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Q94CD8

- E134_ARATH

UniProt

Q94CD8 - E134_ARATH

Protein

Glucan endo-1,3-beta-glucosidase 4

Gene

At3g13560

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 99 (01 Oct 2014)
      Sequence version 1 (01 Dec 2001)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei266 – 2661NucleophileBy similarity
    Active sitei329 – 3291Proton donorBy similarity

    GO - Molecular functioni

    1. glucan endo-1,3-beta-D-glucosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro
    2. defense response Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Plant defense

    Enzyme and pathway databases

    BioCyciARA:AT3G13560-MONOMER.
    ARA:GQT-719-MONOMER.
    ARA:GQT-720-MONOMER.

    Protein family/group databases

    CAZyiCBM43. Carbohydrate-Binding Module Family 43.
    GH17. Glycoside Hydrolase Family 17.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glucan endo-1,3-beta-glucosidase 4 (EC:3.2.1.39)
    Alternative name(s):
    (1->3)-beta-glucan endohydrolase 4
    Short name:
    (1->3)-beta-glucanase 4
    Beta-1,3-endoglucanase 4
    Short name:
    Beta-1,3-glucanase 4
    Gene namesi
    Ordered Locus Names:At3g13560
    ORF Names:MRP15.22
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 3

    Organism-specific databases

    TAIRiAT3G13560.

    Subcellular locationi

    GO - Cellular componenti

    1. anchored component of membrane Source: TAIR
    2. anchored component of plasma membrane Source: TAIR
    3. plasma membrane Source: TAIR

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2323Sequence AnalysisAdd
    BLAST
    Chaini24 – 474451Glucan endo-1,3-beta-glucosidase 4PRO_0000011888Add
    BLAST
    Propeptidei475 – 50531Removed in mature formSequence AnalysisPRO_0000011889Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi70 – 701N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi110 – 1101N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi178 – 1781N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi256 – 2561N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi298 – 2981N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi338 – 3381N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi357 – 3571N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi363 ↔ 426By similarity
    Glycosylationi453 – 4531N-linked (GlcNAc...)Sequence Analysis
    Lipidationi474 – 4741GPI-anchor amidated alanineSequence Analysis

    Post-translational modificationi

    Contains two additional disulfide bonds.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

    Proteomic databases

    PaxDbiQ94CD8.
    PRIDEiQ94CD8.

    Expressioni

    Gene expression databases

    ArrayExpressiQ94CD8.
    GenevestigatoriQ94CD8.

    Structurei

    3D structure databases

    ProteinModelPortaliQ94CD8.
    SMRiQ94CD8. Positions 26-347, 362-451.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 17 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG310730.
    HOGENOMiHOG000238220.
    InParanoidiQ94CD8.
    OMAiQAMNFTG.
    PhylomeDBiQ94CD8.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR000490. Glyco_hydro_17.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR012946. X8.
    [Graphical view]
    PfamiPF00332. Glyco_hydro_17. 1 hit.
    PF07983. X8. 1 hit.
    [Graphical view]
    SMARTiSM00768. X8. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.
    PROSITEiPS00587. GLYCOSYL_HYDROL_F17. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q94CD8-1 [UniParc]FASTAAdd to Basket

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    MLLPRWFAEA LLLLLSILAC SNAAFIGVNI GTDLTNMPPP SDIVTLLKSQ    50
    QITHVRLYDA NSHMLKAFAN TSIEVMVGVT NEEILKIGRF PSAAAAWVNK 100
    NVAAYIPSTN ITAIAVGSEV LTTIPHVAPI LASALNNIHK ALVASNLNFK 150
    VKVSSPMSMD IMPKPFPPST STFSPSWNTT VYQLLQFLKN TGSFFMLNAY 200
    PYYGYTTANG IFPLDYALFK QLSPVKQIVD PNTLLHYNSM FDAMVDAAYY 250
    SMEALNFSKI PVVVTETGWP SSGGSDEAAA TVANAETFNT NLIKRVLNNS 300
    GPPSQPDIPI NTYIYELYNE DKRSGPVSER NWGILFPNGT SVYPLSLSGG 350
    SSSAALNGSS MFCVAKADAD DDKLVDGLNW ACGQGRANCA AIQPGQPCYL 400
    PNDVKSHASF AFNDYYQKMK SAGGTCDFDG TAITTTRDPS YRTCAYTGSL 450
    NANATNGNFP PDALGPASPL GGNANARIIF SYHLPILAPL ALTLLQLLLQ 500
    HDRLL 505
    Length:505
    Mass (Da):54,416
    Last modified:December 1, 2001 - v1
    Checksum:i4C6CA8CA9952B671
    GO

    Sequence cautioni

    The sequence BAB01763.1 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP000603 Genomic DNA. Translation: BAB01763.1. Sequence problems.
    CP002686 Genomic DNA. Translation: AEE75372.1.
    CP002686 Genomic DNA. Translation: AEE75373.1.
    CP002686 Genomic DNA. Translation: AEE75374.1.
    AY034940 mRNA. Translation: AAK59446.1.
    AY063117 mRNA. Translation: AAL34291.1.
    RefSeqiNP_187965.1. NM_112202.4.
    NP_974302.1. NM_202573.2.
    NP_974303.1. NM_202574.2.
    UniGeneiAt.8269.

