Q94CD8 (E134_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glucan endo-1,3-beta-glucosidase 4
EC=3.2.1.39

Alternative name(s):
(1->3)-beta-glucan endohydrolase 4
Short name=(1->3)-beta-glucanase 4
Beta-1,3-endoglucanase 4
Short name=Beta-1,3-glucanase 4

Gene names

Ordered Locus Names:	At3g13560
ORF Names:	MRP15.22

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	505 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.
Subcellular location	Cell membrane; Lipid-anchor › GPI-anchor.
Sequence similarities	Belongs to the glycosyl hydrolase 17 family.
Sequence caution	The sequence BAB01763.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Plant defense
Cellular component	Cell membrane Membrane
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	GPI-anchor Glycoprotein Lipoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro defense response Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	anchored to plasma membrane Inferred from direct assay Ref.4. Source: TAIR
Molecular_function	glucan endo-1,3-beta-D-glucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 474	451	Glucan endo-1,3-beta-glucosidase 4		PRO_0000011888
	Propeptide	475 – 505	31	Removed in mature form Potential		PRO_0000011889
Sites
	Active site	266	1	Nucleophile By similarity
	Active site	329	1	Proton donor By similarity
Amino acid modifications
	Lipidation	474	1	GPI-anchor amidated alanine Potential
	Glycosylation	70	1	N-linked (GlcNAc...) Potential
	Glycosylation	110	1	N-linked (GlcNAc...) Potential
	Glycosylation	178	1	N-linked (GlcNAc...) Potential
	Glycosylation	256	1	N-linked (GlcNAc...) Potential
	Glycosylation	298	1	N-linked (GlcNAc...) Potential
	Glycosylation	338	1	N-linked (GlcNAc...) Potential
	Glycosylation	357	1	N-linked (GlcNAc...) Potential
	Glycosylation	453	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q94CD8 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 4C6CA8CA9952B671	FASTA	505	54,416
	10 20 30 40 50 60 MLLPRWFAEA LLLLLSILAC SNAAFIGVNI GTDLTNMPPP SDIVTLLKSQ QITHVRLYDA 70 80 90 100 110 120 NSHMLKAFAN TSIEVMVGVT NEEILKIGRF PSAAAAWVNK NVAAYIPSTN ITAIAVGSEV 130 140 150 160 170 180 LTTIPHVAPI LASALNNIHK ALVASNLNFK VKVSSPMSMD IMPKPFPPST STFSPSWNTT 190 200 210 220 230 240 VYQLLQFLKN TGSFFMLNAY PYYGYTTANG IFPLDYALFK QLSPVKQIVD PNTLLHYNSM 250 260 270 280 290 300 FDAMVDAAYY SMEALNFSKI PVVVTETGWP SSGGSDEAAA TVANAETFNT NLIKRVLNNS 310 320 330 340 350 360 GPPSQPDIPI NTYIYELYNE DKRSGPVSER NWGILFPNGT SVYPLSLSGG SSSAALNGSS 370 380 390 400 410 420 MFCVAKADAD DDKLVDGLNW ACGQGRANCA AIQPGQPCYL PNDVKSHASF AFNDYYQKMK 430 440 450 460 470 480 SAGGTCDFDG TAITTTRDPS YRTCAYTGSL NANATNGNFP PDALGPASPL GGNANARIIF 490 500 SYHLPILAPL ALTLLQLLLQ HDRLL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones." Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S. DNA Res. 7:217-221(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Identification of glycosylphosphatidylinositol-anchored proteins in Arabidopsis. A proteomic and genomic analysis." Borner G.H.H., Lilley K.S., Stevens T.J., Dupree P. Plant Physiol. 132:568-577(2003) [PubMed] [Europe PMC] [Abstract] Cited for: GPI-ANCHOR [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. Strain: cv. Columbia. Tissue: Callus.
[5]	"Proteomic analysis of glycosylphosphatidylinositol-anchored membrane proteins." Elortza F., Nuehse T.S., Foster L.J., Stensballe A., Peck S.C., Jensen O.N. Mol. Cell. Proteomics 2:1261-1270(2003) [PubMed] [Europe PMC] [Abstract] Cited for: GPI-ANCHOR [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[6]	"Modification-specific proteomics of plasma membrane proteins: identification and characterization of glycosylphosphatidylinositol-anchored proteins released upon phospholipase D treatment." Elortza F., Mohammed S., Bunkenborg J., Foster L.J., Nuehse T.S., Brodbeck U., Peck S.C., Jensen O.N. J. Proteome Res. 5:935-943(2006) [PubMed] [Europe PMC] [Abstract] Cited for: GPI-ANCHOR [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP000603 Genomic DNA. Translation: BAB01763.1. Sequence problems. CP002686 Genomic DNA. Translation: AEE75372.1. CP002686 Genomic DNA. Translation: AEE75373.1. CP002686 Genomic DNA. Translation: AEE75374.1. AY034940 mRNA. Translation: AAK59446.1. AY063117 mRNA. Translation: AAL34291.1.
IPI	IPI00537250.
RefSeq	NP_187965.1. NM_112202.4. NP_974302.1. NM_202573.2. NP_974303.1. NM_202574.2.
UniGene	At.8269.
3D structure databases
ProteinModelPortal	Q94CD8.
SMR	Q94CD8. Positions 26-347, 362-451.
ModBase	Search...
Protein family/group databases
CAZy	CBM43. Carbohydrate-Binding Module Family 43. GH17. Glycoside Hydrolase Family 17.
Proteomic databases
PaxDb	Q94CD8.
PRIDE	Q94CD8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT3G13560.1; AT3G13560.1; AT3G13560. AT3G13560.2; AT3G13560.2; AT3G13560. AT3G13560.3; AT3G13560.3; AT3G13560.
GeneID	820558.
KEGG	ath:AT3G13560.
Organism-specific databases
TAIR	At3g13560.
Phylogenomic databases
eggNOG	NOG310730.
HOGENOM	HOG000238220.
InParanoid	Q94CD8.
OMA	KPFPPST.
PhylomeDB	Q94CD8.
ProtClustDB	CLSN2684782.
Gene expression databases
ArrayExpress	Q94CD8.
Genevestigator	Q94CD8.
GermOnline	AT3G13560. Arabidopsis thaliana.
Family and domain databases
Gene3D	3.20.20.80. 1 hit.
InterPro	IPR000490. Glyco_hydro_17. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. IPR012946. X8. [Graphical view]
Pfam	PF00332. Glyco_hydro_17. 1 hit. PF07983. X8. 1 hit. [Graphical view]
SMART	SM00768. X8. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00587. GLYCOSYL_HYDROL_F17. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

E134_ARATH

Accession

Primary (citable) accession number: Q94CD8
Secondary accession number(s): Q9LJD6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2003
Last sequence update:	December 1, 2001
Last modified:	May 1, 2013
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program