Q94CD5 (ATG7_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin-like modifier-activating enzyme atg7

Alternative name(s):
ATG12-activating enzyme E1 atg7
Autophagy-related protein 7
Short name=AtAPG7

Gene names

Name:	ATG7
Synonyms:	APG7
Ordered Locus Names:	At5g45900
ORF Names:	K15I22.10

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	697 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 and ATG8 for its conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes. Involved in the senescence process. Ref.1
Subunit structure	Homodimer. Interacts with ATG8 through a thioester bond between Cys-558 and the C-terminal glycine of ATG8 and with ATG12 through a thioester bond between Cys-558 and the C-terminal glycine of ATG12. Interacts also with ATG3 By similarity.
Tissue specificity	Constitutively expressed (at protein level). Ref.1
Developmental stage	Up-regulated during leaf senescence. Ref.1
Domain	The C-terminal residues are required for homodimerization, as well as the interactions with ATG3, ATG8 and ATG12 By similarity. The GxGxxG motif is important for the function, possibly through binding with ATP.
Sequence similarities	Belongs to the ATG7 family.
Sequence caution	The sequence BAB09318.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Autophagy Protein transport Transport Ubl conjugation pathway
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	autophagy Inferred from sequence or structural similarity Ref.1. Source: TAIR defense response to fungus Inferred from expression pattern PubMed 21395886. Source: TAIR leaf senescence Inferred from mutant phenotype PubMed 16040659. Source: TAIR protein lipidation Inferred from sequence or structural similarity Ref.1. Source: TAIR protein transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytosol Inferred from direct assay PubMed 21166475. Source: TAIR
Molecular_function	APG8 activating enzyme activity Inferred from sequence or structural similarity Ref.1. Source: TAIR nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 697

697

Ubiquitin-like modifier-activating enzyme atg7

PRO_0000286939

Regions

Region

656 – 697

42

Homodimerization By similarity

Motif

364 – 369

6

GXGXXG motif

Compositional bias

685 – 692

8

Poly-Asp

Sites

Active site

558

1

Glycyl thioester intermediate By similarity

Secondary structure

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697

Helix Strand Turn

Details...

