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Protein

Glucan endo-1,3-beta-glucosidase 4

Gene

At3g13560

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei266NucleophileBy similarity1
Active sitei329Proton donorBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Plant defense

Enzyme and pathway databases

BioCyciARA:AT3G13560-MONOMER.

Protein family/group databases

CAZyiCBM43. Carbohydrate-Binding Module Family 43.
GH17. Glycoside Hydrolase Family 17.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucan endo-1,3-beta-glucosidase 4 (EC:3.2.1.39)
Alternative name(s):
(1->3)-beta-glucan endohydrolase 4
Short name:
(1->3)-beta-glucanase 4
Beta-1,3-endoglucanase 4
Short name:
Beta-1,3-glucanase 4
Gene namesi
Ordered Locus Names:At3g13560
ORF Names:MRP15.22
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G13560.

Subcellular locationi

GO - Cellular componenti

  • anchored component of membrane Source: TAIR
  • anchored component of plasma membrane Source: TAIR
  • plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
ChainiPRO_000001188824 – 474Glucan endo-1,3-beta-glucosidase 4Add BLAST451
PropeptideiPRO_0000011889475 – 505Removed in mature formSequence analysisAdd BLAST31

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi70N-linked (GlcNAc...)Sequence analysis1
Glycosylationi110N-linked (GlcNAc...)Sequence analysis1
Glycosylationi178N-linked (GlcNAc...)Sequence analysis1
Glycosylationi256N-linked (GlcNAc...)Sequence analysis1
Glycosylationi298N-linked (GlcNAc...)Sequence analysis1
Glycosylationi338N-linked (GlcNAc...)Sequence analysis1
Glycosylationi357N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi363 ↔ 426By similarity
Glycosylationi453N-linked (GlcNAc...)Sequence analysis1
Lipidationi474GPI-anchor amidated alanineSequence analysis1

Post-translational modificationi

Contains two additional disulfide bonds.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PaxDbiQ94CD8.
PRIDEiQ94CD8.

Expressioni

Gene expression databases

ExpressionAtlasiQ94CD8. baseline and differential.
GenevisibleiQ94CD8. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT3G13560.1.

Structurei

3D structure databases

ProteinModelPortaliQ94CD8.
SMRiQ94CD8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 17 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IF5R. Eukaryota.
ENOG410YASF. LUCA.
HOGENOMiHOG000238220.
InParanoidiQ94CD8.
KOiK19892.
OMAiPYYGYTT.
OrthoDBiEOG0936058Z.
PhylomeDBiQ94CD8.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR000490. Glyco_hydro_17.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR012946. X8.
[Graphical view]
PfamiPF00332. Glyco_hydro_17. 1 hit.
PF07983. X8. 1 hit.
[Graphical view]
SMARTiSM00768. X8. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00587. GLYCOSYL_HYDROL_F17. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q94CD8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLPRWFAEA LLLLLSILAC SNAAFIGVNI GTDLTNMPPP SDIVTLLKSQ
60 70 80 90 100
QITHVRLYDA NSHMLKAFAN TSIEVMVGVT NEEILKIGRF PSAAAAWVNK
110 120 130 140 150
NVAAYIPSTN ITAIAVGSEV LTTIPHVAPI LASALNNIHK ALVASNLNFK
160 170 180 190 200
VKVSSPMSMD IMPKPFPPST STFSPSWNTT VYQLLQFLKN TGSFFMLNAY
210 220 230 240 250
PYYGYTTANG IFPLDYALFK QLSPVKQIVD PNTLLHYNSM FDAMVDAAYY
260 270 280 290 300
SMEALNFSKI PVVVTETGWP SSGGSDEAAA TVANAETFNT NLIKRVLNNS
310 320 330 340 350
GPPSQPDIPI NTYIYELYNE DKRSGPVSER NWGILFPNGT SVYPLSLSGG
360 370 380 390 400
SSSAALNGSS MFCVAKADAD DDKLVDGLNW ACGQGRANCA AIQPGQPCYL
410 420 430 440 450
PNDVKSHASF AFNDYYQKMK SAGGTCDFDG TAITTTRDPS YRTCAYTGSL
460 470 480 490 500
NANATNGNFP PDALGPASPL GGNANARIIF SYHLPILAPL ALTLLQLLLQ