    Genome annotation databases

    EnsemblPlantsiAT3G13560.1; AT3G13560.1; AT3G13560.
    AT3G13560.2; AT3G13560.2; AT3G13560.
    AT3G13560.3; AT3G13560.3; AT3G13560.
    GeneIDi820558.
    KEGGiath:AT3G13560.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP000603 Genomic DNA. Translation: BAB01763.1 . Sequence problems.
    CP002686 Genomic DNA. Translation: AEE75372.1 .
    CP002686 Genomic DNA. Translation: AEE75373.1 .
    CP002686 Genomic DNA. Translation: AEE75374.1 .
    AY034940 mRNA. Translation: AAK59446.1 .
    AY063117 mRNA. Translation: AAL34291.1 .
    RefSeqi NP_187965.1. NM_112202.4.
    NP_974302.1. NM_202573.2.
    NP_974303.1. NM_202574.2.
    UniGenei At.8269.

    3D structure databases

    ProteinModelPortali Q94CD8.
    SMRi Q94CD8. Positions 26-347, 362-451.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi CBM43. Carbohydrate-Binding Module Family 43.
    GH17. Glycoside Hydrolase Family 17.

    Proteomic databases

    PaxDbi Q94CD8.
    PRIDEi Q94CD8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT3G13560.1 ; AT3G13560.1 ; AT3G13560 .
    AT3G13560.2 ; AT3G13560.2 ; AT3G13560 .
    AT3G13560.3 ; AT3G13560.3 ; AT3G13560 .
    GeneIDi 820558.
    KEGGi ath:AT3G13560.

    Organism-specific databases

    TAIRi AT3G13560.

    Phylogenomic databases

    eggNOGi NOG310730.
    HOGENOMi HOG000238220.
    InParanoidi Q94CD8.
    OMAi QAMNFTG.
    PhylomeDBi Q94CD8.

    Enzyme and pathway databases

    BioCyci ARA:AT3G13560-MONOMER.
    ARA:GQT-719-MONOMER.
    ARA:GQT-720-MONOMER.

    Gene expression databases

    ArrayExpressi Q94CD8.
    Genevestigatori Q94CD8.

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    InterProi IPR000490. Glyco_hydro_17.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR012946. X8.
    [Graphical view ]
    Pfami PF00332. Glyco_hydro_17. 1 hit.
    PF07983. X8. 1 hit.
    [Graphical view ]
    SMARTi SM00768. X8. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 1 hit.
    PROSITEi PS00587. GLYCOSYL_HYDROL_F17. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones."
      Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.
      DNA Res. 7:217-221(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "Identification of glycosylphosphatidylinositol-anchored proteins in Arabidopsis. A proteomic and genomic analysis."
      Borner G.H.H., Lilley K.S., Stevens T.J., Dupree P.
      Plant Physiol. 132:568-577(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: GPI-ANCHOR [LARGE SCALE ANALYSIS].
      Strain: cv. Columbia.
      Tissue: Callus.
    5. "Proteomic analysis of glycosylphosphatidylinositol-anchored membrane proteins."
      Elortza F., Nuehse T.S., Foster L.J., Stensballe A., Peck S.C., Jensen O.N.
      Mol. Cell. Proteomics 2:1261-1270(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: GPI-ANCHOR [LARGE SCALE ANALYSIS].
    6. "Modification-specific proteomics of plasma membrane proteins: identification and characterization of glycosylphosphatidylinositol-anchored proteins released upon phospholipase D treatment."
      Elortza F., Mohammed S., Bunkenborg J., Foster L.J., Nuehse T.S., Brodbeck U., Peck S.C., Jensen O.N.
      J. Proteome Res. 5:935-943(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: GPI-ANCHOR [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiE134_ARATH
    AccessioniPrimary (citable) accession number: Q94CD8
    Secondary accession number(s): Q9LJD6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 31, 2003
    Last sequence update: December 1, 2001
    Last modified: October 1, 2014
    This is version 99 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3