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Q94F47

- UBC28_ARATH

UniProt

Q94F47 - UBC28_ARATH

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Protein

Ubiquitin-conjugating enzyme E2 28

Gene
UBC28, UBC9A, At1g64230, F22C12.2
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins.

Catalytic activityi

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei85 – 851Glycyl thioester intermediate By similarity

GO - Molecular functioni

  1. acid-amino acid ligase activity Source: InterPro
  2. ATP binding Source: UniProtKB-KW

GO - Biological processi

  1. protein ubiquitination Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT1G64230-MONOMER.
ARA:GQT-1299-MONOMER.
ARA:GQT-1300-MONOMER.
ARA:GQT-1301-MONOMER.
ARA:GQT-1302-MONOMER.
ReactomeiREACT_106068. Autodegradation of Cdh1 by Cdh1:APC/C.
REACT_185297. Separation of Sister Chromatids.
REACT_185305. APC/C:Cdc20 mediated degradation of Cyclin B.
REACT_187954. Senescence-Associated Secretory Phenotype (SASP).
REACT_208398. Inactivation of APC/C via direct inhibition of the APC/C complex.
REACT_94347. APC/C:Cdc20 mediated degradation of Securin.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 28 (EC:6.3.2.19)
Alternative name(s):
Ubiquitin carrier protein 28
Gene namesi
Name:UBC28
Synonyms:UBC9A
Ordered Locus Names:At1g64230
ORF Names:F22C12.2
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G64230.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 148148Ubiquitin-conjugating enzyme E2 28PRO_0000345193Add
BLAST

Proteomic databases

PaxDbiQ94F47.
PRIDEiQ94F47.

Expressioni

Tissue specificityi

Expressed in seeds, pistils, siliques, hypocotyls and leaves.1 Publication

Gene expression databases

GenevestigatoriQ94F47.

Interactioni

Protein-protein interaction databases

BioGridi27949. 7 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ94F47.
SMRiQ94F47. Positions 1-147.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG5078.
HOGENOMiHOG000233455.
InParanoidiQ94F47.
KOiK06689.
PhylomeDBiQ94F47.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. Align

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q94F47-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MASKRILKEL KDLQKDPPTS CSAGPVAEDM FHWQATIMGP SDSPYSGGVF    50
LVTIHFPPDY PFKPPKVAFR TKVFHPNVNS NGSICLDILK EQWSPALTIS 100
KVLLSICSLL TDPNPDDPLV PEIAHMYKTD RAKYESTARS WTQKYAMG 148
Length:148
Mass (Da):16,510
Last modified:December 1, 2001 - v1
Checksum:iD9F89BAF1801A83F
GO

Sequence cautioni

The sequence AAF24583.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF480945 mRNA. Translation: AAM11574.1.
DQ027041 mRNA. Translation: AAY44867.1.
AC007764 Genomic DNA. Translation: AAF24583.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE34212.1.
CP002684 Genomic DNA. Translation: AEE34213.1.
AF385718 mRNA. Translation: AAK60309.1.
AY133670 mRNA. Translation: AAM91500.1.
PIRiD96666.
RefSeqiNP_001031228.1. NM_001036151.1. [Q94F47-1]
NP_564828.1. NM_105097.8. [Q94F47-1]
UniGeneiAt.24180.

Genome annotation databases

EnsemblPlantsiAT1G64230.1; AT1G64230.1; AT1G64230. [Q94F47-1]
AT1G64230.2; AT1G64230.2; AT1G64230. [Q94F47-1]
GeneIDi842728.
KEGGiath:AT1G64230.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF480945 mRNA. Translation: AAM11574.1 .
DQ027041 mRNA. Translation: AAY44867.1 .
AC007764 Genomic DNA. Translation: AAF24583.1 . Sequence problems.
CP002684 Genomic DNA. Translation: AEE34212.1 .
CP002684 Genomic DNA. Translation: AEE34213.1 .
AF385718 mRNA. Translation: AAK60309.1 .
AY133670 mRNA. Translation: AAM91500.1 .
PIRi D96666.
RefSeqi NP_001031228.1. NM_001036151.1. [Q94F47-1 ]
NP_564828.1. NM_105097.8. [Q94F47-1 ]
UniGenei At.24180.

3D structure databases

ProteinModelPortali Q94F47.
SMRi Q94F47. Positions 1-147.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 27949. 7 interactions.

Proteomic databases

PaxDbi Q94F47.
PRIDEi Q94F47.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT1G64230.1 ; AT1G64230.1 ; AT1G64230 . [Q94F47-1 ]
AT1G64230.2 ; AT1G64230.2 ; AT1G64230 . [Q94F47-1 ]
GeneIDi 842728.
KEGGi ath:AT1G64230.

Organism-specific databases

TAIRi AT1G64230.

Phylogenomic databases

eggNOGi COG5078.
HOGENOMi HOG000233455.
InParanoidi Q94F47.
KOi K06689.
PhylomeDBi Q94F47.

Enzyme and pathway databases

UniPathwayi UPA00143 .
BioCyci ARA:AT1G64230-MONOMER.
ARA:GQT-1299-MONOMER.
ARA:GQT-1300-MONOMER.
ARA:GQT-1301-MONOMER.
ARA:GQT-1302-MONOMER.
Reactomei REACT_106068. Autodegradation of Cdh1 by Cdh1:APC/C.
REACT_185297. Separation of Sister Chromatids.
REACT_185305. APC/C:Cdc20 mediated degradation of Cyclin B.
REACT_187954. Senescence-Associated Secretory Phenotype (SASP).
REACT_208398. Inactivation of APC/C via direct inhibition of the APC/C complex.
REACT_94347. APC/C:Cdc20 mediated degradation of Securin.

Gene expression databases

Genevestigatori Q94F47.

Family and domain databases

Gene3Di 3.10.110.10. 1 hit.
InterProi IPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view ]
Pfami PF00179. UQ_con. 1 hit.
[Graphical view ]
SUPFAMi SSF54495. SSF54495. 1 hit.
PROSITEi PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "SINAT 5, a RING E3 ubiquitin protein ligase, promotes post-translational degradation of NAC 1 to attenuate auxin signals."
    Xie Q., Chua N.-H.
    Submitted (FEB-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Genome analysis and functional characterization of the E2 and RING-type E3 ligase ubiquitination enzymes of Arabidopsis."
    Kraft E., Stone S.L., Ma L., Su N., Gao Y., Lau O.-S., Deng X.-W., Callis J.
    Plant Physiol. 139:1597-1611(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, GENE FAMILY, NOMENCLATURE.
  3. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.

Entry informationi

Entry nameiUBC28_ARATH
AccessioniPrimary (citable) accession number: Q94F47
Secondary accession number(s): Q9SH72
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: December 1, 2001
Last modified: September 3, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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