HDRLL
Length:505
Mass (Da):54,416
Last modified:December 1, 2001 - v1
Checksum:i4C6CA8CA9952B671
GO

Sequence cautioni

The sequence BAB01763 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP000603 Genomic DNA. Translation: BAB01763.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE75372.1.
CP002686 Genomic DNA. Translation: AEE75373.1.
CP002686 Genomic DNA. Translation: AEE75374.1.
AY034940 mRNA. Translation: AAK59446.1.
AY063117 mRNA. Translation: AAL34291.1.
RefSeqiNP_187965.1. NM_112202.5.
NP_974302.1. NM_202573.3.
NP_974303.1. NM_202574.3.
UniGeneiAt.8269.

Genome annotation databases

EnsemblPlantsiAT3G13560.1; AT3G13560.1; AT3G13560.
AT3G13560.2; AT3G13560.2; AT3G13560.
AT3G13560.3; AT3G13560.3; AT3G13560.
GeneIDi820558.
GrameneiAT3G13560.1; AT3G13560.1; AT3G13560.
AT3G13560.2; AT3G13560.2; AT3G13560.
AT3G13560.3; AT3G13560.3; AT3G13560.
KEGGiath:AT3G13560.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP000603 Genomic DNA. Translation: BAB01763.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE75372.1.
CP002686 Genomic DNA. Translation: AEE75373.1.
CP002686 Genomic DNA. Translation: AEE75374.1.
AY034940 mRNA. Translation: AAK59446.1.
AY063117 mRNA. Translation: AAL34291.1.
RefSeqiNP_187965.1. NM_112202.5.
NP_974302.1. NM_202573.3.
NP_974303.1. NM_202574.3.
UniGeneiAt.8269.

3D structure databases

ProteinModelPortaliQ94CD8.
SMRiQ94CD8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT3G13560.1.

Protein family/group databases

CAZyiCBM43. Carbohydrate-Binding Module Family 43.
GH17. Glycoside Hydrolase Family 17.

Proteomic databases

PaxDbiQ94CD8.
PRIDEiQ94CD8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G13560.1; AT3G13560.1; AT3G13560.
AT3G13560.2; AT3G13560.2; AT3G13560.
AT3G13560.3; AT3G13560.3; AT3G13560.
GeneIDi820558.
GrameneiAT3G13560.1; AT3G13560.1; AT3G13560.
AT3G13560.2; AT3G13560.2; AT3G13560.
AT3G13560.3; AT3G13560.3; AT3G13560.
KEGGiath:AT3G13560.

Organism-specific databases

TAIRiAT3G13560.

Phylogenomic databases

eggNOGiENOG410IF5R. Eukaryota.
ENOG410YASF. LUCA.
HOGENOMiHOG000238220.
InParanoidiQ94CD8.
KOiK19892.
OMAiPYYGYTT.
OrthoDBiEOG0936058Z.
PhylomeDBiQ94CD8.

Enzyme and pathway databases

BioCyciARA:AT3G13560-MONOMER.

Miscellaneous databases

PROiQ94CD8.

Gene expression databases

ExpressionAtlasiQ94CD8. baseline and differential.
GenevisibleiQ94CD8. AT.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR000490. Glyco_hydro_17.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR012946. X8.
[Graphical view]
PfamiPF00332. Glyco_hydro_17. 1 hit.
PF07983. X8. 1 hit.
[Graphical view]
SMARTiSM00768. X8. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00587. GLYCOSYL_HYDROL_F17. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiE134_ARATH
AccessioniPrimary (citable) accession number: Q94CD8
Secondary accession number(s): Q9LJD6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2003
Last sequence update: December 1, 2001
Last modified: November 30, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.