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Q94BT6 (ADO1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 106. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Adagio protein 1
Alternative name(s):
Clock-associated PAS protein ZTL
F-box only protein 2b
Short name=FBX2b
Flavin-binding kelch repeat F-box protein 1-like protein 2
Short name=FKF1-like protein 2
LOV kelch protein 1
Protein ZEITLUPE
Gene names
Name:ADO1
Synonyms:FKL2, LKP1, ZTL
Ordered Locus Names:At5g57360
ORF Names:MSF19.2
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length609 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of an E3 ubiquitin ligase complex that plays a central role in blue light-dependent circadian cycles. Acts as a blue light photoreceptor, due to the presence of FMN, that mediates light-regulated protein degradation of critical clock components by targeting them to the proteasome complex. The SCF(ADO1) E3 ubiquitin ligase complex is involved in the regulation of circadian clock-dependent processes including the transition to flowering time, hypocotyl elongation, cotyledons and leaf movement rhythms. APRR1/TOC1 and APRR5, but not 'GIGANTEA', are proteolytic substrates of this ubiquitin ligase complex. Blue light enhances cooperative stabilization of 'GIGANTEA' and ADO1/ZTL, leading to amplification and sharpening of the expression profile of APRR1/TOC1. ADO1/ZTL interacts with ADO3, preventing the interaction of ADO3 with CDF1. Ref.1 Ref.2 Ref.3 Ref.4 Ref.12 Ref.13 Ref.14 Ref.15 Ref.17

Pathway

Protein modification; protein ubiquitination.

Subunit structure

Interacts with NFXL2. Interacts (via N-terminus) with GI and (via Kelch repeats) with ADO3. Component of an E3 ubiquitin ligase SCF(ADO1) complex composed of SKP1A/ASK1 (or SKP1B/ASK2), CUL1, RBX1 and ADO1. Also interacts with SKP1D/ASK4, SKP1K/ASK11, CRY1, PHYB, APRR1 and APRR5, and probably with SKP1N/ASK14 and SKP1S/ASK19. Ref.4 Ref.9 Ref.11 Ref.13 Ref.14 Ref.15 Ref.16 Ref.17 Ref.18

Subcellular location

Nucleus. Cytoplasm. Note: Nuclear after 9 hours of illumination (afternoon of long days). Cytoplasmic when plant have been subsequently grown 16 hours in light and 5 hours in dark (early morning of long days). Ref.3 Ref.15 Ref.17

Tissue specificity

Ubiquitously expressed with higher levels in cotyledons and leaves. Ref.2 Ref.3

Developmental stage

Mainly present during the light phase, and degraded in a proteasome-dependent manner in dark (at protein level).

Induction

Not regulated at the transcript level, but circadian-regulation at the protein level with a peak at the end of the subjective day. Ref.1 Ref.3 Ref.10 Ref.15 Ref.18

Post-translational modification

May be ubiquitinated. Degraded in a proteasome-dependent manner.

FMN binds covalently to cysteine after exposure to blue light and is reversed in the dark By similarity.

Disruption phenotype

Accumulation of ADO3 protein during the morning period and early flowering time. Ref.17

Miscellaneous

'Zeitlupe' means slow motion in German.

'Adagio' means slowly in Italian.

Sequence similarities

Belongs to the ADAGIO family.

Contains 1 F-box domain.

Contains 5 Kelch repeats.

Contains 1 PAC (PAS-associated C-terminal) domain.

Contains 1 PAS (PER-ARNT-SIM) domain.

Sequence caution

The sequence AAK64006.1 differs from that shown. Reason: Frameshift at positions 462 and 467.

