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Q94CD5 (ATG7_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin-like modifier-activating enzyme atg7
Alternative name(s):
ATG12-activating enzyme E1 atg7
Autophagy-related protein 7
Short name=AtAPG7
Gene names
Name:ATG7
Synonyms:APG7
Ordered Locus Names:At5g45900
ORF Names:K15I22.10
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length697 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 and ATG8 for its conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes. Involved in the senescence process. Ref.1

Subunit structure

Homodimer. Interacts with ATG8 through a thioester bond between Cys-558 and the C-terminal glycine of ATG8 and with ATG12 through a thioester bond between Cys-558 and the C-terminal glycine of ATG12. Interacts also with ATG3 By similarity.

Tissue specificity

Constitutively expressed (at protein level). Ref.1

Developmental stage

Up-regulated during leaf senescence. Ref.1

Domain

The C-terminal residues are required for homodimerization, as well as the interactions with ATG3, ATG8 and ATG12 By similarity.

The GxGxxG motif is important for the function, possibly through binding with ATP.

Sequence similarities

Belongs to the ATG7 family.

Sequence caution

The sequence BAB09318.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 697697Ubiquitin-like modifier-activating enzyme atg7
PRO_0000286939

Regions

Region656 – 69742Homodimerization By similarity
Motif364 – 3696GXGXXG motif
Compositional bias685 – 6928Poly-Asp

Sites

Active site5581Glycyl thioester intermediate By similarity

Secondary structure

................................................... 697
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q94CD5 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: F9855AED0F9007FB

FASTA69776,522
        10         20         30         40         50         60 
MAEKETPAII LQFAPLNSSV DEGFWHSFSS LKLDKLGIDD SPISITGFYG PCGHPQVSNH 

        70         80         90        100        110        120 
LTLLSESLPL DEQSLIASTS HGNRNKCPVP GILYNTNTVE SFNKLDKQSL LKAEANKIWE 

       130        140        150        160        170        180 
DIQSGKALED PSVLPRFLVI SFADLKKWSF RYWFAFPAFV LDPPVSLIEL KPASEYFSSE 

       190        200        210        220        230        240 
EAESVSAACN DWRDSDLTTD VPFFLVSVSS DSKASIRHLK DLEACQGDHQ KLLFGFYDPC 

       250        260        270        280        290        300 
HLPSNPGWPL RNYLALIRSR WNLETVWFFC YRESRGFADL NLSLVGQASI TLSSGESAET 

       310        320        330        340        350        360 
VPNSVGWELN KGKRVPRSIS LANSMDPTRL AVSAVDLNLK LMRWRALPSL NLNVLSSVKC 

       370        380        390        400        410        420 
LLLGAGTLGC QVARTLMGWG IRNITFVDYG KVAMSNPVRQ SLYNFEDCLG RGEFKAVAAV 

       430        440        450        460        470        480 
KSLKQIFPAM ETSGVVMAIP MPGHPISSQE EDSVLGDCKR LSELIESHDA VFLLTDTRES 

       490        500        510        520        530        540 
RWLPSLLCAN ANKIAINAAL GFDSYMVMRH GAGPTSLSDD MQNLDINKTN TQRLGCYFCN 

       550        560        570        580        590        600 
DVVAPQDSMT DRTLDQQCTV TRPGLAPIAG ALAVELLVGV LQHPLGINAK GDNSSLSNTG 

       610        620        630        640        650        660 
NNDDSPLGIL PHQIRGSVSQ FSQITLLGQA SNSCTACSET VISEYRERGN SFILEAINHP 

       670        680        690 
TYLEDLTGLT ELKKAANSFN LDWEDDDTDD DDVAVDL 

« Hide

References

« Hide 'large scale' references
[1]"The APG8/12-activating enzyme APG7 is required for proper nutrient recycling and senescence in Arabidopsis thaliana."
Doelling J.H., Walker J.M., Friedman E.M., Thompson A.R., Vierstra R.D.
J. Biol. Chem. 277:33105-33114(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.
Strain: cv. Columbia.
[2]"Leaf senescence and starvation-induced chlorosis are accelerated by the disruption of an Arabidopsis autophagy gene."
Hanaoka H., Noda T., Shirano Y., Kato T., Hayashi H., Shibata D., Tabata S., Ohsumi Y.
Plant Physiol. 129:1181-1193(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], NOMENCLATURE, GENE FAMILY.
[3]"Structural analysis of Arabidopsis thaliana chromosome 5. VIII. Sequence features of the regions of 1,081,958 bp covered by seventeen physically assigned P1 and TAC clones."
Asamizu E., Sato S., Kaneko T., Nakamura Y., Kotani H., Miyajima N., Tabata S.
DNA Res. 5:379-391(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[4]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[5]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF492761 mRNA. Translation: AAM70190.1.
AB073173 mRNA. Translation: BAB88385.1.
AB016870 Genomic DNA. Translation: BAB09318.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED95313.1.
AY034945 mRNA. Translation: AAK59451.1.
AY150456 mRNA. Translation: AAN12897.1.
RefSeqNP_568652.1. NM_123958.2.
UniGeneAt.7711.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3VX8X-ray3.11A/D7-325[»]
ProteinModelPortalQ94CD5.
SMRQ94CD5. Positions 7-674.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid19879. 4 interactions.
IntActQ94CD5. 3 interactions.
MINTMINT-8414464.
STRING3702.AT5G45900.1-P.

Proteomic databases

PaxDbQ94CD5.
PRIDEQ94CD5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G45900.1; AT5G45900.1; AT5G45900.
GeneID834630.
KEGGath:AT5G45900.

Organism-specific databases

TAIRAT5G45900.

Phylogenomic databases

eggNOGCOG0476.
HOGENOMHOG000162379.
InParanoidQ94CD5.
KOK08337.
OMAMRWRALP.
PhylomeDBQ94CD5.
ProtClustDBCLSN2689937.

Gene expression databases

GenevestigatorQ94CD5.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
InterProIPR006285. Atg7.
IPR009036. Molybdenum_cofac_synth_MoeB.
IPR016040. NAD(P)-bd_dom.
IPR000594. ThiF_NAD_FAD-bd.
[Graphical view]
PANTHERPTHR10953:SF3. PTHR10953:SF3. 1 hit.
PfamPF00899. ThiF. 1 hit.
[Graphical view]
SUPFAMSSF69572. SSF69572. 1 hit.
TIGRFAMsTIGR01381. E1_like_apg7. 1 hit.
ProtoNetSearch...

Other

PROQ94CD5.

Entry information

Entry nameATG7_ARATH
AccessionPrimary (citable) accession number: Q94CD5
Secondary accession number(s): Q9FJ46
Entry history
Integrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: December 1, 2001
Last modified: February 19, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names