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Protein

Ubiquitin-like modifier-activating enzyme atg7

Gene

ATG7

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 and ATG8 for its conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes. Involved in the senescence process (PubMed:12070171). Involved in the degradation of damaged peroxisomes (PubMed:24368788). Involved in the non-selective degradation of chlorophylls and photosynthetic proteins during stress-induced leaf yellowing (PubMed:24510943).3 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei558Glycyl thioester intermediateBy similarity1

GO - Molecular functioni

  • Atg12 activating enzyme activity Source: GO_Central
  • Atg8 activating enzyme activity Source: TAIR

GO - Biological processi

  • autophagy Source: TAIR
  • cellular response to nitrogen starvation Source: GO_Central
  • C-terminal protein lipidation Source: GO_Central
  • defense response to fungus Source: TAIR
  • late nucleophagy Source: GO_Central
  • leaf senescence Source: TAIR
  • macroautophagy Source: GO_Central
  • mitophagy Source: GO_Central
  • piecemeal microautophagy of nucleus Source: GO_Central
  • protein catabolic process Source: GO_Central
  • protein lipidation Source: TAIR
  • protein modification by small protein conjugation Source: GO_Central
  • protein transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Autophagy, Protein transport, Stress response, Transport, Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-ATH-1632852. Macroautophagy.
R-ATH-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-like modifier-activating enzyme atg7
Alternative name(s):
ATG12-activating enzyme E1 atg7
Autophagy-related protein 7
Short name:
AtAPG7
Protein PEROXISOME UNUSUAL POSITIONING 41 Publication
Gene namesi
Name:ATG7
Synonyms:APG7, PEUP41 Publication
Ordered Locus Names:At5g45900
ORF Names:K15I22.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G45900.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: TAIR
Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

Increased number of peroxisomes and accumulation of peroxisomal proteins.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi558C → Y in peup4; loss of function. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002869391 – 697Ubiquitin-like modifier-activating enzyme atg7Add BLAST697

Proteomic databases

PaxDbiQ94CD5.
PRIDEiQ94CD5.

Expressioni

Tissue specificityi

Constitutively expressed (at protein level).1 Publication

Developmental stagei

Up-regulated during leaf senescence.1 Publication

Gene expression databases

GenevisibleiQ94CD5. AT.

Interactioni

Subunit structurei

Homodimer. Interacts with ATG8 through a thioester bond between Cys-558 and the C-terminal glycine of ATG8 and with ATG12 through a thioester bond between Cys-558 and the C-terminal glycine of ATG12. Interacts also with ATG3 (By similarity).By similarity

Protein-protein interaction databases

BioGridi19879. 4 interactors.
IntActiQ94CD5. 3 interactors.
MINTiMINT-8414464.
STRINGi3702.AT5G45900.1.

Structurei

Secondary structure

1697
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi18 – 20Combined sources3
Helixi22 – 34Combined sources13
Turni35 – 38Combined sources4
Beta strandi43 – 49Combined sources7
Beta strandi61 – 63Combined sources3
Helixi65 – 67Combined sources3
Beta strandi86 – 98Combined sources13
Helixi99 – 104Combined sources6
Helixi108 – 123Combined sources16
Helixi126 – 129Combined sources4
Helixi131 – 133Combined sources3
Beta strandi137 – 144Combined sources8
Turni145 – 148Combined sources4
Beta strandi149 – 159Combined sources11
Beta strandi166 – 172Combined sources7
Helixi173 – 176Combined sources4
Helixi179 – 194Combined sources16
Beta strandi203 – 209Combined sources7
Beta strandi214 – 217Combined sources4
Helixi219 – 221Combined sources3
Helixi222 – 225Combined sources4
Beta strandi230 – 237Combined sources8
Helixi250 – 261Combined sources12
Beta strandi264 – 271Combined sources8
Turni280 – 282Combined sources3
Beta strandi284 – 289Combined sources6
Beta strandi304 – 307Combined sources4
Beta strandi317 – 320Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3VX8X-ray3.11A/D7-325[»]
ProteinModelPortaliQ94CD5.
SMRiQ94CD5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni656 – 697HomodimerizationBy similarityAdd BLAST42

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi364 – 369GXGXXG motif6

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi685 – 692Poly-Asp8

Domaini

The C-terminal residues are required for homodimerization, as well as the interactions with ATG3, ATG8 and ATG12.By similarity
The GxGxxG motif is important for the function, possibly through binding with ATP.

Sequence similaritiesi

Belongs to the ATG7 family.Curated

Phylogenomic databases

eggNOGiKOG2337. Eukaryota.
COG0476. LUCA.
HOGENOMiHOG000162379.
InParanoidiQ94CD5.
KOiK08337.
OMAiVMAIPMP.
OrthoDBiEOG093600ZU.
PhylomeDBiQ94CD5.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR006285. Atg7.
IPR032197. Atg7_N.
IPR016040. NAD(P)-bd_dom.
IPR000594. ThiF_NAD_FAD-bd.
[Graphical view]
PANTHERiPTHR10953:SF3. PTHR10953:SF3. 2 hits.
PfamiPF16420. ATG7_N. 1 hit.
PF00899. ThiF. 1 hit.
[Graphical view]
SUPFAMiSSF69572. SSF69572. 1 hit.
TIGRFAMsiTIGR01381. E1_like_apg7. 1 hit.