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q94BT6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 609609Adagio protein 1
PRO_0000119956

Regions

Domain32 – 11483PAS
Domain118 – 16144PAC
Domain195 – 24147F-box
Repeat292 – 34251Kelch 1
Repeat345 – 39248Kelch 2
Repeat397 – 44549Kelch 3
Repeat450 – 50152Kelch 4
Repeat516 – 56449Kelch 5

Amino acid modifications

Modified residue821S-4a-FMN cysteine By similarity

Experimental info

Mutagenesis461G → E: Loss of binding to GI. Ref.15
Mutagenesis821C → A: Loss of binding to GI, but no effect on FNM binding. Ref.15
Mutagenesis1191G → D in ztl-21; long-period phenotype, decreased interaction with SKP1A/ASK1 and loss of binding to GI, NFXL2 and APRR1/TOC1, but no effect on binding to PHYB. Ref.14 Ref.15
Mutagenesis2001L → A: No SCF(ADO1) complex formation and reduced cyclic degradation of ADO1, but no effect on binding to GI; when associated with A-213. Ref.13 Ref.15
Mutagenesis2031E → K in ztl-22; long-period phenotype and loss of binding to NFXL2, but no effect on binding to GI or PHYB. Ref.14 Ref.15
Mutagenesis2131L → A: No SCF(ADO1) complex formation and reduced cyclic degradation of ADO1, but no effect on binding to GI; when associated with A-200. Ref.13 Ref.15
Mutagenesis2871G → D in ztl-23; long-period phenotype, but no effect on binding to PHYB. Ref.14
Mutagenesis3171P → S in ztl-24; long-period phenotype, but no effect on binding to PHYB. Ref.14
Mutagenesis3201D → N in ztl-2; affects circadian clock by lengthening the free-running period of clock-controlled processes. Ref.1 Ref.14
Mutagenesis3471G → S in ztl-25; long-period phenotype, but no effect on binding to PHYB. Ref.14
Mutagenesis3721D → N in ztl-26; long-period phenotype, but no effect on binding to PHYB. Ref.14
Mutagenesis4251D → N in ztl-1; affects circadian clock by lengthening the free-running period of clock-controlled processes, but has no effect on binding to GI or NFXL2. Ref.1 Ref.14 Ref.15
Mutagenesis4521G → D in ztl-27; long-period phenotype and loss of binding to APRR1/TOC1, but no effect on binding to GI or PHYB. Ref.14 Ref.15
Mutagenesis5641G → R in ztl-30; long-period phenotype, but no effect on binding to PHYB. Ref.14

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 8, 2005. Version 2.
Checksum: B25192FCBE019093

FASTA60965,906
        10         20         30         40         50         60 
MEWDSGSDLS ADDASSLADD EEGGLFPGGG PIPYPVGNLL HTAPCGFVVT DAVEPDQPII 

        70         80         90        100        110        120 
YVNTVFEMVT GYRAEEVLGG NCRFLQCRGP FAKRRHPLVD SMVVSEIRKC IDEGIEFQGE 

       130        140        150        160        170        180 
LLNFRKDGSP LMNRLRLTPI YGDDDTITHI IGIQFFIETD IDLGPVLGSS TKEKSIDGIY 

       190        200        210        220        230        240 
SALAAGERNV SRGMCGLFQL SDEVVSMKIL SRLTPRDVAS VSSVCRRLYV LTKNEDLWRR 

       250        260        270        280        290        300 
VCQNAWGSET TRVLETVPGA KRLGWGRLAR ELTTLEAAAW RKLSVGGSVE PSRCNFSACA 

       310        320        330        340        350        360 
VGNRVVLFGG EGVNMQPMND TFVLDLNSDY PEWQHVKVSS PPPGRWGHTL TCVNGSNLVV 

       370        380        390        400        410        420 
FGGCGQQGLL NDVFVLNLDA KPPTWREISG LAPPLPRSWH SSCTLDGTKL IVSGGCADSG 

       430        440        450        460        470        480 
VLLSDTFLLD LSIEKPVWRE IPAAWTPPSR LGHTLSVYGG RKILMFGGLA KSGPLKFRSS 

       490        500        510        520        530        540 
DVFTMDLSEE EPCWRCVTGS GMPGAGNPGG VAPPPRLDHV AVNLPGGRIL IFGGSVAGLH 

       550        560        570        580        590        600 
SASQLYLLDP TEDKPTWRIL NIPGRPPRFA WGHGTCVVGG TRAIVLGGQT GEEWMLSELH 


