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Q94F47

- UBC28_ARATH

UniProt

Q94F47 - UBC28_ARATH

Protein

Ubiquitin-conjugating enzyme E2 28

Gene

UBC28

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
  1. Functioni

    Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins.

    Catalytic activityi

    ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei85 – 851Glycyl thioester intermediatePROSITE-ProRule annotation

    GO - Molecular functioni

    1. acid-amino acid ligase activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW

    GO - Biological processi

    1. protein ubiquitination Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Ubl conjugation pathway

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT1G64230-MONOMER.
    ARA:GQT-1299-MONOMER.
    ARA:GQT-1300-MONOMER.
    ARA:GQT-1301-MONOMER.
    ARA:GQT-1302-MONOMER.
    ReactomeiREACT_106068. Autodegradation of Cdh1 by Cdh1:APC/C.
    REACT_185297. Separation of Sister Chromatids.
    REACT_185305. APC/C:Cdc20 mediated degradation of Cyclin B.
    REACT_187954. Senescence-Associated Secretory Phenotype (SASP).
    REACT_208398. Inactivation of APC/C via direct inhibition of the APC/C complex.
    REACT_94347. APC/C:Cdc20 mediated degradation of Securin.
    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin-conjugating enzyme E2 28 (EC:6.3.2.19)
    Alternative name(s):
    Ubiquitin carrier protein 28
    Gene namesi
    Name:UBC28
    Synonyms:UBC9A
    Ordered Locus Names:At1g64230
    ORF Names:F22C12.2
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G64230.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 148148Ubiquitin-conjugating enzyme E2 28PRO_0000345193Add
    BLAST

    Proteomic databases

    PaxDbiQ94F47.
    PRIDEiQ94F47.

    Expressioni

    Tissue specificityi

    Expressed in seeds, pistils, siliques, hypocotyls and leaves.1 Publication

    Gene expression databases

    GenevestigatoriQ94F47.

    Interactioni

    Protein-protein interaction databases

    BioGridi27949. 7 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ94F47.
    SMRiQ94F47. Positions 1-147.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5078.
    HOGENOMiHOG000233455.
    InParanoidiQ94F47.
    KOiK06689.
    PhylomeDBiQ94F47.

    Family and domain databases

    Gene3Di3.10.110.10. 1 hit.
    InterProiIPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view]
    PfamiPF00179. UQ_con. 1 hit.
    [Graphical view]
    SUPFAMiSSF54495. SSF54495. 1 hit.
    PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    This entry describes 1 isoform i produced by alternative splicing. Align

    Note: A number of isoforms are produced. According to EST sequences.

    Isoform 1 (identifier: Q94F47-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MASKRILKEL KDLQKDPPTS CSAGPVAEDM FHWQATIMGP SDSPYSGGVF    50
    LVTIHFPPDY PFKPPKVAFR TKVFHPNVNS NGSICLDILK EQWSPALTIS 100
    KVLLSICSLL TDPNPDDPLV PEIAHMYKTD RAKYESTARS WTQKYAMG 148
    Length:148
    Mass (Da):16,510
    Last modified:December 1, 2001 - v1
    Checksum:iD9F89BAF1801A83F
    GO

    Sequence cautioni

    The sequence AAF24583.1 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF480945 mRNA. Translation: AAM11574.1.
    DQ027041 mRNA. Translation: AAY44867.1.
    AC007764 Genomic DNA. Translation: AAF24583.1. Sequence problems.
    CP002684 Genomic DNA. Translation: AEE34212.1.
    CP002684 Genomic DNA. Translation: AEE34213.1.
    AF385718 mRNA. Translation: AAK60309.1.
    AY133670 mRNA. Translation: AAM91500.1.
    PIRiD96666.
    RefSeqiNP_001031228.1. NM_001036151.1. [Q94F47-1]
    NP_564828.1. NM_105097.8. [Q94F47-1]
    UniGeneiAt.24180.

    Genome annotation databases

    EnsemblPlantsiAT1G64230.1; AT1G64230.1; AT1G64230. [Q94F47-1]
    AT1G64230.2; AT1G64230.2; AT1G64230. [Q94F47-1]
    GeneIDi842728.
    KEGGiath:AT1G64230.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Web resourcesi

    PlantsUBQ

    A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF480945 mRNA. Translation: AAM11574.1 .
    DQ027041 mRNA. Translation: AAY44867.1 .
    AC007764 Genomic DNA. Translation: AAF24583.1 . Sequence problems.
    CP002684 Genomic DNA. Translation: AEE34212.1 .
    CP002684 Genomic DNA. Translation: AEE34213.1 .
    AF385718 mRNA. Translation: AAK60309.1 .
    AY133670 mRNA. Translation: AAM91500.1 .
    PIRi D96666.
    RefSeqi NP_001031228.1. NM_001036151.1. [Q94F47-1 ]
    NP_564828.1. NM_105097.8. [Q94F47-1 ]
    UniGenei At.24180.

    3D structure databases

    ProteinModelPortali Q94F47.
    SMRi Q94F47. Positions 1-147.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 27949. 7 interactions.

    Proteomic databases

    PaxDbi Q94F47.
    PRIDEi Q94F47.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G64230.1 ; AT1G64230.1 ; AT1G64230 . [Q94F47-1 ]
    AT1G64230.2 ; AT1G64230.2 ; AT1G64230 . [Q94F47-1 ]
    GeneIDi 842728.
    KEGGi ath:AT1G64230.

    Organism-specific databases

    TAIRi AT1G64230.

    Phylogenomic databases

    eggNOGi COG5078.
    HOGENOMi HOG000233455.
    InParanoidi Q94F47.
    KOi K06689.
    PhylomeDBi Q94F47.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .
    BioCyci ARA:AT1G64230-MONOMER.
    ARA:GQT-1299-MONOMER.
    ARA:GQT-1300-MONOMER.
    ARA:GQT-1301-MONOMER.
    ARA:GQT-1302-MONOMER.
    Reactomei REACT_106068. Autodegradation of Cdh1 by Cdh1:APC/C.
    REACT_185297. Separation of Sister Chromatids.
    REACT_185305. APC/C:Cdc20 mediated degradation of Cyclin B.
    REACT_187954. Senescence-Associated Secretory Phenotype (SASP).
    REACT_208398. Inactivation of APC/C via direct inhibition of the APC/C complex.
    REACT_94347. APC/C:Cdc20 mediated degradation of Securin.

    Gene expression databases

    Genevestigatori Q94F47.

    Family and domain databases

    Gene3Di 3.10.110.10. 1 hit.
    InterProi IPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view ]
    Pfami PF00179. UQ_con. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54495. SSF54495. 1 hit.
    PROSITEi PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "SINAT 5, a RING E3 ubiquitin protein ligase, promotes post-translational degradation of NAC 1 to attenuate auxin signals."
      Xie Q., Chua N.-H.
      Submitted (FEB-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Genome analysis and functional characterization of the E2 and RING-type E3 ligase ubiquitination enzymes of Arabidopsis."
      Kraft E., Stone S.L., Ma L., Su N., Gao Y., Lau O.-S., Deng X.-W., Callis J.
      Plant Physiol. 139:1597-1611(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, GENE FAMILY, NOMENCLATURE.
    3. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    4. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.

    Entry informationi

    Entry nameiUBC28_ARATH
    AccessioniPrimary (citable) accession number: Q94F47
    Secondary accession number(s): Q9SH72
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 22, 2008
    Last sequence update: December 1, 2001
    Last modified: October 1, 2014
    This is version 96 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3