Sequencei

Sequence statusi: Complete.

Q94CD5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEKETPAII LQFAPLNSSV DEGFWHSFSS LKLDKLGIDD SPISITGFYG
60 70 80 90 100
PCGHPQVSNH LTLLSESLPL DEQSLIASTS HGNRNKCPVP GILYNTNTVE
110 120 130 140 150
SFNKLDKQSL LKAEANKIWE DIQSGKALED PSVLPRFLVI SFADLKKWSF
160 170 180 190 200
RYWFAFPAFV LDPPVSLIEL KPASEYFSSE EAESVSAACN DWRDSDLTTD
210 220 230 240 250
VPFFLVSVSS DSKASIRHLK DLEACQGDHQ KLLFGFYDPC HLPSNPGWPL
260 270 280 290 300
RNYLALIRSR WNLETVWFFC YRESRGFADL NLSLVGQASI TLSSGESAET
310 320 330 340 350
VPNSVGWELN KGKRVPRSIS LANSMDPTRL AVSAVDLNLK LMRWRALPSL
360 370 380 390 400
NLNVLSSVKC LLLGAGTLGC QVARTLMGWG IRNITFVDYG KVAMSNPVRQ
410 420 430 440 450
SLYNFEDCLG RGEFKAVAAV KSLKQIFPAM ETSGVVMAIP MPGHPISSQE
460 470 480 490 500
EDSVLGDCKR LSELIESHDA VFLLTDTRES RWLPSLLCAN ANKIAINAAL
510 520 530 540 550
GFDSYMVMRH GAGPTSLSDD MQNLDINKTN TQRLGCYFCN DVVAPQDSMT
560 570 580 590 600
DRTLDQQCTV TRPGLAPIAG ALAVELLVGV LQHPLGINAK GDNSSLSNTG
610 620 630 640 650
NNDDSPLGIL PHQIRGSVSQ FSQITLLGQA SNSCTACSET VISEYRERGN
660 670 680 690
SFILEAINHP TYLEDLTGLT ELKKAANSFN LDWEDDDTDD DDVAVDL
Length:697
Mass (Da):76,522
Last modified:December 1, 2001 - v1
Checksum:iF9855AED0F9007FB
GO

Sequence cautioni

The sequence BAB09318 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF492761 mRNA. Translation: AAM70190.1.
AB073173 mRNA. Translation: BAB88385.1.
AB016870 Genomic DNA. Translation: BAB09318.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED95313.1.
AY034945 mRNA. Translation: AAK59451.1.
AY150456 mRNA. Translation: AAN12897.1.
RefSeqiNP_568652.1. NM_123958.3.
UniGeneiAt.7711.

Genome annotation databases

EnsemblPlantsiAT5G45900.1; AT5G45900.1; AT5G45900.
GeneIDi834630.
GrameneiAT5G45900.1; AT5G45900.1; AT5G45900.
KEGGiath:AT5G45900.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF492761 mRNA. Translation: AAM70190.1.
AB073173 mRNA. Translation: BAB88385.1.
AB016870 Genomic DNA. Translation: BAB09318.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED95313.1.
AY034945 mRNA. Translation: AAK59451.1.
AY150456 mRNA. Translation: AAN12897.1.
RefSeqiNP_568652.1. NM_123958.3.
UniGeneiAt.7711.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3VX8X-ray3.11A/D7-325[»]
ProteinModelPortaliQ94CD5.
SMRiQ94CD5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi19879. 4 interactors.
IntActiQ94CD5. 3 interactors.
MINTiMINT-8414464.
STRINGi3702.AT5G45900.1.

Proteomic databases

PaxDbiQ94CD5.
PRIDEiQ94CD5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G45900.1; AT5G45900.1; AT5G45900.
GeneIDi834630.
GrameneiAT5G45900.1; AT5G45900.1; AT5G45900.
KEGGiath:AT5G45900.

Organism-specific databases

TAIRiAT5G45900.

Phylogenomic databases

eggNOGiKOG2337. Eukaryota.
COG0476. LUCA.
HOGENOMiHOG000162379.
InParanoidiQ94CD5.
KOiK08337.
OMAiVMAIPMP.
OrthoDBiEOG093600ZU.
PhylomeDBiQ94CD5.

Enzyme and pathway databases

ReactomeiR-ATH-1632852. Macroautophagy.
R-ATH-6798695. Neutrophil degranulation.

Miscellaneous databases

PROiQ94CD5.

Gene expression databases

GenevisibleiQ94CD5. AT.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR006285. Atg7.
IPR032197. Atg7_N.
IPR016040. NAD(P)-bd_dom.
IPR000594. ThiF_NAD_FAD-bd.
[Graphical view]
PANTHERiPTHR10953:SF3. PTHR10953:SF3. 2 hits.
PfamiPF16420. ATG7_N. 1 hit.
PF00899. ThiF. 1 hit.
[Graphical view]
SUPFAMiSSF69572. SSF69572. 1 hit.
TIGRFAMsiTIGR01381. E1_like_apg7. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiATG7_ARATH
AccessioniPrimary (citable) accession number: Q94CD5
Secondary accession number(s): Q9FJ46
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: December 1, 2001
Last modified: November 30, 2016
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.