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Protein

Ubiquitin-conjugating enzyme E2 28

Gene

UBC28

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins.

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei85Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-ATH-174184 Cdc20:Phospho-APC/C mediated degradation of Cyclin A
R-ATH-5689896 Ovarian tumor domain proteases
R-ATH-8866652 Synthesis of active ubiquitin: roles of E1 and E2 enzymes
R-ATH-8866654 E3 ubiquitin ligases ubiquitinate target proteins
R-ATH-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 28 (EC:2.3.2.23)
Alternative name(s):
AtUBC9A1 Publication
E2 ubiquitin-conjugating enzyme 28
Ubiquitin carrier protein 28
Gene namesi
Name:UBC281 Publication
Ordered Locus Names:At1g64230
ORF Names:F22C12.2
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G64230

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003451931 – 148Ubiquitin-conjugating enzyme E2 28Add BLAST148

Expressioni

Tissue specificityi

Expressed in seeds, pistils, siliques, hypocotyls and leaves.1 Publication

Gene expression databases

ExpressionAtlasiQ94F47 baseline and differential
GenevisibleiQ94F47 AT

Interactioni

Subunit structurei

Interacts with SINAT5.1 Publication

Protein-protein interaction databases

BioGridi27949, 8 interactors

Structurei

3D structure databases

ProteinModelPortaliQ94F47
SMRiQ94F47
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0417 Eukaryota
COG5078 LUCA
HOGENOMiHOG000233455
InParanoidiQ94F47
KOiK06689
PhylomeDBiQ94F47

Family and domain databases

CDDicd00195 UBCc, 1 hit
Gene3Di3.10.110.10, 1 hit
InterProiView protein in InterPro
IPR000608 UBQ-conjugat_E2
IPR023313 UBQ-conjugating_AS
IPR016135 UBQ-conjugating_enzyme/RWD
PfamiView protein in Pfam
PF00179 UQ_con, 1 hit
SUPFAMiSSF54495 SSF54495, 1 hit
PROSITEiView protein in PROSITE
PS00183 UBIQUITIN_CONJUGAT_1, 1 hit
PS50127 UBIQUITIN_CONJUGAT_2, 1 hit

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q94F47-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASKRILKEL KDLQKDPPTS CSAGPVAEDM FHWQATIMGP SDSPYSGGVF
60 70 80 90 100
LVTIHFPPDY PFKPPKVAFR TKVFHPNVNS NGSICLDILK EQWSPALTIS
110 120 130 140
KVLLSICSLL TDPNPDDPLV PEIAHMYKTD RAKYESTARS WTQKYAMG
Length:148
Mass (Da):16,510
Last modified:December 1, 2001 - v1
Checksum:iD9F89BAF1801A83F
GO

Sequence cautioni

The sequence AAF24583 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF480945 mRNA Translation: AAM11574.1
DQ027041 mRNA Translation: AAY44867.1
AC007764 Genomic DNA Translation: AAF24583.1 Sequence problems.
CP002684 Genomic DNA Translation: AEE34212.1
CP002684 Genomic DNA Translation: AEE34213.1
AF385718 mRNA Translation: AAK60309.1
AY133670 mRNA Translation: AAM91500.1
PIRiD96666
RefSeqiNP_001031228.1, NM_001036151.1 [Q94F47-1]
NP_564828.1, NM_105097.9 [Q94F47-1]
UniGeneiAt.24180

Genome annotation databases

EnsemblPlantsiAT1G64230.1; AT1G64230.1; AT1G64230 [Q94F47-1]
AT1G64230.2; AT1G64230.2; AT1G64230 [Q94F47-1]
GeneIDi842728
GrameneiAT1G64230.1; AT1G64230.1; AT1G64230 [Q94F47-1]
AT1G64230.2; AT1G64230.2; AT1G64230 [Q94F47-1]
KEGGiath:AT1G64230

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiUBC28_ARATH
AccessioniPrimary (citable) accession number: Q94F47
Secondary accession number(s): Q9SH72
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: December 1, 2001
Last modified: April 25, 2018
This is version 124 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health