Reviewed, UniProtKB/Swiss-Prot Q94F62 (BAK1_ARATH)

Names and origin

Protein names

Recommended name:
    BRASSINOSTEROID INSENSITIVE 1-associated receptor kinase 1
      Short name=BRI1-associated receptor kinase 1
    EC=2.7.11.1
Alternative name(s):
    Somatic embryogenesis receptor-like kinase 3

Gene names

Name:	BAK1
Synonyms:	SERK3
Ordered Locus Names:	At4g33430
ORF Names:	F17M5.190

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	615 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Serine/threonine-protein kinase involved in brassinosteroid signal transduction. Phosphorylates BRASSINOSTEROID INSENSITIVE 1 (BRI1). May be involved in changing the equilibrium between plasma membrane-located BRI1 homodimers and endocytosed BRI1-BAK1 heterodimers. Involved in pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI) via its interaction with PLS2.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subunit structure	Heterodimer with BRI1 in the endosomes. Interacts with FLS2. Ref.2 Ref.5 Ref.7 Ref.8
Subcellular location	Cell membrane; Single-pass type I membrane protein. Endosome membrane; Single-pass type I membrane protein. Ref.2 Ref.5 Ref.7
Tissue specificity	Expressed ubiquitously. Ref.2
Domain	Contains one leucine-zipper motif and one pair of conservatively spaced Cys (Cys pair) involved in forming heterodimers.
Miscellaneous	Interaction with BRI1 activates both receptor kinases and the full activation of either receptor kinase requires transphosphorylation by their partners. Autophosphorylates in vitro on Ser and Thr but not on Tyr.
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 4 LRR (leucine-rich) repeats. Contains 1 protein kinase domain.
Sequence caution	The sequence CAB38801.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAB80060.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Cell membrane Endosome Membrane
Domain	Leucine-rich repeat Repeat Signal Transmembrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	brassinosteroid mediated signaling Ref.5 Inferred from mutant phenotype. Source: TAIR cell death Inferred from mutant phenotype. Source: TAIR cell growth Inferred from mutant phenotype. Source: TAIR defense response to bacterium Inferred from mutant phenotype. Source: TAIR defense response to fungus Inferred from mutant phenotype. Source: TAIR defense response to oomycetes Inferred from mutant phenotype. Source: TAIR protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Cellular component	endosome membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Ref.5 Inferred from direct assay. Source: TAIR protein complex Inferred from physical interaction. Source: TAIR
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein heterodimerization activity Ref.7 Inferred from physical interaction. Source: TAIR protein serine/threonine kinase activity Ref.2 Inferred from direct assay. Source: TAIR
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
BRI1	O22476	4	EBI-617138,EBI-1797828
BRL1	Q9ZWC8	4	EBI-617138,EBI-590903
ERD13	P42761	2	EBI-617138,EBI-2265779
FLS2	Q9FL28	1	EBI-617138,EBI-1799448

