Reviewed, UniProtKB/Swiss-Prot Q94EG3 (NEC1_NICLS)

Names and origin

Protein names

Recommended name:
    Nectarin-1
    EC=1.15.1.1
Alternative name(s):
    Superoxide dismutase [Mn]

Gene names

Name:

NECI

Organism

Nicotiana langsdorffii x Nicotiana sanderae (Ornamental tobacco)

Taxonomic identifier

164110 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Nicotianoideae › Nicotianeae › Nicotiana

Protein attributes

Sequence length	229 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May interact with bacterial adhesins thereby protecting the reproductive tissues from microbial attack. Has no oxalate oxidase activity. Ref.2
Catalytic activity	2 superoxide + 2 H+ = O2 + H2O2.
Cofactor	Binds 1 manganese ion per monomer. Ref.3
Subunit structure	Monomer. In the absence of manganese, it forms tetrameric and pentameric forms which show superoxide dismutase activity. Ref.3 Ref.2
Subcellular location	Secreted › extracellular space › apoplast. Note: Found in the nectar. Ref.2
Tissue specificity	Nectary tissues and to a lower level ovary. Not detected in petals, stems, leaves, roots or other floral tissues. Ref.2
Developmental stage	Expressed during nectar secretion, while the flowers remain open. Ref.2
Sequence similarities	Belongs to the germin family.
Mass spectrometry	Molecular mass is 22533±58 Da from positions 33 - 229. Determined by MALDI. Ref.3

Ontologies

Keywords
Cellular component	Apoplast Secreted
Domain	Signal
Ligand	Manganese Metal-binding
Molecular function	Oxidoreductase
PTM	Disulfide bond Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	apoplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW nutrient reservoir activity Inferred from electronic annotation. Source: InterPro superoxide dismutase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 32	32	Ref.2
	Chain	33 – 229	197	Nectarin-1 Ref.2		PRO_0000010840
Sites
	Metal binding	117	1	Manganese By similarity UniProtKB P45850
	Metal binding	119	1	Manganese By similarity UniProtKB P45850
	Metal binding	124	1	Manganese By similarity UniProtKB P45850
	Metal binding	163	1	Manganese By similarity UniProtKB P45850
Amino acid modifications
	Glycosylation	60	1	N-linked (GlcNAc...)
	Disulfide bond	42 ↔ 57		By similarity UniProtKB P45850
Experimental info
	Sequence conflict	59	1	T → A AA sequence Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q94EG3-1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: AEC7C89FD3E1AF63	FASTA	229	24,622
	10 20 30 40 50 60 MAAFGINSKI FQSMEMAILF LLAISIDRYC FAADEDMLQD VCVADLHSKV KVNGFPCKTN 70 80 90 100 110 120 FTAADFSSLA ISKPGATNNK FGSVVTTANV EQVPGLNTLG VSLARIDYAP GGINPPHTHP 130 140 150 160 170 180 RASEMVFVME GELDVGFITT ANVLVSKKII KGEVFVFPRG LVHFQKNNGE VPAAVISAFN 190 200 210 220 SQLPGTQSIP ITLFGASPPV PDDVLAQTFQ INTEDVQQIK SKFAPVKKF

References

[1]	"Cloning and characterization of a Nectarin I cDNA from Nicotiana langsdorffii x Nicotiana sanderae." Carter C., Thornburg R.W. Submitted (AUG-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Nectarin I is a novel, soluble germin-like protein expressed in the nectar of Nicotiana sp." Carter C., Graham R.A., Thornburg R.W. Plant Mol. Biol. 41:207-216(1999) [PubMed: 10579488] [Abstract] Cited for: PROTEIN SEQUENCE OF 33-77, SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[3]	"Tobacco nectarin I. Purification and characterization as a germin-like, manganese superoxide dismutase implicated in the defense of floral reproductive tissues." Carter C., Thornburg R.W. J. Biol. Chem. 275:36726-36733(2000) [PubMed: 10952990] [Abstract] Cited for: COFACTOR, SUBUNIT, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF411917 mRNA. Translation: AAK95664.1.
3D structure databases
SMR	Q94EG3. Positions 33-225.
ModBase	Search...
Family and domain databases
InterPro	IPR006045. Cupin_1. IPR011051. Cupin_RmlC_type. IPR001929. Germin. IPR019780. Germin_Mn-BS. IPR014710. RmlC-like_jellyroll. [Graphical view]
Gene3D	G3DSA:2.60.120.10. RmlC-like_jellyroll. 1 hit.
Pfam	PF00190. Cupin_1. 1 hit. [Graphical view]
PRINTS	PR00325. GERMIN.
SMART	SM00835. Cupin_1. 1 hit. [Graphical view]
PROSITE	PS00725. GERMIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NEC1_NICLS

Accession

Primary (citable) accession number: Q94EG3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 17, 2003
Last sequence update:	December 1, 2001
Last modified:	January 19, 2010
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)