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Q94CD8 (E134_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glucan endo-1,3-beta-glucosidase 4

EC=3.2.1.39
Alternative name(s):
(1->3)-beta-glucan endohydrolase 4
Short name=(1->3)-beta-glucanase 4
Beta-1,3-endoglucanase 4
Short name=Beta-1,3-glucanase 4
Gene names
Ordered Locus Names:At3g13560
ORF Names:MRP15.22
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length505 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.

Subcellular location

Cell membrane; Lipid-anchorGPI-anchor.

Sequence similarities

Belongs to the glycosyl hydrolase 17 family.

Sequence caution

The sequence BAB01763.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 Potential
Chain24 – 474451Glucan endo-1,3-beta-glucosidase 4
PRO_0000011888
Propeptide475 – 50531Removed in mature form Potential
PRO_0000011889

Sites

Active site2661Nucleophile By similarity
Active site3291Proton donor By similarity

Amino acid modifications

Lipidation4741GPI-anchor amidated alanine Potential
Glycosylation701N-linked (GlcNAc...) Potential
Glycosylation1101N-linked (GlcNAc...) Potential
Glycosylation1781N-linked (GlcNAc...) Potential
Glycosylation2561N-linked (GlcNAc...) Potential
Glycosylation2981N-linked (GlcNAc...) Potential
Glycosylation3381N-linked (GlcNAc...) Potential
Glycosylation3571N-linked (GlcNAc...) Potential
Glycosylation4531N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q94CD8 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: 4C6CA8CA9952B671

FASTA50554,416
        10         20         30         40         50         60 
MLLPRWFAEA LLLLLSILAC SNAAFIGVNI GTDLTNMPPP SDIVTLLKSQ QITHVRLYDA 

        70         80         90        100        110        120 
NSHMLKAFAN TSIEVMVGVT NEEILKIGRF PSAAAAWVNK NVAAYIPSTN ITAIAVGSEV 

       130        140        150        160        170        180 
LTTIPHVAPI LASALNNIHK ALVASNLNFK VKVSSPMSMD IMPKPFPPST STFSPSWNTT 

       190        200        210        220        230        240 
VYQLLQFLKN TGSFFMLNAY PYYGYTTANG IFPLDYALFK QLSPVKQIVD PNTLLHYNSM 

       250        260        270        280        290        300 
FDAMVDAAYY SMEALNFSKI PVVVTETGWP SSGGSDEAAA TVANAETFNT NLIKRVLNNS 

       310        320        330        340        350        360 
GPPSQPDIPI NTYIYELYNE DKRSGPVSER NWGILFPNGT SVYPLSLSGG SSSAALNGSS 

       370        380        390        400        410        420 
MFCVAKADAD DDKLVDGLNW ACGQGRANCA AIQPGQPCYL PNDVKSHASF AFNDYYQKMK 

       430        440        450        460        470        480 
SAGGTCDFDG TAITTTRDPS YRTCAYTGSL NANATNGNFP PDALGPASPL GGNANARIIF 

       490        500 
SYHLPILAPL ALTLLQLLLQ HDRLL 

« Hide

References

« Hide 'large scale' references
[1]"Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones."
Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.
DNA Res. 7:217-221(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"Identification of glycosylphosphatidylinositol-anchored proteins in Arabidopsis. A proteomic and genomic analysis."
Borner G.H.H., Lilley K.S., Stevens T.J., Dupree P.
Plant Physiol. 132:568-577(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: GPI-ANCHOR [LARGE SCALE ANALYSIS].
Strain: cv. Columbia.
Tissue: Callus.
[5]"Proteomic analysis of glycosylphosphatidylinositol-anchored membrane proteins."
Elortza F., Nuehse T.S., Foster L.J., Stensballe A., Peck S.C., Jensen O.N.
Mol. Cell. Proteomics 2:1261-1270(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: GPI-ANCHOR [LARGE SCALE ANALYSIS].
[6]"Modification-specific proteomics of plasma membrane proteins: identification and characterization of glycosylphosphatidylinositol-anchored proteins released upon phospholipase D treatment."
Elortza F., Mohammed S., Bunkenborg J., Foster L.J., Nuehse T.S., Brodbeck U., Peck S.C., Jensen O.N.
J. Proteome Res. 5:935-943(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: GPI-ANCHOR [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP000603 Genomic DNA. Translation: BAB01763.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE75372.1.
CP002686 Genomic DNA. Translation: AEE75373.1.
CP002686 Genomic DNA. Translation: AEE75374.1.
AY034940 mRNA. Translation: AAK59446.1.
AY063117 mRNA. Translation: AAL34291.1.
RefSeqNP_187965.1. NM_112202.4.
NP_974302.1. NM_202573.2.
NP_974303.1. NM_202574.2.
UniGeneAt.8269.

3D structure databases

ProteinModelPortalQ94CD8.
SMRQ94CD8. Positions 26-347, 362-451.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyCBM43. Carbohydrate-Binding Module Family 43.
GH17. Glycoside Hydrolase Family 17.

Proteomic databases

PaxDbQ94CD8.
PRIDEQ94CD8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT3G13560.1; AT3G13560.1; AT3G13560.
AT3G13560.2; AT3G13560.2; AT3G13560.
AT3G13560.3; AT3G13560.3; AT3G13560.
GeneID820558.
KEGGath:AT3G13560.

Organism-specific databases

TAIRAT3G13560.

Phylogenomic databases

eggNOGNOG310730.
HOGENOMHOG000238220.
InParanoidQ94CD8.
OMAQAMNFTG.
PhylomeDBQ94CD8.

Enzyme and pathway databases

BioCycARA:AT3G13560-MONOMER.
ARA:GQT-719-MONOMER.
ARA:GQT-720-MONOMER.

Gene expression databases

ArrayExpressQ94CD8.
GenevestigatorQ94CD8.

Family and domain databases

Gene3D3.20.20.80. 1 hit.
InterProIPR000490. Glyco_hydro_17.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR012946. X8.
[Graphical view]
PfamPF00332. Glyco_hydro_17. 1 hit.
PF07983. X8. 1 hit.
[Graphical view]
SMARTSM00768. X8. 1 hit.
[Graphical view]
SUPFAMSSF51445. SSF51445. 1 hit.
PROSITEPS00587. GLYCOSYL_HYDROL_F17. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameE134_ARATH
AccessionPrimary (citable) accession number: Q94CD8
Secondary accession number(s): Q9LJD6
Entry history
Integrated into UniProtKB/Swiss-Prot: October 31, 2003
Last sequence update: December 1, 2001
Last modified: May 14, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names