ELSLASYLT 

« Hide

References

« Hide 'large scale' references
[1]"ZEITLUPE encodes a novel clock-associated PAS protein from Arabidopsis."
Somers D.E., Schultz T.F., Milnamow M., Kay S.A.
Cell 101:319-329(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION, MUTAGENESIS OF ASP-320 AND ASP-425.
[2]"FKF1, a clock-controlled gene that regulates the transition to flowering in Arabidopsis."
Nelson D.C., Lasswell J.E., Rogg L.E., Cohen M.A., Bartel B.
Cell 101:331-340(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
[3]"LKP1 (LOV kelch protein 1): a factor involved in the regulation of flowering time in Arabidopsis."
Kiyosue T., Wada M.
Plant J. 23:807-815(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INDUCTION.
[4]"An Arabidopsis circadian clock component interacts with both CRY1 and phyB."
Jarillo J.A., Capel J., Tang R.-H., Yang H.-Q., Alonso J.M., Ecker J.R., Cashmore A.R.
Nature 410:487-490(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH CRY1 AND PHYB.
[5]"Structural analysis of Arabidopsis thaliana chromosome 5. VIII. Sequence features of the regions of 1,081,958 bp covered by seventeen physically assigned P1 and TAC clones."
Asamizu E., Sato S., Kaneko T., Nakamura Y., Kotani H., Miyajima N., Tabata S.
DNA Res. 5:379-391(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[6]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[7]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[8]"F-box proteins in Arabidopsis."
Xiao W., Jang J.-C.
Trends Plant Sci. 5:454-457(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
[9]"Protein interaction analysis of SCF ubiquitin E3 ligase subunits from Arabidopsis."
Risseeuw E.P., Daskalchuk T.E., Banks T.W., Liu E., Cotelesage J., Hellmann H., Estelle M., Somers D.E., Crosby W.L.
Plant J. 34:753-767(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SKP1A; SKP1B; SKP1D; SKP1K AND SKP1S.
[10]"Circadian phase-specific degradation of the F-box protein ZTL is mediated by the proteasome."
Kim W.-Y., Geng R., Somers D.E.
Proc. Natl. Acad. Sci. U.S.A. 100:4933-4938(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
[11]"Identification of ASK and clock-associated proteins as molecular partners of LKP2 (LOV kelch protein 2) in Arabidopsis."
Yasuhara M., Mitsui S., Hirano H., Takanabe R., Tokioka Y., Ihara N., Komatsu A., Seki M., Shinozaki K., Kiyosue T.
J. Exp. Bot. 55:2015-2027(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SKP1A; SKP1B; SKP1D; SKP1K; SKP1N; ADO2; APRR1 AND APRR5.
[12]"The F-box protein ZEITLUPE confers dosage-dependent control on the circadian clock, photomorphogenesis, and flowering time."
Somers D.E., Kim W.-Y., Geng R.
Plant Cell 16:769-782(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[13]"Formation of an SCF(ZTL) complex is required for proper regulation of circadian timing."
Han L., Mason M., Risseeuw E.P., Crosby W.L., Somers D.E.
Plant J. 40:291-301(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, MUTAGENESIS OF LEU-200 AND LEU-213, IDENTIFICATION IN A SCF(ADO1) COMPLEX.
[14]"Forward genetic analysis of the circadian clock separates the multiple functions of ZEITLUPE."
Kevei E., Gyula P., Hall A., Kozma-Bognar L., Kim W.Y., Eriksson M.E., Toth R., Hanano S., Feher B., Southern M.M., Bastow R.M., Viczian A., Hibberd V., Davis S.J., Somers D.E., Nagy F., Millar A.J.
Plant Physiol. 140:933-945(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, MUTAGENESIS OF GLY-119; GLU-203; GLY-287; PRO-317; ASP-320; GLY-347; ASP-372; ASP-425; GLY-452 AND GLY-564, INTERACTION WITH SKP1A; APRR1 AND PHYB.
[15]"ZEITLUPE is a circadian photoreceptor stabilized by GIGANTEA in blue light."
Kim W.Y., Fujiwara S., Suh S.S., Kim J., Kim Y., Han L., David K., Putterill J., Nam H.G., Somers D.E.
Nature 449:356-360(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, MUTAGENESIS OF GLY-46; CYS-82; GLY-119; LEU-200; GLU-203; LEU-213; ASP-425 AND GLY-452, INTERACTION WITH GI AND APRR1, INDUCTION, SUBCELLULAR LOCATION.
[16]"Post-translational regulation of the Arabidopsis circadian clock through selective proteolysis and phosphorylation of pseudo-response regulator proteins."
Fujiwara S., Wang L., Han L., Suh S.-S., Salome P.A., McClung C.R., Somers D.E.
J. Biol. Chem. 283:23073-23083(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH APRR5.
[17]"LOV KELCH PROTEIN2 and ZEITLUPE repress Arabidopsis photoperiodic flowering under non-inductive conditions, dependent on FLAVIN-BINDING KELCH REPEAT F-BOX1."
Takase T., Nishiyama Y., Tanihigashi H., Ogura Y., Miyazaki Y., Yamada Y., Kiyosue T.
Plant J. 67:608-621(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH ADO3, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
[18]"Partners in time: EARLY BIRD associates with ZEITLUPE and regulates the speed of the Arabidopsis clock."
Johansson M., McWatters H.G., Bako L., Takata N., Gyula P., Hall A., Somers D.E., Millar A.J., Eriksson M.E.
Plant Physiol. 155:2108-2122(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH NFXL2, INDUCTION.
+Additional computationally mapped references.

