00439. BAH. 1 hit. SM00298. CHROMO. 1 hit. [Graphical view]
SUPFAM	SSF54160. Chromodomain-like. 1 hit.
PROSITE	PS51038. BAH. 1 hit. PS00598. CHROMO_1. False negative. PS50013. CHROMO_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CMT2_ARATH

Accession

Primary (citable) accession number: Q94F87
Secondary accession number(s): O49415

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	February 8, 2011
Last modified:	May 1, 2013
This is version 83 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program