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Q94CD8

- E134_ARATH

UniProt

Q94CD8 - E134_ARATH

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Protein

Glucan endo-1,3-beta-glucosidase 4

Gene
At3g13560, MRP15.22
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei266 – 2661Nucleophile By similarity
Active sitei329 – 3291Proton donor By similarity

GO - Molecular functioni

  1. glucan endo-1,3-beta-D-glucosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
  2. defense response Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Plant defense

Enzyme and pathway databases

BioCyciARA:AT3G13560-MONOMER.
ARA:GQT-719-MONOMER.
ARA:GQT-720-MONOMER.

Protein family/group databases

CAZyiCBM43. Carbohydrate-Binding Module Family 43.
GH17. Glycoside Hydrolase Family 17.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucan endo-1,3-beta-glucosidase 4 (EC:3.2.1.39)
Alternative name(s):
(1->3)-beta-glucan endohydrolase 4
Short name:
(1->3)-beta-glucanase 4
Beta-1,3-endoglucanase 4
Short name:
Beta-1,3-glucanase 4
Gene namesi
Ordered Locus Names:At3g13560
ORF Names:MRP15.22
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 3

Organism-specific databases

TAIRiAT3G13560.

Subcellular locationi

GO - Cellular componenti

  1. anchored component of membrane Source: TAIR
  2. anchored component of plasma membrane Source: TAIR
  3. plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323 Reviewed predictionAdd
BLAST
Chaini24 – 474451Glucan endo-1,3-beta-glucosidase 4PRO_0000011888Add
BLAST
Propeptidei475 – 50531Removed in mature form Reviewed predictionPRO_0000011889Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi70 – 701N-linked (GlcNAc...) Reviewed prediction
Glycosylationi110 – 1101N-linked (GlcNAc...) Reviewed prediction
Glycosylationi178 – 1781N-linked (GlcNAc...) Reviewed prediction
Glycosylationi256 – 2561N-linked (GlcNAc...) Reviewed prediction
Glycosylationi298 – 2981N-linked (GlcNAc...) Reviewed prediction
Glycosylationi338 – 3381N-linked (GlcNAc...) Reviewed prediction
Glycosylationi357 – 3571N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi363 ↔ 426 By similarity
Glycosylationi453 – 4531N-linked (GlcNAc...) Reviewed prediction
Lipidationi474 – 4741GPI-anchor amidated alanine Reviewed prediction

Post-translational modificationi

Contains two additional disulfide bonds By similarity.

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PaxDbiQ94CD8.
PRIDEiQ94CD8.

Expressioni

Gene expression databases

ArrayExpressiQ94CD8.
GenevestigatoriQ94CD8.

Structurei

3D structure databases

ProteinModelPortaliQ94CD8.
SMRiQ94CD8. Positions 26-347, 362-451.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG310730.
HOGENOMiHOG000238220.
InParanoidiQ94CD8.
OMAiQAMNFTG.
PhylomeDBiQ94CD8.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR000490. Glyco_hydro_17.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR012946. X8.
[Graphical view]
PfamiPF00332. Glyco_hydro_17. 1 hit.
PF07983. X8. 1 hit.
[Graphical view]
SMARTiSM00768. X8. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00587. GLYCOSYL_HYDROL_F17. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q94CD8-1 [UniParc]FASTAAdd to Basket

« Hide

MLLPRWFAEA LLLLLSILAC SNAAFIGVNI GTDLTNMPPP SDIVTLLKSQ    50
QITHVRLYDA NSHMLKAFAN TSIEVMVGVT NEEILKIGRF PSAAAAWVNK 100
NVAAYIPSTN ITAIAVGSEV LTTIPHVAPI LASALNNIHK ALVASNLNFK 150
VKVSSPMSMD IMPKPFPPST STFSPSWNTT VYQLLQFLKN TGSFFMLNAY 200
PYYGYTTANG IFPLDYALFK QLSPVKQIVD PNTLLHYNSM FDAMVDAAYY 250
SMEALNFSKI PVVVTETGWP SSGGSDEAAA TVANAETFNT NLIKRVLNNS 300
GPPSQPDIPI NTYIYELYNE DKRSGPVSER NWGILFPNGT SVYPLSLSGG 350
SSSAALNGSS MFCVAKADAD DDKLVDGLNW ACGQGRANCA AIQPGQPCYL 400
PNDVKSHASF AFNDYYQKMK SAGGTCDFDG TAITTTRDPS YRTCAYTGSL 450
NANATNGNFP PDALGPASPL GGNANARIIF SYHLPILAPL ALTLLQLLLQ 500
HDRLL 505
Length:505
Mass (Da):54,416
Last modified:December 1, 2001 - v1
Checksum:i4C6CA8CA9952B671
GO

