Reviewed, UniProtKB/Swiss-Prot Q94F87 (CMT2_ARATH)

Names and origin

Protein names

Recommended name:
    DNA (cytosine-5)-methyltransferase CMT2
    EC=2.1.1.37
Alternative name(s):
    Chromomethylase 2
    Protein CHROMOMETHYLASE 2

Gene names

Name:	CMT2
Ordered Locus Names:	At4g19020
ORF Names:	F13C5.190

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	1244 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	May be involved in the CpXpG methylation and in gene silencing By similarity.
Catalytic activity	S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the C5-methyltransferase family. Contains 1 BAH domain. Contains 1 chromo domain.
Sequence caution	The sequence CAA16759.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAB78904.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding S-adenosyl-L-methionine
Molecular function	Chromatin regulator Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA methylation Inferred from electronic annotation. Source: InterPro chromatin assembly or disassembly Inferred from electronic annotation. Source: InterPro chromatin modification Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chromatin Inferred from electronic annotation. Source: InterPro nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA (cytosine-5-)-methyltransferase activity Inferred from electronic annotation. Source: EC DNA binding Inferred from electronic annotation. Source: UniProtKB-KW chromatin binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1244	1244	DNA (cytosine-5)-methyltransferase CMT2		PRO_0000246692
Regions
	Domain	499 – 638	140	BAH
	Domain	786 – 851	66	Chromo
	Compositional bias	771 – 777	7	Poly-Ser
Experimental info
	Sequence conflict	54 – 55	2	DN → ND in AAK69757. Ref.1
	Sequence conflict	110 – 111	2	AL → PV in AAK69757. Ref.1
	Sequence conflict	125	1	K → E in AAK69757. Ref.1
	Sequence conflict	132 – 133	2	KF → NS in AAK69757. Ref.1
	Sequence conflict	156	1	F → S in AAK69757. Ref.1
	Sequence conflict	229	1	R → K in AAK69757. Ref.1
	Sequence conflict	340	1	K → N in AAK69757. Ref.1
	Sequence conflict	501	1	K → N in AAK69757. Ref.1
	Sequence conflict	548	1	A → V in AAK69757. Ref.1
	Sequence conflict	610	1	V → A in AAK69757. Ref.1
	Sequence conflict	654	1	C → W in AAK69757. Ref.1
	Sequence conflict	772	1	G → E in AAK69757. Ref.1
	Sequence conflict	799	1	H → P in AAK69757. Ref.1
	Sequence conflict	1081	1	K → N in AAK69757. Ref.1
	Sequence conflict	1093	1	L → I in AAK69757. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q94F87-1 [UniParc]. Last modified July 25, 2006. Version 2. Checksum: CBE10F5E47809340	FASTA	1,244	139,188
	10 20 30 40 50 60 MLSPAKCESE EAQAPLDLHS SSRSEPECLS LVLWCPNPEE AAPSSTRELI KLPDNGEMSL 70 80 90 100 110 120 RRSTTLNCNS PEENGGEGRV SQRKSSRGKS QPLLMLTNGC QLRRSPRFRA LHANFDNVCS 130 140 150 160 170 180 VPVTKGGVSQ RKFSRGKSQP LLTLTNGCQL RRSPRFRAVD GNFDSVCSVP VTGKFGSRKR 190 200 210 220 230 240 KSNSALDKKE SSDSEGLTFK DIAVIAKSLE MEIISECQYK NNVAEGRSRL QDPAKRKVDS 250 260 270 280 290 300 DTLLYSSINS SKQSLGSNKR MRRSQRFMKG TENEGEENLG KSKGKGMSLA SCSFRRSTRL 310 320 330 340 350 360 SGTVETGNTE TLNRRKDCGP ALCGAEQVRG TERLVQISKK DHCCEAMKKC EGDGLVSSKQ 370 380 390 400 410 420 ELLVFPSGCI KKTVNGCRDR TLGKPRSSGL