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Q94CD5

- ATG7_ARATH

UniProt

Q94CD5 - ATG7_ARATH

Protein

Ubiquitin-like modifier-activating enzyme atg7

Gene

ATG7

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 84 (01 Oct 2014)
      Sequence version 1 (01 Dec 2001)
      Previous versions | rss
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    Functioni

    E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 and ATG8 for its conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes. Involved in the senescence process.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei558 – 5581Glycyl thioester intermediateBy similarity

    GO - Molecular functioni

    1. Atg8 activating enzyme Source: TAIR

    GO - Biological processi

    1. autophagy Source: TAIR
    2. defense response to fungus Source: TAIR
    3. leaf senescence Source: TAIR
    4. protein lipidation Source: TAIR
    5. protein transport Source: UniProtKB-KW

    Keywords - Biological processi

    Autophagy, Protein transport, Transport, Ubl conjugation pathway

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin-like modifier-activating enzyme atg7
    Alternative name(s):
    ATG12-activating enzyme E1 atg7
    Autophagy-related protein 7
    Short name:
    AtAPG7
    Gene namesi
    Name:ATG7
    Synonyms:APG7
    Ordered Locus Names:At5g45900
    ORF Names:K15I22.10
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G45900.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: TAIR

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 697697Ubiquitin-like modifier-activating enzyme atg7PRO_0000286939Add
    BLAST

    Proteomic databases

    PaxDbiQ94CD5.
    PRIDEiQ94CD5.

    Expressioni

    Tissue specificityi

    Constitutively expressed (at protein level).1 Publication

    Developmental stagei

    Up-regulated during leaf senescence.1 Publication

    Gene expression databases

    GenevestigatoriQ94CD5.

    Interactioni

    Subunit structurei

    Homodimer. Interacts with ATG8 through a thioester bond between Cys-558 and the C-terminal glycine of ATG8 and with ATG12 through a thioester bond between Cys-558 and the C-terminal glycine of ATG12. Interacts also with ATG3 By similarity.By similarity

    Protein-protein interaction databases

    BioGridi19879. 4 interactions.
    IntActiQ94CD5. 3 interactions.
    MINTiMINT-8414464.
    STRINGi3702.AT5G45900.1-P.

    Structurei

    Secondary structure

    1
    697
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi18 – 203
    Helixi22 – 3413
    Turni35 – 384
    Beta strandi43 – 497
    Beta strandi61 – 633
    Helixi65 – 673
    Beta strandi86 – 9813
    Helixi99 – 1046
    Helixi108 – 12316
    Helixi126 – 1294
    Helixi131 – 1333
    Beta strandi137 – 1448
    Turni145 – 1484
    Beta strandi149 – 15911
    Beta strandi166 – 1727
    Helixi173 – 1764
    Helixi179 – 19416
    Beta strandi203 – 2097
    Beta strandi214 – 2174
    Helixi219 – 2213
    Helixi222 – 2254
    Beta strandi230 – 2378
    Helixi250 – 26112
    Beta strandi264 – 2718
    Turni280 – 2823
    Beta strandi284 – 2896
    Beta strandi304 – 3074
    Beta strandi317 – 3204

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3VX8X-ray3.11A/D7-325[»]
    ProteinModelPortaliQ94CD5.
    SMRiQ94CD5. Positions 326-674.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni656 – 69742HomodimerizationBy similarityAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi364 – 3696GXGXXG motif

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi685 – 6928Poly-Asp

    Domaini

    The C-terminal residues are required for homodimerization, as well as the interactions with ATG3, ATG8 and ATG12.By similarity
    The GxGxxG motif is important for the function, possibly through binding with ATP.

    Sequence similaritiesi

    Belongs to the ATG7 family.Curated

    Phylogenomic databases

    eggNOGiCOG0476.
    HOGENOMiHOG000162379.
    InParanoidiQ94CD5.
    KOiK08337.
    OMAiLGCQVAR.
    PhylomeDBiQ94CD5.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR006285. Atg7.
    IPR009036. Molybdenum_cofac_synth_MoeB.
    IPR016040. NAD(P)-bd_dom.
    IPR000594. ThiF_NAD_FAD-bd.
    [Graphical view]
    PANTHERiPTHR10953:SF3. PTHR10953:SF3. 1 hit.
    PfamiPF00899. ThiF. 1 hit.
    [Graphical view]
    SUPFAMiSSF69572. SSF69572. 1 hit.
    TIGRFAMsiTIGR01381. E1_like_apg7. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q94CD5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAEKETPAII LQFAPLNSSV DEGFWHSFSS LKLDKLGIDD SPISITGFYG    50
    PCGHPQVSNH LTLLSESLPL DEQSLIASTS HGNRNKCPVP GILYNTNTVE 100
    SFNKLDKQSL LKAEANKIWE DIQSGKALED PSVLPRFLVI SFADLKKWSF 150
    RYWFAFPAFV LDPPVSLIEL KPASEYFSSE EAESVSAACN DWRDSDLTTD 200
    VPFFLVSVSS DSKASIRHLK DLEACQGDHQ KLLFGFYDPC HLPSNPGWPL 250
    RNYLALIRSR WNLETVWFFC YRESRGFADL NLSLVGQASI TLSSGESAET 300
    VPNSVGWELN KGKRVPRSIS LANSMDPTRL AVSAVDLNLK LMRWRALPSL 350
    NLNVLSSVKC LLLGAGTLGC QVARTLMGWG IRNITFVDYG KVAMSNPVRQ 400
    SLYNFEDCLG RGEFKAVAAV KSLKQIFPAM ETSGVVMAIP MPGHPISSQE 450
    EDSVLGDCKR LSELIESHDA VFLLTDTRES RWLPSLLCAN ANKIAINAAL 500
    GFDSYMVMRH GAGPTSLSDD MQNLDINKTN TQRLGCYFCN DVVAPQDSMT 550
    DRTLDQQCTV TRPGLAPIAG ALAVELLVGV LQHPLGINAK GDNSSLSNTG 600
    NNDDSPLGIL PHQIRGSVSQ FSQITLLGQA SNSCTACSET VISEYRERGN 650
    SFILEAINHP TYLEDLTGLT ELKKAANSFN LDWEDDDTDD DDVAVDL 697
    Length:697
    Mass (Da):76,522
    Last modified:December 1, 2001 - v1
    Checksum:iF9855AED0F9007FB
    GO