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Potential
	Chain	26 – 615	590	BRASSINOSTEROID INSENSITIVE 1-associated receptor kinase 1		PRO_0000024304
Regions
	Transmembrane	226 – 246	21	Potential
	Repeat	91 – 115	25	LRR 1
	Repeat	117 – 139	23	LRR 2
	Repeat	140 – 163	24	LRR 3
	Repeat	165 – 188	24	LRR 4
	Domain	289 – 576	288	Protein kinase
	Nucleotide binding	295 – 303	9	ATP By similarity
	Motif	57 – 64	8	Cys pair
	Compositional bias	191 – 217	27	Pro-rich
Sites
	Active site	416	1	Proton acceptor By similarity
	Binding site	317	1	ATP By similarity
Amino acid modifications
	Modified residue	449	1	Phosphothreonine Ref.6
	Modified residue	450	1	Phosphothreonine Ref.6
	Glycosylation	79	1	N-linked (GlcNAc...) Potential
	Glycosylation	103	1	N-linked (GlcNAc...) Potential
	Glycosylation	114	1	N-linked (GlcNAc...) Potential
	Glycosylation	127	1	N-linked (GlcNAc...) Potential
	Glycosylation	149	1	N-linked (GlcNAc...) Potential
	Glycosylation	183	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	12	1	W → G in AAK68074. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q94F62-1 [UniParc]. Last modified March 28, 2003. Version 2. Checksum: 547B3F38FB46E1DD	FASTA	615	68,161
	10 20 30 40 50 60 MERRLMIPCF FWLILVLDLV LRVSGNAEGD ALSALKNSLA DPNKVLQSWD ATLVTPCTWF 70 80 90 100 110 120 HVTCNSDNSV TRVDLGNANL SGQLVMQLGQ LPNLQYLELY SNNITGTIPE QLGNLTELVS 130 140 150 160 170 180 LDLYLNNLSG PIPSTLGRLK KLRFLRLNNN SLSGEIPRSL TAVLTLQVLD LSNNPLTGDI 190 200 210 220 230 240 PVNGSFSLFT PISFANTKLT PLPASPPPPI SPTPPSPAGS NRITGAIAGG VAAGAALLFA 250 260 270 280 290 300 VPAIALAWWR RKKPQDHFFD VPAEEDPEVH LGQLKRFSLR ELQVASDNFS NKNILGRGGF 310 320 330 340 350 360 GKVYKGRLAD GTLVAVKRLK EERTQGGELQ FQTEVEMISM AVHRNLLRLR GFCMTPTERL 370 380 390 400 410 420 LVYPYMANGS VASCLRERPE SQPPLDWPKR QRIALGSARG LAYLHDHCDP KIIHRDVKAA 430 440 450 460 470 480 NILLDEEFEA VVGDFGLAKL MDYKDTHVTT AVRGTIGHIA PEYLSTGKSS EKTDVFGYGV 490 500 510 520 530 540 MLLELITGQR AFDLARLAND DDVMLLDWVK GLLKEKKLEA LVDVDLQGNY KDEEVEQLIQ 550 560 570 580 590 600 VALLCTQSSP MERPKMSEVV RMLEGDGLAE RWEEWQKEEM FRQDFNYPTH HPAVSGWIIG 610 DSTSQIENEY PSGPR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Arabidopsis SOMATIC EMBRYOGENESIS RECEPTOR KINASE 1 gene is expressed in developing ovules and embryos and enhances embryogenic competence in culture." Hecht V.F.G., Vielle-Calzada J.-P., Hartog M.V., Schmidt E.D.L., Boutilier K., Grossniklaus U., de Vries S.C. Plant Physiol. 127:803-816(2001) [PubMed: 11706164] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Landsberg erecta.
[2]	"BAK1, an Arabidopsis LRR receptor-like protein kinase, interacts with BRI1 and modulates brassinosteroid signaling." Li J., Wen J., Lease K.A., Doke J.T., Tax F.E., Walker J.C. Cell 110:213-222(2002) [PubMed: 12150929] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY, SUBCELLULAR LOCATION, PHOSPHORYLATION, INTERACTION WITH BRI1.
[3]	"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana." Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R. Nature 402:769-777(1999) [PubMed: 10617198] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	"Molecular characterisation of two novel maize LRR receptor-like kinases, which belong to the SERK gene family." Baudino S., Hansen S., Brettschneider R., Hecht V.F.G., Dresselhaus T., Loerz H., Dumas C., Rogowsky P.M. Planta 213:1-10(2001) [PubMed: 11523644] [Abstract] Cited for: CONCEPTUAL TRANSLATION.
[5]	"BRI1/BAK1, a receptor kinase pair mediating brassinosteroid signaling." Nam K.H., Li J. Cell 110:203-212(2002) [PubMed: 12150928] [Abstract] Cited for: SUBCELLULAR LOCATION, PHOSPHORYLATION, INTERACTION WITH BRI1.
[6]	"Phosphoproteomics of the Arabidopsis plasma membrane and a new phosphorylation site database." Nuehse T.S., Stensballe A., Jensen O.N., Peck S.C. Plant Cell 16:2394-2405(2004) [PubMed: 15308754] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-449 AND THR-450, MASS SPECTROMETRY.
[7]	"Heterodimerization and endocytosis of Arabidopsis brassinosteroid receptors BRI1 and AtSERK3 (BAK1)." Russinova E., Borst J.-W., Kwaaitaal M., Cano-Delgado A., Yin Y., Chory J., de Vries S.C. Plant Cell 16:3216-3229(2004) [PubMed: 15548744] [Abstract] Cited for: SUBCELLULAR LOCATION, INTERACTION WITH BRI1.
[8]	"The receptor-like kinase SERK3/BAK1 is a central regulator of innate immunity in plants." Heese A., Hann D.R., Gimenez-Ibanez S., Jones A.M.E., He K., Li J., Schroeder J.I., Peck S.C., Rathjen J.P. Proc. Natl. Acad. Sci. U.S.A. 104:12217-12222(2007) [PubMed: 17626179] [Abstract] Cited for: INTERACTION WITH FLS2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF384970 mRNA. Translation: AAK68074.1. AL035678 Genomic DNA. Translation: CAB38801.1. Sequence problems. AL161583 Genomic DNA. Translation: CAB80060.1. Sequence problems.
IPI	IPI00519519.
PIR	T05994.
RefSeq	NP_567920.1.
UniGene	At.376
3D structure databases
HSSP	HSSP built from PDB template 1I44 based on UniProtKB P06213.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q94F62. 6 interactions.
Proteomic databases
PRIDE	Q94F62.
Genome annotation databases
GeneID	829480.
GenomeReviews	Gene locus AT4G33430 in contig CT486007_GR.
KEGG	ath:AT4G33430.
NMPDR	fig|3702.1.peg.21385.
Organism-specific databases
GeneFarm	511. 46.
TAIR	At4g33430.
Phylogenomic databases
OMA	Q94F62. LKEERTQ.
Enzyme and pathway databases
BRENDA	2.7.11.1. 302.
Gene expression databases
ArrayExpress	Q94F62.
GermOnline	AT4G33430. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR001611. Leu-rich_rpt. IPR013210. Leu-rich_rpt_N. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. [Graphical view]
Pfam	PF00560. LRR_1. 4 hits. PF08263. LRRNT_2. 1 hit. PF00069. Pkinase. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BAK1_ARATH

Accession

Primary (citable) accession number: Q94F62
Secondary accession number(s): Q9SZC0

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 28, 2003
Last sequence update:	March 28, 2003
Last modified:	June 16, 2009
This is version 82 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)