NTDDIHTSSL KISKNDTSNG LTMTTALVEQ 430 440 450 460 470 480 DAMESLLQGK TSACGAADKG KTREMHVNST VIYLSDSDEP SSIEYLNGDN LTQVESGSAL 490 500 510 520 530 540 SSGGNEGIVS LDLNNPTKST KRKGKRVTRT AVQEQNKRSI CFFIGEPLSC EEAQERWRWR 550 560 570 580 590 600 YELKMEKATS EFSGFTEQRI QCVYKFFSFI MERQATNHDK RRLFYSTVMN DNPVDCLISK 610 620 630 640 650 660 VTVLQVSPRV GLKPNSIKSD YYFDMEYCVE YSTFQTLRNR KCSSKTSENK LECCADVVPT 670 680 690 700 710 720 ESTESILKKK SFSGELPVLD LYSGCGGMST GLSLGAKISG VDVVTKWAVD QNTAACKSLK 730 740 750 760 770 780 LNHPNTQVRN DAAGDFLQLL KEWDKLCKRY VFNNDQRTDT LRSVNSTKET SGSSSSSDDD 790 800 810 820 830 840 SDSEEYEVEK LVDICFGDHD KTGKNGLKFK VHWKGYRSDE DTWELAEELS NCQDAIREFV 850 860 870 880 890 900 TSGFKSKILP LPGRVGVICG GPPCQGISGY NRHRNVDSPL NDERNQQIIV FMDIVEYLKP 910 920 930 940 950 960 SYVLMENVVD ILRMDKGSLG RYALSRLVNM RYQARLGIMT AGCYGLSQFR SRVFMWGAVP 970 980 990 1000 1010 1020 NKNLPPFPLP THDVIVRYGL PLEFERNVVA YAEGQPRKLE KALVLKDAIS DLPHVSNDED 1030 1040 1050 1060 1070 1080 REKLPYESLP KTDFQRYIRS TKRDLTGSAI DNCNKRTMLL HDHRPFHINE DDYARVCQIP 1090 1100 1110 1120 1130 1140 KRKGANFRDL PGLIVRNNTV CRDPSMEPVI LPSGKPLVPG YVFTFQQGKS KRPFARLWWD 1150 1160 1170 1180 1190 1200 ETVPTVLTVP TCHSQALLHP EQDRVLTIRE SARLQGFPDY FQFCGTIKER YCQIGNAVAV 1210 1220 1230 1240 SVSRALGYSL GMAFRGLARD EHLIKLPQNF SHSTYPQLQE TIPH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Arabidopsis cmt3 chromomethylase mutations block non-CG methylation and silencing of an endogenous gene." Bartee L., Malagnac F., Bender J. Genes Dev. 15:1753-1758(2001) [PubMed: 11459824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Wassilewskija.
[2]	"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana." Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R. Nature 402:769-777(1999) [PubMed: 10617198] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF383171 Genomic DNA. Translation: AAK69757.1. AL021711 Genomic DNA. Translation: CAA16759.1. Sequence problems. AL161549 Genomic DNA. Translation: CAB78904.1. Sequence problems.
IPI	IPI00539325.
PIR	T05039.
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	Q94F87.
Genome annotation databases
GenomeReviews	Gene locus AT4G19020 in contig CT486007_GR.
Organism-specific databases
TAIR	At4g19020.
Phylogenomic databases
eggNOG	euNOG04911.
InParanoid	Q94F87.
PhylomeDB	Q94F87.
Enzyme and pathway databases
BRENDA	2.1.1.37. 302.
Gene expression databases
Genevestigator	Q94F87.
GermOnline	AT4G19020. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR001025. BAH_dom. IPR001525. C5_DNA_meth. IPR000953. Chromodomain. IPR016197. Chromodomain-like. [Graphical view]
PANTHER	PTHR10629. C5_DNA_meth. 1 hit.
Pfam	PF00385. Chromo. 1 hit. PF00145. DNA_methylase. 1 hit. [Graphical view]
PRINTS	PR00105. C5METTRFRASE.
SMART	SM00439. BAH. 1 hit. SM00298. CHROMO. 1 hit. [Graphical view]
PROSITE	PS51038. BAH. 1 hit. PS00598. CHROMO_1. False negative. PS50013. CHROMO_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CMT2_ARATH

Accession

Primary (citable) accession number: Q94F87
Secondary accession number(s): O49415

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	July 25, 2006
Last modified:	February 9, 2010
This is version 57 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)