Web resources

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF254413 mRNA. Translation: AAF70288.1.
AF216525 mRNA. Translation: AAF32300.1.
AB038796 mRNA. Translation: BAB18914.1.
AF252294 mRNA. Translation: AAK27433.1.
AB016891 Genomic DNA. Translation: BAB08473.1.
CP002688 Genomic DNA. Translation: AED96891.1.
AY039902 mRNA. Translation: AAK64006.1. Frameshift.
BT008772 mRNA. Translation: AAP68211.1.
RefSeqNP_568855.1. NM_125119.3. [Q94BT6-1]
UniGeneAt.21022.

3D structure databases

ProteinModelPortalQ94BT6.
SMRQ94BT6. Positions 47-154, 273-354.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid21086. 23 interactions.
DIPDIP-32989N.
IntActQ94BT6. 20 interactions.
MINTMINT-192373.
STRING3702.AT5G57360.2-P.

Proteomic databases

PRIDEQ94BT6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G57360.1; AT5G57360.1; AT5G57360. [Q94BT6-1]
GeneID835842.
KEGGath:AT5G57360.

Organism-specific databases

GeneFarm5106. 485.
TAIRAT5G57360.

Phylogenomic databases

eggNOGCOG2202.
HOGENOMHOG000238789.
InParanoidQ94BT6.
KOK12115.
PhylomeDBQ94BT6.

Enzyme and pathway databases

UniPathwayUPA00143.

Gene expression databases

GenevestigatorQ94BT6.

Family and domain databases

Gene3D2.120.10.80. 2 hits.
InterProIPR001810. F-box_dom.
IPR011043. Gal_Oxase/kelch_b-propeller.
IPR015915. Kelch-typ_b-propeller.
IPR011498. Kelch_2.
IPR001610. PAC.
IPR000014. PAS.
[Graphical view]
PfamPF12937. F-box-like. 1 hit.
PF07646. Kelch_2. 1 hit.
PF13426. PAS_9. 1 hit.
[Graphical view]
SMARTSM00256. FBOX. 1 hit.
SM00086. PAC. 1 hit.
SM00091. PAS. 1 hit.
[Graphical view]
SUPFAMSSF50965. SSF50965. 1 hit.
SSF55785. SSF55785. 1 hit.
SSF81383. SSF81383. 1 hit.
TIGRFAMsTIGR00229. sensory_box. 1 hit.
PROSITEPS50181. FBOX. 1 hit.
PS50112. PAS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameADO1_ARATH
AccessionPrimary (citable) accession number: Q94BT6
Secondary accession number(s): Q9LDF6
Entry history
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: November 8, 2005
Last modified: June 11, 2014
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names