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Q94CD5

- ATG7_ARATH

UniProt

Q94CD5 - ATG7_ARATH

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Protein

Ubiquitin-like modifier-activating enzyme atg7

Gene
ATG7, APG7, At5g45900, K15I22.10
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 and ATG8 for its conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes. Involved in the senescence process.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei558 – 5581Glycyl thioester intermediate By similarity

GO - Molecular functioni

  1. Atg8 activating enzyme Source: TAIR

GO - Biological processi

  1. autophagy Source: TAIR
  2. defense response to fungus Source: TAIR
  3. leaf senescence Source: TAIR
  4. protein lipidation Source: TAIR
  5. protein transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Autophagy, Protein transport, Transport, Ubl conjugation pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-like modifier-activating enzyme atg7
Alternative name(s):
ATG12-activating enzyme E1 atg7
Autophagy-related protein 7
Short name:
AtAPG7
Gene namesi
Name:ATG7
Synonyms:APG7
Ordered Locus Names:At5g45900
ORF Names:K15I22.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G45900.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: TAIR
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 697697Ubiquitin-like modifier-activating enzyme atg7PRO_0000286939Add
BLAST

Proteomic databases

PaxDbiQ94CD5.
PRIDEiQ94CD5.

Expressioni

Tissue specificityi

Constitutively expressed (at protein level).1 Publication

Developmental stagei

Up-regulated during leaf senescence.1 Publication

Gene expression databases

GenevestigatoriQ94CD5.

Interactioni

Subunit structurei

Homodimer. Interacts with ATG8 through a thioester bond between Cys-558 and the C-terminal glycine of ATG8 and with ATG12 through a thioester bond between Cys-558 and the C-terminal glycine of ATG12. Interacts also with ATG3 By similarity.

Protein-protein interaction databases

BioGridi19879. 4 interactions.
IntActiQ94CD5. 3 interactions.
MINTiMINT-8414464.
STRINGi3702.AT5G45900.1-P.

Structurei

Secondary structure

1
697
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi18 – 203
Helixi22 – 3413
Turni35 – 384
Beta strandi43 – 497
Beta strandi61 – 633
Helixi65 – 673
Beta strandi86 – 9813
Helixi99 – 1046
Helixi108 – 12316
Helixi126 – 1294
Helixi131 – 1333
Beta strandi137 – 1448
Turni145 – 1484
Beta strandi149 – 15911
Beta strandi166 – 1727
Helixi173 – 1764
Helixi179 – 19416
Beta strandi203 – 2097
Beta strandi214 – 2174
Helixi219 – 2213
Helixi222 – 2254
Beta strandi230 – 2378
Helixi250 – 26112
Beta strandi264 – 2718
Turni280 – 2823
Beta strandi284 – 2896
Beta strandi304 – 3074
Beta strandi317 – 3204

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3VX8X-ray3.11A/D7-325[»]
ProteinModelPortaliQ94CD5.
SMRiQ94CD5. Positions 326-674.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni656 – 69742Homodimerization By similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi364 – 3696GXGXXG motif

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi685 – 6928Poly-Asp

Domaini

The C-terminal residues are required for homodimerization, as well as the interactions with ATG3, ATG8 and ATG12 By similarity.
The GxGxxG motif is important for the function, possibly through binding with ATP.

Sequence similaritiesi

Belongs to the ATG7 family.

Phylogenomic databases

eggNOGiCOG0476.
HOGENOMiHOG000162379.
InParanoidiQ94CD5.
KOiK08337.
OMAiLGCQVAR.
PhylomeDBiQ94CD5.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR006285. Atg7.
IPR009036. Molybdenum_cofac_synth_MoeB.
IPR016040. NAD(P)-bd_dom.
IPR000594. ThiF_NAD_FAD-bd.
[Graphical view]
PANTHERiPTHR10953:SF3. PTHR10953:SF3. 1 hit.
PfamiPF00899. ThiF. 1 hit.
[Graphical view]
SUPFAMiSSF69572. SSF69572. 1 hit.
TIGRFAMsiTIGR01381. E1_like_apg7. 1 hit.

Sequencei

Sequence statusi: Complete.

