Q94CG2 (BET12_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Bet1-like SNARE 1-2
Short name=AtBET12

Alternative name(s):
Bet1/Sft1-like SNARE 14b
Short name=AtBS14b

Gene names

Name:	BET12
Ordered Locus Names:	At4g14455
ORF Names:	FCAALL.379

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	130 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Required for vesicular transport from the ER to the Golgi complex. Functions as a SNARE associated with ER-derived vesicles By similarity.
Subcellular location	Golgi apparatus membrane; Single-pass type IV membrane protein By similarity. Endoplasmic reticulum membrane; Single-pass type IV membrane protein By similarity.
Sequence similarities	Belongs to the BET1 family. Contains 1 t-SNARE coiled-coil homology domain.
Sequence caution	The sequence CAB10224.1 differs from that shown. Reason: Erroneous gene model prediction. The predicted gene At4g14450 has been split into 2 genes: At4g14450 and At4g14455. The sequence CAB78487.1 differs from that shown. Reason: Erroneous gene model prediction. The predicted gene At4g14450 has been split into 2 genes: At4g14450 and At4g14455.

Ontologies

Keywords
Biological process	ER-Golgi transport Protein transport Transport
Cellular component	Endoplasmic reticulum Golgi apparatus Membrane
Domain	Coiled coil Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ER to Golgi vesicle-mediated transport Non-traceable author statement PubMed 11115874. Source: TAIR protein transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	SNAP receptor activity Inferred from genetic interaction Ref.5. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 130	130	Bet1-like SNARE 1-2		PRO_0000206888
Regions
	Topological domain	1 – 106	106	Cytoplasmic Potential
	Transmembrane	107 – 122	16	Helical; Anchor for type IV membrane protein; Potential
	Topological domain	123 – 130	8	Vesicular Potential
	Domain	33 – 95	63	t-SNARE coiled-coil homology
	Coiled coil	40 – 82	43	Potential
Experimental info
	Sequence conflict	65	1	E → K in AAK51151. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Q94CG2 [UniParc]. Last modified May 10, 2005. Version 4. Checksum: 64B12F2BCD11DCFF	FASTA	130	15,207
	10 20 30 40 50 60 MNFRRENRAS RTSLFDGLDG LEEGRLRASS SYAHDERDND EALENLQDRV SFLKRVTGDI 70 80 90 100 110 120 HEEVENHNRL LDKVGNKMDS ARGIMSGTIN RFKLVFEKKS NRKSCKLIAY FVLLFLIMYY 130 LIRLLNYIKG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of 1.9 Mb of contiguous sequence from chromosome 4 of Arabidopsis thaliana." Bevan M., Bancroft I., Bent E., Love K., Goodman H.M., Dean C., Bergkamp R., Dirkse W., van Staveren M., Stiekema W., Drost L., Ridley P., Hudson S.-A., Patel K., Murphy G., Piffanelli P., Wedler H., Wedler E. , Wambutt R., Weitzenegger T., Pohl T., Terryn N., Gielen J., Villarroel R., De Clercq R., van Montagu M., Lecharny A., Aubourg S., Gy I., Kreis M., Lao N., Kavanagh T., Hempel S., Kotter P., Entian K.-D., Rieger M., Schaefer M., Funk B., Mueller-Auer S., Silvey M., James R., Monfort A., Pons A., Puigdomenech P., Douka A., Voukelatou E., Milioni D., Hatzopoulos P., Piravandi E., Obermaier B., Hilbert H., Duesterhoeft A., Moores T., Jones J.D.G., Eneva T., Palme K., Benes V., Rechmann S., Ansorge W., Cooke R., Berger C., Delseny M., Voet M., Volckaert G., Mewes H.-W., Klosterman S., Schueller C., Chalwatzis N. Nature 391:485-488(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana." Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R. Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Functional annotation of a full-length Arabidopsis cDNA collection." Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T., Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y., Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K., Shinagawa A., Shinozaki K. Science 296:141-145(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"AtBS14a and AtBS14b, two Bet1/Sft1-like SNAREs from Arabidopsis thaliana that complement mutations in the yeast SFT1 gene." Tai W.C.S., Banfield D.K. FEBS Lett. 500:177-182(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 8-117.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z97336 Genomic DNA. Translation: CAB10224.1. Sequence problems. AL161539 Genomic DNA. Translation: CAB78487.1. Sequence problems. CP002687 Genomic DNA. Translation: AEE83447.1. AK117428 mRNA. Translation: BAC42093.1. AY033334 mRNA. Translation: AAK51151.1.
IPI	IPI00547668.
RefSeq	NP_849384.1. NM_179053.2.
UniGene	At.46134.
3D structure databases
ProteinModelPortal	Q94CG2.
SMR	Q94CG2. Positions 33-92.
ModBase	Search...
Proteomic databases
PaxDb	Q94CG2.
PRIDE	Q94CG2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT4G14455.1; AT4G14455.1; AT4G14455.
GeneID	827091.
KEGG	ath:AT4G14455.
Organism-specific databases
GeneFarm	4284. 431.
TAIR	At4g14455.
Phylogenomic databases
eggNOG	NOG129286.
HOGENOM	HOG000290697.
InParanoid	Q94CG2.
KO	K08504.
OMA	KLMCYII.
PhylomeDB	Q94CG2.
ProtClustDB	CLSN2684710.
Gene expression databases
Genevestigator	Q94CG2.
Family and domain databases
InterPro	IPR000727. T_SNARE_dom. [Graphical view]
Pfam	PF05739. SNARE. 1 hit. [Graphical view]
PROSITE	PS50192. T_SNARE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BET12_ARATH

Accession

Primary (citable) accession number: Q94CG2
Secondary accession number(s): O23301, Q8GYR9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 17, 2003
Last sequence update:	May 10, 2005
Last modified:	May 1, 2013
This is version 84 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program