Sequence cautioni

The sequence BAB01763.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP000603 Genomic DNA. Translation: BAB01763.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE75372.1.
CP002686 Genomic DNA. Translation: AEE75373.1.
CP002686 Genomic DNA. Translation: AEE75374.1.
AY034940 mRNA. Translation: AAK59446.1.
AY063117 mRNA. Translation: AAL34291.1.
RefSeqiNP_187965.1. NM_112202.4.
NP_974302.1. NM_202573.2.
NP_974303.1. NM_202574.2.
UniGeneiAt.8269.

Genome annotation databases

EnsemblPlantsiAT3G13560.1; AT3G13560.1; AT3G13560.
AT3G13560.2; AT3G13560.2; AT3G13560.
AT3G13560.3; AT3G13560.3; AT3G13560.
GeneIDi820558.
KEGGiath:AT3G13560.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP000603 Genomic DNA. Translation: BAB01763.1 . Sequence problems.
CP002686 Genomic DNA. Translation: AEE75372.1 .
CP002686 Genomic DNA. Translation: AEE75373.1 .
CP002686 Genomic DNA. Translation: AEE75374.1 .
AY034940 mRNA. Translation: AAK59446.1 .
AY063117 mRNA. Translation: AAL34291.1 .
RefSeqi NP_187965.1. NM_112202.4.
NP_974302.1. NM_202573.2.
NP_974303.1. NM_202574.2.
UniGenei At.8269.

3D structure databases

ProteinModelPortali Q94CD8.
SMRi Q94CD8. Positions 26-347, 362-451.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi CBM43. Carbohydrate-Binding Module Family 43.
GH17. Glycoside Hydrolase Family 17.

Proteomic databases

PaxDbi Q94CD8.
PRIDEi Q94CD8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT3G13560.1 ; AT3G13560.1 ; AT3G13560 .
AT3G13560.2 ; AT3G13560.2 ; AT3G13560 .
AT3G13560.3 ; AT3G13560.3 ; AT3G13560 .
GeneIDi 820558.
KEGGi ath:AT3G13560.

Organism-specific databases

TAIRi AT3G13560.

Phylogenomic databases

eggNOGi NOG310730.
HOGENOMi HOG000238220.
InParanoidi Q94CD8.
OMAi QAMNFTG.
PhylomeDBi Q94CD8.

Enzyme and pathway databases

BioCyci ARA:AT3G13560-MONOMER.
ARA:GQT-719-MONOMER.
ARA:GQT-720-MONOMER.

Gene expression databases

ArrayExpressi Q94CD8.
Genevestigatori Q94CD8.

Family and domain databases

Gene3Di 3.20.20.80. 1 hit.
InterProi IPR000490. Glyco_hydro_17.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR012946. X8.
[Graphical view ]
Pfami PF00332. Glyco_hydro_17. 1 hit.
PF07983. X8. 1 hit.
[Graphical view ]
SMARTi SM00768. X8. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 1 hit.
PROSITEi PS00587. GLYCOSYL_HYDROL_F17. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones."
    Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.
    DNA Res. 7:217-221(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Identification of glycosylphosphatidylinositol-anchored proteins in Arabidopsis. A proteomic and genomic analysis."
    Borner G.H.H., Lilley K.S., Stevens T.J., Dupree P.
    Plant Physiol. 132:568-577(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: GPI-ANCHOR [LARGE SCALE ANALYSIS].
    Strain: cv. Columbia.
    Tissue: Callus.
  5. "Proteomic analysis of glycosylphosphatidylinositol-anchored membrane proteins."
    Elortza F., Nuehse T.S., Foster L.J., Stensballe A., Peck S.C., Jensen O.N.
    Mol. Cell. Proteomics 2:1261-1270(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: GPI-ANCHOR [LARGE SCALE ANALYSIS].
  6. "Modification-specific proteomics of plasma membrane proteins: identification and characterization of glycosylphosphatidylinositol-anchored proteins released upon phospholipase D treatment."
    Elortza F., Mohammed S., Bunkenborg J., Foster L.J., Nuehse T.S., Brodbeck U., Peck S.C., Jensen O.N.
    J. Proteome Res. 5:935-943(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: GPI-ANCHOR [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiE134_ARATH
AccessioniPrimary (citable) accession number: Q94CD8
Secondary accession number(s): Q9LJD6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2003
Last sequence update: December 1, 2001
Last modified: September 3, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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