Q94CD5-1 [UniParc]FASTAAdd to Basket

« Hide

MAEKETPAII LQFAPLNSSV DEGFWHSFSS LKLDKLGIDD SPISITGFYG    50
PCGHPQVSNH LTLLSESLPL DEQSLIASTS HGNRNKCPVP GILYNTNTVE 100
SFNKLDKQSL LKAEANKIWE DIQSGKALED PSVLPRFLVI SFADLKKWSF 150
RYWFAFPAFV LDPPVSLIEL KPASEYFSSE EAESVSAACN DWRDSDLTTD 200
VPFFLVSVSS DSKASIRHLK DLEACQGDHQ KLLFGFYDPC HLPSNPGWPL 250
RNYLALIRSR WNLETVWFFC YRESRGFADL NLSLVGQASI TLSSGESAET 300
VPNSVGWELN KGKRVPRSIS LANSMDPTRL AVSAVDLNLK LMRWRALPSL 350
NLNVLSSVKC LLLGAGTLGC QVARTLMGWG IRNITFVDYG KVAMSNPVRQ 400
SLYNFEDCLG RGEFKAVAAV KSLKQIFPAM ETSGVVMAIP MPGHPISSQE 450
EDSVLGDCKR LSELIESHDA VFLLTDTRES RWLPSLLCAN ANKIAINAAL 500
GFDSYMVMRH GAGPTSLSDD MQNLDINKTN TQRLGCYFCN DVVAPQDSMT 550
DRTLDQQCTV TRPGLAPIAG ALAVELLVGV LQHPLGINAK GDNSSLSNTG 600
NNDDSPLGIL PHQIRGSVSQ FSQITLLGQA SNSCTACSET VISEYRERGN 650
SFILEAINHP TYLEDLTGLT ELKKAANSFN LDWEDDDTDD DDVAVDL 697
Length:697
Mass (Da):76,522
Last modified:December 1, 2001 - v1
Checksum:iF9855AED0F9007FB
GO

Sequence cautioni

The sequence BAB09318.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF492761 mRNA. Translation: AAM70190.1.
AB073173 mRNA. Translation: BAB88385.1.
AB016870 Genomic DNA. Translation: BAB09318.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED95313.1.
AY034945 mRNA. Translation: AAK59451.1.
AY150456 mRNA. Translation: AAN12897.1.
RefSeqiNP_568652.1. NM_123958.2.
UniGeneiAt.7711.

Genome annotation databases

EnsemblPlantsiAT5G45900.1; AT5G45900.1; AT5G45900.
GeneIDi834630.
KEGGiath:AT5G45900.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF492761 mRNA. Translation: AAM70190.1 .
AB073173 mRNA. Translation: BAB88385.1 .
AB016870 Genomic DNA. Translation: BAB09318.1 . Sequence problems.
CP002688 Genomic DNA. Translation: AED95313.1 .
AY034945 mRNA. Translation: AAK59451.1 .
AY150456 mRNA. Translation: AAN12897.1 .
RefSeqi NP_568652.1. NM_123958.2.
UniGenei At.7711.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3VX8 X-ray 3.11 A/D 7-325 [» ]
ProteinModelPortali Q94CD5.
SMRi Q94CD5. Positions 326-674.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 19879. 4 interactions.
IntActi Q94CD5. 3 interactions.
MINTi MINT-8414464.
STRINGi 3702.AT5G45900.1-P.

Proteomic databases

PaxDbi Q94CD5.
PRIDEi Q94CD5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT5G45900.1 ; AT5G45900.1 ; AT5G45900 .
GeneIDi 834630.
KEGGi ath:AT5G45900.

Organism-specific databases

TAIRi AT5G45900.

Phylogenomic databases

eggNOGi COG0476.
HOGENOMi HOG000162379.
InParanoidi Q94CD5.
KOi K08337.
OMAi LGCQVAR.
PhylomeDBi Q94CD5.

Miscellaneous databases

PROi Q94CD5.

Gene expression databases

Genevestigatori Q94CD5.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
InterProi IPR006285. Atg7.
IPR009036. Molybdenum_cofac_synth_MoeB.
IPR016040. NAD(P)-bd_dom.
IPR000594. ThiF_NAD_FAD-bd.
[Graphical view ]
PANTHERi PTHR10953:SF3. PTHR10953:SF3. 1 hit.
Pfami PF00899. ThiF. 1 hit.
[Graphical view ]
SUPFAMi SSF69572. SSF69572. 1 hit.
TIGRFAMsi TIGR01381. E1_like_apg7. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The APG8/12-activating enzyme APG7 is required for proper nutrient recycling and senescence in Arabidopsis thaliana."
    Doelling J.H., Walker J.M., Friedman E.M., Thompson A.R., Vierstra R.D.
    J. Biol. Chem. 277:33105-33114(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.
    Strain: cv. Columbia.
  2. "Leaf senescence and starvation-induced chlorosis are accelerated by the disruption of an Arabidopsis autophagy gene."
    Hanaoka H., Noda T., Shirano Y., Kato T., Hayashi H., Shibata D., Tabata S., Ohsumi Y.
    Plant Physiol. 129:1181-1193(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], NOMENCLATURE, GENE FAMILY.
  3. "Structural analysis of Arabidopsis thaliana chromosome 5. VIII. Sequence features of the regions of 1,081,958 bp covered by seventeen physically assigned P1 and TAC clones."
    Asamizu E., Sato S., Kaneko T., Nakamura Y., Kotani H., Miyajima N., Tabata S.
    DNA Res. 5:379-391(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.

Entry informationi

Entry nameiATG7_ARATH
AccessioniPrimary (citable) accession number: Q94CD5
Secondary accession number(s): Q9FJ46
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: December 1, 2001
Last modified: September 3, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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