    Sequence cautioni

    The sequence BAB09318.1 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF492761 mRNA. Translation: AAM70190.1.
    AB073173 mRNA. Translation: BAB88385.1.
    AB016870 Genomic DNA. Translation: BAB09318.1. Sequence problems.
    CP002688 Genomic DNA. Translation: AED95313.1.
    AY034945 mRNA. Translation: AAK59451.1.
    AY150456 mRNA. Translation: AAN12897.1.
    RefSeqiNP_568652.1. NM_123958.2.
    UniGeneiAt.7711.

    Genome annotation databases

    EnsemblPlantsiAT5G45900.1; AT5G45900.1; AT5G45900.
    GeneIDi834630.
    KEGGiath:AT5G45900.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF492761 mRNA. Translation: AAM70190.1 .
    AB073173 mRNA. Translation: BAB88385.1 .
    AB016870 Genomic DNA. Translation: BAB09318.1 . Sequence problems.
    CP002688 Genomic DNA. Translation: AED95313.1 .
    AY034945 mRNA. Translation: AAK59451.1 .
    AY150456 mRNA. Translation: AAN12897.1 .
    RefSeqi NP_568652.1. NM_123958.2.
    UniGenei At.7711.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3VX8 X-ray 3.11 A/D 7-325 [» ]
    ProteinModelPortali Q94CD5.
    SMRi Q94CD5. Positions 326-674.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 19879. 4 interactions.
    IntActi Q94CD5. 3 interactions.
    MINTi MINT-8414464.
    STRINGi 3702.AT5G45900.1-P.

    Proteomic databases

    PaxDbi Q94CD5.
    PRIDEi Q94CD5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G45900.1 ; AT5G45900.1 ; AT5G45900 .
    GeneIDi 834630.
    KEGGi ath:AT5G45900.

    Organism-specific databases

    TAIRi AT5G45900.

    Phylogenomic databases

    eggNOGi COG0476.
    HOGENOMi HOG000162379.
    InParanoidi Q94CD5.
    KOi K08337.
    OMAi LGCQVAR.
    PhylomeDBi Q94CD5.

    Miscellaneous databases

    PROi Q94CD5.

    Gene expression databases

    Genevestigatori Q94CD5.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    InterProi IPR006285. Atg7.
    IPR009036. Molybdenum_cofac_synth_MoeB.
    IPR016040. NAD(P)-bd_dom.
    IPR000594. ThiF_NAD_FAD-bd.
    [Graphical view ]
    PANTHERi PTHR10953:SF3. PTHR10953:SF3. 1 hit.
    Pfami PF00899. ThiF. 1 hit.
    [Graphical view ]
    SUPFAMi SSF69572. SSF69572. 1 hit.
    TIGRFAMsi TIGR01381. E1_like_apg7. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The APG8/12-activating enzyme APG7 is required for proper nutrient recycling and senescence in Arabidopsis thaliana."
      Doelling J.H., Walker J.M., Friedman E.M., Thompson A.R., Vierstra R.D.
      J. Biol. Chem. 277:33105-33114(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.
      Strain: cv. Columbia.
    2. "Leaf senescence and starvation-induced chlorosis are accelerated by the disruption of an Arabidopsis autophagy gene."
      Hanaoka H., Noda T., Shirano Y., Kato T., Hayashi H., Shibata D., Tabata S., Ohsumi Y.
      Plant Physiol. 129:1181-1193(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], NOMENCLATURE, GENE FAMILY.
    3. "Structural analysis of Arabidopsis thaliana chromosome 5. VIII. Sequence features of the regions of 1,081,958 bp covered by seventeen physically assigned P1 and TAC clones."
      Asamizu E., Sato S., Kaneko T., Nakamura Y., Kotani H., Miyajima N., Tabata S.
      DNA Res. 5:379-391(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    4. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.

    Entry informationi

    Entry nameiATG7_ARATH
    AccessioniPrimary (citable) accession number: Q94CD5
    Secondary accession number(s): Q9FJ46
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 15, 2007
    Last sequence update: December 1, 2001
    Last modified: October 1, 2014
    This is version 84 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3