Beta strand

18 – 20

3

Helix

22 – 34

13

Turn

35 – 38

4

Beta strand

43 – 49

7

Beta strand

61 – 63

3

Helix

65 – 67

3

Beta strand

86 – 98

13

Helix

99 – 104

6

Helix

108 – 123

16

Helix

126 – 129

4

Helix

131 – 133

3

Beta strand

137 – 144

8

Turn

145 – 148

4

Beta strand

149 – 159

11

Beta strand

166 – 172

7

Helix

173 – 176

4

Helix

179 – 194

16

Beta strand

203 – 209

7

Beta strand

214 – 217

4

Helix

219 – 221

3

Helix

222 – 225

4

Beta strand

230 – 237

8

Helix

250 – 261

12

Beta strand

264 – 271

8

Turn

280 – 282

3

Beta strand

284 – 289

6

Beta strand

304 – 307

4

Beta strand

317 – 320

4

Sequences

	Sequence		Length	Mass (Da)
	Q94CD5 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: F9855AED0F9007FB	FASTA	697	76,522
	10 20 30 40 50 60 MAEKETPAII LQFAPLNSSV DEGFWHSFSS LKLDKLGIDD SPISITGFYG PCGHPQVSNH 70 80 90 100 110 120 LTLLSESLPL DEQSLIASTS HGNRNKCPVP GILYNTNTVE SFNKLDKQSL LKAEANKIWE 130 140 150 160 170 180 DIQSGKALED PSVLPRFLVI SFADLKKWSF RYWFAFPAFV LDPPVSLIEL KPASEYFSSE 190 200 210 220 230 240 EAESVSAACN DWRDSDLTTD VPFFLVSVSS DSKASIRHLK DLEACQGDHQ KLLFGFYDPC 250 260 270 280 290 300 HLPSNPGWPL RNYLALIRSR WNLETVWFFC YRESRGFADL NLSLVGQASI TLSSGESAET 310 320 330 340 350 360 VPNSVGWELN KGKRVPRSIS LANSMDPTRL AVSAVDLNLK LMRWRALPSL NLNVLSSVKC 370 380 390 400 410 420 LLLGAGTLGC QVARTLMGWG IRNITFVDYG KVAMSNPVRQ SLYNFEDCLG RGEFKAVAAV 430 440 450 460 470 480 KSLKQIFPAM ETSGVVMAIP MPGHPISSQE EDSVLGDCKR LSELIESHDA VFLLTDTRES 490 500 510 520 530 540 RWLPSLLCAN ANKIAINAAL GFDSYMVMRH GAGPTSLSDD MQNLDINKTN TQRLGCYFCN 550 560 570 580 590 600 DVVAPQDSMT DRTLDQQCTV TRPGLAPIAG ALAVELLVGV LQHPLGINAK GDNSSLSNTG 610 620 630 640 650 660 NNDDSPLGIL PHQIRGSVSQ FSQITLLGQA SNSCTACSET VISEYRERGN SFILEAINHP 670 680 690 TYLEDLTGLT ELKKAANSFN LDWEDDDTDD DDVAVDL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The APG8/12-activating enzyme APG7 is required for proper nutrient recycling and senescence in Arabidopsis thaliana." Doelling J.H., Walker J.M., Friedman E.M., Thompson A.R., Vierstra R.D. J. Biol. Chem. 277:33105-33114(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY. Strain: cv. Columbia.
[2]	"Leaf senescence and starvation-induced chlorosis are accelerated by the disruption of an Arabidopsis autophagy gene." Hanaoka H., Noda T., Shirano Y., Kato T., Hayashi H., Shibata D., Tabata S., Ohsumi Y. Plant Physiol. 129:1181-1193(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], NOMENCLATURE, GENE FAMILY.
[3]	"Structural analysis of Arabidopsis thaliana chromosome 5. VIII. Sequence features of the regions of 1,081,958 bp covered by seventeen physically assigned P1 and TAC clones." Asamizu E., Sato S., Kaneko T., Nakamura Y., Kotani H., Miyajima N., Tabata S. DNA Res. 5:379-391(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[5]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF492761 mRNA. Translation: AAM70190.1.
AB073173 mRNA. Translation: BAB88385.1.
AB016870 Genomic DNA. Translation: BAB09318.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED95313.1.
AY034945 mRNA. Translation: AAK59451.1.
AY150456 mRNA. Translation: AAN12897.1.

IPI

IPI00547970.

RefSeq

NP_568652.1. NM_123958.2.

UniGene

At.7711.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3VX8	X-ray	3.11	A/D	7-325	[»]

ProteinModelPortal

Q94CD5.

SMR

Q94CD5. Positions 7-674.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q94CD5. 2 interactions.

STRING

3702.AT5G45900.1-P.

Proteomic databases

PaxDb

Q94CD5.

PRIDE

Q94CD5.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblPlants

AT5G45900.1; AT5G45900.1; AT5G45900.

GeneID

834630.

KEGG

ath:AT5G45900.

Organism-specific databases

TAIR

At5g45900.

Phylogenomic databases

eggNOG

COG0476.

HOGENOM

HOG000162379.

InParanoid

Q94CD5.

KO

K08337.

OMA

MRWRALP.

PhylomeDB

Q94CD5.

ProtClustDB

CLSN2689937.

Gene expression databases

Genevestigator

Q94CD5.

Family and domain databases

Gene3D

3.40.50.720. 1 hit.

InterPro

IPR006285. Atg7.
IPR009036. Molybdenum_cofac_synth_MoeB.
IPR016040. NAD(P)-bd_dom.
IPR000594. ThiF_NAD_FAD-bd.
[Graphical view]

PANTHER

PTHR10953:SF3. PTHR10953:SF3. 1 hit.

Pfam

PF00899. ThiF. 1 hit.
[Graphical view]

SUPFAM

SSF69572. MoeB. 1 hit.

TIGRFAMs

TIGR01381. E1_like_apg7. 1 hit.

ProtoNet

Search...

Entry information

Entry name

ATG7_ARATH

Accession

Primary (citable) accession number: Q94CD5
Secondary accession number(s): Q9FJ46

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 15, 2007
Last sequence update:	December 1, 2001
Last modified:	May 1, 2013
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program